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Immunoassay based on carbon nanomaterial

A technology of carbon nanomaterials and immunoassays, which is used in material inspection products, biological tests, etc., to avoid preparation and storage problems and achieve good optical stability.

Active Publication Date: 2012-09-12
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its excellent optical properties have not been used in immunoassays

Method used

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  • Immunoassay based on carbon nanomaterial
  • Immunoassay based on carbon nanomaterial
  • Immunoassay based on carbon nanomaterial

Examples

Experimental program
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Effect test

Embodiment 1

[0018] 1. Synthesis of fluorescent carbon nanomaterials

[0019] Synthesize fluorescent carbon nanomaterials by microwave heating: mix 1.0g PEG1500 and 15mL glycerin, heat it with microwave for 1 minute to form a transparent viscous liquid, then add 0.5g serine as carbon source, and heat the mixture for 10 minutes with microwave, between reagents Crosslink each other to form carbon dots; after removing impurities by dialysis, store the carbon dots in a refrigerator at 4°C, or store the carbon dots after rotary evaporation to dryness.

[0020] Synthesis of fluorescent carbon nanomaterials by hydrothermal method: Mix 1.0g PEG1500, 15mL glycerin and 0.5g serine in a reaction kettle, seal and heat at 250°C for 3 hours; Store in a 4°C refrigerator.

[0021] No matter the fluorescent carbon nanomaterials synthesized by microwave heating method or hydrothermal method, the carbon dots have the same properties; figure 1 It is the fluorescence spectrum diagram of carbon dots. From lef...

Embodiment 2

[0033] Competitive reaction mode immune response and its immunodetection:

[0034] In this embodiment, the samples to be tested are disease markers. Specifically, they are macromolecular disease markers such as tumor markers CA153, CEA, and AFP, or small molecular compounds such as triiodothyronine, estradiol, and estriol.

[0035] First, use a method similar to step 3 in Example 1 to bind anti-small molecule antibodies (such as anti-triiodothyronine antibodies) to magnetic particles; then use a method similar to step 2 in Example 1 to bind small molecule standards The substance triiodothyronine is labeled with carbon dots; then the antibody magnetic particle complex, the carbon dot-labeled triiodothyronine (immune carbon dots), and the sample solution to be tested are added to the reactor for competition reaction After 1 hour, immune complexes were formed, and the immune complexes were separated using a magnetic separator. The oxidizing reagent cerium sulfate or potassium p...

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Abstract

The invention provides an immunoassay based on a carbon nanomaterial (carbon spot), comprising the following steps of: step A, preparing a carbon spot, and marking an immunoreagent antigen or antibody on a surface of the carbon spot so that the carbon spot becomes an immunoreagent-marked carbon spot; step B, mixing the immunoreagent-marked carbon spot, a sample to be tested and a solid phase substance coated by the immunoreagent antigen or antibody to complete immunoreactions, thus forming an immune complex, and separating the immune complex from a free immunoreagent; and step C, adding an oxidant to the immune complex for chemiluminescence detection, and / or directly enabling the immune complex to be subjected to fluorescence detection. According the immunoassay based on the carbon nanomaterial, the chemiluminescence detection and the fluorescence detection can be performed by using the carbon nanomaterial, and the optical stability is good.

Description

technical field [0001] The invention belongs to the field of immune detection and analysis, in particular to an immune analysis method based on carbon nanomaterials (carbon dots). Background technique [0002] Because of its unique specificity, immunoassay is widely used in clinical diagnosis and environmental detection. Among the many detection methods of immunoassay, chemiluminescence detection method has been paid attention to because of its advantages of high sensitivity, wide linear range, simple instrument, rapid analysis and high degree of automation. Commonly used labels for chemiluminescent immunoassays can be divided into three categories. One, direct labeling with chemiluminescent substances. Such substances mainly include hydrazide compounds (such as luminol, isoluminol and their derivatives), acridinium esters and their derivatives, and imidazole compounds. The chemiluminescent reaction of luminol and its derivatives must be observed in the presence of an app...

Claims

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Application Information

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IPC IPC(8): G01N33/53
Inventor 林金明林珍周云张炜奋
Owner TSINGHUA UNIV
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