2results about How to "Fast magnetic response" patented technology

The application discloses a kind of functionalized magnetic beads, which are magnetic microspheres formed by wrapping magnetic beads with bifunctional nucleic acid, and can specifically bind mRNA.A fungal mRNA extraction kit composed of functionalized magnetic beads includes grinding material, lysing solution I, lysing solution II, magnetic bead binding solution, washing solution I, washing solution II and eluent.A mRNA extraction method includes sequentially adding grinding material, lysing solution I, lysing solution II, magnetic bead binding solution, washing solution I, washing solution II and eluent after sample pretreatment to obtain mRNA product.The application breaks through the traditional mRNA extraction method, obtains high-quality mRNA, and has the advantages of simple operation, safety, high sensitivity, good reproducibility and low cost.The mRNA obtained by the application can provide high-quality templates for RT-PCR detection, improve the specificity and sensitivity of detection, and has wide application prospects in the fields of clinical diagnosis of diseases, judicial identification, food safety detection, microbial detection and molecular biology.

The application discloses a preparation method of amino magnetic beads, which comprises the following steps: coating a magnetic nanoparticle with a silicon shell to form a silicon magnetic bead; modifying the silicon magnetic bead to form a modified silicon magnetic bead; reacting the modified silicon magnetic bead with an organic amine to form an aminated silicon magnetic bead; and performing a polymerization reaction on the aminated silicon magnetic bead to form the amino magnetic bead. The application forms a dense polyamide polymer layer on the surface of the magnetic bead core through an amidation coating scheme, so as to optimize the structure of the polymer layer of the magnetic bead. The magnetic response speed of the prepared magnetic bead is relatively fast due to the relatively large proportion of the magnetic core part of the magnetic bead and the relatively large number of hydrophilic active groups and charges on the surface of the polymer layer. The prepared magnetic bead has excellent later modification extensibility due to the adjustable type of the active groups carried on the surface.