Silicon core quantum dot shell composite nano material, preparation method, application and product

A technology of composite nanomaterials and quantum dots, which is applied in the field of silicon core quantum dot shell composite nanomaterials and preparation, can solve the problems of limiting the application in the field of immunochromatography, difficult to recover by centrifugation, etc., and achieve superior performance, convenient storage, and dispersibility Good results

Active Publication Date: 2020-05-08
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the small size effect of quantum dots used as fluorescent labels, it is difficult to recover by centrifugation during the preparation process and antibody modification process, which limits its application in the field of immunochromatography

Method used

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  • Silicon core quantum dot shell composite nano material, preparation method, application and product
  • Silicon core quantum dot shell composite nano material, preparation method, application and product
  • Silicon core quantum dot shell composite nano material, preparation method, application and product

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preparation example Construction

[0076] The present invention also includes the preparation method of the above-mentioned composite nanomaterial, comprising the following steps: 2 The electropositive polymer is modified on the surface of the nanoparticle, and then the carboxylated quantum dot is modified to obtain a composite nanomaterial.

[0077] Specifically, SiO 2 The nanoparticles are negatively charged, and the positively charged polymers with positive charge act on SiO 2 Surface self-assembly of nanoparticles to form SiO surface-modified with electropositive polymers 2 Nanoparticles, and then use the charge to decorate the negatively charged quantum dots on the surface of the positively charged polymer to obtain a composite nanomaterial. The invention utilizes the self-assembly property of the electropositive polymer and adopts a layer-by-layer self-assembly method to prepare composite nanomaterials. The preparation method is simple, mature and repeatable, and can realize large-scale production.

[...

Embodiment 1

[0088] A silicon core quantum dot shell composite nanomaterial prepared by the present invention, including SiO 2 Quantum dot shell composed of core, PEI interlayer and carboxylated quantum dots modified with mercaptopropionic acid (MPA), SiO 2 The particle size of the particles is 150nm, the thickness of the PEI self-assembled layer is 5nm, the particle size of the adsorbed carboxylated quantum dots (CdSe / ZnS-COOH) is 10nm, and the final particle size of the silicon ball quantum dots is about 180nm, which has good dispersion and fluorescence properties.

[0089] A preparation method of the silicon core quantum dot shell composite nanomaterial of the above-mentioned present embodiment, such as figure 1 shown, including the following steps:

[0090] (1) Preparation of 150nm SiO with good dispersion 2 core:

[0091] Add 100mL of absolute ethanol, 6mL of deionized water, and 28% ammonia water into a 200mL jar in turn, add a clean stir bar, and stir magnetically for 20min. St...

Embodiment 2

[0099] The outermost layer of the silicon core quantum dot-shell composite nanomaterial in Example 1 is a carboxylated quantum dot structure modified by mercaptopropionic acid (MPA), and the surface has a large number of free carboxyl groups that can be used for antibody coupling, and can realize nano Surface functionalization of materials. The steps of antibody modification on the surface of the silicon core quantum dot shell composite nanomaterial of the present invention are as follows: Figure 5 shown, including the following steps:

[0100] 1mg SiO 2 @QDs powder was dissolved in 1 mL of 2-(N-morpholine)ethanesulfonic acid solution (0.1M, pH5.5), then 100 μL of carbodiimide solution (0.01M) and 20 μL of N-hydroxysuccinimide solution were added (0.1M), ultrasonic reaction for 15min to activate SiO 2 Carboxyl groups on the surface of @QDs; SiO was then recovered by centrifugation 2 @QDs, resuspend in 200 μL PBST solution (0.01M, pH7.4); add 15 μg antibody, shake at room ...

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Abstract

The invention relates to the field of nano materials and novel quantum dots, and particularly provides a silicon core quantum dot shell composite nano material, a preparation method, application and aproduct. The composite nano material provided by the invention has a core-shell structure, an inner core layer is SiO2 nano particles, an outer shell layer is carboxylated quantum dots, and an electropositive polymer layer is also arranged between the inner core layer and the outer shell layer. The preparation method comprises the following steps: firstly, modifying the surfaces of SiO2 nanoparticles with an electropositive polymer, and then adsorbing a layer of carboxylated quantum dots to form the composite nano material with the silicon core quantum dot shell. The composite nano material is good in dispersity, adjustable in particle size, high in fluorescence intensity and easy to prepare in batches, and can be used for capturing and high-sensitivity detection of target substances in complex samples.

Description

technical field [0001] The invention relates to the field of nanomaterials and novel quantum dots, in particular to a silicon core quantum dot shell composite nanomaterial, a preparation method, an application and a product. Background technique [0002] As a representative substance in the nanometer field, quantum dots have optical and chemical properties such as narrow emission spectrum, wide excitation spectrum, controllable emission wavelength, strong fluorescence, long fluorescence lifetime, and size dependence. In recent years, they have been widely used in many fields of biology. Applications. Since the 20th century, quantum dots have been widely used in immunochromatographic detection systems as a new type of fluorescent label. These quantum dot-based fluorescent immunochromatography have achieved simultaneous and rapid detection of multi-component biochemical molecules. Quantum dot labels generate photostable, single-wavelength excited, readable fluorescent signals...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/569G01N33/58C01B33/18B82Y30/00B82Y40/00
CPCG01N33/588G01N33/587G01N33/558G01N33/56916C01B33/186B82Y30/00B82Y40/00G01N2333/255G01N2469/10Y02A50/30
Inventor 王升启肖瑞汪崇文杨兴胜
Owner ACADEMY OF MILITARY MEDICAL SCI
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