56 results about "Mature adipocytes" patented technology

The present invention relates to methods of mitigating, preventing or treating weight gain or obesity in patients by administering one or more autophagy inhibitors, thereby, preventing the differentiation process of pre-adipocyte cells into a mature adipocytes. Specifically, the present invention relates to the surprising discovery that autophagy is critical for the cellular remodeling required during pre-adipocyte differentiation into mature adipocyte. By targeting and inhibiting one or more mechanisms in autophagy, adipocyte maturation is also inhibited, thus, providing a novel a pathway to prevent, mitigate and/or treat weight gain, obesity and associated diseases, such as type II diabetes.

A pharmaceutical composition including, as an active ingredient, one of the following proteins (I) and (II): (I) an apoptosis inhibitor of macrophage; and (II) a protein which consists of an amino acid sequence having deletion, substitution, or addition of one or more amino acids in an amino acid sequence of the apoptosis inhibitor of macrophage and having homology to the amino acid sequence of the apoptosis inhibitor of macrophage, and has a function of inhibiting the differentiation of preadipocytes to mature adipocytes and/or a function of inducing lipolysis in the mature adipocytes.

The invention discloses an in-situ adipocyte composite inhibitor for the local weight loss and body care of human bodies and a preparation method thereof. The compound (composite inhibitor) comprises the following bulk drugs: anti-NPY monoclonal antibodies, alpha-aminoethanesulfonic acid, mannitol, enol phosphatidylcholine, sodium deoxycholate, trans-10, cis-12conjugated linoleic acid, docosahexenoic acid, berberine, emodin, sodium bicarbonate and injection water. The compound of the invention can synchronously inhibit the propagation and the differentiation of preadipocytes, accelerate and promote the decomposition and the conversion of fats in mature adipocytes, effectively reduce the quantity of in-situ and newborn mature adipocytes, regulate the expression, the synthesis and the secretion of relevant adipogenic genes and adipocyte growth factors from the gene level and the like, and control the propagation and the fat synthesis of the adipocytes at the source; and, additionally, the absorption utilization rate of the compound is improved through local administration, so the compound has the rapid and efficient weight loss effect.

The invention discloses a method for efficiently preparing a fat source biomaterial by using ultrasonic wave. The method comprises the steps of centrifugation, ultrasonic wave breaking (including contact type and non-contact type ultrasonic wave), centrifugal collection and the like. According to the method, the ultrasonic wave is utilized to break most mature fat cells in fat tissues, and the flocculation centrifugal sedimentation technology is applied to collect oil droplets generated by breaking the mature fat cells to achieve the purpose of enriching SVFs; high-speed centrifuging is utilized to remove cellular components to obtain ECM. After ultrasonic treatment, the cell activity is greatly improved, and the apoptosis rate is greatly reduced, so that the effectiveness of the transplantation is greatly improved. At the same time, an ECM biomacromolecule material can be obtained, and the low ECM content and the cytokine loss caused by the cumbersome operation are avoided. The biomaterial obtained by the method can be used for the autologous transplantation biological therapy, reduces the complications of fat transplantation and improves the survival rate of transplanted fat.

The invention discloses a preparation method of adipose gel enriched in an adipose-derived stem cell and an extracellular matrix. A mature adipose cell and the extracellular matrix in an adipose transplanting material are crushed through a physical cutting method; the filtration and the emulsification are carried out by using an adipose emulsifier, so as to remove an uncrushed component in adipose and obtain chylous adipose; by adopting a flocculation precipitation technique, grease in the chylous adipose is collected and removed by using polyethylene glycol, so as to obtain the adipose gel rich in the adipose-derived stem cell and the extracellular matrix. According to the adipose gel obtained by the method, as the mature adipose cell and the grease are removed, the volume of original adipose is concentrated for approximately 10 times; the content of the adipose-derived stem cell and the extracellular matrix is far higher than that of common adipose; a favorable material is provided for the transplantation and filling of the adipose.

The invention discloses a layered co-culture method for intramuscular fat cells and skeletal muscle cells of a mammal. The layered co-culture method comprises the steps: after washing and cutting a longissimus tissue on the back of a butchered animal into pieces, digesting the longissimus tissue by using type-I collagenase, and sieving a digested mixed solution; collecting filtrate, centrifuging, respectively collecting mature fat cell sap on the upper layer and muscle cells on the lower layer, respectively adding a proper amount of serum-free medium to dilute and wash, and centrifuging; adding the centrifugate on the upper layer and the muscle cell sap on the lower layer into a culture flask with the capacity of 25cm<2>, and culturing in a culture box with the temperature of 37 DEG C and the CO2 content of 5%. The mature fat cells can float and adhere to a ceiling surface on the upper layer because of small buoyancy, while muscle cells sink to the bottom of the culture flask to grow. The cell culture model can be used for layering and co-culturing the intramuscular fat cells and the muscle cells of the same muscle tissue of an animal in the same culture solution, so that a novel in-vitro cell research model is provided for researching the influence of mature fat cell secreta to muscle cell differentiation or the interaction between the muscle cells and the fat cells.

In one embodiment, the present invention relates to a non-enzymatic method for isolating stem cells from adipose tissue, wherein the method comprises treating adipose tissue with ultrasonic cavitation to break up the adipose tissue and lyses mature adipocytes, resulting in a stromal vascular fraction containing viable stromal/stem cells.

The invention belongs to the field of pharmaceutical preparation, and particularly provides an application of berberine in preparation of a medicine used for inhibiting autophagy of adipocyte and a medicine used for treating obesity. According to the application, C3H10T1/2 cell is cultured and induced to be differentiated into mature adipocyte, then the test for the impact of berberine to the mature C3H10T1/2 adipocyte autophagy marker protein LC3 and an autophagy substrate P62, and the test for detecting the C3H10T1/2 flat autophagy flux variation through chloroquine serving as a lysosomal inhibitor are performed, and the variation of the quantity of autophagosome can be observed through a transmission electron microscope; the results of various tests show that the berberine can effectively inhibit the autophagy of C3H10T1/2 adipocyte and provides theoretical basis for clinically reasonably applying the berberine to the treatment of obese patients; in addition, the berberine also can be applied to the preparation of the medicine for inhibiting the autophagy of adipocyte.

The invention discloses an autophagy monitoring method for fat cells. Due to the adoption of a special structure, mature fat cells are full of plenty of lipid droplets, so that organelle and nucleus are displaced towards the periphery, and a typical autophagosome is hard to be seen under an electron microscope. Proved by a plurality of experiments, the method disclosed by the invention finally and successfully observes autophagy structures in various autophagy stages in mature 3T3-L1 fat cells by continuously improving the experiment condition. Besides, mature fat has a characteristic of being not easy to be transfected, so common plasmid transfection is hard to play an expectant effect. Therefore, the method disclosed by the invention utilizes a GFP-LC3 lentivirus method to infect 3T3-L1 fat cells, and the infection efficiency is as high as 80% or greater by observation under a fluorescence microscope. At nutrition and hunger condition, by the method disclosed by the invention, GFP-LC3 point-like aggregation phenomenon is successfully observed. The establishment of the autophagy monitoring method for mature fat cells lays the foundation of research on the relationship between autophagy and fat metabolism for us.

The invention discloses a culture dish for mature adipocyte ceiling culture, and belongs to the technical field of biological medicine experimental devices. One or more inverted small culture dishes are arranged in an upright large culture dish; a U-shaped tube is arranged in the large culture dish; one end of each U-shaped tube is exposed outside the culture solution of the large culture dish; one end of each U-shaped tube is positioned inside each inverted small culture dish. No air is retained at the bottoms inside the inverted small culture dishes; meanwhile, exchange of liquids inside the inverted small culture dishes with external air can be promoted; meanwhile, the U-shaped tubes can be connected with a sampling device, and then mature adipocyte can be injected into the inverted small culture dishes for culture. The culture dish is reasonable in structural design, simple to operate, and capable of effectively culturing mature adipocyte.

The invention belongs to the technical field of regenerative medicine and discloses a method for preparing an adipose-derived ECM (extracellular matrix) through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, manure adipose cells and SVF cells are removed, the ECM is prepared, and too low ECM content and cell factor loss caused by complex operation are avoided effectively. According to the method, the ECM rich in cell factors is prepared without addition of exogenous chemical substances, the prepared ECM contains no exogenous substances, and adipose autotransplantation and effectiveness of the ECM as a biological filling material can be improved. The adipose-derived ECM material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

A method wherein differentiation of precursor fat cell lines originating in swine and mouse matured fat cells is induced so as to give cells having other functions; and cells having other functions acquired by this method. By inducing differentiation of precursor fat cell lines originating in swine and mouse matured fat cells, it is possible to acquire osteoblasts, myoblasts, chondrocytes and neurocytes. By inducing differentiation of a precursor fat cell line originating in mouse matured fat cells, furthermore, it is possible to acquire epitheliocytes.

The present invention relates to a method for culturing adipocytes, in which a homogeneous population of mature adipocytes isolated from adipose tissue is cultured on a three-dimensional culture substrate.

The invention relates to an adipose tissue forming method based on in-vitro induction of a vascularized pedicle. The adipose tissue forming method comprises the following steps: taking an adipofascialtissue of the vascularized pedicle as a living support, inducing seed cells with mature adipocytes as main bodies in situ, performing in-vitro perfusion and transdifferentiation by using a muscle forming culture solution to obtain skeletal muscle cells, and constructing an axial vascularized tissue engineering skeletal muscle tissue in vitro. The adipose tissue forming method has the beneficial effects: under the premise of alleviating body damage to the maximum degree, the axial vascularized tissue engineering skeletal muscle tissue is cultured for treating muscle defects.

The invention discloses a fish high-fat storage hepatocyte separation and culture method. The method comprises the following steps: a, selecting 45-55d young gobius giurinus, obtaining liver tissues of the young gobius giurinus under a sterile condition, and dispersing the liver tissues into blocks to obtain tissue blocks; b, putting the cell sieve with the pore diameter of 20 microns into a trypsin stop solution, and arranging the cell sieve with the pore diameter of 50 microns above the cell sieve with the pore diameter of 20 microns; c, putting the tissue blocks into a cell sieve with the pore diameter of 50 microns, continuously adding trypsin liquid into the cell sieve with the pore diameter of 50 microns to digest the tissue blocks, continuously digesting by flowing, collecting cellson the cell sieve with the pore diameter of 20 microns, and cleaning with 10% FBS to obtain high-fat storage liver cells; and d, inoculating the high-fat storage hepatocytes into a culture medium forculturing. The high-fat storage hepatocytes can be stabilized for one week after adherence, adipose cells can survive for about one month, and the high-fat storage hepatocytes can be used for cell level experiments in the period so that the problem that mature fish adipose cells are difficult to separate and culture is solved.

The invention belongs to the technical field of regenerative medicine and discloses a preparation method for efficiently enriching a SVF (stromal vascular fraction) cell material through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, and meanwhile, oil drops produced by crushed mature adipose cells are collected with a floccus centrifugal precipitation technology, so that the effect of enriching SVFs is realized. After ultrasonic treatment, the cell viability is substantially improved and the cell apoptosis rate is substantially decreased, so that the transplanting effectiveness is greatly enhanced. The cell material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

The invention discloses an application of streptococcus thermophilus MN002 in lipid metabolism regulation and a dietary supplement, and discloses an application of streptococcus thermophilus MN002 in preparation of a product with a lipid metabolism regulation function. The product is used for inhibiting expression of fat related transcription factors, inhibiting preadipocytes from differentiating into mature adipocytes, and further reducing fat cell accumulation and reducing secretion of fat-related cell factors. The invention also provides the dietary supplement which contains the inactivated streptococcus thermophilus MN002 and has a lipid metabolism regulation function. The effect of regulating lipid metabolism is achieved.

A process for screening the active weight losing-hypolipemic component from Chinese-medicinal materials features that one or more of fatty acid synthetase, acyl-CoA synthetase and the differentiationof mouse and human pre-fat cells to mature fat cells are combined as target of said screening. Its advantages are clear target, and large-scale screening.

A method for producing a mesenchymal cell line derived from a vertebrate adipose tissue, and a mesenchymal cell line derived from a vertebrate adipose tissue produced by the method. Advantageously, a method for producing a mesenchymal cell line derived from a vertebrate adipose tissue is achieved more simply, in a shorter period of time, and more efficiently. Also, a mesenchymal cell line is derived from a vertebrate adipose tissue produced by the production method. The method for producing a mesenchymal cell line derived from a vertebrate adipose tissue comprises: (A) inducing differentiation of one or more cells selected from a stromal vascular fraction comprising a mesenchymal stem cell, an adipose progenitor cell, and a stromal cell of a vertebrate adipose tissue into a mature adipocyte; and (B) inducing dedifferentiation of the mature adipocyte obtained in step (A) to obtain a mesenchymal cell line derived from the vertebrate adipose tissue.

The invention relates to the technical field of cell culture and discloses isolated culture and an induction method of nibea albiflora precursor adipose cells. The isolated culture comprises the following steps: 1) firstly, digesting nibea albiflora adipose tissue blocks by utilizing a pancreatin solution; then treating the nibea albiflora adipose tissue blocks by utilizing a type II collagenase digestion solution; and 2) inoculating dissociated nibea albiflora precursor adipose cells into a cell culture bottle, and culturing by adopting a precursor adipose cell culture medium to obtain a cellsingle layer. The induction method comprises the following steps: after carrying out digestion treatment on the obtained nibea albiflora precursor adipose cells through the pancreatin solution, inoculating into a 6-pore plate and culturing; and then transferring into a precursor cell induction culture medium and continually culturing. According to the isolated culture and the induction method, the nibea albiflora precursor adipose cells can be successfully obtained and are further induced to obtain mature adipose cells; the isolated culture and the induction method have the advantages of simple and feasible method, good repeatability, low cost and rapid cell growth; and an effective in vitro cell model can be provided for molecular cell biology researches of nutrition metabolism of nibeaalbiflora.