Colloidal gold immune chromatography test card for simultaneously detecting multiple-item
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A technology of immunochromatographic test paper and colloidal gold, which is applied in the direction of measuring devices, analyzing materials, and analyzing materials by chemical reactions
Inactive Publication Date: 2009-02-04
范行良 +1
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[0002] As an immune analysis technique, immunochromatography is a common method for antigen, hapten and antibody detection. Colloidal gold detection test strip is the most common one. It has been described in many patents, and colloidal gold infiltration is also used. Filtration method for multiple simultaneous detection patent declarations, such as: "Colloidal gold immunochromatographic rabies virus antibody detection reagent and preparation method" application number: 00110462.4, "a reagent for detecting morphine" application number: 02137355.8, "for simultaneous Kit for det
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Embodiment 1
[0012] ToRCH series IgM antibody detection
[0013] 1. A certain amount of TO and RV antigens are respectively coated on one chromatographic membrane 5 by ribbon-shaped physical adsorption, and HSV and CMV antigens are respectively coated on another chromatographic membrane 5 by ribbon-shaped physical adsorption, and the chromatographic membrane 5 Block with 2% BSA.
[0014] 2. The chromatographic membrane 5 is assembled with a sample pad 2, a glass fiber membrane 3 and an absorbent pad 6 to form TO, RV antibody detection test strips and HSV, CMV antibody detection test strips.
[0015] 3. Anti-human IgM monoclonal antibody colloidal gold markers are pre-embedded on the glass fiber membrane 3 of the test strip respectively.
[0016] 4. TO, RV antibody detection test strips and HSV, CMV antibody detection test strips are adhered to both sides of the plastic substrate 7 respectively.
[0017] 5. Assemble the plastic test paper cartridge.
[0018] 6. Add the sample liquid into...
Embodiment 2
[0020] Influenza virus typing test
[0021] 1. A certain amount of anti-influenza virus IgG (H1N1 subtype, H3N2 subtype) is coated on a chromatographic membrane 5 by physical adsorption in a band shape. A certain amount of anti-influenza virus antibody IgG is coated on another chromatographic membrane 5 in the form of ribbon physical adsorption, and the chromatographic membrane 5 is blocked with 2% BSA.
[0022] 2. The chromatographic membrane 5 is assembled with a sample pad 2, a glass fiber membrane 3 and an absorbent pad 6 to form a type A influenza virus detection test strip and a type B influenza virus detection test strip.
[0023] 3. Anti-type A influenza virus antibody IgG (H1N1 subtype, H2N3 subtype) colloidal gold markers are pre-embedded on the glass fiber membrane of the type A influenza virus detection test strip. The anti-influenza virus antibody IgG colloidal gold marker is pre-embedded on the glass fiber membrane 3 of the influenza B virus detection test strip...
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Abstract
The present invention provides a test paper card which is used for simultaneously detecting a plurality of colloidal gold immunochromatographic assays, and relates to the immuno-chromatographic technology of the detecting test strip, in particular to a colloidal gold immunochromatographic assay detection device which can be used for qualitatively and simultaneously detecting a plurality of antigens or antibodies. The device consists of a plastic card box and two test strips; the plastic card box has a positive side and a negative side, a sampling window and an observing window are positioned on the positive side, and an observing window is arranged on the negative side; a sample pad, a glass fiber membrane, a chromatographic membrane and an absorbent pad are arranged on the test strip in sequence and are used for absorbing the antigens or antibodies on the specific regions of the chromatographic membrane, so as to form a quality control line and one or more than one detection line(s); the two test strips are separated by a plastic bottom lining, and no plastic bottom lining is arranged in the position of the sample pad, so the sample pad is connected with the two test strips; the sample pad corresponds to the sampling window, and the chromatographic membranes on the two test strips respectively correspond to the observations on the positive side and on the negative side; a colloidal gold labeling material is pre-buried in the position of glass fiber membrane; the sample is added into the sample pad, the test strips of the positive side and the negative side simultaneously perform the function of capillary proliferation towards the absorbent pad, and after the immunoreaction, the immune complex which can be seen by naked eyes is formed.
Description
technical field [0001] The invention relates to an immunological detection device, which is characterized in that it adopts the immunochromatographic technology of a test strip, and can simultaneously detect two or more antigens or antibodies by adding a sample liquid at one time. Background technique [0002] As an immune analysis technique, immunochromatography is a common method for antigen, hapten and antibody detection. Colloidal gold detection test strip is the most common one. It has been described in many patents, and colloidal gold infiltration is also used. Filtration method for multiple simultaneous detection patent declarations, such as: "Colloidal gold immunochromatographic rabies virus antibody detection reagent and preparation method" application number: 00110462.4, "a reagent for detecting morphine" application number: 02137355.8, "for simultaneous Kit for detecting multiple infectious diseases and its preparation method" application number: 01126500.0, "kit ...
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