42 results about "Fat Droplet" patented technology

A method for counting somatic cells or fat droplets in milk on-line during milking by an automated or semi-automated milking system comprising the steps of: flowing milk as milked by the milking system through a measuring chamber (59); illuminating milk that flows through the measuring chamber; and recording multiple two-dimensional digital images of illuminated milk that flows through the measuring chamber, wherein the images are recorded through a lens system (49) to preferably obtain a spatial resolution better than about 5 microns in the images. Finally, a somatic cell or fat droplet count score of the milk is determined from the images by means of digital image processing, preferably including the use of neural networks.

In a culture method for adipocytes, seeded adipocytes are cultured while adhering the adipocytes to a culture bottom surface (14) and walls (12) positioned perpendicularly to the culture bottom surface (14), and mature adipocytes are obtained by generating spherical, enlarged fat droplets in cells without suspending the adipocytes in a culture medium. In the method, a culture vessel (10) is used in which the culture walls (12) are formed perpendicularly to the culture bottom surface (14). With cells or cell aggregates adhered in at least two places, namely the walls (12) and the culture bottom surface (14), spherical mature adipocytes having spherical enlarged fat droplets in cells are cultured. The adipocytes with a form close to in-vivo adipocytes are thus obtained.

The present invention concerns infant formula having a fat gradient which can be established by a sufficient amount of fat droplets with a diameter of 5-25 μm based on total weight of fat.

The invention discloses a method for fast and physically distinguishing the quality of mature ova of hucho taimen and relates to a physical evaluation method for the quality of the ova of hucho taimen. The method aims to solve the technical problems that in the existing multiplicative process of hucho taimen, the mature ova are evaluated by operators through intuitionistic senses from experience, the ova of hucho taimen cannot be evaluated at the early stage fast and accurately, consequently, the fertility rate and the hatchability decrease, and the membrane rupture offspring seed malformation rate and the floating offspring seed death rate increase. According to the method, the pubescent female parent fishes of hucho taimen are narcotized, the mature ova are extruded to a spawning basin containing an ovum buffer cleaning solution for trout from the abdominal cavities of the fish bodies, the average weight of the fish ova and the dispersion coefficient of the average weight, the average diameter of the fish ova and the dispersion coefficient of the average diameter and the average distribution density of fat droplets of the fish ova are measured, judgment is made on the basis of the physically-measured values to divide the fish ova into the superior grade, the medium grade and the inferior grade, the judgment can be completed in 1.5h, and the method has the advantage of being capable of being used for distinguishing the quality of the ova conveniently at the early stage. The method can be used in the field of aquaculture of hucho taimen.

The invention discloses application of FBXW5 and an FBXW5 inhibitor to preparation of a medicine for treating fatty liver and related diseases. An LO2 cell line, an FBXW5 overexpressed LO2 cell line,a wild type C57BL / 6 mouse primary hepatocyte, and a systemic FBXW5 gene knockout mouse primary hepatocyte are taken as experimental subjects, the function of the FBXW5 gene is studied through a palmitate in vitro stimulated and induced hepatocyte fatty degeneration model. The study finds that the area of a fat droplet of the FBXW5 overexpressed LO2 cell line is obviously increased compared with that of the LO2 cell line of the human normal hepatocyte under the same PA stimulation; and the degree of accumulation of the fat droplet in the FBXW5-KO mouse primary hepatocyte is obviously reduced compared with that of the WT mouse primary hepatocyte. The result shows that the fat accumulation is inhibited after the FBXW5 is inhibited. Therefore, the FBXW5 provides a target for the preparation ofthe medicine used for preventing, relieving and / or treating the fatty liver and the related diseases.

The invention provides the use of carrageenan polysaccharide in inhibiting the adipogenic transformation of mesenchymal stem cells, belonging to the technical field of stem cells. The present invention uses carrageenan polysaccharide to treat bone marrow mesenchymal stem cells, and finds that carrageenan polysaccharide can inhibit the mRNA expression and protein expression of genes PPARγ and C / EBPα related to adipogenic transformation, thereby inhibiting the process of adipogenic transformation of bone marrow mesenchymal stem cells The number of fat droplets generated, indicating that carrageenan can inhibit the adipogenic transformation of bone marrow mesenchymal stem cells.

A method for constructing a three-dimensional simulation model of fatty liver tissue. The three-dimensional simulation model is obtained through six steps. The three-dimensional simulation model construction method of fatty liver tissue can obtain three-dimensional simulation models under different liver fat contents. The three-dimensional simulation model construction method of fatty liver tissue can evaluate the three-dimensional distribution of fat droplets in liver cytoplasm with different fat contents on the scale of fat droplets.

The invention belongs to the technical field of medical equipment, and discloses a bone marrow collecting and filtering device and method; it is provided with: a syringe with a hole, a connecting hole, and a filter tube; it can realize the function of collecting bone marrow, and the head of the syringe with a hole is connected to a puncture needle, Bone marrow is extracted; compared with the traditional syringe, the perforated syringe has one more connection hole to connect to the outside, which can be connected to a filter tube for the bone marrow to be filtered and transported in a closed state; the first filter screen with a pore size of 300 microns can filter out The bone marrow granules composed of fibroblasts, as well as bone and skin debris, can prevent the catheter from being blocked; the second filter with a pore size of 100 microns can filter out fat droplets, hematopoietic island cell clusters, etc., and prevent embolism after transfusion into the patient's body. Complications; the flow rate meter can monitor the flow rate; the single-chip microcomputer can analyze the change of the flow rate and determine whether there are still impurities in the bone marrow fluid; the blood pump provides power to make the collected bone marrow flow in the device to speed up the efficiency of the filtration process.

Disclosed is a process for manufacturing a concentrate for making a premix for a frozen confection, wherein the concentrate is in the form of granules. The process comprises the steps of: (a) formingan oil-in-water emulsion wherein the fat droplets in the emulsion have an average particle size (D3,2) of less than 1 micron, the emulsion comprising fat in an amount of at least 65% by weight of theemulsion; (b) providing powder comprising saccharides, non-saccharide sweetener and / or protein; (c) combining, in a granulation step, the powder and the emulsion to form a mixture with a moisture content of less than 10% by weight of the mixture, wherein the emulsion binds the powder into granules; and (d) recovering the granules from step (c).

The invention discloses application of a nuclear factor chiB inhibition protein 1 combined with A20 to preparation of a medicine for treating fatty livers and related diseases and belongs to novel application of ABIN1 (A20binding Inhibitor of NF-KB Activation). According to the application, an L02 cell line, an ABIN1 over-expression L02 cell line, mouse primary hepatocyte cells and ABIN1 over-expression mouse primary hepatocyte cells are adopted as experiment targets, fatty liver cell models are induced through palmitic acid and oleic acid together, and oil red O dyeing results show that undercombined irritation of the palmitic acid and the oleic acid of a same concentration, the areas of red fat granules in ABIN1 over-expression L02 cells are remarkably reduced when being compared with those of L02 cells. The same oil red O dyeing result trend is also observed in mouse primary hepatocyte cells with over-expressed ABIN1, the result shows that ABIN1 over-expression has a remarkable inhibition function on lipid accumulation in palmitic acid and oleic acid induced fatty liver cells. Therefore, ABIN1 has functions of inhibiting liver lipid accumulation and protecting livers.

The invention discloses an application of OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 1 in preparation of medicines for treating fatty liver and related diseases. According to the invention, a normal human hepatocyte L02 cell line is taken as a research object, lentivirus can be used for constructing an OTUB1 knockout and overexpression vector system, and an OTUB1 gene knockout or overexpressed L02 cell is obtained. The function of the OTUB1 gene in fatty degeneration is researched through a palmitic acid (PA) and oleic acid (OA) combination-induced fatty liver cell model. The result displays that under same PA+OA stimulation, compared with the L02 cell normally expressed by the OTUB1 gene, the red fat droplets in the L02 cell knockouted by the OTUB1 gene are large and multiple; on the contrary, and the red fat droplets in the L02 cell overexpressed by the OTUB1 gene are small and less. The result shows that the OTUB1 gene can obviously inhibit liver lipid deposition and inhibit generation of fatty liver.

The invention discloses application of E3 ubiquitinligating enzyme TRIM32 for preparing drugs for treating fatty liver and related diseases. A human normal liver cell L01 cell line and a TRIM32 overexpression L02 cell line are adopted as experimental subjects, and the function of the TRIM32 gene is researched through a fatty liver cell model inducted by palmitic acid and oleic acid. A result showsthat under the stimulus of palmitic acid and oleic acid, the area of red fat droplets of TRIM32 overexpression L02 cells is remarkably increased compared with a control group, and it indicates that TRIM32 overexpression can worsen lipid accumulation generated by combined induction of palmitic acid and oleic acid; in other words, the TRIM2 gene can promote development and progression of the fattyliver and related diseases. Accordingly, TRIM32 provides a target for preparing drugs for preventing, relieving and / or treating the fatty liver and related diseases.