Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture method for differentiating primordial germ cells into functionally mature oocytes

A technology of primordial germ cells and oocytes, applied in the field of oocyte culture

Pending Publication Date: 2018-08-03
TOKYO UNIVERSITY OF AGRICULTURE +2
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, there has been no report on a method of successfully forming functional eggs from primordial germ cells or primordial germ cell-like cells by culturing them in vitro without using a living body

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture method for differentiating primordial germ cells into functionally mature oocytes
  • Culture method for differentiating primordial germ cells into functionally mature oocytes
  • Culture method for differentiating primordial germ cells into functionally mature oocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0139] In the following examples, all animals were purchased from Japan CLEA Corporation. 12.5 dpc embryos of BDF1 mice were obtained by mating DBA / 2J male mice with C57BL / 6N female mice. In addition, as a control area, BDF1 (C57BL / 6N×DBA / 2J cross) 10-day-old and adult female mice were used. In addition, in the following examples, male BDF1 mice were used as sperm donors. In addition, when breeding pseudopregnant female mice, ICR female mice were mated with ligated ICR male mice. All experiments were approved by the Animal Experiment Committee of Tokyo University of Agriculture.

[0140] (1. In vitro organ culture of gonads)

[0141] Relative to the basal medium, the medium used for organ culture was supplemented with FBS (Gibco, American Life Technologies), SPS (SAGE in vitro fertilization), KSR (Gibco, American Life Technologies) at the concentration of each test area below. company), estrogen receptor antagonist ICI 182,780 (7α,17β-[9-[(4,4,5,5,5-pentafluoropentyl)sulfi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention addresses the problem of providing a method for differentiating primordial germ cells into functionally mature GV stage oocytes by in vitro culture. The present invention pertains to a method for differentiating primordial germ cells into functional GV stage oocytes in vitro including (a) a step for forming secondary follicles by culturing primordial germ cells and feeder cells adjacent to the primordial germ cells under conditions that eliminate the effects of estrogen or factors having a similar function to estrogen, (b) a step for partially cleaving the bonds between the granulosa cell layer and the thecal cell layer among the oocyte, granulosa cell layer, and thecal cell layer that constitute the formed secondary follicles, and (c) a step for differentiating the oocytes into functional GV stage oocytes by culturing the oocytes and granulosa cell layer that constitute the secondary follicles and the thecal cell layer in medium including a polymer compound.

Description

technical field [0001] The present invention relates to culture methods for differentiating primordial germ cells into functionally mature oocytes. Background technique [0002] Only germ cells are able to pass on genetic information to the next generation. Moreover, the ovum in the germ cells plays the role of initiating development and guiding the early development, so the ovum is responsible for a very important main role. Numerous primordial germ cells at the stage of somatic cell division are present in the embryonic gonads of female mammals. Before birth, they both switch to meiosis and differentiate into oocytes. After shifting to meiosis, oocytes cannot proliferate again, and oocytes in the ovary are in an arrested developmental stage. Depending on the animal species' unique estrous cycle and ovulation times, only a fraction of the oocytes grow and mature. Therefore, the number of fertile eggs produced by a female during her lifetime is very small compared to the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/075C12N5/0735C12N15/09
CPCC12N2501/392C12N2501/998C12N2506/04C12N5/0609C12N2501/31C12N2501/11C12N2500/50C12N5/0611C12N2506/02C12N2506/45C12N2501/727C12N2501/235C12N2501/16C12N2501/115C12N2533/52C12N2501/155C12N2501/125C12N2501/19C12N2500/30C12N2506/24C12N5/0604C12N2500/90C12N2501/30C12N15/09
Inventor 尾畑弥生平尾雄二林克彦
Owner TOKYO UNIVERSITY OF AGRICULTURE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com