16 results about "Horse serum" patented technology

The invention discloses a chicken myoblast and chicken fat precursor cell co-culture synchronous differentiation method and application thereof, and belongs to the technical field of animal cell co-culture and differentiation. The method comprises the following steps: respectively carrying out in-vitro multiplication culture on chicken myoblasts and chicken fat precursor cells, carrying out mixed co-culture, inducing for at most two days by adopting horse serum with the volume percent of 0.5-2%, carrying out pre-differentiation, and continuously carrying out induced differentiation culture for at most one day by using an induced adipogenesis culture medium. According to the synchronous differentiation method for co-culture of the chicken myoblasts and the chicken fat precursor cells, a cell co-culture system is constructed, time sequence induced differentiation is achieved, and a final product with fat droplets distributed in the gaps of muscle bundles (the average distance is 20-50 microns) and capable of recarving natural chicken intermuscular fat textures is obtained. According to the method, the differentiation period is shortened to be within 3 days from 21 days (the efficiency is improved by 600%); the induction cost is reduced by 42.7%; the final product breaks through the sensory acceptance barrier through bionic structure recarving.

The invention discloses a timed insemination method for improving the pregnancy rate and the lambing rate of sheep, and belongs to the technical field of livestock breeding. In order to solve the problem that the pregnancy rate of existing sheep is unstable during regular insemination, the method comprises the steps that a progesterone sponge suppository is embedded in the vagina of a healthy and reproductive ewe on the 0th day, and meanwhile 25 g of luteinizing hormone is injected in an intramuscular mode to release hormone A3; removing the thrombus on the 12th day, and performing intramuscular injection of 400IU of pregnant mare serum gonadotropin at the same time; and after the suppository is removed, insemination is carried out on the cervix orifice of the ewe with ram semen diluted at the ratio of 1: (5-8) in 52h and 64h respectively. According to the method, ovarian activities of the ewes are regulated and controlled, the estrus synchronism and the ovulation rate are improved, tests show that the pregnancy rate reaches 81.6%, the lambing rate is 1.14, compared with a conventional method, the pregnancy rate and the lambing rate of the sheep are remarkably improved, and development of the sheep industry is promoted.

The invention discloses a mutant streptococcus protein G. The invention also discloses a polynucleotide, an expression vector, a host cell and a fusion protein. The invention also discloses an adsorption material, wherein the mutant streptococcus protein G or the fusion protein is fixed on a carrier to obtain the adsorption material. The invention also discloses application of the mutant streptococcus protein G, the fusion protein and the adsorption material in separation and purification of F (ab ') 2 fragments and derivatives thereof as chromatographic column packing. The invention also discloses a method for separating and purifying the F (ab ') 2 fragment, which comprises the following steps: carrying out column chromatography on a sample containing the F (ab') 2 fragment by using the adsorption material as a chromatographic column filler to obtain the F (ab ') 2 fragment. The mutant streptococcus protein G can specifically target an F (ab ') 2 fragment, is not combined with the performance of an Fc fragment, and can quickly and efficiently purify the F (ab') 2 fragment from horse serum.

The present application relates to the biomedical field, disclose a kind of method for removing vitamin D in horse serum, comprising: material screening;25(OH)D in horse serum is physically adsorbed using selected material;After physical adsorption, horse serum is centrifuged;After centrifugation, horse serum is filtered using filter membrane;Selected material is dextran treatment modified activated carbon, dextran treatment modified activated carbon is repeatedly obtained by immersing treatment of modified activated carbon in saturated dextran solution;The mass ratio of dextran and modified activated carbon is 2-10:1;Modified activated carbon is obtained by bonding hydrophobic activated carbon using nitric acid pretreatment activated carbon and diphenyl dimethoxysilane, then using phytic acid to treat hydrophobic activated carbon and calcining to make, the method is efficient, low cost, can remove 25(OH)D in horse serum in scale, it also has high specificity, only remove 25(OH)D, retain total protein in horse serum;Simple operation, without complex equipment.

The invention belongs to the technical field of biological medicines, and particularly relates to a neisseria gonorrhoeae chromogenic culture medium. According to the chromogenic culture medium, a supplement and a composite bacteriostatic agent are additionally added into a gonococcus culture medium, and the supplement is prepared from 50-200 ml / L of horse serum, 1-20 mg / L of hemin chloride, 25-250 mg / L of L-proline beta-naphthylamide hydrochloride, 500-5000 mg / L of 1, 3, 4-trimethyl-1, 3-pentanediol monoisobutyrate, 10-20 mg / L of sodium chloride, 10-20 mg / L of sodium chloride, 10-20 mg / L of sodium chloride, 10-20 mg / L of sodium chloride and 10-20 mg / L of sodium chloride. The invention relates to a neisseria gonorrhoeae culture medium, which is characterized in that neisseria gonorrhoeae in a sample can be directly separated and identified by adopting the culture medium, and a separated bacterial colony can be directly used for a drug sensitive test. The neisseria gonorrhoeae can be directly separated and identified by adopting the neisseria gonorrhoeae culture medium for the drug sensitive test of neisseria gonorrhoeae in the sample.

The invention relates to the field of biomedicine, and discloses a method for removing vitamin D in horse serum, which comprises the following steps: screening materials; physically adsorbing 25 (OH) D in horse serum by using the selected material; centrifuging the horse serum subjected to physical adsorption; filtering the centrifuged horse serum by using a filter membrane; the selected material is dextran-treated modified activated carbon, and the dextran-treated modified activated carbon is obtained by repeatedly placing modified activated carbon in a saturated dextran solution for dipping treatment. The mass ratio of the glucan to the modified activated carbon is (2-10): 1; the modified activated carbon is prepared by the following steps: pretreating activated carbon with nitric acid, bonding the pretreated activated carbon with diphenyldimethoxysilane to obtain hydrophobic activated carbon, treating the hydrophobic activated carbon with phytic acid, and roasting at high temperature, the method is efficient, low in cost and capable of removing 25 (OH) D in horse serum on a large scale, meanwhile, the method has high specificity, only 25 (OH) D is removed, and total protein in the horse serum is retained; the operation is simple and complicated equipment is not needed.

The invention belongs to the technical field of vaccine preparation, and particularly relates to a Brucella bovis S19 strain outer membrane vesicle, a preparation method thereof and a vaccine.The preparation method comprises the following steps that S1, a Brucella bovis S19 strain is taken and inoculated into a TSB culture medium, horse serum with the mass percentage being 3%-5% is added, and all bacterial liquid is inoculated into a new TSB culture medium to serve as first-level working seeds for production; s2, inoculating a culture medium with the first-stage working seeds for production, adding peptone water, washing off the lawns, and inoculating the lawns into a seed tank filled with a TSB culture medium to serve as second-stage working seeds for production; s3, adding a defoaming agent into the culture medium, adding secondary working seed bacterial liquid for production, and harvesting bacterial liquid; s4, centrifuging the cultured bacterial liquid at 2-8 DEG C, taking the supernatant, passing the supernatant through a 0.45 m filter membrane, and then carrying out ultracentrifugation to obtain a precipitate; and S5, re-suspending the precipitate with a PBS buffer solution, and filtering with a 0.22 [mu] m filter membrane to obtain the Brucella abortus S19 strain outer membrane vesicles.

The invention relates to a centralized collection method and a centralized collection device for horse serum, and belongs to the technical field of tools or methods for veterinarians. The collecting device comprises a collecting platform assembly and fixing frames, and is characterized in that the collecting platform assembly comprises sleepers, a fixed track, a roller assembly, a gear motor, a rotating track, a turntable platform and a fence, a plurality of serum collecting stations are arranged on the outer ring of the upper surface of the turntable platform, and each station is provided with one fixing frame for fixing horses; a control room is arranged in the center of the turntable platform, a PLC (Programmable Logic Controller) is arranged in the control room, a horse entering channel and a horse exiting channel are arranged outside the turntable platform, and an intelligent horse identification device, a blocking door and a horse outlet matched with the blocking door are arranged on the horse entering channel. The horse serum collecting device has the advantages that whether horses meet collecting requirements or not can be recognized, dynamic circulating collecting is achieved, the number of collected horses is large, occupied space is small, horse serum collecting efficiency is high, quality is high, labor intensity of workers is low, and safety is high.

The present application relates to the field of biological products and veterinary medicine, and discloses a method for extracting and purifying blood gonadotropin, which comprises the following steps: 1) adjusting the pH of pregnant mare serum to 4.5-5.0 by using metaphosphoric acid, and performing solid-liquid separation to collect supernatant; 2) precipitating the supernatant by using a first precipitant, and collecting filtrate; 3) precipitating the filtrate by using a second precipitant, collecting precipitate, and drying the precipitate to obtain crude blood gonadotropin; 4) dissolving the crude blood gonadotropin, and sequentially performing first ultrafiltration, affinity chromatography, second ultrafiltration, and cation exchange chromatography to obtain crude blood gonadotropin filtrate; precipitating the crude blood gonadotropin filtrate by using a third precipitant, collecting precipitate, and drying the precipitate to obtain pure blood gonadotropin. The purity of the blood gonadotropin prepared by the extraction and purification method can reach more than 99.9%, the total yield can reach 88.9%, and the titer can reach 11000 IU / mg.

The invention relates to a culture medium for culturing human mycoplasma pneumoniae and a culture method, and belongs to the technical field of microorganisms, the culture medium for culturing the human mycoplasma pneumoniae comprises a mycoplasma culture medium and a growth promoting additive, the growth promoting additive comprises horse serum or fetal calf serum, cholesterol, penicillin sodium and an HEPES buffer solution, according to the culture medium for culturing the human mycoplasma pneumoniae, various components are simple and easy to obtain, the preparation method of the culture medium is greatly simplified, and the efficiency is improved; according to the culture method of the human mycoplasma pneumoniae, the culture mode is changed from a static state to a dynamic state, so that the culture efficiency of the human mycoplasma pneumoniae is improved, and meanwhile, the thallus yield of the human mycoplasma pneumoniae is also improved; the culture method for preparing the human mycoplasma pneumoniae is short in period, low in cost, high in culture viable organism density and high in yield, and is suitable for large-scale culture of the human mycoplasma pneumoniae.

The invention belongs to the field of microbial culture media. The invention provides a mycoplasma synoviae culture medium and a preparation method thereof, the culture medium comprises oat hydrolysate, brain heart extract powder, yeast extract powder, beef extract powder and specific nutritional factors, and the specific nutritional factors comprise horse serum, vitamins, arginine and glucose. The invention also provides a preparation method of the culture medium. The culture medium contains basic nutrient substances and specific nutritional factors required by growth of the mycoplasma synoviae, under the synergistic effect of the components, the viable bacteria titer is remarkably improved, high titer is achieved, the serum dosage is remarkably small, the influence of exogenous factors in serum on mycoplasma products is reduced, and the culture medium is more suitable for production.

This invention relates to the field of animal reproductive regulation technology, and discloses a twinning regulator for sika deer males that synchronize estrus and its application method. The regulator is a homogeneous and transparent sol system prepared from trehalose, pituitary follicle-stimulating hormone (FSH), medium- and low molecular weight sodium hyaluronate, low molecular weight dextran sulfate, zinc gluconate, and physiological saline. This invention utilizes sodium hyaluronate of a specific molecular weight and dextran sulfate to construct a polysaccharide sol network, combined with the cross-linking effect of zinc gluconate and the physicochemical stabilizing effect of trehalose, to encapsulate and slowly release FSH. The application method includes implanting and removing a vaginal silicone plug, combined with the injection of pregnant mare serum gonadotropin (PMSG) and the regulator, followed by natural mating. This invention prolongs the half-life of exogenous hormones in the animal, avoids drastic fluctuations in blood drug concentration, and improves the stability of twinning rates in sika deer.

The invention belongs to the field of veterinary drugs, and discloses a novel veterinary drug for promoting ovulation and pregnancy rate of female livestock and a preparation method thereof. The veterinary drug is prepared from gonadorelin, human chorionic gonadotropin, pregnant mare serum gonadotropin, vitamin A palmitate, vitamin E acetate, sodium selenite, zinc sulfate heptahydrate, herba epimedii powder, radix angelicae sinensis powder and corn starch. The preparation method comprises the key steps of performing cryogenic crushing on the composite traditional Chinese medicine powder, adding the fat-soluble vitamins through melt spraying, performing gradient mixing on hormones, performing vibrated fluidized bed drying and the like. Through the synergistic effect of hormones, vitamins, trace elements and traditional Chinese medicines, the problems of single ovulation promoting effect, limited pregnancy rate improvement and poor preparation stability in the prior art are effectively solved. Tests prove that the veterinary drug can remarkably improve the ovulation rate and pregnancy rate of cows, sows and ewes, and the veterinary drug is good in product uniformity, high in stability and convenient to use and has good clinical application prospects.

The application provides a method for artificial insemination of sheep, which synchronously induces estrus of ewes by implanting a vaginal sponge, promotes follicular development by combined injection of pregnant mare serum when the sponge is removed, and ensures accurate mating time by combining estrus testing and timed insemination scheme; after training by supplemental feeding, semen is collected by using a false vagina with a temperature of 38-40 DEG C, and the motility of the diluted semen is maintained above 0.6; at least one of the two ways of conventional insemination or laparoscopic insemination is selected for insemination; the laparoscopic insemination establishes an operation channel by puncturing the base of the udder of the ewe, and the semen is accurately injected into the first third of the large bend of the uterine horn on both sides under the guidance of the laparoscope, so that the pregnancy rate can reach above 90%; and the pregnancy is checked by using B-ultrasound 25-30 days after mating. The method significantly improves the estrus concentration of ewes, the utilization rate of semen and the pregnancy rate, and is suitable for the reproductive management of large-scale and intensive sheep farms.

The application provides a horse serum albumin and a preparation method and application thereof, and relates to the technical field of protein engineering. The application first carries out mutation on horse serum albumin to obtain a beneficial mutation site, and further obtains horse serum albumin and an engineering bacterium which can be highly expressed in a Pichia pastoris system. Moreover, the horse serum albumin mutant prepared by the method disclosed in the application also has excellent stability. The application is suitable for industrial production and preparation of horse serum albumin related products.

The invention belongs to the technical field of optical imaging and biosensing, and particularly relates to a norepinephrine activated fluorescent probe as well as a preparation method and application thereof. Morpholine groups are added into a fluorescent chlorine-hydroxyl-cyanine support of the fluorescent probe, the hydrophilicity of fluorophores can be further enhanced due to the fact that the morpholine groups have hydrophilicity, and meanwhile the morpholine groups have alkalescence and are prone to protonation, so that the morpholine groups can better remain in acidic vesicles of cells to respond to NE, and the fluorescent probe can be used for detecting the NE in the acidic vesicles of the cells. Further, the response speed and the recognition capability of probe molecules in living cells and horse serum are greatly improved.