15 results about "In ovo" patented technology

The invention discloses a method for establishing a cell line by serum-free culture of PGCs (Primordial Germ Cells) of breeding poultry, the cell line is prepared by blending calcium-free DMEM (Dulbecco Modified Eagle Medium), water and Knockout-DMEM, the cell line is more suitable for osmotic pressure and calcium ion concentration required by proliferation of the PGCs of the breeding poultry, various components of serum-free culture are perfected by using fresh egg white, and the cell line is more suitable for proliferation of the PGCs of the breeding poultry. The combination of growth factors required by primordial germ cells is optimized by adding the FGF-1, so that 100% of cell line establishment efficiency and rapid growth of cells from reproduction crescent, blood and gonad tissues of chicken embryos are realized, and a technical basis is provided for poultry frozen breed conservation, stem cell propagation and gene editing operation.

The invention belongs to the field of chicken hatching egg sex identification, and particularly relates to a method for identifying the sex of a chicken hatching egg by fusing a visible light image and hyperspectral data. Comprising first-stage identification and second-stage identification, in the first-stage identification, transmission light is combined with a YOLO intelligent algorithm, and fertilized egg screening and primary sex classification are achieved by identifying hatching egg blood vessel form distribution; in the second-stage identification, the form and biochemical information of the hatching eggs is collected by adopting a hyperspectral technology for secondary gender identification; if the two identification results are consistent, the female hatching eggs continue to be hatched, and the male hatching eggs are removed; if the two identification results are inconsistent, corresponding hatching eggs are collected for independent hatching. Experimental results prove that based on a two-stage method of YOLO recognition and visible light image and hyperspectral data fusion, the average accuracy of hatching egg gender classification reaches 98.9%, and an effective scheme is provided for early gender identification of chicken embryos.

To provide a convenient, rapid, and reliable method for determining one or more properties of a bird egg, especially a chicken egg, and / or one or more properties of a bird embryo inside the egg, especially a chicken embryo. [Solution] A non-invasive method for determining one or more properties of a bird egg, particularly a chicken egg, and / or one or more properties of a bird embryo inside the egg, particularly a chicken embryo, using near-IR spectroscopy is described herein. More specifically, a non-invasive method for determining the sex of a bird embryo inside a bird embryo, particularly a chicken embryo, is described herein. Furthermore, a system for non-invasively determining one or more properties of a bird egg, particularly a chicken egg, and / or one or more properties of a bird embryo inside the egg, particularly a chicken embryo, is described herein, as is the use of a spectrometer selected from a multi-channel spectrometer, a miniature diffraction grating spectrometer, and a monolithic miniature spectrometer, and in particular a computer program therefor, in the system and / or method described herein.

1. The name of the design product: shell-less chicken embryo incubation bearing device. 2. The use of the design product: for shell-less chicken embryo incubation. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

A composition, an in ovo vaccine, and a composition for use as a vaccine against coccidiosis. The compositions and vaccine include Eimeria oocysts from a precocious attenuated strain of E. acervulina, and from at least one precocious attenuated strain selected from E. maxima, E. praecox and E. tenella. The composition and vaccine can be administered in ovo to embryonated eggs of a domesticated bird. A combination vaccine for use in the prevention and / or treatment of an Eimeria infection and Infectious Bursal Disease (Gumboro disease) and a kit for use as a vaccine including the composition.

The present invention relates to a measuring system for the non-invasive, preferably automated, sex determination of embryos in an egg 50 during early embryonic development, particularly before the seventh day of incubation, particularly during incubation, particularly in an incubator with at least one egg tray 40 for holding a plurality of eggs 50 and at least one egg trolley 80 for holding at least one egg tray 40, comprising at least one irradiation unit 10 for irradiating an egg 50 with electromagnetic radiation; at least one sensor unit 20 for detecting electromagnetic radiation transmitted by the egg 50; and an evaluation unit 30 with at least one spectrometer 31 configured to receive the radiation transmitted by the egg 50 and to generate a spectrum of the radiation transmitted by the egg 50.and a data processing unit 33 configured to receive spectra generated by the spectrometer 31 and to store them as a reference spectrum or measurement spectrum; an identification unit 23 for generating identification data by means of which the spectra generated on an egg 50 can be uniquely assigned to the egg 50; and a classification unit 35; wherein the data processing unit 33 is configured to store spectra generated by the spectrometer 31 and associated identification data, and wherein the classification unit 35 is configured to determine the sex of the embryo based on at least one reference spectrum and at least one measurement spectrum. A corresponding method is further specified within the scope of the invention.

The invention relates to a method for preparing avian eggs for the purpose of characterizing an avian embryo contained within an egg. The method comprises the following steps carried out in a preparation station (4) after a period of incubation in a hatchery: - positioning an egg horizontally on a support, the longitudinal axis of the egg being parallel to the support on which it rests; - leaving the egg thus positioned to allow the embryo to migrate upwards along its longitudinal axis. Figure for the abstract: No figure

The present invention provides a transgenic female chicken comprising a recombinant Z sex chromosome comprising a nucleic acid sequence of an exogenous reporter gene encoding a red fluorescent protein (RFP) in at least one position of the Z sex chromosome. Methods of sex determination of chicken embryos in unhatched eggs are also provided.

Provided are compositions, feeds, rations, drinking water and methods of use thereof for inducing protective immunity against or preventing Clostridium perfringens infection and / or necrotic enteritis in poultry, optionally as single dose vaccines. The composition comprises live virulent Clostridium perfringens (CP) and a mucosal adjuvant, such as cholera toxin (CT), which is for administered at or after hatch by for example oral delivery. The composition can be used with an immunomodulatory composition administered in ovo before hatching or a boost composition. The composition can be administered as a single dose.

The present application belongs to the field of chicken egg gender identification, and particularly relates to a method for realizing chicken egg gender identification by fusing visible light images and hyperspectral data. The method comprises first-stage identification and second-stage identification. The first-stage identification adopts transmission light combined with YOLO intelligent algorithm to realize fertilized egg screening and initial gender classification by identifying the distribution of egg blood vessels. The second-stage identification adopts hyperspectral technology to collect the morphological and biochemical information of the egg for secondary gender identification. If the results of the two identifications are consistent, the female eggs continue to be incubated, and the male eggs are removed. If the results of the two identifications are inconsistent, the corresponding eggs are collected for separate incubation. The experimental results show that the two-stage method based on YOLO recognition and fusion of visible light images and hyperspectral data has an average accuracy of 98.9% in egg gender classification, which provides an effective solution for early chicken embryo gender identification.

The invention provides a method for constructing an anti-subtype J avian leukosis gene edited chicken based on adenine editor adenovirus and application of the anti-subtype J avian leukosis gene edited chicken. The method comprises the following steps: taking c.98Tgt of a chicken NHE1 gene; c, c, 100 Tgt; c, c.112 Tgt, c. 112 Tgt; c, c, 116Agt, c, 116Agt; the method comprises the following steps of: designing an adenine editor by taking a nucleotide sequence A and a nucleotide sequence G as targets, constructing plasmids of the adenine editor capable of simultaneously editing all the sites, further packaging an adenovirus containing the sequence, and injecting the adenovirus into an HH15-18 stage chicken embryo through a microinjection method to realize the NHE1 gene c.98Tgt in the chicken embryo; c, c, 100 Tgt; c, c.112 Tgt, c. 112 Tgt; c, c, 116Agt, c, 116Agt; the method comprises the following steps: carrying out site-directed mutagenesis on one or more of G.98Tgt and G. A chick embryo is hatched and developed to sexual maturity, and carrying out hybridization and breeding to obtain an NHE1 gene C, c, 100 Tgt; c, c.112 Tgt, c. 112 Tgt; c, c, 116Agt, c, 116Agt; g, gene edited chicken with one or more point mutations, wherein the gene edited chicken is proved to have the J subtype avian leukosis resisting character. The method has great application value in the fields of J subtype avian leukosis prevention and purification, gene editing chicken disease resistance model research and the like.

The present invention provides compositions comprising specific isolates of bifidobacterium bacteria. The composition may further comprise lactic acid bacteria. Methods of administering the compositions to a subject in ovo are also provided. The methods can be used, for example, to reduce the colonization of pathogens in the gastrointestinal tract of hatching chickens and thereby improve their growth performance.

The invention discloses an efficient Luhua chicken sex identification method based on SVM prediction, and relates to the technical field of chicken sex identification. The method at least comprises the following steps: S1, establishing an SVM prediction model; s2, data preparation: loading a data set into Python, carrying out necessary preprocessing, calling an info function in a pandas library of the Python to find that data with the number of 779 is a missing value, and removing the missing value; s3, required features are determined, and some features are increased or decreased differently under different categories, so that the category distinguishing capability of the model is further enhanced; s4, dividing a data set, and dividing the data set into a training set and a test set; according to the method, a set of SVM fuzzy model is constructed according to the egg shape index by researching different identification time, the Luhua chicken embryo structure and the model, a Luhua chicken sex identification technology model based on multiple indexes such as the egg weight and the egg shape index as main bodies is explored, and a theoretical reference is provided for early sex identification of embryos in production.

The invention belongs to the technical field of biology, and particularly relates to a circular RNA related to chicken intramuscular fat deposition, a ceRNA regulation network and application. According to the application disclosed by the invention, circSMARCD3 playing a key role in intramuscular fat deposition in a chicken embryonic period and a ceRNA regulation axis of the circSMARCD3 are screened and identified through a high-throughput sequencing technology, the cyclization stability of the circSMARCD3 is verified, the circSMARCD3 can be directly combined with miR-2131-5p, and the miR-2131-5p can directly target 3 'UTR regions of SORT1 and PDE4B genes and inhibit expression of the SORT1 and PDE4B genes, so that proliferation of preadipocytes in chicken embryonic period is inhibited, and the application of the circSMARCD3 in chicken embryonic period intramuscular fat deposition in chicken embryonic period intramuscular fat deposition in chicken embryonic period intramuscular fat deposition is promoted. The chicken feed additive can promote fat droplet aggregation to be remarkably increased, TG and GL contents to be remarkably increased, preadipocyte differentiation in chicken muscles is promoted, fat deposition is promoted, and the chicken quality is improved.

The present invention relates to transgenic avians and the eggs produced therefrom wherein the eggs comprise a genetic modification that facilitates in ovo gender sorting and a genetic modification that increases a production trait in the eggs or the avians produced therefrom. The present invention also relates to methods of identifying the gender of eggs before hatching and methods of sorting the eggs based on gender before hatching.