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A method for detecting bisphenol-a based on quantum dot-gold nano-assembled superstructure

A quantum dot and gold nanotechnology, which is applied in the field of detection of bisphenol A based on quantum dot-gold nanoassembly superstructure, can solve the problems of low controllability and difficulty in directional assembly of quantum dots, avoiding interference and improving specificity. Effects of Sex and Sensitivity

Active Publication Date: 2017-11-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of difficult directional assembly and low controllability of quantum dots in the prior art, and provide a controllable preparation method of quantum dot-gold nano-assembly superstructure based on DNA molecular template, and based on the nano-assembly Fluorescent sensor developed for rapid detection of bisphenol A with special optical properties

Method used

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  • A method for detecting bisphenol-a based on quantum dot-gold nano-assembled superstructure
  • A method for detecting bisphenol-a based on quantum dot-gold nano-assembled superstructure
  • A method for detecting bisphenol-a based on quantum dot-gold nano-assembled superstructure

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Embodiment 1

[0043] (1) Synthesis of gold nanoparticles

[0044] The preparation method of gold nanoparticles: take a clean Erlenmeyer flask, add 190mL ultrapure water, 10mL 0.2% chloroauric acid solution in turn, heat and stir the solution until boiling, and quickly add 3.6mL 1% trisodium citrate after 7-10min Solution, after the solution turns from colorless to red, stop heating and continue to stir for 15 minutes to make gold nanoparticles, and the concentration of gold nanoparticles is 10nM at this time.

[0045] (2) Synthesis of quantum dots

[0046] Take a clean Erlenmeyer flask, add 390mg Te powder, 189mg sodium borohydride, 4mL ultrapure water in turn, stir at room temperature for 5h until the black Te powder disappears completely, and white sodium borate crystals are produced. Under the protection of nitrogen, mix with H produced by the reaction of 0.5M dilute sulfuric acid 2 Te is passed into the NaOH solution to obtain the NaHTe precursor. Take a clean three-neck flask, add 2...

Embodiment 2

[0053] (1) Synthesis of gold nanoparticles

[0054] The preparation method of gold nanoparticles: take a clean Erlenmeyer flask, add 190mL ultrapure water, 10mL 0.2% chloroauric acid solution, stir the solution and heat it to boiling, and quickly add 3.6mL 1% citric acid three Sodium solution, after the solution turns from colorless to red, stop heating and continue stirring for 15 minutes to make gold nanoparticles.

[0055] (2) Synthesis of quantum dots

[0056] Take a clean Erlenmeyer flask, add 390mg Te powder, 189mg sodium borohydride, 4mL ultrapure water in turn, stir at room temperature for 5h until the black Te powder disappears completely, and white sodium borate crystals are produced. Under the protection of nitrogen, mix with H produced by the reaction of 0.5M dilute sulfuric acid 2 Te was passed into NaOH solution to obtain NaHTe precursor. Take a clean three-neck flask, add 2.6g CdCl successively 2 2.5H 2 O, 148mL water, 0.5mL 98% 3-mercaptopropionic acid, de...

Embodiment 3

[0065] (1) Synthesis of gold nanoparticles

[0066] The preparation method of gold nanoparticles: take a clean Erlenmeyer flask, add 190mL ultrapure water, 10mL 0.2% chloroauric acid solution, stir the solution and heat it to boiling, and quickly add 3.6mL 1% citric acid three Sodium solution, after the solution turns from colorless to red, stop heating and continue stirring for 15 minutes to make gold nanoparticles.

[0067] (2) Synthesis of quantum dots

[0068] Take a clean Erlenmeyer flask, add 390mg Te powder, 189mg sodium borohydride in sequence, then add 4mL ultrapure water, stir at room temperature for 5h until the black Te powder disappears completely, and white sodium borate crystals are produced, under nitrogen protection , will react with 0.5M dilute sulfuric acid to generate H 2 Te was passed into NaOH solution to obtain 0.15M NaHTe. Take a clean three-neck flask, add CdCl successively 2 2.5H 2 O, 148mL water, 0.5mL 3-mercaptopropionic acid, nitrogen deoxidat...

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Abstract

The invention belongs to the field of analytical chemistry, and relates to a method for detecting bisphenol A based on a quantum dot-gold nanoparticle self-assembled superstructure. The method comprises the following steps: at first, respectively preparing a fluorescent quantum dot and a gold-nanopartcle, preparing the quantum dot-gold controlled nanoparticle self-assembled superstructure by modifying a DNA on the surface of a nano materia and taking a DNA molecule as a template. The quantum dot is enabled to be separated from an assembly by using the specific recognition of bisphenol A and an aptamer modified on the surface of the quantum dot; the concentration of bisphenol A is detected by the change of fluorescence intensity in a system, the detection line of bisphenol A is 1.64 pg / mL, and the linearity range is 1-500 pg / mL. Quick and highly sensitive detection of bisphenol A can be achieved specifically, and an effective method is provided for detecting traces of noxious substances in food packaging materials.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to a method for detecting bisphenol A based on a quantum dot-gold nanometer assembly superstructure. Background technique [0002] Bisphenol A (Bisphenol A, referred to as BPA), chemical name 2,2-bis(4-hydroxyphenyl)propane, is a plasticizer synthesized by polycarbonate, epoxy resin and polyvinyl chloride resin, and has been widely used In hundreds of daily necessities such as canned food, beverage packaging, baby bottles, and water bottles. Although bisphenol A is a chemical with low toxicity, because its chemical structure is similar to estradiol and ethylene estradiol, like other environmental hormones, BPA can imitate or interfere with endogenous estrogen, even at very low doses. Animals have effects such as female precocious puberty, decreased sperm count, and benign prostatic hyperplasia. In addition, some data show that bisphenol A has certain embryotoxicity and teratogenic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 严文静章建浩杨龙平黄明明
Owner NANJING AGRICULTURAL UNIVERSITY
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