80 results about "Triiodothyronine" patented technology

The invention provides a chemoluminescence immunoassay measuring kit and a preparation method thereof for quantificationally detect triiodothyronine (T3) magnetic particles. The kit mainly comprises a triiodothyronine serial calibration sample, magnetic particle solution coated by an anti-fluorescein isothiocyanate (FITC) monoclonal antibody, a T3 antigen marked by biotin, T3 monoclonal antibody marked by FITC, streptavidin marked by alkaline phosphatase, chemoluminescence substrate solution and 20-time concentrated washing solution. The invention adopts a competitive-method reaction mode, effectively utilizes the chemoluminescence technology combined with magnetic particles and biotin-avidin immunity magnifying technology principle to quantificationally detect the content of T3 in blood serum and blood plasma samples of human bodies and ensure the sensitivity of the detection. The kit is simple, convenient, fast, sensitive and stable to use, and provides a very valuable detection method for clinic diagnosis and scientific research works.

The invention relates to a culture medium for a normal epithelial cell of a human or mammal, primary separation culture, subculture, 3D gas-liquid culture and 3D matrigel culture methods, the normal epithelial cell generated by using the culture medium and the culture methods and application of the normal epithelial cell to a toxicological evaluation system. The culture medium is prepared by mixing DMEM and Ham's F-12NUTRIENT MIX according to the volume ratio of 3:1 and also adding 4-6% of fetal calf serum, 1-3nM triiodothyronine, 0.4-0.65% of insulin-transferrin-selenium reagent, 4-6mu g / ml transferrin, 9-11ng / mL epidermal growth factors, 0.3-0.5mu g / mL hydrocortisone, 0.5-1.5nM cholera toxin, 0.4-0.6mu g / mL amphoterrible B, 35-45mu g / mL gentamicin, 45-55nM calpeptin, 35-45ng / ml recombinant human IL-1RA and 3mu g / ml recombinant human R-Spondin-1. The culture medium disclosed by the invention can be used for carrying out separation culture or subculture on the normal epithelial cell of the human or the mammal and any other various tissue source, rapidly proliferating the normal epithelial cell in vitro and establishing a cell line; and the normal epithelial cell is a normal diploid cell and is applied to the toxicological evaluation system of the human or the mammal.

The invention relates to an auxiliary diagnostic kit of thyroid diseases, and discloses a tetraiodothyroxide test kit which comprises tetraiodothyroxide testing particles, tetraiodothyroxide resisting antibody marked by biotin and disintegrating agent; wherein triiodothyronine testing particles are luminous particles coated by diiodothyronine, and the disintegrating agent is 8-anilino-1- naphthalenesulfonic acid ammonium salt hydrate. The invention also discloses a use method of a triiodothyronine kit. The kit can be combined with other serum and clinical information for the auxiliary diagnosis of thyroid diseases and the monitoring of the treatment effect of relevant patients.

The present invention provides, among other things, therapeutic compositions and methods that can effectively treat, slow or prevent a neurological disease (e.g., a neurodegenerative disease, e.g., mild cognitive impairment (MCI) or Alzheimer's disease (AD)), in particular, based on therapeutically effective amount of nifedipine, oxidized or nitroso nifedipine derivatives, lactam (e.g., a compound of formula (Ic) or (Ic-i), e.g., NFD-L1), thyroxine (T4), triiodothyronine (T3) and combinations thereof.

The invention belongs to the field of biochemistry detection, in particular to the measurement of bioactive molecules of neuroendocrine immunological network, particularly to a measurement method of amino bioactivators in organism (such as blood or brain tissue), in particular discloses a method for the single-time measuring of ten amino bioactivators in organism, which comprises: taking dansyl chloride as derivative reagent to perform single-time measuring on ten amino bioactivators such as epinephrine (E), noradrenalin (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), histamine (HA), gluetamic acid (Glu), aspartic acid (Asp), Gamma-aminobutyric acid (GABA), glycin (Gly) and trilute (T3) within 75min. The method is simple and rapid, can sensitively measure the concentrations of the ten amino bioactivators in organism fluid, and is suitable to the research requiring to completely analyze the ten amino bioactivators in the organism.

The invention discloses a thyroid function detection protein chip and a kit thereof. The kit comprises the thyroid function detection protein chip, wherein the chip is coated with a free triiodothyronine (FT3) antibody, a free thyroxine (FT4) antibody, a triiodothyronine (T3) antibody, a thyroxine (T4) antibody and a thyroid stimulating hormone (TSH) antibody. The kit can quantitatively detect the content of FT3, FT4, T3, T4 and TSH in patient serum, and is used for the auxiliary diagnosis of thyroid dysfunction diseases. Compared with the conventional enzyme linked immunosorbent assay (ELISA) technology, the chemiluminescence immunoassay keeps high specificity of the ELISA technology, stability and reliability of a detection result, and convenience of operation, and can improve detection sensitivity; and various indexes can be detected simultaneously, so time and cost are greatly saved.

The present invention provides, among other things, therapeutic compositions and methods that can effectively treat, slow or prevent mild cognitive impairment (MCI) or Alzheimer's disease (AD), in particular, based on therapeutically effective amount of nifedipine, oxidized or nitroso nifedipine derivatives, thyroxine (T4), triiodothyronine (T3) and combinations thereof.

The invention relates to a culture medium of amniotic fluid cells, which belongs to the field of biochemistry. The culture medium comprises the following components: basic medium, transferrin, sodium selenite, insulin, triiodothyronine, glucagons, basic fibroblast growth factor, hydrocortisone, testosterone, estradiol, progesterone and added antioxidant, wherein the antioxidant is a mixture of vitamin E and L-ascorbic acid; and the culture medium can be used in amniotic fluid cell culture after 4 to 15 volume percent of fetal calf serum is added in the culture medium, and a great amount of amniotic fluid cells which are at a division stage and meet the requirements of clinical diagnosis and scientific research can be obtained just within 7 to 8 days. The culture medium remarkably improves the culture effects of amniotic fluid cells and obviously increases cloning efficiency and cloning growth rate, reduces culture time, increases the success rate of cell culture, and fully plays the synergetic effects of vitamin E and L-ascorbic acid of different antioxidants.

The invention relates to a free triiodothyronine nanometer magnetic particle chemiluminescence assay kit and a preparation method thereof and a detection method thereof. The free triiodothyronine nanometer magnetic particle chemiluminescence assay kit comprises first reagents, second reagents and magnetic separation reagents, wherein the first reagents comprise fluorescein-labeled free triiodothyronine resistance antibodies, buffer solutions with potential of hydrogen(pH) 7-9, and the concentration of the fluorescein-labeled free triiodothyronine resistance antibodies is 0.5mug / mL-1mug / mL; the second reagents comprise an alkaline-phosphatase-labeled free triiodothyronine antigens, buffer solutions with pH 7-9, and the concentration of the alkaline-phosphatase-labeled free triiodothyronine antigens is 0.02 mug / mL-0.1mug / mL; the magnetic separation reagents are suspension liquid of magnetic particles covered with fluorescein-resistance antibodies. The free triiodothyronine nanometer magnetic particle chemiluminescence assay kit and the preparation method thereof and the detection method thereof have the advantage of enabling the free triiodothyronine to be quantificationally detected on the condition of lower cost, higher accuracy and higher precision.

The invention discloses a chemiluminescence quantitative detection kit for free triiodothyronine. The kit comprises a free triiodothyronine detection reaction plate, an enzyme conjugate, a luminescent substrate, a calibrator, a quality control material and washing concentrate, wherein the reaction plate is coated with a free triiodothyronine antibody. The kit can specifically and quantitatively detect the content of the free triiodothyronine in patient serum, and is used for the auxiliary diagnosis of thyroid dysfunction diseases. Compared with the conventional enzyme linked immunosorbent assay (ELISA) technology, the chemiluminescence immunoassay keeps high specificity of the ELISA technology, stability and reliability of a detection result, and convenience of operation, and can improve detection sensitivity simultaneously.

The invention discloses a kit for detecting free thyroxin by using magnetic particles as solid-phase carriers. The kit comprises magnetic particle suspension coupled with triiodothyronine-gelatin compound, free thyroxin series calibrator, horseradish peroxidase labeled antithyroidin antibody solution, luminous substrate liquid A and liquid B and concentration washing liquid. The invention also discloses a preparation method for the kit. The kit has the advantages that: because a magnetic particle chemical luminescent method is combined with a magnetic particle solid-phase coupling technique, the magnetic particles are suspended in the liquid in the absence of magnetic field, and the antigen antibody reaction is similar to homogeneous reaction; and the magnetic particles are convenient to separate under the action of an external magnetic field and quick to wash, and can improve the detection precision and stability when used as immunological carriers. The kit has the advantages of simple composition, convenient operation, stable and reliable result; and compared with the commercial elisa kits and plate-type chemical luminescent kits, the kit has the advantage that the reaction timeis only 1 / 8 to 1 / 4 of the time of the kits.

A method for selectively culturing the layer duck features that according to the regression relation between 7 biochemical genetic marker indexes of duck's blood (including serum thyroxin T4, 3, 5, 3'-triiodothyronine T3, IGF, BP, TP, GOT, and GPT), and the egg characters (including egg productivity, total egg weight and average egg weight), 5 of said indexes are chosen to create a regression equation, and the selective culture is then performed.

The invention discloses a culture medium that is used in transportation of tissue engineered skin. A basic culture fluid is added with additional hydrocortisone, epidermal growth factors, isoproterenol, insulin, transferrin, triiodothyronine, adenine, vitamin C, cholera toxin, L-serine, L-novain, palmic acid, linoleic acid, arachidonic acid, bovine pituitary extract, bovine serum albumin, penicillin, streptomycin and fungizone B. The obtained culture medium has strong stability and long shelf life of about 12 months, completely meet the demands of large-range transportation of the tissue engineered skin products, can effectively maintain the structure and functions of the tissue engineered skin in the transportation process, becomes solid after being cooled, and consequently has convenient carry in the transportation process.

The invention relates to an auxiliary diagnosis reagent kit of thyroid diseases, which discloses a triiodothyronine detection reagent kit. The triiodothyronine detection reagent kit comprises triiodothyronine detection particles, biotin marked anti-triiodothyronine antibodies and dissociation agents, wherein the triiodothyronine detection particles are diiodothyronine covered luminous particles, and the dissociation agents are 8-anilino group-1-ammonium naphthalenesulfenesulfonate. The invention also discloses a use method of the triiodothyronine detection reagent kit. The reagent kit of the invention can be combined with other blood serum and clinic information to be used for auxiliary diagnosis on the thyroid diseases and the monitoring on the relevant patient treatment effect.

The invention relates to a fluorescent microsphere joint examination device for five analytes of thyroid and a preparation method thereof and belongs to the field of medical examination equipment. A sample pad, an immunofluorescence crosslinked antibody glass fiber membrane, a nitrocellulose membrane and an absorbing pad are bonded to a plastic board; two ends of the nitrocellulose membrane are inlap joint respectively with the absorbing pad and the immunofluorescence crosslinked antibody glass fiber membrane; the other end of the immunofluorescence crosslinked antibody glass fiber membrane is in lap joint with the sample pad; the nitrocellulose membrane is provided with a detection line T and a quality control line C; thyroxine antibody, thyronine antibody and hormothyrin antibody are marked on the detection line; goat anti-rat IgG polyclonal antibody is spotted on the quality control line C. The fluorescent microsphere joint examination device for five analytes of thyroid and the preparation method thereof have the advantages that thyroxine, thyronine and hormothyrin antibodies are applied to coat the nitrocellulose membrane, high specificity is achieved, T3 antibody, T4 antibody, TSH antibody, free T3 antibody and free T4 antibody can be simultaneously detected in a specimen, and the device herein is simple to operate and highly practical.

The invention discloses an eccrine sweat gland cell induction medium and an application thereof. The eccrine sweat gland cell induction medium comprises a DMEM / F12 cell medium, a keratinocyt medium, fetal calf serum, an epidermal growth factor, triiodothyronine, hydrocortisone, insulin-transferrin-sodium selenite, L-glutamine, penicillin, streptomycin, a hepatocyte growth factor and a bone morphogenetic protein 4. The eccrine sweat gland cell induction medium disclosed by the invention can be used for successfully inducing stem cells to be directionally differentiated to eccrine sweat gland-like cells so as to provide sufficient eccrine sweat gland cells for treating extensive burn patients, so that the living quality of the patients is greatly improved. The medium further provides a theoretical basis for researching differentiated development of the sweat gland, so that the medium has a potential clinical application prospect.

The invention provides implantable drug delivery devices comprising a core comprising a polymer (or polymer blend) and one or more drugs or pharmaceutical substances, and an outer shell comprising a polymer (or polymer blend) and one or more porogen materials. The invention reduces burst release of drug. Pharmaceuticals such as triiodothyronine (T3) or ropinirole can be delivered by the devices.

The present invention provides, among other things, therapeutic compositions and methods that can effectively treat, slow or prevent a neurological disease (e.g., a neurodegenerative disease, e.g., mild cognitive impairment (MCI) or Alzheimer's disease (AD)), in particular, based on therapeutically effective amount of nifedipine, oxidized or nitroso nifedipine derivatives, lactam (e.g., a compound of formula (Ic) or (Ic-i), e.g., NFD- L1), thyroxine (T4), triiodothyronine (T3) and combinations thereof.

The invention discloses a formula of cell factor and compound and action to regenerate nerve and diagnose the nerve system, which is fit for insuline, bovine serum albumin, putrescine, hydrocortisone, luteohormone, transferrin, sodium selenite, alkaline cellulose cell growing factor and epiderm growing factor.

The invention discloses a culture fluid that promotes the generation of a lipid structure of tissue engineered skin. A basic culture fluid is added with hydrocortisone, epidermal growth factors, isoproterenol, insulin, transferrin, triiodothyronine, adenine, vitamin C, tretinoin, cholera toxin, L-serine, L-novain, palmic acid, linoleic acid, arachidonic acid, bovine pituitary extract, bovine serum albumin, calcium chloride, penicillin, streptomycin and fungizone B, etc. Tissue engineered skin with mature and completely differentiated horny layer and good lipid structure can be obtained by cultivating keratinocyte in the culture fluid of the invention, the culture fluid has strong stability, long shelf life of about 12 months, simple and convenient preparation method, and convenient product synthesis and mass production.

The invention relates to the field of cytobiology, and particularly relates to a sweat gland differential induction medium and applications thereof. The sweat gland differential induction medium is prepared by adding the following components into a low-sugar DMEM medium: based on the volume of the low-sugar DMEM medium, 8-15% by volume of fetal calf serum, 0.3-0.5mu g / ml of semi-hydrocortisone succinate, 10-100ng / ml of rh-KGF, 1-3nmol / ml of triiodothyronine, 10-100ng / ml of rh-EGF, and 0.05-0.2ml / ml of an insulin-transferrin-sodium selenite solution. The sweat gland differential induction medium is constructed by taking a recombinant human keratinocyte growth factor as a key regulation factor, constructing the finished product of the sweat gland differential induction medium, inducing the differentiation of human umbilical cord mesenchymal stem cells to sweat gland sample cells. By using the medium disclosed by the invention, the result is stable and reliable, and the sweat gland differential induction medium can be widely used for related studies and clinical application of differential induction of various MSCs to the sweat gland cells.

This invention relates to quantitatively triiodothyronine chemical light analysis agent box, which belongs to clinical blood immune testing technique field and comprises the following parts: non-transparent polyphony lacetylene board with the gland antigen, prostate antigen series standard product, enzyme mark gland specific antigen, lighting bottom object A liquid composed of effective light agent, compound strengthening agent and amino acid and B liquid composed of amino acid oxidase and stabilizer.

The invention discloses a preparation method of an animal model with oral ulcer caused by fire excess from yin deficiency. The method comprises selecting healthy rats, carrying out subcutaneous injection on the rats with a triiodothyronine solution according to the dosage of 5-200 micrograms per kilogram for 14-21 days, carrying out impregnation on a cotton ball with phenol, and firing the rat lateral buccal mucosa to form a white wound so that a desired animal model can be obtained after 24-48h. Compared with the prior art, the method is used for preparation of an animal model with oral ulcercaused by fire excess from yin deficiency. The model can induce the oral ulcer based on the fire excess from yin deficiency according to the traditional Chinese medicine differentiation of symptoms and signs, well satisfies clinical practice, has high feasibility and high applicability and has a great application value in heat and fire removal, tissue damage reduction and ulcer healing promotion.

The invention discloses a micro-fluidic chemiluminescence detecting system for magnetic particles for detecting thyroid functions. The system comprises a bottom plate and an upper chip, and the upperchip comprises a sample adding area in the center of the upper chip and five micro-fluidic reaction detecting channels communicated with the sample adding area, wherein the five micro-fluidic reactiondetecting channels include a total triiodothyronine micro-fluidic reaction detecting channel, a total thyroxine micro-fluidic reaction detecting channel, a free triiodothyronine micro-fluidic reaction detecting channel, a free thyroxine micro-fluidic reaction detecting channel and a thyroid stimulating hormone micro-fluidic reaction detecting channel. In application of the system, the bottom plate is arranged under the upper chip, and magnets which are permanent magnets or electromagnets are arranged at positions, corresponding to magnetic particle coating areas of the micro-fluidic reactiondetecting channels, of the bottom plate. By ingenious combination of the micro-fluidic technology and the magnetic particle chemiluminescence technology, quickness, high sensitivity and accuracy in quantitative detection of target substances are realized.

The invention provides a kit used for determination of triiodothyronine. The invention aims to overcome the problems of poor specificity and poor sensitivity of conventional T3 immunoassay reagents. The kit used for determination of triiodothyronine comprises magnetic beads coated by a goat monoclonal antibody, an ANS dissociation agent and an enzyme operation fluid, wherein the magnetic beads coated by the goat monoclonal antibody is labeled with biotin. The kit provided by the invention employs the magnetic beads coated by the goat monoclonal antibody; magnetic particles (the magnetic beads) are coated by anti-biotin antibody which is the goat monoclonal antibody; so the kit has higher specificity and sensitivity compared with the conventional T3 immunoassay reagents.

The invention relates to a culture medium of amniotic fluid cells, which belongs to the field of biochemistry. The culture medium comprises the following components: basic medium, transferrin, sodium selenite, insulin, triiodothyronine, glucagons, basic fibroblast growth factor, hydrocortisone, testosterone, estradiol, progesterone and added antioxidant, wherein the antioxidant is a mixture of vitamin E and L-ascorbic acid; and the culture medium can be used in amniotic fluid cell culture after 4 to 15 volume percent of fetal calf serum is added in the culture medium, and a great amount of amniotic fluid cells which are at a division stage and meet the requirements of clinical diagnosis and scientific research can be obtained just within 7 to 8 days. The culture medium remarkably improves the culture effects of amniotic fluid cells and obviously increases cloning efficiency and cloning growth rate, reduces culture time, increases the success rate of cell culture, and fully plays the synergetic effects of vitamin E and L-ascorbic acid of different antioxidants.

The invention relates to an aminotic cell culture medium, which is prepared from a basic culture medium, and further is prepared from the following component: transferrin, sodium selenite, insulin, triiodothyronine, glucagon, progesterone, hydrocortisone, testosterone, estradiol, a basic fibroblast growth factor, vitamin E, vitamin C, antibiotic and fetal calf serum. The invention also relates toapplication of the aminotic cell culture medium in antenatal diagnosis of aminotic cell growth and an aminotic cell culture method. The aminotic cell culture medium provided by the invention has theadvantages of capability of promoting aminotic cell proliferation and excellent quality of the aminotic cell culture medium.

The invention discloses a free triiodothyronine measurement kit and a fabrication method thereof, and belongs to the technical field of vitro inspection. By the free triiodothyronine measurement kit,the technical problems of long reaction time, slow measurement speed, narrow of linear range and hook effect easy to generate of the prior art when a tested substance is high in concentration are solved. The kit comprises the following reagents of a solid-phase reagent 1 and a liquid-phase reagent, wherein the solid-phase reagent R1 is a suspension liquid including a T3 analogue-coated paramagnetism microsphere, the liquid-phase reagent R2 is a suspension liquid including an acridinium ester labeled T3 antibody, the paramagnetism microsphere in the R1 is Fe3O4 with a surface wrapped by a carboxyl active group, and the particle size of the paramagnetism microsphere is 0.1-5 micrometers. With the free triiodothyronine measurement kit and the fabrication method thereof, provided by the invention, free triiodothyronine in blood / plasma is quantitatively detected by a chemiluminescence immunity analysis method, the kit has the advantages of high sensitivity, rapid detection, wide linear range, low cost and the like and is simple and convenient to operate, and automation is facilitated.