42 results about "Fat Droplet" patented technology

A method for counting somatic cells or fat droplets in milk on-line during milking by an automated or semi-automated milking system comprising the steps of: flowing milk as milked by the milking system through a measuring chamber (59); illuminating milk that flows through the measuring chamber; and recording multiple two-dimensional digital images of illuminated milk that flows through the measuring chamber, wherein the images are recorded through a lens system (49) to preferably obtain a spatial resolution better than about 5 microns in the images. Finally, a somatic cell or fat droplet count score of the milk is determined from the images by means of digital image processing, preferably including the use of neural networks.

A method and device for monitoring and controlling a pharmaceutical, chemical or food process, in which the biological activity and/or the bio-mass of micro-organisms in the process is monitored, and in which the state of the process is detected by monitoring viscosity and/or aggregate size and/or content of a dispersion of proteins, crystalline particles or fat droplets that are processed, respectively. The micro-organisms and/or bio-molecules produced in the process, and the proteins, crystals particles or fat droplets being processed are monitored by means of acoustic spectroscopy.

The invention discloses a method for fast and physically distinguishing the quality of mature ova of hucho taimen and relates to a physical evaluation method for the quality of the ova of hucho taimen. The method aims to solve the technical problems that in the existing multiplicative process of hucho taimen, the mature ova are evaluated by operators through intuitionistic senses from experience, the ova of hucho taimen cannot be evaluated at the early stage fast and accurately, consequently, the fertility rate and the hatchability decrease, and the membrane rupture offspring seed malformation rate and the floating offspring seed death rate increase. According to the method, the pubescent female parent fishes of hucho taimen are narcotized, the mature ova are extruded to a spawning basin containing an ovum buffer cleaning solution for trout from the abdominal cavities of the fish bodies, the average weight of the fish ova and the dispersion coefficient of the average weight, the average diameter of the fish ova and the dispersion coefficient of the average diameter and the average distribution density of fat droplets of the fish ova are measured, judgment is made on the basis of the physically-measured values to divide the fish ova into the superior grade, the medium grade and the inferior grade, the judgment can be completed in 1.5h, and the method has the advantage of being capable of being used for distinguishing the quality of the ova conveniently at the early stage. The method can be used in the field of aquaculture of hucho taimen.

The invention provides a use of carageen polysaccharide in inhibiting the mesenchymal stem cell adipogenic transformation, and belongs to the technical field of stem cells. The carageen polysaccharideis used to treat bone marrow mesenchymal stem cells, the result finds that the carageen polysaccharide can inhibit the expression of mRNA and protein of adipogenic transformation-related genes PPAR-gamma and C/EBP-alpha, and thereby inhibits a number of fat droplets produced in a bone marrow mesenchymal stem cell adipogenic transformation process, and indicates that the carageen polysaccharide can play a role in inhibiting the bone marrow mesenchymal stem cell adipogenic transformation.

The invention discloses an extraction method of adipose-derived stem cells. The extraction method comprises the following steps: acquiring adipose tissues, treating the adipose tissues with a surfactant, treating the adipose tissues with an adipose tissue protectant, performing inoculated culturing and the like. The used surfactant comprises the following components in parts by weight: 0.3 part ofhydroxyethyl starch 550, 1 part of lecithin. 0.1 part of polysorbate and 98.6 parts of a PBS solution. The used adipose tissue protectant comprises the following components in parts by weight: 0.2 part of trehalose, 0.05 part of reduced glutathione, 2 parts of human albumin, 1 part of histidine, 0.025 part of sorbic acid, 0.5 part of VC, and 96.2 parts of a carbohydrate and electrolyte injection.The extraction method can effectively solve the problems that in the extraction process of the adipose-derived stem cells, due to the existence of fat droplets, the cell attachment rate is low and the cell extraction rate is low.

The present invention concerns infant formula having a fat gradient which can be established by a sufficient amount of fat droplets with a diameter of 5-25 μm based on total weight of fat.

The invention discloses an application of a recombinant human cell globin on preparation of a fatty liver medicament. Through building an alcoholic liver rat model, subcutaneous injection is carried out for treatment according to the dose of 3mg/Kg of rhCYGB. An experiment shows that a recombinant human cell globin medicament is non-toxic; and in comparison with a model control group, an rhCYGB treatment group is remarkably improved, fat droplets are obviously reduced, and hepatic cords are levelly arranged. A reduced glutathione treatment group still has obvious fatty degeneration, and liver cells are lightly swollen. The rhCYGB has better efficacy in the treatment of an alcoholic fatty liver than the reduced glutathione. The recombinant human cell globin has the advantages of low toxicity, safety and effectiveness on fatty livers, and especially the alcoholic fatty liver.

The invention discloses application of FBXW5 and an FBXW5 inhibitor to preparation of a medicine for treating fatty liver and related diseases. An LO2 cell line, an FBXW5 overexpressed LO2 cell line,a wild type C57BL/6 mouse primary hepatocyte, and a systemic FBXW5 gene knockout mouse primary hepatocyte are taken as experimental subjects, the function of the FBXW5 gene is studied through a palmitate in vitro stimulated and induced hepatocyte fatty degeneration model. The study finds that the area of a fat droplet of the FBXW5 overexpressed LO2 cell line is obviously increased compared with that of the LO2 cell line of the human normal hepatocyte under the same PA stimulation; and the degree of accumulation of the fat droplet in the FBXW5-KO mouse primary hepatocyte is obviously reduced compared with that of the WT mouse primary hepatocyte. The result shows that the fat accumulation is inhibited after the FBXW5 is inhibited. Therefore, the FBXW5 provides a target for the preparation ofthe medicine used for preventing, relieving and/or treating the fatty liver and the related diseases.

The invention discloses a fatty liver tissue three-dimensional simulation model construction method. A three-dimensional simulation model is obtained through six steps. According to the fatty liver tissue three-dimensional simulation model construction method, three-dimensional simulation models under different liver fat contents can be obtained. The fatty liver tissue three-dimensional simulation model construction method can evaluate the three-dimensional distribution of the fat droplets in the cytoplasm with different fat contents according to the fat droplet scale.