13 results about "Triiodothyronine" patented technology

The invention discloses lignin-triiodothyronine self-assembled particles as well as a preparation method and application thereof. The lignin-triiodothyronine composite nano particles are prepared from lignin and triiodothyronine in a joint self-assembly mode. Experiments prove that single in-situ injection of the lignin-triiodothyronine composite nanoparticles can increase the expression of subcutaneous adipose tissues UCP-1 of normal diet fed mice and reduce adipose cells; single in-situ injection can increase the expression of subcutaneous adipose tissue UCP-1 of a mouse fed with short-term high-fat diet, reduce the weight of adipose tissue, increase the energy consumption of the mouse and improve the glucose tolerance of the mouse; multiple times of in-situ injection can improve obesity and fatty liver caused by long-term high-fat diet. The lignin-triiodothyronine composite nanoparticle provided by the invention has a good application prospect in treating, relieving or improving obesity or metabolic diseases related to the obesity.

The application discloses a kind of serum-free epidermal melanocyte culture medium and its application, method includes by dissolving adenine, hepatocyte growth factor (HGF), glutamine, retinoic acid, vitamin E, nicotinic acid, sodium selenite, sodium hyaluronate and recombination human type XVII collagen are configured into initial culture medium;Preparation of melanocyte stimulating hormone, endothelin-3, transferrin, triiodothyronine, cholesterol, neuroregulin 1 (NRG1), 3-isobutyl-1-methyl purine (IBMX), dibutyryl cyclic adenosine monophosphate (db-cAMP), cholera toxin, hydrocortisone and recombination human insulin mother liquor;Step three: according to culture and concentration, a certain amount of mother liquor of step two is added to initial culture medium.The culture medium can effectively promote human epidermal melanocyte proliferation, melanin accumulation and help to promote human umbilical cord mesenchymal stem cells into human epidermal melanocyte, provide key advantage for obtaining functional melanocyte with high activity and therapeutic potential.

The invention relates to the technical field of stem cell preparation, and provides a nephrogenic stem cell culture medium and a preparation method of nephrogenic stem cells. Wherein the kidney-derived stem cell culture medium takes DMEM / F-12 and fetal calf serum as a basic culture medium, and human serum albumin, triiodothyronine, fibroblast growth factors, parathyroid gland hormone, L-glutamine, squalene and caulis spatholobi polysaccharide are added. The components in the kidney-derived stem cell culture medium synergistically form a closed loop of'nutrition supply-metabolism regulation-cell protection ', basic nutrition and serum provide a material basis, hormone and growth factors improve metabolism and proliferation activity, squalene and caulis spatholobi polysaccharide guarantee stability of cells under high-activity metabolism, and the cell protection effect is improved. And finally, efficient proliferation and stemness maintenance of the stem cells are realized.

The invention belongs to the technical field of biological detection, and particularly relates to a free triiodothyronine fluorescence immunochromatography detection test strip, a preparation method thereof and a kit containing the free triiodothyronine fluorescence immunochromatography detection test strip. According to the free triiodothyronine fluorescence immunochromatography detection test strip provided by the invention, a double-antibody sandwich method is combined with a time-resolved fluorescence immunochromatography technology, so that the problem of insufficient detection sensitivity caused by small molecular weight of free triiodothyronine is effectively solved. Compared with a traditional competition method, the double-antibody sandwich method has higher sensitivity, and accurate detection of pmol / L-level low-concentration fT3 in blood can be realized. Meanwhile, by introducing a time-resolved fluorescent microsphere labeling technology, the interference of background fluorescence is remarkably reduced, and the detection stability is further improved. In addition, the free triiodothyronine fluorescence immunochromatography detection test strip provided by the invention can be used for testing different sample types of whole blood, serum and plasma, the sampling amount is small, and the detection requirements of various application scenes can be met.

The utility model discloses a thyroid function detection assembly. The thyroid function detection assembly comprises a whole blood separation piece and at least one piece of detection test paper, the whole blood separation part is used for filtering red blood cells in blood; the detection test paper comprises a sample receiving end and a detection part, the whole blood separation part is connected with the sample receiving end, and the detection part is used for detecting the concentration of one of thyroid stimulating hormone, free triiodothyronine and free thyroxine. According to the thyroid function detection assembly, the thyroid function can be detected, during detection, the whole blood separation piece can rapidly separate red blood cells in blood within a short time, the rest of the separated blood can enter the detection test paper, detection can be completed by observing the detection part, the blood does not need to be centrifuged into serum, and the detection efficiency is improved. Direct detection of the whole blood sample can be realized, the detection cost is lower, the operation is simple and convenient, the detection time is greatly saved, and the detection efficiency is improved.

This invention discloses an emergency product and method for hypothermia protection in low-temperature environments, belonging to the field of hypothermia protection and emergency care. This invention is the first to apply triiodothyronine (T3) to the preparation of an emergency product for hypothermia protection in low-temperature environments, fully utilizing T3's ability to rapidly increase heat production in tissues such as brown adipose tissue, thereby enhancing the body's active heat production capacity. Combining the physiological metabolic characteristics of the human body with the pharmacological properties of T3, this invention has achieved excellent results in animal experiments. On the one hand, T3 can maintain the core body temperature of hypothermic experimental animals at a higher level for a longer period, resulting in increased swimming time and extending the golden time for rescue. On the other hand, it can effectively improve the recovery of body temperature after rescue, significantly increasing the success rate of treatment. However, when using T4, which is considered more effective in conventional clinical settings, no significant improvement in these two aspects was observed, fully demonstrating the specificity and superiority of T3.

The invention relates to the technical field of in-vitro diagnosis, in particular to a thyroid hormone detection kit and a thyroid hormone detection method. Wherein the thyroid hormone comprises at least one of triiodothyronine and tetraiodothyronine; the thyroid hormone detection kit comprises (i) a cyclodextrin reserve reagent; ii) an antibody capture reagent; iii) an antibody detection reagent; and, iv) a signalling agent. According to the kit and the detection method, the T3 and T4 detection signal intensity can be improved, the detection limit is reduced, and the accuracy and reliability of low-concentration sample detection are improved.

The invention discloses a construction method and application of a refractory pituitary tumor organ. The method comprises the following steps: culturing pituitary tumor tissues by using a pituitary tumor organoid culture medium, the pituitary tumor organoid culture medium comprises a basic culture medium and an additive, and the additive comprises triiodothyronine and / or thyroid stimulating hormone releasing hormone. The organoid constructed by the method can stably express pituitary tumor markers such as SF1 / FSH / synapsin and accurately simulate clinical drug resistance characteristics (such as temozolomide resistance) in vitro, solves the problems that primary tumor cells are low in activity and drug resistance phenotypes are difficult to maintain, fills the blank that no reliable in-vitro model exists in the field, and has good application prospects. And a platform is provided for drug resistance mechanism research and individualized drug screening.

The invention discloses a free triiodothyronine fluorescence immunochromatography detection kit and a preparation method thereof. The detection kit comprises a reagent card and a diluent, the reagent card comprises a bottom plate as well as a sample pad, a combination pad, a nitrocellulose membrane and absorbent paper which are sequentially erected on the bottom plate, the combination pad is coated with a fluorescent microsphere labeled FT3 monoclonal antibody and a fluorescent microsphere labeled goat anti-chicken IgY antibody, and the mass ratio of the fluorescent microsphere labeled FT3 monoclonal antibody to the fluorescent microsphere labeled goat anti-chicken IgY antibody is 10: (15-35); the nitrocellulose membrane is provided with a detection line coated with an FT3 capture antibody and a quality control line coated with a chicken IgY (Immunoglobulin Y). The detection kit has the advantages of excellent sensitivity and specificity, good stability of detection results, and good application prospects.

Provided herein are maturation media and methods for maturing early embryonic human heart organoids into mature human heart organoids. The maturation media comprises a cell growth medium comprising a media supplement comprising: one or more fatty acids, triiodothyronine (T3) growth hormone, insulin, one or more antioxidants, a sugar, and carnitine; one or more additional fatty acids; additional carnitine or creatine; and additional T3 growth hormone. The maturation media can also comprise one or more additional sugars, additional antioxidants, and growth factors. The methods comprise contacting an early embryonic human heart organoid with one or more maturation media to produce a mature human heart organoid.

The invention provides an application of a biomarker in preparation of a product for evaluating the prognosis effect of chronic plus acute hepatic failure. The application comprises the following steps: a) a detection reagent is adopted to determine characteristic data of a biomarker in a biological sample of a patient suffering from chronic plus acute hepatic failure, the biomarker comprises thyroid hormone, and the thyroid hormone comprises trans-triiodothyronine; b) outputting the prognosis result of the patient with the chronic and acute hepatic failure based on the characteristic data of the biomarker. The problem of poor accuracy of evaluating the prognosis effect of the chronic and acute hepatic failure in the prior art can be solved, and the method is suitable for the technical field of biological detection.

The invention discloses a serum-free additive E6 for a chicken primordial germ cell culture medium and application of the serum-free additive E6, and belongs to the technical field of animal husbandry and biology. The E6 serum-free additive is composed of six components necessary for in-vitro proliferation of chicken PGCs, and specifically comprises DL-alpha-tocopheryl acetate, BSA (fatty acid-free component V), human recombinant insulin, corticosterone, linolenic acid and triiodothyronine. After the E6 serum-free additive is added into a chicken PGCs culture medium, the basic form of cells can be effectively maintained, long-term in-vitro proliferation of the cells is supported, expression of specific marker genes and proteins such as reproduction and pluripotency is maintained, and meanwhile the long-term subculture capacity and gonad migration potential of the PGCs are guaranteed. The E6 serum-free additive disclosed by the invention has the advantages of simple and clear components, simplicity and convenience in preparation and relatively low cost, and can be widely applied to the fields of poultry germplasm resource preservation, gene editing, biological breeding and the like.

A kind of cascade lipolysis-oxidation nano reactor and preparation method thereof, medical device and application.Nano reactor has core-shell structure, its core is loaded triiodothyronine mesoporous polydopamine particle, shell is the mesoporous silica layer of surface fixed lipase.The structure retains the catalytic activity of lipase on the surface of particle while achieving T3 slow release;Lipase is stably anchored in the shell by amination and glutaraldehyde crosslinking, and the final surface Zeta potential is-17.5±0.5 mV.The present application further loads the nano reactor in the microneedle patch of gamma-polyglutamic acid matrix for local delivery of subcutaneous adipose tissue.It has been verified that the system can synergistically activate triglyceride hydrolysis and mitochondrial fatty acid oxidation, significantly reduce lipid droplet accumulation, improve mitochondrial membrane potential, up-regulate lipolysis and oxidation related gene expression, effectively restore the lipid turnover function of obese fat depot, and provide a solution for local intervention of obesity and related metabolic disorders.