89 results about "Periodontal Membrane" patented technology

A cultured periodontal ligament cell sheet is produced by a process comprising culturing cells on a cell culture support composed of a base material whose surface is coated with a temperature-responsive polymer having an upper limit or lower limit critical dissolution temperature in water of 0 to 80° C. under specified conditions, (1) regulating the temperature of a culture fluid to the upper limit critical dissolution temperature or higher or to the lower limit critical temperature or lower, (2) allowing a cultured periodontal ligament cell sheet resulting from the culturing to adhere to a carrier and (3) detaching the sheet intact together with the carrier. This cultured periodontal ligament cell sheet has a syndesmotic microstructure, exhibits extremely high bioadherence to the dental root surface and realizes high density transplantation of target cells and positive reconstruction of periodontal tissues. Moreover, through lamination of cell sheets to be transplanted and donation of a three-dimensional polarity, highly efficient reconstruction of an adhesion apparatus can be accomplished and clinical application thereof to moderate periodontitis, severe peridontitis, gingival recession and the like is highly promising.

The invention relates to a preparation method of a specific extracellular matrix ECM of periodontium. The preparation method comprises the following steps: 1) separating and culturing a human periodontal ligament cell; and 2) preparing the specific extracellular matrix ECM of periodontium; applying the specific extracellular matrix ECM of periodontium and applying the specific extracellular matrix ECM of periodontium as a human periodontal ligament stem cell in in-vitro amplification; and promoting directional differentiation of the ECM. The preparation method is simple and can remarkably promote in-vitro amplification and directional differentiation of the periodontal ligament stem cell.

The invention discloses stem cells for specifically expressing a programmed death receptor 1 (PD-1), and particularly relates to the stem cells from an organic cavity. The stem cells comprise but notlimited to dental pulp stem cells, gingival mesenchymal steam cells (GMSCs), periodontal ligament stem cells (PDLSCs), stem cells of apical papilla (SCAPs) and dental follicle stem cells (DFSCs) or any combination thereof, and preferably comprise stem cells from human exfoliated deciduous teeth (SHED) and/or dental pulp mesenchymal stem cells for permanent teeth (DPSC); the stem cells also includemesenchymal stem cells from other tissues, which do not express the PD-1 at first and express the PD-1 after being modified by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), suchas PD-1 plus bone mesenchymal stem cells (BMMSC) modified by the CRISPR. The invention further discloses an identifying and separating method for the stem cells for specifically expressing the PD-1,a method for preparing the PD-1 plus mesenchymal stem cells modified by the CRISPR as well as application of the mesenchymal stem cells for expressing the PD-1, disclosed by the invention, in tissue regeneration, pain relief and treatment of chronic pain and a series of diseases.

Disclosed is a bionic dental implant. The bionic dental implant is composed of a titanium substrate, a polyurethane permeating porous titanium dioxide layer and a polyurethane layer which serves as a bionic periodontal membrane, and the polyurethane permeating porous titanium dioxide layer is located between the titanium substrate and the polyurethane layer and respectively and closely integrated with the titanium substrate and the polyurethane layer. A preparation method includes that (1) the titanium substrate is subjected to acid treatment, and then micron grade titanium dioxide powders are deposited layer by layer on the surface of the titanium substrate which is subjected to the acid treatment through an electron beam melting technology to form a porous titanium dioxide layer; (2) isophorone diisocyanate and polyethylene glycol or polyoxytetramethylene glycol or polycaprolactone are used as raw materials to prepare a polyurethane prepolymer; (3) the polyurethane prepolymer is coated on the porous titanium dioxide layer, and then the polyurethane layer is formed under the vacuum condition; and (4) a composite with the polyurethane layer on the surface is soaked in a chain extender solution, a catalyst is added in the solution to react, and after the reaction is finished, the composite is taken out of the chain extender solution and naturally dried by airing in the air.

The invention discloses a comprehensive test experiment platform for an orthodontic parodontium mechanical property, which comprises a base plate, a support, a clamping module and a measuring module; the support and the clamping module are arranged on the base plate; the measuring module is arranged on the upper end of the support; the base plate is provided with a longitudinal guide rail and a horizontal guide rail vertical to the longitudinal guide rail; the support and the measuring module are respectively connected with the longitudinal module in a sliding mode; and the clamping module is connected with the horizontal guide rail in a sliding mode. The clamping module of the test experiment platform provided by the invention can regulate the position in a sliding mode; and the uniaxial drawing/compression and the shearing test of a parodontium test sample can be carried out through regulating the fixture type and the relative position of the clamping module and the measuring module, and the parodontium mechanical performance can be tested under the condition of simulating tooth reshaping. The test accuracy of the device provided by the invention is relative high, and the test accuracy requirement can be met; and the device has less components, is simple in structure, is low in processing cost, and is simple to operate.

The invention provides a preparation method of a novel tissue repair-promoting material and an application thereof, particularly can be used for bone defect and repair treatment, which belong to the field of tissue engineering and regenerative medicine. A composite scaffold is prepared by mixing and freeze-drying TGF-beta3-chitosan-ossein protein microspheres and chitosan sponge containing bioactive human-like collagen protein. The composite scaffold has the characteristics of being good in biocompatibility, is capable of promoting proliferation and adhesion of cells around injured tissues andcollecting stem cells in vivo, and is suitable for cell growth and osteogenic differentiation and the like. Meanwhile, the periodontal ligament stem cells can be tightly combined with the scaffold material, so that the good growth of the cells in the scaffold material is facilitated, and the scaffold material has stronger osteogenic differentiation capacity and can be better applied to treatmentof bone defects. Wide application prospects are realized in the fields of bone tissue engineering and regenerative medicine, particularly in the aspects of bone defect treatment and repair and the like.

The present invention provides a protectant of periodontal membranes for inhibiting the destruction of periodontal tissues, particularly periodontal membranes by participation of Porphyromonas gingivalis. Further, it provides an agent for oral cavity or food and drinks containing the protectant of periodontal membranes having effects of prevention and therapy of diseases involving damage of periodontal membranes.

A protectant of periodontal membranes is proanthocyanidins, particularly proanthocyanidins derived from apple premature fruits or hop bracts having an effect decreasing the damage to periodontal membranes caused by Porphyromonas gingivalis, and an eating and drinking product containing the protectant of periodontal membranes as an effective component.

The invention relates to a method for extracting the three-dimensional tooth root morphology of an intravital tooth based on periodontal membrane imaging anatomical characteristics. The method comprises the steps of performing imaging scanning on a patient, and storing the scanned data to be a DICOM-format body layer data; importing the DICOM-format body layer data of the patient into Mimics19.0 software, and extracting a discontinuously distributed periodontal membrane mask from the image data; enabling the discontinuous distribution to be expanded into continuous distribution; taking a seedmask as a seed region to expand outwards to the root bone boundary, wherein the maximum expansion step does not exceed an artificially set maximum step parameter; and rebuilding a three-dimensional model of the corresponding tooth, and storing and outputting the three-dimensional model in an STL format to complete segmentation and extraction for the intravital tooth. The method is simple and easyto use, and can quickly realize tissue segmentation for the intravital tooth root.

The invention discloses a stress analysis method for a tooth model for simulating wearing of an appliance. The method comprises: S110, collecting intraoral scanning data, and generating a basic orthodontic model; S120, virtually generating a periodontal ligament geometric model on the basic orthodontic model; S130, determining a periodontal ligament constitutive model, and establishing a finite element model of the periodontal ligament; S140, simulating and calculating the movement amount of teeth by using a finite element method, comparing with the tooth movement conditions acquired by experiments, and determining the to-be-determined parameters in the periodontal ligament constitutive model; S150, obtaining a complete orthodontic model; S160, simulating and calculating a stress distribution condition of the teeth after the teeth are worn with a appliance by using a finite element method; and S170, simulating alveolar bone reconstruction and tooth movement conditions after the teeth are stressed according to the tooth stress distribution conditions. According to the invention, a complete orthodontic model containing periodontal ligament is established, and simulation calculation is carried out on teeth after a appliance is worn, so that the process of orthodontic treatment can be simulated on the biomechanical level.

Alveolar bone resorption, periodontal membrane loss, and gingival recession can be inhibited by administering 500 mg to 2000 mg per day of calcium, 10 mg to 40 mg per day of soy isoflavone aglycone, and vitamin D₃ to persons having a tendency for decreased bone density, postmeopausal women, and periodontal disease patients in a maintenance phase; and even an extended period of administration provides a high degree of safety.

The invention discloses a periodontal ligament stress analysis method and device. The method comprises the following steps: 1, obtaining geometric models of a digital tooth model, a digital periodontal ligament model and a digital alveolar bone model; selecting the types of constitutive models of the three models to form a digital dental model; selecting boundary conditions of the digital dental model to obtain a digital dental finite element model and carrying out nonlinear finite element calculation, and obtaining stress distribution data of the digital periodontal ligament model so as to verify the consistency of the digital tooth model with a simulated correction target when the digital tooth model is rearranged. Whether alveolar bone reconstruction is caused by bone resorption or bonedeposition of the alveolar bone under the conditions that alveolar jaw changes in pose and/or bears load can be accurately judged; the constitutive models closer to periodontal ligament characteristics are selected by utilizing the geometric models closer to the actual situation in the mouth of a patient, so that the calculated stress distribution data is closer to the actual situation, and a data basis is provided for design, preparation and inspection of subsequent dental instruments.

The invention discloses a primary separation and culture method for periodontal ligament stem cells, which comprises the following steps: separating periodontal ligaments, carrying out enzymolysis on periodontal ligament tissues by a mixed enzyme of collagenase V of which the mass-volume concentration is 0.05-0.25% and pancreatin of which the mass-volume concentration is 0.05-0.25%, carrying out primary culture on periodontal ligament stem cells, and separating the periodontal ligament stem cells by adopting a limiting dilution method. The primary separation and culture method disclosed by the invention has the following advantages: (1) the cost is reduced because the cost of the pancreatin is lower than that of dispase; (2) the digestion time is shortened, the periodontal ligaments are dense connective tissues, the affinity of the collagenase V to the connective tissues is superior to that of collagenase I, and the collagenase V and the pancreatin are combined, so that the enzymolysis effect is obviously improved.

The invention discloses an absorbable polylactic acid film for enhancing airtightness of an absorbable root canal post of a deciduous tooth. The absorbable polylactic acid film is prepared by dissolving the following raw materials in parts by weight: 0.5-1.5 parts of polylactic acid, and 5-15 parts of solvent. The absorbable polylactic acid film and the absorbable root canal post are made of the same material, and the absorbable polylactic acid film has no influence on human dental follicle cells, deciduous periodontal ligament cells and deciduous dental pulp cells, cannot result in apoptosis of the human dental follicle cells, is nontoxic, can effectively control the effective strength of the absorbable root canal post, can recover functions and a shape of the deciduous tooth for a long time to the greatest extent, and does not influence normal replacement of deciduous permanent teeth. A preparation method of the film is simple and practicable, and can be used for manufacturing during clinical application. The film is used for residual root and residual crown repair of the deciduous tooth, a crown restoration is good in retention postoperatively, and the film meets the clinical requirement of pediatric dentistry.

The invention provides a method for establishing a dental jaw finite element model for orthodontics, which comprises the following steps: step 1, establishing and optimizing model masks of teeth, dental pulp and alveolar bones by utilizing intraoral CBCT three-dimensional data of a human body; step 2, conducting reverse engineering processing on the three-dimensional reconstruction models of the teeth, the dental pulp and the alveolar bone to complete materialization of the models; step 3, generating a personalized periodontal ligament for each tooth, generating an internal three-dimensional solid structure for the alveolar bone, and assembling all part models; step 4, performing mesh generation on the complete dental three-dimensional solid model, and establishing a complete dental jaw mesh model; and step 5, carrying out independent material assignment on each component in the complete dental jaw mesh model to complete the establishment of the three-dimensional personalized dental jaw model finite element model. The dental jaw finite element model established by the method has extremely high geometric similarity and mechanical similarity, is high in modeling speed, and has greatapplication value in the field of orthodontics.

The invention relates to the technical field of bone defect repair, in particular to a periodontal defect repair system containing Let-7a. The system comprises a third cell polymer and hydroxyapatite-beta tricalcium phosphate wrapped in the third cell polymer; the third cell polymer is transfected with let-7a mimic; the let-7a mimic is a double-stranded RNA, and the sequence of a sense strand of the let-7a mimic is as shown in SEQ ID NO. 2. According to the scheme, the technical problem that the effect of repairing bone defects by using periodontal ligament stem cells is poor can be solved. According to the scheme, the osteogenic differentiation capacity of the periodontal ligament stem cells is regulated and controlled by using Let-7a, and a feasible way is provided for rapid and effective repair of periodontal defects in combination with a periodontal ligament stem cell polymer form cost scheme.

The invention relates to a guided tissue regeneration membrane with a bacteriostatic effect and a preparation method of the membrane. The guided tissue regeneration membrane is constructed by using antibacterial drug ornidazole, drug carrier poly(lactic-co-glycolic acid) and film-forming material wool keratin. The method adopts a high-voltage electrostatic spinning method to compound the antibacterial drug ornidazole with the synthetic polymer compound PLGA and the natural ingredient wool keratin, thereby constructing the composite guided tissue regeneration membrane having excellent physicaland chemical properties, a slow release effect on the ornidazole, good cell compatibility and the bacteriostatic effect. The composite membrane can not only block migration of fibroblasts from surrounding tissue into a defect area, and make periodontal ligament cells preferentially migrated, attached, proliferated and differentiated on the tooth root surface, thereby inducing regeneration of periodontal tissue; the composite membrane can play a bacteriostatic role within 7 days after guided tissue regeneration, prevent the occurrence of postoperative infection caused by bacteria, and ensure the effect of tissue regeneration; and the guided tissue regeneration membrane provided by the invention has the simple preparation method, mild film formation conditions, and good cell compatibility and bacteriostatic performance, and meets the requirements of biological in-vivo application, and has good application prospects as a biological membrane with the bacteriostatic effect.

The invention provides an accelerant for proliferation and osteogenic differentiation of a periodontal ligament stem cell, and belongs to the technical field of stem cells. The accelerant discovers for the first time that the expression quantity of non-coding RNA RP11-289F5.1 in an osteogenic differentiation process of the periodontal ligament stem cell is down-regulated. Secondly, the accelerantdesigns and synthesizes the shRNA of silent RP11-289F5.1, and a result proves that the silent RP11-289F5.1 can effectively accelerate the proliferation and the osteogenic differentiation of the periodontal ligament stem cell.

The invention relates to the technical field of cell culture, in particular to a stent-free periodontal ligament stem cell sphere as well as a preparation method and application thereof. The stent-free periodontal ligament stem cell spheres are prepared by utilizing a cell sphere culture plate three-dimensional culture technology and are applied to tissue engineering related to osteogenic differentiation. The stent-free periodontal ligament stem cell sphere disclosed by the invention has good stability and biological activity and relatively strong osteogenic differentiation efficiency, and can be used as a cell bone substitute material in the fields of periodontal tissue engineering and bone tissue engineering or used in combination with a scaffold material to improve the osteogenic efficiency. The stent-free periodontal ligament stem cell spheres cultured and collected by the method are uniform and consistent in size, and the cell activity can be maintained for a long time; and meanwhile, the preparation method disclosed by the invention is repeatable, simple, convenient and feasible, does not need to depend on large-scale equipment or devices, and is beneficial to quickly and effectively obtaining the cell sphere structure.