11results about How to "Antiproliferative activity" patented technology

The invention provides a preparation method of dendrobium huoshanense polysaccharide, which comprises the following steps: firstly, pretreating dendrobium huoshanense through steam explosion, then extracting water-soluble polysaccharide by using a hot water extraction method, then separating out the polysaccharide by using a low-temperature alcohol precipitation method, removing protein and pigment by using a Sevag method and a macroporous resin adsorption method, and then separating out the dendrobium huoshanense polysaccharide. Dialyzing the polysaccharide solution through a regenerated cellulose dialysis bag to remove substances with the molecular weight less than 2000Da, finally freeze-drying to obtain a dendrobium polysaccharide crude product, and further separating and purifying to obtain the refined dendrobium polysaccharide. In-vitro experiments show that the refined dendrobium polysaccharide has remarkable antioxidant activity and antitumor activity, and has a better application prospect in the fields of skin care and medicines.

The invention discloses enterococcus faecalis YL-EF32 and application thereof, and belongs to the technical field of microorganisms. The collection number of the enterococcus faecalis YL-EF32 provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M 20242241. The strain can effectively inhibit the proliferation of chicken-derived clostridium perfringens, has better tolerance to cholate, artificial gastric juice and phenol, and can metabolize dietary fibers such as pectin, glucan, cellobiose and the like. Enterococcus faecalis YL-EF32 is added into daily ration of broiler chickens, necrotic enteritis induced by coccidiosis and clostridium perfringens can be remarkably improved by means of enhancing intestinal barriers, balancing intestinal flora stability, relieving inflammatory response and the like, and the enterococcus faecalis YL-EF32 can be used for resisting clostridium perfringens infection in livestock and poultry breeding.

The invention discloses a fluoromethyl substituted indeno [1, 2-b] pyridine compound with anticancer activity and a synthesis method thereof, and belongs to the technical field of organic synthesis and drug discovery. A 5-arylimidazole compound 1, a fluoromethyl acetylenic ketone compound 2, a rhodium catalyst, an additive and a solvent are mixed, a temperature rise reaction is performed to prepare the fluoromethyl substituted indeno [1, 2-b] pyridine compound, and the structural general formula of the compound is # imgabs0 #. The preparation method is completed by adopting a one-pot cascade reaction, and the process is simple and efficient; the obtained fluoromethyl substituted indeno [1, 2-b] pyridine compound has the activity of obviously inhibiting proliferation of three kinds of cancer cells such as Hela, A549 and HCT-116, so that the compound disclosed by the invention has the anti-cancer activity on cervical cancer, lung cancer and colon cancer, has potential medicinal value, and provides a new structural unit for drug screening.

The invention provides a separation and extraction method of tea fuzz, tea polysaccharide and application thereof. The separation and extraction method of the tea fuzz comprises the following steps: S1, quick-freezing fresh tea leaves through liquid nitrogen to obtain a pre-frozen tea sample; s2, crushing and sieving the pre-frozen tea sample, and collecting tealeaf fuzz; and S3, soaking the tea fuzz with saline water for 20-30 minutes, extracting with hot water, filtering, carrying out alcohol precipitation, centrifuging, separating out precipitate, carrying out deproteinization treatment on the precipitate, dialyzing and freeze-drying to obtain the tea polysaccharide in the tea fuzz. According to the method, the extraction rate of the tea polysaccharide is high, and the tea polysaccharide in the obtained tea fuzz has obvious anti-oxidation and anti-tumor activity at the same time, especially has relatively strong cancer cell proliferation inhibition activity on a human hepatoma cell line (HePG2), a human cervical cancer cell (HeLa) and a human promyelocytic leukemia cell (HL60), and has a relatively good application prospect in the fields of foods, skin care and medicines.

The invention discloses a polypentylene-based adamantane-type benzophenone compound and a preparation method and application thereof, and belongs to the field of medical technology. The polypentylene-based adamantane-type benzophenone compound of the invention is extracted with golden silk plum stems by acetone, concentrated to obtain a crude extract, suspended in water, extracted with petroleum ether and dichloromethane in sequence, the dichloromethane extract is loaded onto a silica gel chromatographic column, and after two silica gel chromatographic column purifications, the obtained eluent is recrystallized from petroleum ether to obtain. Experimental studies have shown that the compound has a very strong growth inhibitory effect on human acute promyelocytic leukemia cells HL-60, human prostate cancer cells PC-3, and human colon adenocarcinoma cells Caco-2, and has the prospect of preparing clinical tumor prevention and treatment drugs.

The application belongs to the technical field of biological medicine, and particularly relates to a dipicolinate ester derivative and application thereof. The dipicolinate ester derivative has broad-spectrum antitumor activity, can significantly inhibit the proliferation activity of lung cancer cells, pancreatic cancer cells and prostate cancer cells, and has more excellent inhibitory activity on lung cancer cells, and can significantly inhibit the growth of mouse subcutaneous small cell lung cancer transplanted tumors. In addition, the dipicolinate ester derivative has no toxic effect on normal cells of the body, can be applied to preparation of anticancer drugs, and has broad application prospects in the aspect of anticancer.

The present invention provides the use of a combined pharmaceutical composition in the preparation of a drug for preventing and / or treating liver cancer. The present invention first discovered that Cpd-63 can inhibit the proliferation, migration and invasion activity of liver cancer cells, and further combined sorafenib and Cpd-63 to produce a synergistic inhibitory effect on the proliferation of liver cancer cells. Therefore, it can be used as a combined pharmaceutical composition in the preparation of a drug for preventing and / or treating liver cancer, and has clinical application prospects.

This invention is a divisional application of patent application number 202311457833.6. This invention belongs to the field of compound synthesis technology, specifically relating to an indole compound that targets and inhibits HDAC6, its preparation method, and its applications. The indole compound that targets and inhibits HDAC6 of this invention is a compound represented by Formula I or a pharmaceutically acceptable salt thereof. Experimental results show that the indole compound that targets and inhibits HDAC6 provided by this invention not only has superior selective inhibitory activity against HDAC6 protein but also inhibits the proliferation of gastric cancer cells in a concentration-dependent manner. Therefore, the compound of this invention has good application prospects in the preparation of inhibitory drugs based on the HDAC6 target and drugs that inhibit the proliferation activity of gastric cancer cells, and is of great significance in the development of anti-tumor drugs and the treatment of related diseases.

The invention belongs to the technical field of biological medicines, and particularly relates to an indole benzamide derivative as well as a preparation method and application thereof. The indole benzamide derivative has broad-spectrum anti-tumor activity, can remarkably inhibit proliferation activity and clone formation of lung cancer cells, prostate cancer cells, breast cancer cells and pancreatic cancer cells, can be used for preparing anti-tumor drugs, and has wide application prospects in the anti-tumor aspect.

The application discloses application of a protein degradation targeting compound to preparation of an antitumor drug. The compound has excellent antitumor cell proliferation activity, and test example data show that multiple compounds have significant concentration-dependent proliferation inhibition effect on colorectal cancer cells. Moreover, the compound has the potential to achieve sustained drug efficacy at a low dose. The compound has good antiproliferation activity on colorectal cancer cells, and therefore has definite potential for preparation of an anticancer drug.

The application belongs to the technical field of medicine, and particularly relates to application of trifluperidol in treatment of cerebrovascular diseases. Trifluperidol is a D1 / D2 dopamine receptor antagonist with oral activity and a novel PI3K inhibitor, and trifluperidol has anti-proliferative activity on cancer cells and induces apoptosis. The application discloses trifluperidol for the first time, and no report is found on effective treatment of trifluperidol against ischemic stroke, especially the application creatively proposes that trifluperidol significantly reduces the volume of acute cerebral infarction of a mouse with focal cerebral ischemia, which indicates that trifluperidol can be used for treating ischemic stroke.