11 results about "Avian embryo" patented technology

The present disclosure relates to deoxyribonucleic acid (DNA) editing agents, and their use in preparing genetically modified cells and birds. The present disclosure further relates to fertile genetically modified avians and genetically modified avian primordial germ cells (PGCs) for producing sterile genetically modified avians (birds) that can serve as surrogate hosts for donor PGCs. The present disclosure further relates to methods for producing fertile avian strains that can produce a population of embryos and offspring, in both sexes, all of which are sterile and viable, and further relates to their subsequent use as sterile surrogate hosts for donor PGCs.

To provide a method for culturing avian embryos outside the eggshell that can reliably achieve high embryo survival rates and low embryonic developmental abnormality rates, and that is relatively easy to perform, as well as a method for evaluating the toxicity of test substances using such a culture method. [Solution] The embryo and yolk are removed from the fertilized bird egg and transferred together with avian aqueous oocyte white into a roughly cylindrical well in a culture vessel. The opening of the well, which is filled with avian aqueous oocyte white, is then covered with a porous membrane, and the avian embryo is cultured by turning it over. Preferably, the cultured avian embryo is further cultured in a roughly cylindrical well in which a porous membrane is provided along the inner circumference, and the amount of avian aqueous oocyte white is adjusted so that there is a space between the well and the avian yolk below the opening.

The application discloses an avian embryo blood vessel injection device, which comprises a rack, a visual illumination module, an injection execution mechanism and an avian embryo egg fixing platform. The fixing platform comprises a supporting table and a plurality of self-adaptive clamping units. The clamping unit comprises a support which is in sliding cooperation with a sliding groove of the supporting table, a first driving mechanism which drives the support to move, a flexible constraint member which is arranged on the support, a second driving mechanism which adjusts the tension degree of the flexible constraint member, and a force sensing unit which detects the clamping pressure. The injection execution mechanism comprises a base, a multi-joint motion mechanism, a driving assembly and an end effector. The fixing platform and the injection execution mechanism are integrated, and the multi-joint motion mechanism is arranged in the injection execution mechanism. The stable support of the avian egg and the space adjustment of the injection path are realized. The angle-adjustable fine puncture of the avian embryo blood vessel can be realized in the fixed state. The positioning stability and the operation repeatability in the injection process are improved. The automation degree and the precision of the blood vessel injection are improved.

The invention relates to the process of extracting a biological sample from a poultry egg (6, 106), in particular for the in-ovo sex determination of a poultry embryo. The invention proposes, in a system (2, 102) in question, a receiving means (4, 104) for receiving at least one poultry egg (6, 106) from which the sample is to be extracted; and a sampling device (8, 108) which is designed to extract a biological sample (12, 112) from an extraction region (10, 110) of the poultry egg (6, 106), said sampling device (8, 108) having a penetration and extraction means (16, 116) which is designed to penetrate the shell (18) of the poultry egg (6, 106) and extract the sample (12, 112) from the extraction region (10, 110) and said receiving means (4, 104) having an aligning device (20, 120) which is designed to position the poultry egg (6, 106) and / or the penetration and extraction means (16, 116) relative to each other such that the penetration and extraction means (16, 116) is aligned adjacently to the extraction region (10, 110).

The present invention relates to a measuring system for the non-invasive, preferably automated, sex determination of embryos in an egg 50 during early embryonic development, particularly before the seventh day of incubation, particularly during incubation, particularly in an incubator with at least one egg tray 40 for holding a plurality of eggs 50 and at least one egg trolley 80 for holding at least one egg tray 40, comprising at least one irradiation unit 10 for irradiating an egg 50 with electromagnetic radiation; at least one sensor unit 20 for detecting electromagnetic radiation transmitted by the egg 50; and an evaluation unit 30 with at least one spectrometer 31 configured to receive the radiation transmitted by the egg 50 and to generate a spectrum of the radiation transmitted by the egg 50.and a data processing unit 33 configured to receive spectra generated by the spectrometer 31 and to store them as a reference spectrum or measurement spectrum; an identification unit 23 for generating identification data by means of which the spectra generated on an egg 50 can be uniquely assigned to the egg 50; and a classification unit 35; wherein the data processing unit 33 is configured to store spectra generated by the spectrometer 31 and associated identification data, and wherein the classification unit 35 is configured to determine the sex of the embryo based on at least one reference spectrum and at least one measurement spectrum. A corresponding method is further specified within the scope of the invention.

The invention relates to a measuring system. The invention relates to a sex detection device for non-invasive, preferably automated sex detection of embryos in eggs (50) in early embryonic development, in particular before the seventh incubation day, in particular during incubation, in particular in an incubator having at least one egg tray (40) for receiving a plurality of eggs (50) and at least one egg carriage (80) for holding the at least one egg tray (40), the measuring system has at least one irradiation unit (10) for irradiating the egg (50) with electromagnetic radiation; at least one sensor unit (20) for detecting electromagnetic radiation transmitted through the egg (50); an evaluation unit (30) having at least one spectrometer (31) configured to receive the radiation transmitted through the egg (50) and to generate a spectrum of the radiation transmitted through the egg (50); and a data processing unit (33) configured to receive the spectrum generated by the spectrometer (31) and to store the spectrum as a reference spectrum or a measurement spectrum; an identification unit (23) for generating identification data, by means of which the spectrum generated on the egg (50) can be individually assigned to the egg (50); and a classification unit (35); wherein the data processing unit (33) is configured to store the spectrum generated by the spectrometer (31) and the associated identification data, and the classification unit (35) is configured to determine the gender of the embryo on the basis of at least one reference spectrum and at least one measurement spectrum. The invention further relates to a corresponding method.

The invention relates to a method for preparing avian eggs for the purpose of characterizing an avian embryo contained within an egg. The method comprises the following steps carried out in a preparation station (4) after a period of incubation in a hatchery: - positioning an egg horizontally on a support, the longitudinal axis of the egg being parallel to the support on which it rests; - leaving the egg thus positioned to allow the embryo to migrate upwards along its longitudinal axis. Figure for the abstract: No figure

The invention relates to a process for isolating embryonic stem cells (100) from at least one avian embryonic cells comprising: isolating at least one embryo (101) at a developmental stage around oviposition; suspending embryonic cells (102) obtained by dissociating embryo(s) of step a) in an animal serum free basal culture medium supplemented with a combination of growth factors, at least one inhibitor and a substitute to animal serum; seeding the suspension of embryonic cells (103) on a layer of feeder cells; culturing (104) the embryonic cells for at least one passage.

This invention provides chimeric pheasant bird embryos, methods for obtaining them, and novel animal models that are particularly useful for screening immunotherapeutic agents and / or cells, as well as for classifying patients. [Solution] A chimeric pheasant avian embryo is provided, comprising both types of exogenous cells: a. a population of at least one type of cancer cells and b. a population of at least one type of immune cells, wherein the exogenous cancer cells are present in at least one tissue of the embryo and the exogenous immune cells are present in at least one tissue of the embryo and / or circulate in the blood vessels of the embryo.

The present invention relates to a process for the non-destructive determination of gender, developmental stage and / or viability of an avian embryo in an egg, comprising (a) detecting at least a first developmental marker compound selected from sugars and / or amino acids, precursors and metabolites thereof in an egg at a time period of from the beginning of the incubation of the egg until the hatching; (b) measuring the amount of the at least first detected developmental marker compound, and (c) comparing the amount to a base line established for male and female, developmental stage of the embryo, and / or alive and deceased or non-developed embryo, to determine whether the embryo is viable, male and / or female, and / or the developmental stage of the embryo.

Provided are methods of producing avian embryonic stem cells. In certain embodiments, the methods comprise culturing epiblast cells obtained from a stage IX-XI avian embryo in a cell culture medium present in a feeder-free cell culture container. Also provided are methods of producing two-dimension cultures, spheroids, or organoids from the avian embryonic stem cells produced according to the methods. For example, provided are methods of producing avian telencephalic organoids from the avian embryonic stem cells. Also provided are methods of using the avian embryonic stem cells, spheroids and organoids produced according to the methods of the present disclosure. For example, provided are screening methods comprising contacting the avian embryonic stem cells, spheroids or organoids with a test agent, and assessing for an effect of the test agent on the avian embryonic stem cells, spheroids or organoids.