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Horse placenta water-soluble protein extract, preparation method and application thereof

A water-soluble protein and extract technology, which is applied in the field of horse placenta water-soluble protein extract and its preparation, can solve the problems of lack of systematic and in-depth research on horse placenta processing, and achieves enhancement of activity, improvement of enzyme activity, and reduction of cell apoptosis. The effect of mortality

Active Publication Date: 2013-11-27
XINJIANG MEDICAL UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on horse placenta at home and abroad is still in its infancy. There are almost no reports on the extraction process and biological activity of horse placenta protein, and there is still a lack of systematic and in-depth research on the processing of horse placenta.

Method used

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  • Horse placenta water-soluble protein extract, preparation method and application thereof
  • Horse placenta water-soluble protein extract, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, the horse placenta water-soluble protein extract is obtained according to the following steps: the first step, take an appropriate amount of fresh horse placenta and clean it with clear water, remove fascia and blood vessels, and cut it into pieces to obtain small pieces of horse placenta; the second step According to adding 1 milliliter to 4 milliliters of phosphate buffer saline in every gram of small piece of horse placenta, adding pH to the small piece of horse placenta is 7.2, and the molar concentration is 0.01 mole of phosphate buffer per liter. Place the small pieces of horse placenta together with phosphate buffer in a homogenizer for tissue homogenization 5 to 8 times, each tissue homogenization time is 1 min to 2 min, and the interval time of each tissue homogenization is 2 min to 4 min; In the third step, the homogenate after tissue homogenization is placed at a temperature of 30°C for 1h to 4h; in the fourth step, the homogenate after standing ...

Embodiment 2

[0015] Embodiment 2, the horse placenta water-soluble protein extract is obtained according to the following steps: the first step, take an appropriate amount of fresh horse placenta and clean it with clear water, remove fascia and blood vessels, and cut it into pieces to obtain small pieces of horse placenta; the second step According to adding 1 milliliter or 4 milliliters of phosphate buffer saline in every gram of small piece of horse placenta, adding pH to the small piece of horse placenta is 7.2, and the molar concentration is 0.01 mole of phosphate buffer per liter. Place the small pieces of horse placenta together with phosphate buffer in a homogenizer for tissue homogenization 5 or 8 times, each tissue homogenization time is 1 min or 2 min, and the interval time of each tissue homogenization is 2 min or 4 min; In the third step, the homogenate after tissue homogenization was allowed to stand at 30°C for 1 hour or 4 hours; in the fourth step, the standstill homogenate w...

Embodiment 3

[0016] Example 3, as an optimization of the above example, in the first step of Example 3, take an appropriate amount of fresh horse placenta and wash the blood stains with clear water, then rinse with distilled water for 3 to 5 times, remove the fascia and blood vessels, and cut them into pieces to obtain Small pieces of horse placenta.

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Abstract

The invention relates to the technical field of a horse placenta extract and a pharmaceutical application thereof, specifically to a horse placenta water-soluble protein extract with bioactivity, a preparation method and an application thereof. According to the present invention, during an extraction process, a phosphate buffer solution with a pH value close to a physiological pH value and an osmotic pressure close to a physiological osmotic pressure is adopted as an extraction solvent so as to maintain activity of active substances in a horse placenta to the maximal degree; with the horse placenta extract, activity of normal lymphocytes can be significantly enhanced, proliferation activity of T-lymphocytes and B-lymphocytes induced by ConA and LPS can be significantly inhibited, and killing activity of NK cells on PC-3 cancer cells can be significantly enhanced, such that adjustment effects are provided for immune functions to a certain degree; and with the horse placenta extract, oxidative damage of skin fibroblasts due to H2O2 can be significantly inhibited, cell viability can be improved, MDA content in cells can be reduced, enzyme activities of SOD and GPx can be improved, apoptosis rate can be reduced, and DNA damage can be inhibited, such that a certain protection effect is provided for oxidative damage.

Description

technical field [0001] The invention relates to the technical field of application of horse placenta extract and its medicine or / and health care product or / and cosmetics, and relates to a biologically active horse placenta water-soluble protein extract and its preparation method and application. Background technique [0002] As a temporary organ to maintain the growth and development of the fetus in the mother's body, the placenta has been valued by people since ancient times because of its special barrier function and endocrine function. , blood" role. In recent years, studies have found that the placenta contains a variety of proteins, polypeptides, polysaccharides, mineral elements, and vitamins, which have health effects such as regulating immune function, anti-oxidation, anti-aging, preventing tumors, promoting wound healing, and enhancing memory. In the study of animal placenta, the study of sheep placenta is the earliest, the deepest and the most widely used. In view...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14A61K35/50A61K38/00A61K8/98A61K8/64A61P37/04A61P17/18A61Q19/08
Inventor 高晓黎王晓然罗俊肖建民
Owner XINJIANG MEDICAL UNIV
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