59 results about "Autologous transplantation" patented technology

An implant composed substantially of cortical bone is provided for use in cervical Smith-Robinson vertebral fusion procedures. The implant is derived from allograft or autograft cortical bone sources, is machined to form a symmetrically or asymmetrically shaped (e.g. a substantially "D"-shaped) implant having a canal running therethrough according to methods of this invention, and inserted into the space between adjacent cervical vertebrae to provide support and induce fusion of the adjacent vertebrae. Osteogenic, osteoinductive or osteoconductive materials may be packed into the canal of the implant to expedite vertebral fusion and to allow autologous bony ingrowth.

Composite orthopedic devices that facilitate spine stabilization, such as: bone screws, rods, plates, interbodies, and corpectomy cages are disclosed. They are designed to provide both strength and load carrying capabilities, while increasing bio-integration of the devices with the surrounding bone tissue. They are constructed of composite layers of allograft and / or autograft bone and a structural material, such as titanium alloy or carbon / graphite fiber composite. Cannulations within the device are loaded with a mixture of stem cells, particles of allograft and / or autograft bone, and bone growth factors, such as BMP-2. The cannulations are connected to the surface of the device via multiple fenestrations that provide pathways to supply the bone / stem cell mixture to the surface, allowing living bone tissue to grow and insure bio-integration. The devices can also have radiofrequency (RF) stimulation implantation within the structure of the implanted device, capable of responding to external RF stimulation of enhanced bone growth.

The invention provides a test method for predicting the initial onset or a recurrence of acute myeloid leukemia (AML) comprising (1) measuring the expression level of human leukemic stem cell (LSC) marker genes in a biological sample collected from a subject for a transcription product or translation product of the gene as an analyte and (2) comparing the expression level with a reference value; an LSC-targeting therapeutic agent for AML capable of suppressing the expression of a gene selected from among LSC marker genes or a substance capable of suppressing the activity of a translation product of the gene; a method for producing a sample containing hematopoietic cells for autologous transplantation or allogeneic transplantation for AML patients comprising obtaining an LSC-purged sample with at least 1 kind of LSC marker as an index; and a method of preventing or treating AML.

The invention provides methods and compositions for differentiating stromal cells from adipose tissue into cells having osteoblastic properties, and methods for improving a subject's bone structure. The methods comprise culturing stromal cells from adipose tissue in β-glycerophosphate and ascorbic acid and / or ascorbate-2-phosphate for a time sufficient to allow differentiation of said cells into osteoblasts. Such methods and compositions are useful in the production of osteoblasts for autologous transplantation into bone at a surgical site or injury. The compositions comprise adipose stromal cells, a medium capable of supporting the growth of fibroblasts and amounts of β-(glycerophosphate and ascorbic acid and / or ascorbic-2 phosphate sufficient to induce the differentiation of said stromal cells into osteoblasts.The invention further provides methods of identifying compounds that affect osteoblast differentiation. Such compounds are useful in the study of bone development and in the treatment of bone disorders, including bone fractures and osteoporosis.

Current sources of neural stem and progenitor cells for neural transplantation are essentially inaccessible in living animals. This invention relates to neural precursor cells (stem cells, progenitor cells or a combination of both types of cells) isolated from the olfactory epithelium of mammals that can be passaged and expanded, and that will differentiate into cell types of the central nervous system (CNS), including astrocytes, oligodendrocytes, and tyrosine-hydroxylase-positive neurons. These precursor cells provide an accessible source for autologous transplantation in CNS, PNS, spinal cord and other damaged tissues.

An implantable article for cartilage repair by implantation in an animal. The implantable article includes a support matrix, and chondrocyte cells and a bio-compatible adhesive adhered to an edge of the support matrix, wherein the support matrix is absorbable by the animal. The support matrix is a biocompatible material such as collagen, and the adhesive is autologous fibrin.

Milling procedure performed on the patient's bone or other tissue in order to form in said tissue a cavity (5) of a shape and size that allows it to house an implant (4) (or for other purposes where the tissue must regenerate) wherein milling is performed at low speeds and without irrigation without the tissue heating up and necrosis occurring in the tissue. The special design of the mill tools (8, 9) enables the tissue particles extracted during the milling process to be collected without using a suction machine. Said particles are in optimal biological condition for use in autografting due to the fact that neither over heating nor irrigation occur during the milling process.

An implantable article for cartilage repair by implantation in an animal. The implantable article includes a support matrix, and chondrocyte cells and a bio-compatible adhesive adhered to an edge of the support matrix, wherein the support matrix is absorbable by the animal. The support matrix is a biocompatible material such as collagen, and the adhesive is autologous fibrin.

The invention is directed to the field of human stem cells and includes methods and compositions for isolating, propagating, and differentiating human stem cells. The invention provides therapeutic uses of the methods and compositions, including autologous transplantation of treated cells into humans for treatment of Parkinson's and other neuronal disorders.

A method is described for isolating ensheathing cells, in particular those from olfactory lamina propria and use of the isolated ensheathing cells and lamina propria respectively in transplantation. Isolated lamina propria and ensheathing cells from the olfactory mucosa are well suited for autologous transplantation, where the donor and recipient are the same, as surgical biopsy of the olfactory mucosa is less damaging than isolating tissue from other location of a person's body, for example the olfactory bulb. Transplantation is particularly directed to neural regions (for example the brain, spinal cord and / or peripheral nerves) of a human to assist recovery of acute and chronic nerve damage following surgery or trauma.

A system and method for the percutaneous, autologous transplantation of mesenchymal stem cells and progenitor helper cells (PHC) from bone marrow to degenerated intervertebral discs or joints. This method is designed to be used by operating room staff in a clinical setting to isolate a mesenchymal stem cell population and PHC during the same surgical procedure as transplantation. The method can be used as a two step procedure where cells are harvested, then isolated, then reimplanted at a later time. In addition, experimental techniques are described to determine which bone marrow cells should be removed via negative selection to generate a PHC population most likely to regenerate certain tissue types in-vitro as well as which combination of fibrinogen and hyaluronic acid and which degree of gel maceration provides the best matrix for in-vitro and in-vivo regeneration of joints and intervertebral discs.

It is intended to provide anticancer therapy using autologous cells or the like, which induces the regression of cancer or has favorable drug delivery effects and brings about reduction or withdrawal of a hypoxic region(s) in tumor. Transplantation of endothelial progenitor cells, via intravenously or other methods leads to tumor growth inhibition, an increase of the vascular density with an enlargement of the vascular diameter, and reduction of a hypoxic region(s) in the tumor. Allogeneic transplantation of endothelial progenitor cells may be achieved to secure the cells for the therapy, however, autologous transplantation of endothelial progenitor cells from cancer patients would be desirable in order to evade rejection. When the autologous cells are used, mononuclear cells are separated from the peripheral blood or bone marrow of the patient and cultured using an endothelial differentiation medium containing cytokines such as VEGF to obtain adherent cells, which can then be collected and advantageously used as endothelial progenitor cells.

The present invention describes various support matrices to which cells can adhere and proliferate. Such support matrices are useful for implantation in a wound site to promote healing and regeneration of damaged tissue. The present invention further describes an article including a membrane having at least one layer having a porous surface and also including submucosal intestine tissue, and cells adhered to the layer. The present invention further describes that the cells adhered to the layer include chondrocyte cells.

The invention provides methods and compositions for differentiating stromal cells from adipose tissue into cells having osteoblastic properties, and methods for improving a subject's bone structure. The methods comprise culturing stromal cells from adipose tissue in β-glycerophosphate and ascorbic acid and / or ascorbate-2-phosphate for a time sufficient to allow differentiation of said cells into osteoblasts. Such methods and compositions are useful in the production of osteoblasts for autologous transplantation into bone at a surgical site or injury. The compositions comprise adipose stromal cells, a medium capable of supporting the growth of fibroblasts and amounts of β-glycerophosphate and ascorbic acid and / or ascorbic-2 phosphate sufficient to induce the differentiation of said stromal cells into osteoblasts. The invention further provides methods of identifying compounds that affect osteoblast differentiation. Such compounds are useful in the study of bone development and in the treatment of bone disorders, including bone fractures and osteoporosis.

The invention discloses a method for efficiently preparing a fat source biomaterial by using ultrasonic wave. The method comprises the steps of centrifugation, ultrasonic wave breaking (including contact type and non-contact type ultrasonic wave), centrifugal collection and the like. According to the method, the ultrasonic wave is utilized to break most mature fat cells in fat tissues, and the flocculation centrifugal sedimentation technology is applied to collect oil droplets generated by breaking the mature fat cells to achieve the purpose of enriching SVFs; high-speed centrifuging is utilized to remove cellular components to obtain ECM. After ultrasonic treatment, the cell activity is greatly improved, and the apoptosis rate is greatly reduced, so that the effectiveness of the transplantation is greatly improved. At the same time, an ECM biomacromolecule material can be obtained, and the low ECM content and the cytokine loss caused by the cumbersome operation are avoided. The biomaterial obtained by the method can be used for the autologous transplantation biological therapy, reduces the complications of fat transplantation and improves the survival rate of transplanted fat.

The invention discloses an in vitro construction method for biological engineering salivary glands organs and acinus based on human minor salivary gland adult stem cells belonging to the technical field of tissue and organ reconstruction. The in vitro construction method specifically is a method for obtaining organoids with salivary gland structures and functions by mixed cultivation of mesenchymal stem cells and epithelial stem / progenitor cells of the human minor salivary glands, or a method for obtaining acinar-like tissues with salivary gland structures and functions by individual cultivation of epithelial stem / progenitor cells of the human minor salivary glands. The method has the advantages of being wide in sampling source, easy to cut, and small in trauma; a donor site in a mouth is private; materials are drawn from adult tissues; and autoplastic transplantation can be carried out through amplification in vitro. The method has a wide application prospect in growth and development research of in vitro or in vivo salivary glands, research on a salivary gland disease model, research on physiological functions and pathological changes of the saliva and treatment of salivary gland diseases.

A method and a device for autografting of fat microparticles containing stem cells of various types, injected into scars, or stretch marks, and / or other cutaneous injuries, at epidermis-dermis level is presented. Fat is taken from the patient in the periumbilical area, where large quantities of mesenchymal stem cells exist. After reduction in size by means of a thin grid emulsifier filter the fat microparticles are loaded into a tank of a multi-needle gun, such as used for tattoos. The multi-needle tattoo gun injects the fractionated fat microparticles into the epidermis-dermis of scars, and / or stretch marks and / or other skin defects or even internal injuries. The injected fat particles trigger tissue regeneration, deleting scars, stretch marks, wrinkles and / or other defects or skin damages in 3-4 weeks. This method for regeneration of tissues can be extended to all surgeries, including vital organs of the human body; always by means of tattooing the target organ with autologous fat microparticles containing stem cells, wherein access is through open, endoscopic, and / or laparoscopic surgery.

The invention relates to the field of the biotechnology, in particular to an adipogenesis induction culture medium and an adipogenic differentiation method. The adipogenesis induction culture medium comprises 3-isobutyl-1-methylxanthine, insulin, indomethacin, dexamethasone, rosiglitazone, PRP (Platelet Rich Plasma) and a basic culture medium. The invention provides an adipogenic differentiation method which enables various inducing factors to be subjected to combined application to act on mesenchymal stem cells including GMSCs and like, has a synergistic effect and can effectively induce the adipogenic differentiation of the mesenchymal stem cells, and the adipogenic differentiation effect of the adipogenic differentiation method is obviously superior to that of an existing conventional induction method. The GMSCs autologous materials are abundant, are easy in expansion in vitro and can be used for autotransplantation so as to avoid immunological rejection reaction.

The invention discloses an extracting and in-vitro multiplication culture technical method of urine-derived mesenchymal stem cells (USCs). In-vitro multiplication is mainly conducted in the mode thatthe mesenchymal stem cells (MSCs) are separated from sterile urine, and a culture medium easy to prepare is adopted; the extracted USCs subjected to multiplication culture can be used for therapy of clinical immunology related diseases and damage repair of various tissue and organs; the extracting and in-vitro multiplication culture technical method has the advantages that the separating and extracting processes of the USCs are completely free of wounds, special instruments are not needed, the cost is low, and the extracting process is easy to accept by patients; the USCs have other advantagesof being capable of achieving autotransplantation, free of immune rejection response and ethical arguments, and capable of avoiding the pathophoresis risk; and the USCs show the good multiplication ability, and the number of the cells needed during cell therapy can be met.

The present invention concerns a composition useful as bone substitute comprising one or more calcium-phosphate compounds in association with an analgesic. It also refers to a preparation process of said composition, a preparation process of a drug-combined device comprising said composition, the drug combined device thus obtained, a kit comprising said composition and the use of said composition for the preparation of a drug-combined device useful for filling a bony defect caused in the iliac crest by collection of auto-graft bone, as a scaffold for tissue engineering and to produce a dental or bony implant.

This invention provides a process of obtaining a functional dermal substitute from decellularized amniotic membrane from placenta in combination with mammalian keratinocytes and its use as a tissue regeneration agent of skin, which the process for the generation of an artificial skin graft in the laboratory using specialized techniques in biotechnology such as the mammalian cells culture, preferably human, in which keratinocytes are cultured in aseptic conditions that form part of the epidermal layer of the skin, which are combined with a mesh rich in collagen which is obtained from cells that compose the structure of the skin in combination with the amniotic membrane of the placenta to generate two layers that can be used in burn patients or with skin lesions. Additionally, this technology considerably reduces the cost of treatment compared to the cost of current methods such as skin graft treatments (autografts or allograft) and the result of this process is a prompt cellular reorganization, remodeling, and re-epithelization of the wound resulting in the formation of new skin tissue and avoiding the formation of fibrotic tissue that results from contraction muscle.

The invention belongs to the technical field of regenerative medicine and discloses a method for preparing an adipose-derived ECM (extracellular matrix) through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, manure adipose cells and SVF cells are removed, the ECM is prepared, and too low ECM content and cell factor loss caused by complex operation are avoided effectively. According to the method, the ECM rich in cell factors is prepared without addition of exogenous chemical substances, the prepared ECM contains no exogenous substances, and adipose autotransplantation and effectiveness of the ECM as a biological filling material can be improved. The adipose-derived ECM material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

This invention relates to multipotent stem cells, purified from the peripheral tissue of mammals, and capable of differentiating into neural and non-neural cell types. These stem cells provide an accessible source for autologous transplantation into CNS, PNS, and other damaged tissues.

A bimodal excitation cell system based on ultrasonics and electromagnetics belongs to the field of medical equipment. The system consists of a power module, a signal generating module, a driving amplification module, an ultrasonic excitation module, a magnetic excitation module and a detection module. The signal generating module transmits a pulse signal to the driving amplification module; the pulse signal is directly transmitted to the ultrasonic excitation module and the detection signal module after processed by the driving amplification module; the detection signal module is used for detecting the quality of the signal output at any time; the power module supplies power to the ultrasonic excitation module and the magnetic excitation module; the magnetic excitation module generates a magnetic field and acts on the cell excitation operation with the ultrasonic excitation module together. The system overcomes the limitation of single modal excitation cells, and can further increase the proliferation and the activity of the cells, thereby providing sufficient seed cells with good activity for autologous transplantation and providing more hope for treatment of traumatic paraplegia.

In order to solve the problems of slow effect, unsatisfactory effect and the like in a conventional adipose tissue derived stem cells (ADSCs) transplant beauty technology, the invention provides a cosmetic preparation containing ADSCs as well as a preparation method and application of the cosmetic preparation. The cosmetic preparation provided by the invention is one or a combination of an ADSCs suspension and an ADSCs lysate; the ADSCs suspension is prepared by directly filtering with a 100-mesh screen after adipose tissues are obtained by water flow-assisted liposuction; the ADSCs lysate is obtained by freezing, crushing and cracking the ADSCs suspension. The cosmetic preparation provided by the invention has good treatment effect through one-time cosmetic operation, long maintenance time, higher survival rate than separate autologous ADSCs transplantation, short treatment time and better treatment effect.

A system for performing a minimally invasive sacroiliac joint fusion. The system may be in the form of a disposable kit, with the components streamlined so that the procedure can be performed in a few minutes. The screw components are self-drilling and self-tapping. The system may deploy blades through the walls of the primary screw which cut away material as the primary screw is set, for denuding the sacroiliac joint. The primary screw is designed bore through and internalize bone tissue in an autografting process. The implant system may include components for packing bone grafting material into the screw to supplement autograft bone tissue internalized in the primary screw during placement. At least one side screw is passed through a head of the primary screw to anchor the head and prevent it from backing out after implantation.

The invention belongs to the technical field of regenerative medicine and discloses a preparation method for efficiently enriching a SVF (stromal vascular fraction) cell material through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, and meanwhile, oil drops produced by crushed mature adipose cells are collected with a floccus centrifugal precipitation technology, so that the effect of enriching SVFs is realized. After ultrasonic treatment, the cell viability is substantially improved and the cell apoptosis rate is substantially decreased, so that the transplanting effectiveness is greatly enhanced. The cell material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

The present invention concerns a new method for ex-vivo purging of cells in autologous transplantation, wherein the sample of taken cells is treated with a sufficient amount of a multimeric form of the soluble portion of FasL to kill malignant cells without substantially affecting viability of cells to be transplanted. Autologous stem cell transplantation (ASCT) following high-dose chemotherapy with or without radiotherapy has become the standard therapy for the majority of patients with large-cell lymphomas, multiple myeloma, and refractory / recidivating Hodgkin's disease. Such therapy is nowadays also contemplated for selected patients with low-grade lymphomas (chronic lymphocytic leukemia, follicular lymphoma, mantle cell lymphoma) and for patients with acute myeloid leukemia (AML). Current treatments for cell purging include chemotherapy and antibody cocktails. These treatments are often toxic on stem cells and not efficient in eliminating cancer cells. Thus, there is an unmet medical need for cell purging in ASCT which this project will address.