To provide a method for culturing avian embryos outside the
eggshell that can reliably achieve high
embryo survival rates and low embryonic developmental
abnormality rates, and that is relatively easy to perform, as well as a method for evaluating the
toxicity of test substances using such a culture method. [Solution] The
embryo and
yolk are removed from the fertilized
bird egg and transferred together with avian aqueous
oocyte white into a roughly cylindrical well in a
culture vessel. The opening of the well, which is filled with avian aqueous
oocyte white, is then covered with a
porous membrane, and the
avian embryo is cultured by turning it over. Preferably, the cultured
avian embryo is further cultured in a roughly cylindrical well in which a
porous membrane is provided along the inner circumference, and the amount of avian aqueous
oocyte white is adjusted so that there is a space between the well and the avian
yolk below the opening.