19 results about "Fat Droplet" patented technology

The invention discloses a chicken myoblast and chicken fat precursor cell co-culture synchronous differentiation method and application thereof, and belongs to the technical field of animal cell co-culture and differentiation. The method comprises the following steps: respectively carrying out in-vitro multiplication culture on chicken myoblasts and chicken fat precursor cells, carrying out mixed co-culture, inducing for at most two days by adopting horse serum with the volume percent of 0.5-2%, carrying out pre-differentiation, and continuously carrying out induced differentiation culture for at most one day by using an induced adipogenesis culture medium. According to the synchronous differentiation method for co-culture of the chicken myoblasts and the chicken fat precursor cells, a cell co-culture system is constructed, time sequence induced differentiation is achieved, and a final product with fat droplets distributed in the gaps of muscle bundles (the average distance is 20-50 microns) and capable of recarving natural chicken intermuscular fat textures is obtained. According to the method, the differentiation period is shortened to be within 3 days from 21 days (the efficiency is improved by 600%); the induction cost is reduced by 42.7%; the final product breaks through the sensory acceptance barrier through bionic structure recarving.

Disclosed is a process for manufacturing a concentrate for making a premix for a frozen confection, wherein the concentrate is in the form of granules. The process comprises the steps of: (a) forming an oil-in-water emulsion wherein the fat droplets in the emulsion have an average particle size (D3,2) of less than 1 micron, the emulsion comprising fat in an amount of at least 65% by weight of the emulsion; (b) providing powder comprising saccharides, non-saccharide sweetener and / or protein; (c) combining, in a granulation step, the powder and the emulsion to form a mixture with a moisture content of less than 10% by weight of the mixture, wherein the emulsion binds the powder into granules; and (d) recovering the granules from step (c).

The present invention relates to the field of nutritional compositions, methods of providing nutritional compositions and uses thereof. More particularly, the present invention relates to a ready-to-eat powdered infant formula or ready-to-eat powdered growth formula exhibiting desirable gastric digestion characteristics. There is provided a nutritional composition comprising casein, whey protein comprising alpha-lactalbumin (aLac) and beta-lactoglobulin (bLac), and protein-coated fat droplets wherein the composition has a protein content of from 5% to 20% (w / w) based on total solids; the bLac content is at least 3% (w / w) based on the total protein level, and the aLac + bLac content is at least 25% (w / w) based on the total protein level; the fat content is in the range of 10% to 50% (w / w) based on total solids; the fat droplets are coated with an average protein load of 2 to 10 mg / m2, and wherein the weight ratio of casein: (aLacc + bLac) on the fat droplets is at least 4 times the weight ratio of casein: (aLacc + bLac) in the total composition; the level of sphingomyelin per 100 g of the composition is between 70 mg and 200 mg; the level of phospholipids per 100 g of the composition is between 280 mg and 950 mg.

The invention belongs to the technical field of biology, and discloses application of pranlukast in preparation of drugs for preventing and / or treating fatty liver diseases, diabetes and obesity. The invention proves that pranlukast has the effect of relieving fat accumulation in vivo and in vitro; the pranlukast can obviously reduce lipid droplet accumulation in liver cells at an in-vitro cellular level, obviously reduce the weight of a metabolism-related fatty liver disease (MASLD) model mouse induced by a high-fat feed in vivo, and improve pathological abnormalities such as liver functions, glucose and lipid metabolism disorder, insulin resistance and liver fatty degeneration; it shows potential in preventing or treating diseases accompanied by the metabolic syndrome. Meanwhile, the invention further provides a medicine which contains pranlukast and is used for preventing and / or treating the diseases.

The invention pertains to a cheese analogue comprising a protein, a gelling agent, a fat and water, wherein the cheese analogue is an emulsion-filled gel and wherein the cheese analogue comprises fat droplets having a size exceeding 20 μm.

The invention relates to the field of medical preparations, in particular to application of ingenol in preparation of a medicine for treating liver injury of postmenopausal women. The ingenol intervenes in OVX to induce a postmenopausal obese mouse, and the ingenol is proved to be capable of remarkably reducing the weight and fat content of the mouse, reducing serum TC, TG and LDL-c level rise, improving hepatocyte swelling and reducing the number of liver fat droplets, and also capable of remarkably reducing serum AST and ALT level rise caused by OVX and relieving liver injury induced by OVX. Therefore, the ingenol can be used for effectively treating the liver injury of the postmenopausal women, and can be used for preparing a medicine for treating the liver injury of the postmenopausal women and a medicine for reducing the level of a liver injury marker in serum. The method can provide a new direction and strategy for future development and application of ingenol.

The invention relates to the technical field of medical instruments, in particular to a bone cement injection guiding device for vertebroplasty, which comprises a negative pressure suction guiding device and a bone cement injector, the negative pressure suction guiding device comprises a negative pressure tank, a guiding pipe and a connecting hose connecting the negative pressure tank and the guiding pipe, and a plurality of side holes used for forming a negative pressure area in the vertebral body are formed in the side wall of the guiding pipe. The bone cement injector comprises a working sleeve, a needle tube and a push rod, bone cement is contained in the needle tube, through the synergistic effect of integration of negative pressure suction and bone cement injection, the safety and effectiveness of an operation are remarkably improved, firstly, a controllable negative pressure environment is continuously created in a vertebral body before and in the bone cement injection process, and the bone cement is continuously injected into the vertebral body; and bone marrow, fat and blood stasis in the vertebral body can be effectively sucked and removed, and the risk of embolism caused by the fact that fat drops or blood stasis blocks enter a circulatory system due to pressure in the operation is greatly reduced.

The invention discloses a plant extract feed additive compound for increasing the fat droplet content of pork muscle cells, and belongs to the field of animal feed. The compound is prepared from the following components in parts by weight: 1 to 20 parts of carvacrol, 0.1 to 1.5 parts of thymol and 1 to 15 parts of cinnamyl aldehyde. By utilizing the synergistic effect of multiple components in the plant extract feed addition compound, the pork muscle cell lipid droplet content is obviously increased, and the tenderness, flavor and juiciness of pork are improved, so that the pork quality is improved. The invention provides a novel plant-derived compound for improving the pork quality, especially for improving the fat droplet content of pork muscle cells, and the compound can be safely and effectively applied to improving the pork quality.

The invention belongs to the technical field of biological medicines, and discloses a novel application of an epimedium aqueous extract, icariin and icaritin in improvement of senescence-related fat accumulation and redistribution. The invention constructs a nematode-mouse dual verification model: firstly, in a caenorhabditis elegans model, it is found that the active ingredients can significantly reduce fat droplet accumulation and triglyceride level in senescence nematode bodies, and especially under the concentration of 50 [mu] M, icariin can reduce the triglyceride content of 15-day-old senescence nematode to 37.58% of a control group. Furthermore, a naturally aged mouse is evaluated by utilizing a high-resolution Micro-CT (VivaCT 80) living body imaging technology, and three-dimensional reconstruction and quantitative analysis prove that the medicine can remarkably reduce the volume of visceral fat in the abdomen of the aged mouse, effectively improve the ratio of visceral fat to subcutaneous fat (VAT / SAT ratio) and reverse central obesity caused by aging. The raw materials are from natural plants, have no obvious toxic or side effect after long-term administration, can accurately improve fat space distribution of aging organisms, and have significant clinical application value.

The invention discloses a piperlongumine derivative shown in a structural general formula (I), a dialkyl amino coumarin fluorescent skeleton is introduced into a piperlongumine pharmacophore, and the piperlongumine derivative which has a lipid droplet targeting fluorescence labeling function and has anti-tumor and anti-aging activity is developed. The piperlongumine derivative disclosed by the invention can be used for lipid droplet imaging in tumor and senescence cells, is used for selectively acting on the tumor cells in vivo, inside and outside, and can selectively remove replicative senescence cells, recover senescence-related markers and gene expression, improve somatic function decline caused by senescence and improve the anti-aging effect of the piperlongumine derivative. And a new way is provided for treatment of clinical related diseases, drug development and preparation of in-vitro preparations or daily necessities.

The invention discloses a triphenylamine derivative as well as a preparation method and application thereof, and belongs to the technical field of fluorescent molecular probes. The invention solves the problems of poor stability, high cytotoxicity, low selectivity and sensitivity, poor specificity and the like of the current probe for lipid droplet detection. According to the method, 5-(4-(diphenylamine) phenyl) thiophene-2-formaldehyde and 6-hydroxy-3-coumarone are used for synthesizing a compound 1, the compound 1, 2-fluoro-5-nitrobenzoic acid, 4-dimethylaminopyridine and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride are used for synthesizing a triphenylamine derivative, and the derivative is used as a probe DPT-LDs for detecting lipid droplets. The DPT-LDs structure enhances the intramolecular charge transfer effect, has high selectivity, specificity and low cytotoxicity, has good responsiveness to low-polarity and high-viscosity environments, and successfully performs lipid droplet cell imaging in the liver of a mouse NAFLD model.

The present invention discloses a kind of excimer ratio fluorescent probe, preparation method and application that can be used for high-selectivity lysosomal polarity imaging in cells, and the fluorescent probe is recorded as Lyso-PRG, and only one-step reaction is required to prepare it, and its synthetic raw materials are easy to obtain, simple to operate, and easy to promote. The fluorescent probe is applied to prepare ratio-type lysosomal intracellular polarity change detection reagent or detection element. The fluorescent probe of the present invention pioneered the use of monomer-excimer imaging mechanism for the design of lysosomal polarity imaging probe, and then the obtained fluorescent probe shows extremely low background noise, ultra-high Stocks shift (130nm) and dual-color ratio fluorescence properties; and based on the basis of the fluorescent probe, by introducing quaternary amine positive charge structure, it can be avoided to be combined with lipid membrane and fat droplet, showing excellent lysosomal selectivity, and then in the sensitivity, resolution and accuracy of lysosomal polarity detection, it shows significant advantages, and it has broad application prospects in lysosomal polarity-related pathological research, drug efficacy evaluation and new drug research and development fields.

The invention relates to the technical field of preparation of bioactive substances, in particular to an eel extract rich in branched chain amino acid and application of the eel extract in anti-seasickness products. According to the method, the small-molecule oligopeptide is obtained through directional composite enzymolysis, degreasing and membrane separation technologies; directional composite enzymolysis is formed by compounding animal protease and neutral protease, whole wheat flour degreasing and green natural degreasing raw materials are adopted, a net structure of beta-glucan and araboxylan adsorbs grease through capillary action and surface tension, and oil-water interfacial tension can be reduced in an enzymatic hydrolysate liquid-phase system; the contact between fat droplets and the fiber surface is promoted, the adsorption efficiency and the bonding strength are enhanced, denaturation separation is synchronously carried out by enzyme deactivation and degreasing, and the production energy consumption is reduced; the obtained branched-chain amino acid-enriched eel oligopeptide, gamma-aminobutyric acid and the like are combined to prepare tabletted candies, so that the motion sickness effect of model seasickness mice can be remarkably improved, and the tabletted candies have an obvious dose-dependent effect, high-efficiency retention of active ingredients and function improvement.

The invention discloses a cryoprotectant for improving the freezing tolerance of cattle and sheep blastocysts and a freezing method, and the cryoprotectant for improving the freezing tolerance of the cattle and sheep blastocysts comprises cGMP and programmed freezing fluid. Immersing a blastocyst to be frozen in the cryoprotectant, placing the blastocyst on a heating table at 37 DEG C, and pretreating the blastocyst for 10 minutes; and freezing the embryo by adopting an embryo programmed freezing operation. According to the method, the blastocysts are soaked in the cryoprotectant containing cGMP for pretreatment, the content of lipid droplets in the blastocysts can be reduced, after the pretreated blastocysts are subjected to programmed cryopreservation treatment and unfreezing, the recovery rate and hatching rate of the cattle and sheep blastocysts are remarkably increased, better cellular morphology is shown, and the freezing tolerance of the blastocysts can be improved.

The present application relates to the technical field of induced transformation of cancer cells, and provides a method for inducing differentiation of cancer cells into adipocytes, which comprises the following steps: (1) culturing cancer cells in a basic medium, and when the confluence of the cells reaches 80-100%, inducing differentiation; (2) replacing the basic medium in step (1) with an induction differentiation medium 1, and culturing for 4-8 days; (3) replacing the induction differentiation medium 1 with an induction differentiation medium 2, and culturing for 1-4 days; (4) replacing with the basic medium, and culturing for 6-12 days, so that the cancer cells are successfully induced to differentiate into adipocytes; the method of the present application can transform malignant proliferative and metastatic cancer cells into mature adipocytes, and the adipocytes formed by differentiation contain fat droplets and highly express marker genes of mature adipocytes. The method of the present application can effectively inhibit the proliferation and metastasis of cancer cells, and provides a new idea and method for clinical cancer treatment, and has a good application prospect.

The invention relates to the field of in-vitro culture and induced differentiation of animal cells, in particular to a culture medium for rapid and synchronous myobiogenesis and adipogenesis serum-free co-induction of fish stem cells and an induction method. According to the method, a serum-free induction culture medium containing specific components is used, nutrition, metabolism and fatty acid delivery conditions required by muscle cell fusion and lipid droplet formation are met in the same induction system at the same time, the process complexity and batch-to-batch fluctuation caused by step-by-step induction are reduced, stable construction of the muscle-lipid composite tissue is achieved, and the method is suitable for large-scale popularization and application. Controllable supply and spectral pattern adjustment of key fatty acid components such as DHA and EPA are supported; synchronous differentiation is started when the cell fusion degree reaches the specified standard, and finally the fish-derived muscle-fat composite tissue with mature muscle fibers and fat droplets at the same time is obtained.

The application discloses a kind of dynamic oxidation protection system and method based on nitrogen medium circulation in fat powder production process, it is related to food and feed processing field, comprising the following steps: S1, nitrogen cooled to 5-15 ℃ is passed into fat powder spray cooling tower, and the dew point control target of the circulating nitrogen is set to ≤40 ℃, the temperature cooperates with dew point parameter, aims at realizing efficient cooling, preventing product moisture absorption and controlling system condensation simultaneously;S2, the cooling nitrogen is contacted with atomized fat droplets in tower, carries out cooling and dynamic oxygen isolation, and carries out fine powder and moisture;The dynamic oxidation protection system and method based on nitrogen medium circulation in fat powder production process, nitrogen is used as cooling medium to isolate oxygen from the source, cooperate closed loop circulation to maintain inert environment, so that the peroxide value of product is reduced by more than 60% compared with air cooling process, and the quality of product is realized leap.

The invention belongs to the technical field of fluorescent polymer synthesis, and provides a ratio-type fluorescent probe for detecting the polarity of a lipid droplet, and a preparation method and application of the ratio-type fluorescent probe. The structural formula of the fluorescent probe is shown in the specification. The compound can be prepared into a fat droplet tracer for detecting cells or organisms or a reagent for detecting polarity change of fat droplets. The probe is strong in water solubility and good in light stability, can be developed into a good biocompatibility lipid droplet tracing and polarity detection reagent, and has wide application value in medical diagnosis and clinic.

The invention discloses a CD73 gene and an application of a protein encoded by the CD73 gene in regulation and control of fat metabolism. A nucleotide sequence of the CD73 gene is shown as SEQ ID NO: 1. The expression quantity of the CD73 gene and the encoded protein thereof is in negative correlation with the fat content of the tissue. The CD73 gene and the encoding protein thereof influence the content of triglyceride and the number of lipid droplets and maintain the steady state of fat metabolism by regulating and controlling fat metabolism protein and fat metabolism signal channels. By increasing the expression quantity of the CD73 gene and the encoded protein thereof, fat degradation can be promoted, lipid droplets and triglyceride can be reduced, and fat deposition can be reduced. The CD73 gene and the expression quantity of the encoded protein thereof can be applied to regulation and control of pork fat deposition, improvement of carcass quality and improvement of production efficiency, and can also be used as a molecular target to develop related targeting technologies such as seed selection, identification and treatment. CD73 has a wide application prospect and a potential value in the aspect of fat metabolism regulation and control.