Carbon quantum dot fluorescence labeling material with egg white used as carbon source as well as preparation method and application of carbon quantum dot fluorescence labeling material

A technology of fluorescent labeling and carbon quantum dots, applied in luminescent materials, chemical instruments and methods, etc., can solve the problems of high demand and high cost, and achieve the effects of simple equipment, low cost and short reaction time

Active Publication Date: 2015-04-29
北京中农探味科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method requires expensive equipment such as low-temperature plasma, which is expensive

Method used

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  • Carbon quantum dot fluorescence labeling material with egg white used as carbon source as well as preparation method and application of carbon quantum dot fluorescence labeling material
  • Carbon quantum dot fluorescence labeling material with egg white used as carbon source as well as preparation method and application of carbon quantum dot fluorescence labeling material

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Embodiment 1

[0040] A method for preparing a carbon quantum dot fluorescent labeling material using egg white as a carbon source, comprising the following steps:

[0041] (1) Take an egg and break it, keep only the egg white part, absorb 4ml of egg white and dissolve it in 40ml of deionized water, homogenize for about 3 minutes;

[0042] (2) Take 10mL of the mixed solution in step (1) and transfer it to a 50ml small beaker, add concentrated H 2 SO 4 4mL of the solution, stirred evenly, and placed in a water bath at 50°C for 2 hours;

[0043] (3) Add NaOH solution, adjust the pH to 7, and then centrifuge at a speed of 5000r / min for 5min to remove undissolved residues;

[0044] (4) Add 3mL of dichloromethane to the supernatant of step (3), then centrifuge at a speed of 5000r / min for 5min to remove non-fluorescent fat-soluble substances;

[0045] (5) Pass the supernatant in step (4) through a 0.45 μm filter membrane to prepare a fluorescent labeling material.

[0046] From figure 1 It ca...

Embodiment 2

[0048]A method for preparing a carbon quantum dot fluorescent labeling material using egg white as a carbon source, comprising the following steps:

[0049] (1) Take an egg and break it, keep only the egg white part, absorb 4ml of egg white and dissolve it in 20ml of deionized water, homogenize for about 5 minutes;

[0050] (2) Take 10mL of the mixed solution in step (1) and transfer it to a 50ml small beaker, add concentrated H 2 SO 4 Solution 4mL, stir evenly, and place in water bath at 60°C for 3 hours;

[0051] (3) Add NaOH solution, adjust the pH to 7.5, and then centrifuge at a speed of 5500r / min for 5min to remove undissolved residues;

[0052] (4) Add 1mL of dichloromethane to the supernatant of step (3), then centrifuge at a speed of 5500r / min for 5min to remove non-fluorescent fat-soluble substances;

[0053] (5) Pass the supernatant in step (4) through a 0.1 μm filter membrane to prepare a fluorescent labeling material.

Embodiment 3

[0055] A method for preparing a carbon quantum dot fluorescent labeling material using egg white as a carbon source, comprising the following steps:

[0056] (1) Take an egg and break it, and only keep the egg white part, absorb 4ml of egg white and dissolve it in 80ml of deionized water, homogenize for about 2 minutes;

[0057] (2) Take 10mL of the mixed solution in step (1) and transfer it to a 50ml small beaker, add concentrated H 2 SO 4 4mL of the solution, stirred evenly, and placed in a water bath at 40°C for 4 hours;

[0058] (3) Add NaOH solution, adjust the pH to 7, and then centrifuge at a speed of 6000r / min for 5min to remove undissolved residues;

[0059] (4) Add 5 mL of dichloromethane to the supernatant of step (3), then centrifuge at 6000 r / min for 5 min to remove non-fluorescent fat-soluble substances;

[0060] (5) Pass the supernatant in step (4) through a 0.22 μm filter membrane to prepare a fluorescent labeling material.

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Abstract

The invention relates to a carbon quantum dot fluorescence labeling material with egg white used as a carbon source as well as a preparation method and an application of the carbon quantum dot fluorescence labeling material. The egg white is used as a raw material, and a simple method for one-step synthesis of a high-fluorescence-quantum-efficiency carbon quantum dot is established. The method comprises steps as follows: the egg white is mixed with water; then a mixed liquid is mixed with concentrated sulfuric acid, and a mixture is placed in a water bath; the pH is adjusted, and undissolved residues are removed in a centrifugal manner; then dichloromethane is added to a centrifuged supernate, and a non-fluorescence fat-soluble substance is removed in the centrifugal manner; finally, the centrifuged supernate passes through a film, and the fluorescence labeling material is prepared. According to the method, high-temperature and high-pressure reactions are not required to be performed by equipment such as plasma emitters, hydrothermal reaction kettles, muffle furnaces and the like during synthesis, only conventional instruments and equipment such as the water bath and the like are required, reaction conditions are mild, operations are simple and convenient, the cost is very low, the method can be implemented in ordinary laboratories, and the method has the good generalizability.

Description

technical field [0001] The invention relates to the technical field of material synthesis, in particular to a carbon quantum dot fluorescent labeling material using egg white as a carbon source and a preparation method and application thereof. Background technique [0002] Carbon quantum dots are a new type of nanomaterial with carbon as the skeleton discovered in 2004. It is a kind of dispersed, spherical-like nanoscale subject with a size less than 100nm. Compared with traditional semiconductor quantum dots, rare earth fluorescent labeling materials and organic fluorescent dyes, it has the advantages of low toxicity, good biocompatibility, etc., and has the advantages of adjustable luminous range, good photostability, no light flicker, and easy functionalization. , has great application prospects in the fields of cell imaging and biochemical analysis, and has developed rapidly in recent years. [0003] The carbon source of carbon quantum dots has gradually changed from ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/02C09K11/65
Inventor 卢嘉王淼陈卫华王凤忠黄亚涛范蓓孙玉凤林菲夏双梅单吉浩
Owner 北京中农探味科技有限公司
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