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Near-infrared luminescence bio-fluorescence labeling material and preparation method

A technology of luminescent biology and fluorescent labeling, which is applied in the direction of luminescent materials, chemical instruments and methods, etc., can solve the problems of narrow emission peaks, weakened optical properties, high research and production costs, and achieve excellent water resistance, good chemical stability, and preparation low cost effect

Inactive Publication Date: 2013-04-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Compared with traditional organic fluorescent dyes, quantum dots have wide excitation, narrow and symmetrical emission peaks, and the emission wavelength can be adjusted by controlling its size and composition. The fluorescence intensity and stability are about 100 times that of ordinary fluorescent dyes. There is almost no photobleaching phenomenon and good biocompatibility in biological systems. These characteristics enable them to overcome the problems that organic fluorophores are difficult to solve as probes. However, quantum dots generally have biological toxicity. Although the coating method can Weaken the toxicity, but also weaken its optical performance, so the application of quantum dots in biological and other fields is still being researched and explored
Compared with organic dyes and quantum dot materials, rare earth-doped luminescent materials have low toxicity, but as the price of rare earths increases, their research and production costs are also increasing

Method used

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  • Near-infrared luminescence bio-fluorescence labeling material and preparation method
  • Near-infrared luminescence bio-fluorescence labeling material and preparation method
  • Near-infrared luminescence bio-fluorescence labeling material and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Select barium carbonate, ammonium phosphate and manganese trioxide as starting raw materials, according to Ba 3 [P (1-x) mn x o 4 ] 2 (x=0.2, 0.5, 1, 1.5, 2.0, 2.5mol%) shown in the molar ratio, respectively accurately weigh the three raw materials, control the total weight of the mixture to about 50 grams. After 50 grams of the mixture were mixed by ball milling, they were put into a corundum crucible, and then the crucible was put into a high-temperature electric furnace. Precisely control the heating rate, control the decomposition reaction rate of the ammonium sulfate compound, prevent the mixture from overflowing from the crucible, and pre-fire the sample at 400°C for 5 hours. The pre-fired sample was taken out, ground and mixed again, put into a crucible, and fired at 1000° C. for 14 hours to obtain a pentavalent manganese ion-doped barium phosphate near-infrared luminescent material. Disperse the prepared near-infrared light-emitting phosphor powder in ethan...

Embodiment 2

[0036] Choose barium nitrate, diammonium hydrogen phosphate and manganese nitrate as starting raw materials, according to Ba 3 [P (1-x) mn x o4 ] 2 (x=0.2, 0.5, 1, 1.5, 2.0, 2.5 mol%) in the molar ratio, weigh the three raw materials respectively, and control the total weight of the mixture to about 50 grams. After 50 grams of the mixture were mixed by ball milling, they were put into a corundum crucible, and then the crucible was put into a high-temperature electric furnace. Precisely control the heating rate, control the decomposition reaction rate of the ammonium sulfate compound, prevent the mixture from overflowing from the crucible, and pre-fire the sample at 400°C for 5 hours. The pre-fired sample was taken out, ground and mixed again, put into a crucible, and fired at 1000° C. for 14 hours to obtain a pentavalent manganese ion-doped barium phosphate near-infrared emitting phosphor. Disperse the prepared near-infrared light-emitting phosphor powder in ethanol, and u...

Embodiment 3

[0038] Select barium chloride, ammonium dihydrogen phosphate and manganese chloride as starting materials, according to Ba 3 [P (1-x) mn x o 4 ] 2 (x=0.2, 0.5, 1, 1.5, 2.0, 2.5 mol%) in the molar ratio, weigh the three raw materials respectively, and control the total weight of the mixture to about 50 grams. After 50 grams of the mixture were mixed by ball milling, they were put into a corundum crucible, and then the crucible was put into a high-temperature electric furnace. Precisely control the heating rate, control the decomposition reaction rate of the ammonium sulfate compound, prevent the mixture from overflowing from the crucible, and pre-fire the sample at 400°C for 5 hours. The pre-fired sample was taken out, ground and mixed again, put into a crucible, and fired at 1000° C. for 14 hours to obtain a pentavalent manganese-doped barium phosphate near-infrared emitting phosphor. Using modern femtosecond laser technology, laser pulverization is carried out at a power...

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Abstract

The invention discloses a near-infrared luminescence bio-fluorescence labeling material. The crystal structure of the material is a rhombohedron structure and the molecular formula is Ba3[P(1-x)MnxO4]2, wherein 0.002 <= x <= 0.025. The invention also discloses a preparation method of the material, comprising the steps of: (1) weighing the compound raw materials containing barium, phosphorus, and manganese according to a molar ratio of elements; (2) preburning for 3-8 hours at the temperature of 300-500 DEG C after grinding and mixing; and (3) taking out the sample after preburning, regrounding and mixing, and firing for 10-20 hours at a temperature of 900-1100 DEG C to obtain a near-infrared luminescence fluorescent powder. The bio-fluorescence labeling material of the invention has the advantages of strong luminescence, good chemical stability, excellent water resistance, broad excitation, narrow emission peak, long life and good biocompatibility.

Description

technical field [0001] The invention relates to a near-infrared luminescent material, in particular to a near-infrared luminescent bioluminescence marking material and a preparation method. Background technique [0002] Due to its high sensitivity, high selectivity, multiple characteristic parameters and wide dynamic range, fluorescent probe technology plays an important role in research work in many disciplines. With the continuous development of fluorescent probe synthesis technology, its application is becoming more and more extensive. [0003] Traditional organic fluorophores mainly include: fluorescein dyes, rhodamine dyes, cyanine dyes, and other fluorescent dyes. These organic dyes have narrow absorption spectra, broad and overlapping emission spectra, poor water solubility, easy to decompose, difficult to couple with biomolecules, and poor stability; in addition, the complexity of biological systems often requires simultaneous observation of several groups. If you ...

Claims

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Application Information

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IPC IPC(8): C09K11/71
Inventor 曹人平邱建荣张芳腾马志军廖臣兴
Owner SOUTH CHINA UNIV OF TECH
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