The invention discloses a complete medium for cultivating cPGCs (chicken primordial germ cells) and a using method of the complete medium. The complete medium consists of KO-DMEM, B-27supplement, NAA, nucleosides, GlutaMAX II, [beta]-mercaptoethanol, ovalbumin, OVT, heparin sodium, Activin A, TGF-[beta]1, BMP4, FGF-2 and IGF-1. The using method comprises the following steps: cultivating collected 14HH chicken embryo blood in a culture system containing a cPGCs complete culture solution, and conducting primary culture for 10-15d so as to obtain purified cells; (2) adding the purified cPGCs in the cPGCs complete medium once again and conducting in-vitro culture and amplification; and (3) conducting counting and indirect immunofluorescence detection on the cPGCs obtained from the in-vitro culture and amplification, including SSEA-1 and DAZL surface markers . The medium provided by the invention, different from a conventional culture method which has serum and a feed layer, is concrete in ingredients, and the medium can facilitate researches on a molecular mechanism of cell differentiation.