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35results about How to "Avoid photodamage" patented technology

Nano probe for copper ion fluorescence imaging in cells and preparation method for nano probe

The invention relates to a nano probe for copper ion fluorescence imaging in cells and a preparation method for the nano probe. The preparation method comprises the following steps: firstly, synthesizing up-conversion nana particles with core-shell structures, CS-UCNPs for short; then, by taking a CTAB (cetyltrimethyl ammonium bromide) as a template agent and a surfactant, coating a layer of mesoporous SiO2 on the surfaces of the CS-UCNPs to obtain mesoporous coated nano particles CS-UCNPs@mSiO2 with compounding functions; finally, performing covalent bond assembly on functional modified rhodamine organic dye RHB precursors capable of identifying metal copper ions in a singular manner and the CS-UCNPs@mSiO2 to construct an organic-inorganic hybrid nano probe CS-UCNPs@mSiO2-RBH, thereby realizing fluorescence detecting and living cell fluorescence imaging of copper ions in an aqueous solution. The preparation method disclosed by the invention has the advantages of being simple in process, convenient to operate and easy to control structure. The prepared nano fluorescence probe has the characteristics of a uniform dimension, a stable structure, low toxicity and good biocompatibility, and a potential application value in the fields of up-conversion fluorescence labeling, fluorescence detecting and the like of cells or tissues.
Owner:SHANGHAI UNIV

Optical fiber living body fluorescence excitation spectral imaging device

The invention relates to an optical fiber living body fluorescent excitation spectral imaging device which comprises interference modulated light source unit, a microscopic imaging unit, an optical fiber bundle endoscopic unit and a computer, wherein light of the interference modulated light source unit is coupled into the optical fiber bundle endoscopic unit through the microscopic imaging unit, the light output by the optical fiber bundle endoscopic unit irradiates on a sample, the sample emits a fluorescent signal after being irradiated, the fluorescent signal is collected by the optical fiber bundle endoscopic unit and returns and enters the microscopic imaging unit, one end port of the microscopic imaging unit is connected with the computer, and the computer is used for processing the electric signal of the microscopic imaging unit so as to obtain an objective fluorescence excitation spectral imaging device. The optical fiber body fluorescence excitation spectral imaging device has the advantages of being capable of not only providing the form distribution of a sample imaging space, but also providing accurate fluorescence excitation spectral imaging information of each point of the imaging space, achieving simultaneous observation of different biochemical constituents of a biological sample self or varieties of exogenous fluorescent marker excitation spectrums, and providing a novel experimental instrument for the visualization of functional studies of living cells.
Owner:HUAZHONG UNIV OF SCI & TECH

Up-conversion luminescence nanoparticle, and preparation method and application thereof

The invention discloses an up-conversion luminescent nanoparticle and a preparation method and application thereof. The preparation method comprises the steps that core-shell-shell structure nanoparticles are prepared from ytterbium acetate, neodymium acetate, thulium acetate, gadolinium acetate, oleic acid and 1-octadecene through a coprecipitation method, and oleic acid (OA) coated up-conversionluminescent nanoparticles (UCNPs) are prepared. The inert shell structure on the outermost layer can greatly reduce luminescence quenching caused by surface defects of outer-layer ligands and nanocrystals and enhance the up-conversion luminescence intensity. Polyacrylic acid (PAA) is adopted to functionalize the surfaces of the nanoparticles, so that the upconversion nanoparticles have good dispersing capacity in an aqueous solution, and directional recognition of copper ions in an aqueous phase system is realized. The up-conversion luminescence intensity obtained by adopting 980nm or 808nm excitation has a good linear relationship with the copper ion concentration. The nanoparticle has the characteristics of good selectivity, low luminescence detection limit and wide linear range. Ethylene diamine tetraacetic acid (EDTA) is added, so that the luminescence of the up-conversion nanoparticles is recovered, and the up-conversion nanoparticles can be recycled.
Owner:GUANGDONG UNIV OF TECH

Nutritional health-care product for adjusting visual performance

The invention discloses a nutritional health-care product for adjusting visual performance, and in particular relates to a nutritional health-care product for preventing and treating occurrence and development of myopia and cataract. The nutritional health-care product is prepared by a conventional method from the following raw materials in total weight of 100 grams by weight proportion: 1 to 10g of eyeseryl (ocustilla), 0.005 to 0.2g of lutein, 0.001 to 0.05g of vitamin B, 0.04 to 0.15g of vitamin C, 0.005 to 3g of beta-carotene, and the balance of toothpaste raw materials. In the method, the toothpaste is used as a basic raw material, and the eyeseryle (ocustilla), the lutein, the vitamin B, the vitamin C, the beta-carotene and other visual protective trophic factors are added, so a user is ensured to absorb trace visual protective trophic factors required for visual performance adjustment by means of contacting trace eyeseryl (ocustilla) and various vitamins for a long term and absorbing trace eyeseryl (ocustilla) and various vitamins through buccal mucosa in a daily tooth brushing process. Therefore, the occurrence and development of the myopia and the cataract can be prevented, treated and retarded, and meanwhile, clinical information proves that the nutritional health-care product has an excellent treatment effect on canker sores.
Owner:候小滨

Multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system

The invention discloses a multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system which comprises a runway pond. A turbulence carbon dioxide supplement device, an algae cell harvesting device utilizing microbubble back flushing, a flow retaining device and two paddle wheels are disposed in the runway pond, and a groove structure is disposed below each paddle wheel. The multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system is communicated with an algae cell settling pond, a disinfecting pond and a nutritive salt blending pond. The multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system has the advantages that water circulation in the runway pond can be accelerated, and air exchanging frequency between liquid surface and atmosphere is increased; primary concentration of algae cells can be achieved in real time and effectively, and centrifuging efficiency is increased; escaping of dispersed carbon dioxide can be prevented, carbon dioxide utilization rate can be increased, and algae liquid pH can be adjusted fast; convection between the upper layer and the lower layer of the algae liquid can be promoted effectively; continuous microalgae cultivation can be achieved.
Owner:OCEAN UNIV OF CHINA

Cosmetic preparation rich in cell growth factors and preparation method of cosmetic preparation

The invention discloses a cosmetic preparation rich in cell growth factors. The cosmetic preparation is prepared from the following components: 0.01 to 0.2 percent of STP, 0.01 to 0.1 percent of cellgrowth factors, 1 to 10 percent of fish oil, 1 to 10 percent of surfactant, 1 to 15percent of humectant, 0.1 to 1 percent of antioxidant, 0.1 to 3 percent of pH modifier, 3 to 5 percent of emulsifier,0.1 to 5 percent of natural plant extract and deionized water. The invention provides the cosmetic preparation rich in the cell growth factors; the cosmetic preparation has the advantages that aged cells can be constantly replaced with new cells, so that the effects of preventing and removing wrinkles are generated through skin rejuvenation; skin wrinkles, dryness, sagging, aging and light damagecan be prevented, or the texture and smoothness of the skin are improved; the emulsification effect of cosmetics can be enhanced and the stability of cosmetics is improved. The invention also provides the preparation method of the cosmetic preparation rich in the cell growth factors; the preparation method has the advantages of simple operation, simplicity and easiness in obtaining materials, lowcost and worthiness of being popularized.
Owner:上海灰灵生物科技有限公司

Coaxial type axial modulation high-resolution photoacoustic microscopic imaging method and imaging system

The invention discloses a coaxial axial modulation high-resolution photoacoustic microscopic imaging method and an imaging system, belongs to the field of microscopic imaging, and particularly establishes a set of complete coaxial axial modulation high-resolution photoacoustic microscopic imaging system to avoid the problem of poor axial resolution in an orthogonal axial structure light modulation photoacoustic signal scheme. Meanwhile, the invention further provides a set of axial modulation photoacoustic signal method, the axial structure light modulation principle is combined with ultrasonic detection, the limitation that the axial resolution of the PAM system is limited by the detection bandwidth of an ultrasonic probe is broken through, the axial high-resolution PAM system imaging result is obtained, and meanwhile, the defect that light damage exists when the axial resolution of an existing PAM system is improved is overcome. In the method for axially modulating the photoacoustic signal, a coaxial axial structured light implementation scheme is provided on the basis of a double circular seam method, the three-dimensional light field distribution of circular seam masks with different initial phase differences near the rear focal plane of the lens can be easily obtained by changing an input light field, and the calculation is simple.
Owner:HUAZHONG UNIV OF SCI & TECH

A multi-directional turbulent, mixed raceway pond microalgae cultivation system

The invention discloses a multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system which comprises a runway pond. A turbulence carbon dioxide supplement device, an algae cell harvesting device utilizing microbubble back flushing, a flow retaining device and two paddle wheels are disposed in the runway pond, and a groove structure is disposed below each paddle wheel. The multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system is communicated with an algae cell settling pond, a disinfecting pond and a nutritive salt blending pond. The multidirectional-turbulence and efficient-mixing novel runway pond microalgae cultivation system has the advantages that water circulation in the runway pond can be accelerated, and air exchanging frequency between liquid surface and atmosphere is increased; primary concentration of algae cells can be achieved in real time and effectively, and centrifuging efficiency is increased; escaping of dispersed carbon dioxide can be prevented, carbon dioxide utilization rate can be increased, and algae liquid pH can be adjusted fast; convection between the upper layer and the lower layer of the algae liquid can be promoted effectively; continuous microalgae cultivation can be achieved.
Owner:OCEAN UNIV OF CHINA

Preparation method and application of alkanet root extract with stable color

The invention discloses a preparation method of a lithospermum root extract with stable color, which comprises the following steps: mixing lithospermum roots and an ethanol solution with the volume fraction of 50% according to the solid-liquid ratio of 1g: 30mL, and carrying out high-pressure treatment and filtration to obtain a crude lithospermum root extract; uniformly mixing petroleum ether, ethyl acetate, methanol and water according to a volume ratio of (5-7): (3-5): (4-6): (4-6), and standing for layering to prepare a stationary phase and a mobile phase; respectively pumping the stationary phase, the mobile phase and the radix arnebiae crude extract into a high-speed counter-current chromatograph to obtain a high-purity radix arnebiae extract; preparing the high-purity alkanet root extract into alkanet root extract freeze-dried powder; and mixing the freeze-dried powder of the alkanet root extract with octyldodecanol myristate to obtain the alkanet root extract for cosmetics. The preparation method disclosed by the invention has the characteristics of simplifying steps and saving time, and the obtained extract has the characteristics of lasting and stable color, higher active matter purity and better anti-aging effect.
Owner:ZHEJIANG YIGE ENTERPRISE MANAGEMENT GRP CO LTD

A raceway pond microalgae culture system that uses microbubbles to continuously harvest algae cells

The invention discloses a runway pool microalgae culture system using micro-bubble continuous gas flotation to harvest algal cells; the system includes a runway pool, the runway pool is internally provided with a turbulent flow carbon supplementing device, a device using micro-bubble continuous gas flotation to collect the runway pool algal cells, a flow blocking device, a double-paddle-wheel device and a groove structure which is arranged at the lower side. The system has the following advantages: (1) the circulation of a water body in the runway pool can be accelerated, and the gas exchange frequency between a liquid surface and the atmosphere is improved; (2) preliminary concentration on the algal cells can be achieved effectively in real time, the centrifugal efficiency is improved, the centrifugal energy consumption is reduced and the harvesting cost is decreased; (3) distributed carbon dioxide can be prevented from escaping, the utilization rate of carbon dioxide is improved, and the pH of an algal liquid is more rapidly adjusted; (4) exchange between an upper layer and a lower layer of the algae liquid can be effectively promoted, not only can the algae cells on the surface layer be prevented from being damaged by light, but also the total effective light energy quantity received by the algae cells of the unit water body is increased; and (5) the runway pool can realize continuous cultivation of microalgae, and an extremely small amount of waste water generated in the cultivation process can be recycled after the waste water is introduced to a disinfection pool and a nutrient salt blending pool through a pipeline and is treated.
Owner:OCEAN UNIV OF CHINA

Preparation method and application of novel fluorescent nano material with photodynamic therapy and sterilization functions

The invention relates to the technical field of high polymer materials, biological medicines and nano materials, in particular to a preparation method and application of a novel fluorescent nano material with photodynamic therapy and sterilization functions. The preparation method comprises the following steps: dissolving an aniline compound and amino acid with deionized water, adding concentrated sulfuric acid after dissolving, and uniformly stirring and mixing; and transferring the uniformly mixed solution to a polytetrafluoroethylene reaction kettle, and carrying out hydrothermal reaction to obtain the novel macromolecular fluorescent nano material with the spiral structure. The fluorescent nano material can immediately generate hydroxyl free radicals, singlet oxygen and superoxide anion radical free radicals after being illuminated by ultraviolet light, visible light, laser and the like, and has extremely strong oxidation characteristics; when free radicals are generated, body color and fluorescence are changed, the generation of the free radicals can be judged according to the body color and fluorescence characteristics, and according to the characteristics of the photosensitizer, the photosensitizer can be applied to the fields of photodynamic therapy, photodynamic bacteriostasis and the like.
Owner:SOUTH CHINA AGRI UNIV
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