Colloidal gold immune chromatography test card for simultaneously detecting multiple-item

Abstract

The present invention provides a test paper card which is used for simultaneously detecting a plurality of colloidal gold immunochromatographic assays, and relates to the immuno-chromatographic technology of the detecting test strip, in particular to a colloidal gold immunochromatographic assay detection device which can be used for qualitatively and simultaneously detecting a plurality of antigens or antibodies. The device consists of a plastic card box and two test strips; the plastic card box has a positive side and a negative side, a sampling window and an observing window are positioned on the positive side, and an observing window is arranged on the negative side; a sample pad, a glass fiber membrane, a chromatographic membrane and an absorbent pad are arranged on the test strip in sequence and are used for absorbing the antigens or antibodies on the specific regions of the chromatographic membrane, so as to form a quality control line and one or more than one detection line(s); the two test strips are separated by a plastic bottom lining, and no plastic bottom lining is arranged in the position of the sample pad, so the sample pad is connected with the two test strips; the sample pad corresponds to the sampling window, and the chromatographic membranes on the two test strips respectively correspond to the observations on the positive side and on the negative side; a colloidal gold labeling material is pre-buried in the position of glass fiber membrane; the sample is added into the sample pad, the test strips of the positive side and the negative side simultaneously perform the function of capillary proliferation towards the absorbent pad, and after the immunoreaction, the immune complex which can be seen by naked eyes is formed.

Description

technical field [0001] The invention relates to an immunological detection device, which is characterized in that it adopts the immunochromatographic technology of a test strip, and can simultaneously detect two or more antigens or antibodies by adding a sample liquid at one time. Background technique [0002] As an immune analysis technique, immunochromatography is a common method for antigen, hapten and antibody detection. Colloidal gold detection test strip is the most common one. It has been described in many patents, and colloidal gold infiltration is also used. Filtration method for multiple simultaneous detection patent declarations, such as: "Colloidal gold immunochromatographic rabies virus antibody detection reagent and preparation method" application number: 00110462.4, "a reagent for detecting morphine" application number: 02137355.8, "for simultaneous Kit for detecting multiple infectious diseases and its preparation method" application number: 01126500.0, "kit ...

AI Technical Summary

Problems solved by technology

[0002] As an immune analysis technique, immunochromatography is a common method for antigen, hapten and antibody detection. Colloidal gold detection test strip is the most common one. It has been described in many patents, and colloidal gold infiltration is also used. Filtration method for multiple simultaneous detection patent declarations, such as: "Colloidal gold immunochromatographic rabies virus antibody detection reagent and preparation method" application number: 00110462.4, "a reagent for detecting morphine" application number: 02137355.8, "for simultaneous Kit for detecting multiple infectious diseases and its preparation method" application number: 01126500.0, "kit for simultaneous detection of four teratogenic diseases and its preparation method" application number: 01126586.8, and now there are various similar products Detection kits are available on the market. This type of reagent uses colloidal gold as an indicator. It has the advantages of simple operation, rapidity and no need for instrument detection. It has been widely used in the initial screening of samples, but it can only be detected on a single test strip. one or two targets

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