112 results about "Horse serum" patented technology

The invention provides a mycoplasma anatis culture medium. The mycoplasma anatis culture medium is composed of a liquid culture medium and a solid culture medium. The liquid culture medium is prepared from deionized water, a 10% thallium acetate solution, PPLO broth, glucose, peptone, tryptone, 1% phenol red, a CMRL-1066 culture medium containing L-glutamine, inactivated horse serum, yeast leachate, penicillin, L-cysteine hydrochloride and nicotinamide adenine dinucleotide. Besides the situation that agar powder is added and phenol red is removed, other components of the solid culture medium are the same as those of the liquid culture medium. The culture medium is used for separating and purifying mycoplasma anatis, wherein a bacterial colony is separated from the solid culture medium, the separated bacterial colony is placed in the liquid culture medium to grow, then filtering, dilution and plate coating of liquid culture substances and purification of picked single colony multiplication are conducted, and the steps are repeated till purified mycoplasma anatis is obtained. The culture medium can promote growth of mycoplasma anatis, and the success rate of separation and purification of mycoplasma anatis is high.

The invention provides a recombinant pig FSH-CTP fusion protein. The fusion protein means that a beta subunit of pig FSH is directly or indirectly linked to a beta subunit carboxy terminal peptide CTPof human, primate or equine mammalian chorionic gonadotropin, and an alpha subunit of pig FSH is bound to the beta subunit of pig FSH through Van der Waals force. The fusion protein can be prepared by using a eukaryotic expression system on the basis of a genetic engineering technology. Compared with pig pituitary FSH, the pig FSH-CTP fusion protein provided by the invention has a longer half-life and a better drug effect, and can replace pig pituitary FSH and pregnant horse serum gonadotropin in the current market in the field of animal propagation.

The invention discloses a preparation method and application of cross-linked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca<2+> are crosslinked through a physical crosslinking method to form double-network hydrogel with high mechanical strength, and then the hydrogel is coated with heparin and collagen through a dipping coating method, so that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, the extracted primary muscle stem cells are inoculated onto the hydrogel and cultured for 24 hours in a growth culture medium (79% of DMEM, 10% of FBS and 1% of double antibodies); and then the extracted primary muscle stem cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 days. By utilizing the hydrogel, the absorption of the muscle stem cells on nutrient substances can be enhanced, and the growth of the muscle stem cells is facilitated. The dual-network hydrogel has the potential to be used as a scaffold for muscle stem cell growth of stem cell culture meat.

The invention discloses a mycoplasma bovis medium and a preparation method thereof. The mycoplasma bovis medium comprises a basic medium and an auxiliary medium through mixing and is prepared from Friis media premix, sodium pyruvate, a fresh yeast extract liquid, glucose, phenol red, deionized water, L-glutamine, L-cysteine, lactoalbumin hydrolysate, transferrin, insulin, penicillin and a small amount of horse serum. The medium not only can reduce the use amount of serum, but also remarkably increases the titer of living bacteria of mycoplasma bovis and lays a foundation for preparation of high quality vaccines of mycoplasma bovis.

The invention discloses a chemiluminescent detection kit for a bovine foot-and-mouth disease 3ABC antibody and belongs to the field of immunological detection. The chemiluminescent detection kit comprises a 96-well chemiluminescent immunoassay plate, an enzyme-labeled antibody, a serum diluent, a chemiluminescent substrate, a chemiluminescent enhancer and PBST washing liquid, wherein the 96-well chemiluminescent immunoassay plate is coated with 3ABC fusion protein, the enzyme-labeled antibody is a horseradish peroxidase labeled rabbit anti-bovine lgG antibody, the serum diluent comprises 0.01% of Tween-20, 1% of casein, 10% of horse serum and 1% of escherichia coli, the chemiluminescent substrate refers to a Luminol solution, and the chemiluminescent enhancer comprises IPP, H2O2 and Tween-20. Compared with commercially-available kit products, the chemiluminescent detection kit is high in sensitivity, higher in specificity and superior to other products in diagnosibility.

The invention discloses a mycoplasma ovipneumoniae low-serum medium, which consists of the following components: a PPLO broth, sodium pyruvate, phenol red, de-ionized water, lactose, tryptone, a fresh yeast extract, insulin, L-glutamine, L-cysteine, lactoalbumin hydrolysate, transferrin, penicillin and little horse serum; and the invention provides a preparation method of the mycoplasma ovipneumoniae low-serum medium. The mycoplasma ovipneumoniae medium provided by the invention has the following beneficial effects: a serum content is merely 5%, which is 1/4 of the serum content of a medium in the prior art, and the living bacterium titer of cultivated mycoplasma ovipneumoniae reaches 7*10<9> CCU/mL or above, which is significantly higher than that of the medium in the prior art. In comparison with the prior art, the mycoplasma ovipneumoniae low-serum medium provided by the invention has the characteristics of being low in serum content, rapid in growth and high in living bacterium titer.

The invention belongs to the technical field of veterinary biology, and relates to a mycoplasma hyopneumoniae selective separation medium and a preparation method thereof. The mycoplasma hyopneumoniae selective separation medium comprises the main components of lactoalbumin hydrolysate, yeast extract powder, MEM (minimum essential medium), Hank 's solution, 0.1% phenol red solution, healthy horse serum, penicillin, deionized water, and specific growth inhibition serum. The mycoplasma hyopneumoniae selective separation medium can be used for the rapid separation and identification of mycoplasma hyopneumoniae, the preparation method is simple and feasible, experimental results are reliable, the separation time is shortened to 103h-122h, the time is shortened to 17%-23%, the separation success rate is as high as 75% above, and is more than 10 times of the separation success rate of mediums in the prior art.

The invention discloses a method for purifying myoblast derived from bovine fetus skeletal muscle tissue. The method is characterized in that the material used by the method is the longissimus dorsi muscle tissue of a 3-month-old bovine fetus, and reagents used by the method are horse serum, anti-fast muscle myosin heavy chain antibodies, collagenase, fetal bovine serum, a low-sugar Dulbecco modified Eagle medium, a cell-sorting buffer solution and anti-platelet derived growth factor receptor alpha antibodies.

The invention discloses an equine embryo-flushing solution as well as a preparation method and applications of the solution. The embryo-flushing solution contains sodium lactate mixed liquor, penicillin, streptomycin and one, two or three of bovine serum albumin, neonatal bovine serum and inactivated horse serum. The preparation method comprises the following steps: placing the sodium lactate mixed liquor in thermostat with the temperature of 37 DEG C for 0.5-6 hours for standby; and adding the penicillin, streptomycin and one, two or three of bovine serum albumin, neonatal bovine serum and inactivated horse serum in the sodium lactate mixed liquor according to the component contents to obtain the embryo-flushing solution. The preparation method is suitable for the non-surgical embryo transfer technology of horses, cattle and donkeys. The equine embryo-flushing solution has the following beneficial effects that the solution has the advantages that raw materials are available, cost is low, preparation method is simple and use is convenient, is very suitable for the equine large-scale non-surgical embryo transfer and is beneficial to the popularization and application of the embryo transfer technology.

This invention relates to a preparation method of multivalent anti-snake venom chicken yolk antibody. It includes following steps:(1) collect different kinds of snake venom antigen; (2) preparation of chicken ovum of anti-snake venom chicken yolk antibody, and infuse snake venom to chicken for immunization, after 20 to 4 weeks continuously collect egg for 6 to 10 mouths.(3) extract multivalent anti-snake venom chicken yolk antibody. This product can replace horse serum for injection, and be able to developed as oral using drug which has extensive application prospects in prevention of snake bite.

The invention provides a method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum. Under the sterile and anaerobic condition, anaerobic bacteria are used, a mixture consisting of peptone, beef extracts, yeast extracts, Na2HPO4, L-cysteine, L-cysteine hydrochloride monohydrate, diazoresorcinal indicators and distilled water is used as a culture medium, glucose is used as a fermentation substrate, a batch fermentation method is adopted for preparing the hydrogen, and the special anaerobic bacteria are clostridium pasteurianum JCM1408T. The culture medium consists of 10.0 to 15.0g of peptone, 2.0 to 3.0g of beef extracts, 5.0 to 10.0g of yeast extracts, 3.0 to 4.0g of Na2HPO4, 0.2 to 0.5g of L-cysteine, 0.2 to 0.5g of L-cysteine hydrochloride monohydrate, 1ml of 0.2 percent wt diazoresorcinal indicators, 40 to 60ml of horse serum and 1L of distilled water. The hydrogen can be continuously prepared.

The invention belongs to the technical field of genetic engineering and relates to a cell clone with MyoG (myogenin) gene knock-in and MSTN (myostatin) gene knock-out prepared by Crispr/Cas9 technique. The cell clone is characterized in that after being isolated and purified, the cell clone may be induced by 2% horse serum to differentiate into muscle fibers; skeleton satellite cells herein can merge into myotubes; an MSTN gene knock-out vector target is connected successfully, with no base mutations occurring; experimental group cells can differentiate into a certain quantity of myotubes; it is indicated that after MyoG gene is interfered, the cells still can differentiate into myotubes, while total myotube fusion rate is reduced and the form and quantity are significantly differed from those of a control group.

The invention discloses a mycoplasma capricolum subsp. Capripneumoniae low-serum high efficiency medium. The mycoplasma capricolum subsp. Capripneumoniae low-serum high efficiency medium comprises the following components of PPLO broth, sodium pyruvate, lactose, tryptone, fresh yeast extract, L-glutamine, L-cysteine, lactoalbumin hydrolysate, horse serum, penicillin, phenol red, and deionized water; and the invention also provides a preparation method. The mycoplasma capricolum subsp. Capripneumoniae low-serum high efficiency medium has the beneficial effect that the serum content of the mycoplasma capricolum subsp. Capripneumoniae low-serum high efficiency medium is only 5%, which is 1/5-1/4 of the serum amount of the mediums in the prior art; in addition, the prepared semi-finished product bacteria liquid titer can reach 1010 CCU/ml, which is obviously higher than that of the medium in the prior art. The low-serum high efficiency medium used for culturing the mycoplasma capricolum subsp. Capripneumoniae is in favor of mitigating allergic stress reaction of heterogenous serum on goat, increases the biological safety, increases the live bacteria titer, and reduces the production cost.

The invention relates to an aged rat brain vascular endothelial cell culture fluid. On the basis of each 100 ml of DMEM (Dulbecco Modified Eagle Medium) culture medium, components including plasma derived serum (PDS), penicillin-streptomycin, L-glutamine, gentamicin, heparin, an alkaline fiber growth factor, an endothelial cell growth replenishing factor, vitamin C, a vascular endothelial growth factor, an insulin-like growth factor-1 and an endothelium growth factor are added. According to the aged rat brain vascular endothelial cell culture fluid, the PDS is adopted for replacing FBS (Fetal Bovine Serum), beef calf serum or horse serum in the traditional culture fluid to ensure that the purity of brain vascular endothelial cells is increased by 30 percent; and the vitamin C and puromycin are added to ensure that the brain vascular endothelial cells grow more rapidly and purely.

The invention discloses a culture medium for in-vitro culture of osteoblasts. The culture medium is characterized by being prepared from 10.0-15.0 g/L of a basal culture medium, 100-300 mg/L of tremella polysaccharide, 8-10 mL of serum, a proper amount of sodium bicarbonate for regulating the pH value to be 7.2-7.6, and the balance water added for achieving the constant volume of 1 L, wherein the basal culture medium is a DMEM or an alpha-MEM or RPMI-1640 or F12 or DMEM/F12, the purity of the tremella polysaccharide is larger than 75%, and the serum is fetal calf serum or calf serum or human serum or horse serum or sheep serum. The culture medium is used for in-vitro culture of osteoblasts. The culture medium has the advantages that growth of osteoblasts can be accelerated, and the use amount of the serum can be reduced.

The invention discloses a lower-serum and efficient culture medium of mycoplasma ovipneumoniae. The lower-serum and efficient culture medium is prepared from the following components: MEM, sodium pyruvate, glucose, Hank's liquid, fresh yeast leaching liquid, L-glutamine, L-cysteine, lactalbumin hydrolysate, calf thymus DNA (Deoxyribonucleic Acid), insulin, transferrin, penicillin, horse serum, phenol red and de-ionized water; the invention provides a preparation method of the lower-serum and efficient culture medium. The lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, has the beneficial effects that the content of the serum is only 5 percent and is 1/4 of the content of the serum in a culture medium in the prior art; the titer of mycoplasma ovipneumoniae semi-finished-product bacterium liquid prepared by the method reaches 10<10>CCU/ml and is obviously higher than that of the culture medium in the prior art. According to the lower-serum and efficient culture medium of the mycoplasma ovipneumoniae, disclosed by the invention, the sensitive stress response to a goat body, caused by heterologous serum, is alleviated, and the biosafety is improved; the titer of bacterium liquid of living bacteria is also improved and the production cost is reduced.

The invention relates to a marine fish embryo cell culture technology, in particular to an establishing method of a myosatellite cell system of bastard halibut during an embryonic period. The establishing method comprises the following steps: carrying out in-vitro separation on an embryo during a bastard halibut kirschner capsule formation period, removing an egg membrane, dispersing cells, and carrying out primary culture and secondary culture, thus establishing the myosatellite cell system during the embryonic period. The form of the myosatellite cell system, established by the invention, ofthe bastard halibut is fusiform or spindle-shaped mononuclear cells; myofiber can be differentiated to form by culturing for 5 days or above within a single generation or carrying out induction of anexogenous factor (horse serum). The myosatellite cell system is capable of supplying a large amount of myosatellite cells, and GFD (Green Fluorescent Protein) transfection and cynoglossus semilaevisspleen and kidney necrosis virus challenge assay detection verify that the myosatellite cells can be normally transfected or infected, so that the myosatellite cell system not only can be directly used for researching functional genes related to muscle growth and development of fishes and providing research materials for exploring a molecular mechanism for induction, proliferation and differentiation of muscle cells of the fishes, but also is capable of providing a platform for improved research and application of muscle characters in bastard halibut breeding.

The invention relates to the technical field of exosome extraction technology, and provides an extraction process of extracellular exosomes based on differential centrifugation. The culture for culturing HEK-293 cells is preferably a high-glucose DMEM culture medium, the problem that the adhesion strength of the HEK-293 cells is relatively small during the culture process can be effectively solved, so that the HEK-293 cells in the culture medium are produced at the same location, and the HEK-293 cells are isolated conveniently; in the culture process of the HEK-293 cells, 10% of horse serum isadded to the DMEM culture medium, a similar biological internal environment can be provided for cells cultured in vitro, the horse serum further contains several animal hormones and enzymes, and thedevelopment and growth of the HEK-293 cells can be promoted; and step S1, step S2 and step S3 are carried out on the HEK-293 cells before the differential centrifugation is performed, a supernatant extracted from the cells can be concentrated, the supernatant is collected after concentration, the purity of the exosomes in an extract can be improved to a certain extent, and the extraction effect isbetter.

The invention discloses a culture medium for separation and purification of mycoplasma bovirhinis. Every 1000ml of culture medium comprises, by weight 10.5g of PPLO (pleuropneumonia-like organism) broth, 8,0g of cerebrocardiac leach liquor, 4.0g of sodium chloride, 0.05g of magnesium sulfate, 0.2g of potassium chloride, 0,09g of calcium chloride, 0.024g of disodium hydrogen phosphate, 0,03g of monopotassium phosphate, 5.0g of glucose, 5.0g of lactoalbumin hydrolysate, 60ml of 25% of fresh yeast leach liquor, 100ml of aseptic swine serum, 100ml of aseptic horse serum, 100mg of ampicillin and 1ml of 10% of thallium acetate. The invention further provides a preparation method and application of the culture medium. Compared with the prior art, the culture medium for separation and purification of the mycoplasma bovirhinis, the preparation method and the application of the culture medium have the advantages of high growing speed and titer and high mycoplasma bovirhinis separation success rate.

The invention opened a method of exacting IgG. The method is to put the serum into the ABS then to adjust the pH by the NaOH, next to add the ethyl and PBS, last to add the (NH4)2SO4. The purity of the product is enhanced from 65% to 97%. The method can be used for the serum of rabbit, horse, sheep, chicken and mouse.

Provided is a ureaplasma urealyticum/mycoplasma hominis combined rapid culture and drug sensitivity detection kit. The kit can complete detection in 8-24 h, and meanwhile, drug-resistant detection of various antibiotics can be performed. The combined rapid culture kit is mainly composed of pork stomach digestive juice or lung digestive juice, a beef extracting solution or a beef heart extracting solution, cattle serum or horse serum or other animal serum, sodium chloride, peptone, yeast powder, urea, L-arginine, phenol red indicator or cresol red indicator, and penicillin or other antibiotics. The drug detection kit can detect doxycycline hyclate, roxithromycin, acetylspiramycin, gatifloxacin, clarithromycin, azithromycin, clindamycin, erythromycin, kitasamycin, ofloxacin, levofloxacin hydrochloride, sparfloxacin, ciprofloxacin, doxycycline, erythromycin cydocarbonate and various other antibiotics. By means of the detection kit, not only is the mycoplasma detection time shortened, but also drug-resistant detection can be performed at the same time so that clinical medication can be guided. The detection kit has the beneficial effects of being high in sensitivity, high in specificity, high in detection rate, easy and convenient to operate and the like.

The invention discloses a haemophilus parasuis culture medium. The haemophilus parasuis culture medium consists of a basic culture solution and an auxiliary material, the auxiliary material is a mixture of nicotinamide adenine dinucleotide, a phenol red indicator, linkkinin and horse serum; each L of the basic culture solution comprises the following components: 15.0 to 17.0 g of tryptone; 3.0 to 5.0 g of plant peptone; 4.0 to 5.5 g of sodium chloride; 2.0 to 3.5 g of dipotassium phosphate; 2.0 to 3.5 g of glucose; and the balance of water. The haemophilus parasuis culture medium is rich in nutrient components, very suitable for rapid growth of haemophilus parasuis and capable of improving the separation rate of the haemophilus parasuis in a polluted material, phenol red is added into the culture medium to serve as an indicator, on one hand, growth of the haemophilus parasuis cannot be affected, on the other hand, change of acidity and alkalinity can be indicated, in the growth process of the haemophilus parasuis, glucose is fermented to produce acid, alkali in a culture solution can be neutralized, the color of phenol red is changed from red to yellow, and the growth condition of the bacteria can be judged according to the change of the color of the phenol red.

The invention discloses a nutrient solution for detecting bacterial vaginosis. Each 100ml of nutrient solution contains the following raw materials: 0.1-1g of beef heart powder, 0.5-5g of tryptone, 0.1-2g of glucose, 0.1-1g of sodium chloride, 0.1-1g of bromocresol purple sodium, 5-20ml of horse serum, 0.0001-0.001g of neomycin sulfate and 0.0001-0.001g of nystatin. The invention further discloses a kit for detecting bacterial vaginosis and a detection method. The detection kit is stable in quality; the detection method is simple in operation and accurate in result. The kit has the functions of target cultivation and simultaneous detection of the sensitivity of a drug, the detection method is completed in one step within 6-24h, the success rate is high, the drug sensitivity result is reliable, clinical accurate diagnosis of the bacterial vaginosis and the drug sensitivity test can be met, a reference foundation is provided for a clinician in timely accurate diagnosis and reasonable use of the drug, and clinical abuse of antibiotics is avoided.

The invention discloses a normal temperature preserving liquid for equine animal embryos, and a preparation method and application thereof. The normal temperature preserving liquid includes a sodium lactate mixed solution, penicillin, streptomycin, and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum. The preparation method includes the steps of: placing the sodium lactate mixed solution in a thermostat at 37 DEG C for 0.5-6 h for standby; and adding penicillin, streptomycin and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum into the sodium lactate mixed solution in accordance with the component contents, so as to obtain the normal temperature preserving liquid for embryos. The preserving liquid is suitable for non-surgical method for embryo transplantation of horses, cattle and donkeys. The invention has the beneficial effects of convenience for material acquisition, low price, simple preparation and convenience for usage, is very suitable for scale production of non-surgical method for embryo transplantation of equine animals, and is favorable for popularization and application of embryo transplantation technology.

The invention discloses a method for inducing estrus and ovulation of a female cat, and belongs to the technical field of animal artificial propagation. The invention provides the method for inducingestrus synchronization and superovulation of the female cat in order to solve the problem that estrus and ovulation are difficult to synchronize in artificial propagation of the female cat. The methodspecifically comprises the following steps: continuously feeding adult healthy female cats with altrenogest with a dosage of 0.01-20mg/cat/day for 10-20 days, stopping feeding the altrenogest for 2 to 3 days, injecting 10-1000IU of pregnant mare serum hormone into the muscle of the female cat, and intramuscularly injecting 10-1000 IU of human chorionic gonadotropin after 72-96 hours, so that thefemale cat can perform estrus synchronization and superovulation within a preset time. The key technical difficulty of artificial breeding of the domestic cat or the feline is solved.