The invention belongs to the technical field of preparation of probiotics, and relates to a preparation method of a composite
Pickering emulsion for improving the
survival rate of probiotics. The preparation method comprises the following steps: adding span-80 into
evening primrose oil, uniformly stirring the span-80 and the
evening primrose oil to prepare an
oil phase, adding a washed probioticssolution in the
oil phase, and carrying out homogenizing to obtain a product A; and mixing a
cyclodextrin aqueous solution with the product A to obtain the composite
emulsion. According to the preparation method, a double-layer emulsification
system is adopted, firstly, a high
internal phase processing technology can ensure high content of probiotics in an internal water phase, secondly, the
antioxidant activity of the
evening primrose
oil phase can prevent the probiotics from being oxidized and damaged after the probiotics are stored and orally taken, and in addition, a nano-scale
Pickering emulsion wall layer formed by beta-
cyclodextrin on the outer layer has a very stable protection effect, can protect the probiotics from being damaged when passing through the
stomach and the
small intestine, improves the
survival rate of the probiotics, and enables the probiotics to smoothly reach the
large intestine to play a role. The preparation method can be used for developing novel probioticsfoods, medicines and health products.