The present invention provides a test paper card which is used for simultaneously detecting a plurality of
colloidal gold immunochromatographic assays, and relates to the immuno-chromatographic technology of the detecting test strip, in particular to a
colloidal gold immunochromatographic
assay detection device which can be used for qualitatively and simultaneously detecting a plurality of antigens or antibodies. The device consists of a plastic card box and two
test strips; the plastic card box has a positive side and a negative side, a sampling window and an observing window are positioned on the positive side, and an observing window is arranged on the negative side; a sample pad, a
glass fiber membrane, a chromatographic membrane and an absorbent pad are arranged on the test strip in sequence and are used for absorbing the antigens or antibodies on the specific regions of the chromatographic membrane, so as to form a
quality control line and one or more than one detection line(s); the two
test strips are separated by a plastic bottom lining, and no plastic bottom lining is arranged in the position of the sample pad, so the sample pad is connected with the two
test strips; the sample pad corresponds to the sampling window, and the chromatographic membranes on the two test strips respectively correspond to the observations on the positive side and on the negative side; a
colloidal gold labeling material is pre-buried in the position of
glass fiber membrane; the sample is added into the sample pad, the test strips of the positive side and the negative side simultaneously perform the function of capillary proliferation towards the absorbent pad, and after the immunoreaction, the
immune complex which can be seen by naked eyes is formed.