Cardiopulmonary quintuplet detection kit , rare earth nano fluorescence detection card thereof and detection method thereof

Abstract

The invention discloses a cardiopulmonary quintuplet detection kit , a rare earth nano fluorescence detection card thereof and a detection method thereof. The content of the cTnI/CKMB/Myo/D-Dimer/NT-proBNP in human blood can be accurately and quantitatively detected; a rare earth nano fluorescent microsphere detection technology is used; fluorescence interference of a sample can be avoided; the fluorescent microsphere is a rare earth fluoride near-infrared two-region light-emitting nano material and has a low background; the microsphere is connected with the antibody by virtue of a covalent bond, so that a labeled product is stable, has the characteristics of wide detection range, high sensitivity, high accuracy, rapidness, simplicity and convenience in detection and the like, and can helpdoctors to accurately diagnose the cardio-pulmonary function in the early stage.

Description

technical field [0001] The invention relates to a heart-lung five-unit detection kit, a rare earth nanometer fluorescence detection card and a detection method thereof. Background technique [0002] Troponin is composed of three subunits of troponin I, T, and C, and together with tropomyosin, it regulates the interaction between actin and myosin by regulating the activity of Ca2+ on striated actin ATPase. After myocardial injury, the cardiac troponin complex is released into the blood, and after 4-6 hours, it begins to rise in the blood, and the elevated troponin I can remain in the blood for 6-10 days, providing a longer detection period. Cardiac troponin I (cTnI) has a high degree of myocardial specificity and sensitivity, so it has become an ideal marker of myocardial infarction. [0003] Myoglobin (Myo) is a binding protein composed of a peptide chain and a heme prosthetic group, and is a protein that stores oxygen in muscles. It can rise as soon as 2 hours after the ...

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Problems solved by technology

[0008] In the prior art, cardiac markers are only tested for a single index and the three myocardial indicators cTnI, CK-MB, and Myo, and the joint detection of the five cardiopulmonary items is less. In addition, the detection methods mainly include immunoturbidimetry and fluorescence immunochromatography. method, colloidal gold method, etc.

Among them, the colloidal gold method has the advantages of simple and fast operation, but low sensitivity and inaccurate quantification; immunoturbidimetric method is more sensitive and accurate, and can be applied to automatic biochemical analyzers, but requires instruments and takes a long time, and is suitable for processing a large number of samples. It cannot meet the purpose of rapid detection; immunofluorescence method is to covalently bind cTnI / CKMB / Myo / D-Dimer / NT-proBNP antibody to the surface active group of fluorescent microspheres, and judge the result by whether the detection line produces fluorescence after excitation , fast and convenient, accurate quantification and high sensitivity, stable markers, etc., widely used in the field of medical immunoassay

[0009] However, many complexes and proteins in biological fluids and serum can fluoresce by themselves, and the spectral range of commonly used fluorescent markers is mostly in the visible light region (400-750nm), and the use of fluorescent markers in the visible region will produce a strong background Fluorescence, which interferes with the detection signal and reduces detection sensitivity

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