Nanograde biocomposite and applications thereof

A nano-scale, complex technology, applied in the biological field, can solve the problems of low sensor production reproducibility, irreversible sensor signal, weak storage stability, etc., and achieve the effects of easy detection, high production reproducibility, and high biocompatibility.

Active Publication Date: 2014-04-16
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these glucose sensing systems have some inherent deficiencies, such as: low reproducibility of sensor fabrication, low biocompatibility, weak storage stability, and irreversible sensor signal.

Method used

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  • Nanograde biocomposite and applications thereof
  • Nanograde biocomposite and applications thereof
  • Nanograde biocomposite and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Synthesis of nanoscale biocomplexes:

[0030] 1) Synthesis of graphene oxide-low molecular weight chitosan composite:

[0031] Mix 2 g of flake graphite with 1.5 ml of pure sulfuric acid, 0.8 g of potassium persulfate and 0.5 g of phosphorus pentoxide, and incubate at 80°C for 6 hours after mixing to obtain pre-oxidized graphite.

[0032] The pre-oxidized graphite powder was washed with distilled water until neutral, filtered and dried in air overnight at room temperature. Take 1 g of pre-oxidized graphite powder and place it in 29 ml of pure sulfuric acid at 0°C. Then keep the temperature below 20°C, gradually add 3 grams of potassium permanganate while stirring, then stir the mixture below 35°C for 2 hours, add 46 ml of distilled water after stirring, and continue stirring for 15 minutes. Finally, 140 ml of distilled water was added to terminate the reaction, followed by the addition of 2 ml of 30% hydrogen peroxide to turn the color of the mixture into bright yello...

Embodiment 2

[0042] Synthesis of Graphene Oxide-Low Molecular Weight Chitosan Composite

[0043] 1) 3g of flake graphite will be mixed with 2.0ml of pure sulfuric acid, 0.8g of potassium persulfate, and 0.8g of phosphorus pentoxide, and then incubated at 80 degrees for 7 hours to pre-oxidize the graphite. Then the product was washed with distilled water to neutrality, filtered and dried in air overnight at room temperature. 2g of pre-oxidized graphite powder was placed in 30ml of pure sulfuric acid at 0°C. Keep the temperature below 20 degrees, gradually add 5 grams of potassium permanganate while stirring, then stir the mixture below 35 degrees for 3 hours, then add 60 ml of distilled water, and continue to stir for 25 minutes. Then 200 ml of distilled water was added to stop the reaction, followed by 5 ml of 30% hydrogen peroxide to turn the color of the mixture into bright yellow. The mixture was centrifuged and washed with 300 mL of 10% HCl solution to remove residual metal ions. The...

Embodiment 3

[0047] The difference from Example 1 is:

[0048] Mix 8 g of flake graphite with 8 ml of pure sulfuric acid, 8 g of potassium persulfate and 5 g of phosphorus pentoxide, and incubate at 80°C for 10 hours after mixing to obtain pre-oxidized graphite. The pre-oxidized graphite powder was washed with distilled water until neutral, filtered and dried in air overnight at room temperature. Take 1g of pre-oxidized graphite powder and place it in 90ml of pure sulfuric acid at 0°C. Then keep the temperature below 20°C, gradually add 30 grams of potassium permanganate while stirring, then stir the mixture below 35°C for 5 hours, add 460 ml of distilled water after stirring, and continue stirring for 15 minutes. Finally, 400 ml of distilled water was added to terminate the reaction, followed by the addition of 20 ml of 30% hydrogen peroxide to turn the color of the mixture into bright yellow. Centrifuge the above-mentioned mixture that turns bright yellow, and wash the precipitate with...

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Abstract

The invention belongs to the field of biotechnology, and specifically relates to a nanograde biocomposite and applications thereof. The nanograde biocomposite is prepared by mixing a nanograde oxidized grapheme-low molecular weight chitosan compound solution and a recombinant allophycocyanin solution with PBS solution, wherein the concentration of the nanograde oxidized grapheme-low molecular weight chitosan compound solution ranges from 0.02 to 10<mu>g/ml, and the concentration of the recombinant allophycocyanin solution ranges from 0.001 to 10mg/ml. The nanograde biocomposite can be uses as biosensors, is high in specificity and sensitivity, and is nanoscaled; and application range of the biocomposite is wide.

Description

technical field [0001] The invention belongs to biotechnology, and in particular relates to a nanoscale biological compound and its application. Background technique [0002] Glucose, as the main carbon source and energy source, plays an important role in various aspects related to our daily life. Lack of glucose can inhibit the growth and division of cells, or limit the yield of products in food industry bioprocesses. However, excess glucose can also induce lactic acid formation through the glycolytic pathway. Therefore, monitoring and controlling blood glucose levels is not only necessary for healthy cell growth, but also serves as a clinical indicator of diabetes, and is of great importance in monitoring the maximum yield of biological processes in industry. Finding the "ideal" glucose sensor has been a long-term goal of many researchers, and a large number of glucose sensors have been developed. However, these glucose sensing systems have some inherent deficiencies, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/66A61B5/1455
Inventor 江婷婷蒲洋秦松崔玉琳王寅初
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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