8 results about "Allophycocyanin" patented technology

The invention provides a cold resistance comprehensive evaluation method for porphyra yezoensis, and belongs to the technical field of biological breeding. Four phenotypic indexes related to the cold resistance character are obtained through screening experiments, the four phenotypic indexes are the leaf area of the porphyra yezoensis thallus, the maximum light quantum efficiency Fv / Fm, the photosynthetic pigment chlorophyll a and allophycocyanin respectively, and the four phenotypic indexes can not only meet sustainable determination in a lossless state, but also can be used for detecting the cold resistance character of the porphyra yezoensis. And the cold resistance evaluation model constructed based on the few phenotypic indexes can achieve the purpose of accurately, efficiently and comprehensively evaluating the cold resistance of the porphyra yezoensis. Experimental results show that the method provided by the invention is relatively consistent with the cold resistance of an actual porphyra yezoensis variety or strain, the cold resistance grade of a to-be-detected sample can be accurately identified, the blank of cold resistance evaluation of the porphyra yezoensis is filled, and a technical basis is provided for subsequent screening, identification and breeding of a cold-resistant porphyra yezoensis culture variety.

The invention belongs to the technical field of bioactive peptides, and particularly relates to a phycocyanin peptide and application thereof in preparation of a product with functions of inhibiting PTGS2 expression and regulating an Akt / GSK3beta signal channel. According to the invention, phycocyanin is taken as a raw material, active polypeptides with specific sequences are obtained through an enzymolysis separation technology, and the amino acid sequences of the active polypeptides are respectively Leuu-Ala-Ser and Leuu-Gly-Leu. Experiments prove that the phycocyanin peptide prepared by the invention can significantly inhibit the expression of PTGS2, and can play a related regulation role by activating an Akt / GSK3beta signal channel, so that the technical blank of plant active peptide targeting PTGS2 and Akt / GSK3beta signal channels is filled, and the phycocyanin peptide can be widely applied to the development of related products for regulating the abnormal expression of PTGS2 and Akt / GSK3beta signal channels.

Disclosed herein is a method for enhancing immune response of a vaccine. The method includes preparing a Phycocyanin compound. The Phycocyanin compound includes at least one of C-phycocyanin (C-PC), Phycoerythrin (PE), Allophycocyanin (APC), phycocyanobilin (PCB), and combinations thereof. The method further includes administering the prepared Phycocyanin along with a vaccine composition to a living body.

This invention discloses a method for preparing phycocyanin, relating to the field of separation and purification technology. The method employs ammonium sulfate fractional crystallization for purification. The crystallization step utilizes precise control of the ammonium sulfate concentration and crystallization stirring time to optimize product purity and recovery rate. This method is simple, low-cost, easily scaled up, and can effectively purify phycocyanin. 620 / A 280 Purify food-grade raw materials of grades 1-3 to A 620 / A 280 It meets reagent-grade standards greater than 4.0 and effectively removes allophycocyanin. The crystallization process results in minimal loss of the target protein, a high overall recovery rate, and produces stable phycocyanin crystals suitable for industrial production, as well as long-term storage and transportation.

The present invention discloses a reagent composition for identifying mouse fibroblast subpopulations and a method for using the same, relating to the field of cell technology. The reagent composition includes an antigen blocking solution, an allophycocyanin (APC)-labeled Ly6C antibody, a phycoerythrin (PE)-labeled CyclinD1 antibody, a fluorescein isothiocyanate (FITC)-labeled DCN antibody, a PBS phosphate buffer, a cell digestion solution, and an erythrocyte lysis solution. The present invention can identify and sort DCN-positive fibroblast subpopulations in the lung tissue of mice with acute lung injury, or identify and sort DCN-positive fibroblast subpopulations in the lung tissue of mice with acute lung injury that are undergoing tissue damage or repair, providing conditions for in-depth research on the role and mechanism of DCN-positive fibroblasts in ARDS, and exploring clinical treatment or prognostic targets.

The present invention relates to the field of immunology technology, and more particularly to a fluorescent protein tandem dye and a method for labeling a conjugated monoclonal antibody therewith, as well as a kit. The tandem dye is composed of two fluorescent molecules, with a macromolecular protein dye APC serving as a donor and a small molecule organic dye serving as an acceptor, the two being covalently bonded. After the tandem dye is combined, the donor dye APC can be excited by a laser emitted by an exciter, while the small molecule acceptor dye cannot. The energy emitted by the donor dye APC after being excited is transferred to the acceptor dye, causing the acceptor dye to excite light of a specific wavelength. The tandem dye has the excitation spectrum of the donor dye APC and the emission spectrum of the small molecule acceptor dye, becoming a unique fluorescent dye that is completely different from the donor dye APC and the acceptor dye and has a relatively large Stokes shift.

Disclosed herein are edible compositions containing a blue colorant having a phycocyanin content of at least 35 wt.% or at least 40 wt.% and uses thereof. The blue colorant can be derived from a natural source, such as Spirulina ( Spirulina ) or Galdieria sulphuraria ( Galdieria sulphuraria ), and can provide color characteristics similar to those provided by the synthetic blue colorant FD&C Blue No. 1.

The invention provides a preparation method of an allophycocyanin labeled antibody, which is characterized in that after reaction steps are adjusted, an APC-cross-linking agent intermediate product formed by coupling APC and a cross-linking agent does not need to be sealed, and the APC-cross-linking agent intermediate product can directly react with the sulfydryl on the antibody, so that the APC-cross-linking agent is coupled with the antibody without using other cross-linking agents; therefore, the preparation efficiency is higher.