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Cosmetic preparation containing adipose tissue derived stem cells as well as preparation method and application of cosmetic preparation

A technology of fat stem cells and preparations, applied in the field of medicine, can solve problems such as unsatisfactory effects and slow onset of effects, and achieve the effects of smooth and supple skin, shrinking pores, and long-lasting effects

Inactive Publication Date: 2017-09-01
JIANSHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems of slow onset and unsatisfactory effects in the existing cosmetic technology of adipose stem cell (ADSCs) transplantation, the present invention proposes a cosmetic preparation containing fat stem cells and its preparation method and application

Method used

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  • Cosmetic preparation containing adipose tissue derived stem cells as well as preparation method and application of cosmetic preparation
  • Cosmetic preparation containing adipose tissue derived stem cells as well as preparation method and application of cosmetic preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A cosmetic preparation containing adipose stem cells, the preparation is a suspension of adipose stem cells, which is a suspension of adipose stem cells obtained from the patient itself through water-assisted liposuction and directly filtered through a 100-mesh sieve. liquid.

Embodiment 2

[0032] A cosmetic preparation for containing fat stem cells, the preparation is a fat stem cell lysate, and the fat stem cell lysate is obtained by the following preparation method:

[0033] S1. Obtain the patient's own adipose tissue through water-assisted liposuction;

[0034] S2, filtering the adipose tissue obtained in step S1 through a 100-mesh sieve to prepare adipose stem cell suspension;

[0035] S3. Put the adipose stem cell suspension obtained in step S2 into a cryopreservation tube, immediately put it into a liquid ammonia tank at -196°C, freeze it for 10 minutes, then take it out and thaw it, and mix it vigorously on an electric mixer for 5 minutes. Adipose stem cells are fully broken and lysed;

[0036] S4. Put the adipose stem cell solution obtained in step S3 into a liquid ammonia tank at -196°C, freeze for 10 minutes, take it out and melt it, and mix vigorously on an electric mixer for 5 minutes to fully break and lyse the adipose stem cells;

[0037] S5. Cen...

Embodiment 3

[0039] A cosmetic preparation for containing adipose stem cells, the preparation is adipose stem cells and adipose stem cell lysate, and the preparation is obtained by the following preparation method:

[0040] S1. Obtain the patient's own adipose tissue through water-assisted liposuction;

[0041] S2, filtering the adipose tissue obtained in step S1 through a 100-mesh sieve to prepare adipose stem cell suspension;

[0042] S3. Divide the adipose stem cell suspension obtained in step S2 into two parts, one for use, and the other for preparing adipose stem cell lysate, put the latter into a cryopreservation tube, and immediately put it into a liquid ammonia tank at -196°C , frozen for 10 minutes, then removed and thawed, and vigorously mixed on an electric mixer for 5 minutes to break and lyse the adipose stem cells;

[0043] S4. Put the crushed and lysed adipose stem cell liquid obtained in step S3 into a -196°C liquid ammonia tank, freeze for 10 minutes, then take it out and...

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Abstract

In order to solve the problems of slow effect, unsatisfactory effect and the like in a conventional adipose tissue derived stem cells (ADSCs) transplant beauty technology, the invention provides a cosmetic preparation containing ADSCs as well as a preparation method and application of the cosmetic preparation. The cosmetic preparation provided by the invention is one or a combination of an ADSCs suspension and an ADSCs lysate; the ADSCs suspension is prepared by directly filtering with a 100-mesh screen after adipose tissues are obtained by water flow-assisted liposuction; the ADSCs lysate is obtained by freezing, crushing and cracking the ADSCs suspension. The cosmetic preparation provided by the invention has good treatment effect through one-time cosmetic operation, long maintenance time, higher survival rate than separate autologous ADSCs transplantation, short treatment time and better treatment effect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a cosmetic preparation containing fat stem cells, a preparation method and application thereof. Background technique [0002] With the gradual improvement of people's material living standards, it is no longer a problem for people to have enough food and clothing. The affluence of life makes people start to pursue a higher level of life. Everyone has a desire for beauty, and everyone hopes to be young and beautiful forever. Many people turn their attention to various methods that can improve their appearance. As the largest tissue and organ of the human body, the skin covers the appearance of the human body and is the main tissue and organ for the study of aesthetics and cosmetology. All of a sudden, various cosmetics and skin care products have sprung up like mushrooms after rain. However, these cosmetics on the market only achieve the purpose of beauty by covering up defects...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K8/99A61Q19/08A61P17/18A61P17/00A61L27/38
Inventor 刘国卿张彬
Owner JIANSHENG BIOTECH CO LTD
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