38results about How to "Promote directed differentiation" patented technology

The invention relates to a preparation method of a specific extracellular matrix ECM of periodontium. The preparation method comprises the following steps: 1) separating and culturing a human periodontal ligament cell; and 2) preparing the specific extracellular matrix ECM of periodontium; applying the specific extracellular matrix ECM of periodontium and applying the specific extracellular matrix ECM of periodontium as a human periodontal ligament stem cell in in-vitro amplification; and promoting directional differentiation of the ECM. The preparation method is simple and can remarkably promote in-vitro amplification and directional differentiation of the periodontal ligament stem cell.

The invention belongs to the field of biomedicine, and relates to a safe and reliable reprogrammed method for obtaining human iPS (induced pluripotent stem) cells, a method for simply, rapidly and efficiently inducing and differentiating iPS cells into spinal motoneurons, and a method for economically and efficiently obtaining functional spinal motoneurons and application of the method. Human fibroblasts are infected with Sendai virus carrying a reprogramming factor by using vitronectin as the sole growth medium, and are cultured for 3 weeks to obtain human iPS cells without exogenous serum interference; the iPS cells are efficiently induced and differentiated into spinal motoneurons by a combination of multiple small molecule compounds; and further, an astrocyte conditioned culture medium promotes maturation culture and is applied in the formation of neuromuscular junctions. The functional spinal motoneurons obtained by efficient induction can be applied to simulation of disease phenotype and discussion of pathogenesis, and provides a theoretical basis for the screening of effective therapeutic drugs in the future, and even in the future can be applied to the cell transplantation therapy of diseases.

The invention relates to the technical field of regenerative medicine and discloses a method for adjusting and controlling in vitro three-dimensional directed differentiation of stem cells. The method includes following steps: embedding Stem cells into calcium alginate micro gel beads containing chondroitin sulfate, adding an inductive differentiation culture liquid, adjusting and controlling the in vitro directed differentiation of the stem cells by changing a mass ratio between the sodium alginate and the chondroitin sulfate during the preparation of the micro gel beads, dissolving the calcium alginate micro gel beads in a sodium citrate solution after the differentiation process being finished, and then obtaining pure differentiation cells. The method is simple in operation and low in cost, the calcium alginate micro gel beads containing chondroitin sulfate can simulate an in vivo three-dimensional extracellular matrix so that a three-dimensional directed differentiating microenvironment which approximates an in vivo microenvironment is supplied for the stem cells, thereby improving directed differentiation efficiency and a biological function of the stem cells. The method plays an important role in applications of regenerative medicine.

The invention discloses the preparation and application of flavonol as a brain-targeting synergist. In a long-term experimental study, the inventor finds that parts of flavonol compounds, particularly kaempferide, rutin, troxerutin, myricetin and aurantiamarin, and the hydroxy ether derivatives of the flavonol compounds, particularly glucoside, ester and ether derivatives of the flavonol compounds, can well promote medicine molecules, such as ginsenoside, stilbeneglycoside, resveratrol, levodopa, edaravone, vinpocetine, nicergoline, citicoline and oxiracetam, which have treatment or healthcare functions on cerebral diseases, to enter brain tissues, so that the concentration of the medicine molecules in the brain tissues is greatly increased without increasing the blood concentration, and the curative effect of medicine is effectively promoted.

The invention relates to a nervous network restoration system and a preparation method thereof. The nervous network restoration system comprises a sustained-release diaphragm, a photosensitive astrocyte adsorbed on the sustained-release diaphragm, and a positively charged conducting polymer dispersed in the sustained-release diaphragm, wherein the sustained-release diaphragm is a hydrogel diaphragm formed by virtue of crosslinking of polyacrylic acid and polyvinyl alcohol. When the nervous network restoration system is used for treating an injured nervous network, the photosensitive astrocyte can specifically increase ATP (Adenosine Triphosphate) release under stimulation of light irradiation, negatively charged ATP is attracted by the positively charged conducting polymer to deposit on the surface of the sustained-release diaphragm, so that release of ATP is realized by diffusion, the defect of transient release of conventional drug treatment is overcome, stem cell proliferation and neuron directional differentiation can be promoted well, and the injured nervous network can be restored effectively.

The invention belongs to the technical field of biotechnology and cell culture, and particularly relates to a method for three-dimensionally culturing bone marrow mesenchymal stem cells based on a Collagel scaffold method. According to the method disclosed by the invention, a Collagel scaffold is used as a three-dimensional matrix, and the bone marrow mesenchymal stem cells and the Collagel are mixed to form cell suspension for culturing, so that a tissue-like physical and spatial three-dimensional structure required by cell growth can be better simulated; the microenvironment of cell growth under different biomechanical effects of an in-vivo physiological environment can be better simulated, the biological functions of growth, proliferation, adhesion, differentiation and the like of the bone marrow mesenchymal stem cells are promoted, and the method is more suitable for researches in the fields of medical tissue engineering and biomechanics, such as intervertebral disc tissue repair and fibrous ring tissue regeneration.

The invention discloses a preparation method of a COL / PEG@CaP biomineralization multilayer film. According to the preparation method, titanium alloy is taken as a substrate, the surface adhesiveness of the titanium alloy is improved with PDA; on the PDA modified titanium alloy substrate, COL and PEG are taken as polyelectrolytes, layer-by-layer self-assembly technology is adopted to prepare a COL / PEG self-assembled multilayer film; and at last, the COL / PEG@CaP biomineralization multilayer film is prepared through biomineralization. The COL / PEG@CaP biomineralization multilayer film prepared bythe invention can effectively improve the biotoxicity of inorganic particles in vivo; the preparation process is simple, low in cost, short in period and suitable for mass production, and has a wide application prospect; the prepared (COL / PEG)@CaP mineralized film has the functions of drug loading and release, osteoblast directional differentiation control and cell differentiation degree detection, and has a wide application prospect in the field of tissue engineering.

The invention discloses a composition based on stem cells and application thereof in preparing preparation for the coronary heart disease. The composition based on the stem cells includes the stem cells prepared through an optimizing method and cell preserving liquid serving as a solvent. Firstly, proper stem cells are selected; secondly, the selected stem cells are further processed through the optimizing method; lastly, the stem cells are collected and resuspended in the cell preserving liquid. A various cell implanting method is adopted in the application of the composition based on the stem cells in preparing the preparation for the coronary heart disease, the time nodes before an operation, during the operation and after the operation are combined, and it is ensured that the transplanted stem cells can give the full play. The optimizing method is used for preparing the stem cells and the stem cell composition in the cell preserving liquid, the ventricle function can be extremely obviously enhanced after cell transplantation, and the left ventricle reconstitution is restrained. The optimal cell composition is provided for treating the coronary heart disease, the source is wide, preparation is simple, toxic and side effects are avoided, and the transplanting effect is good.

The present invention discloses application of bilobalide as a synergist in preparation of a drug or a heath product for preventing cranial nerve injury diseases. An inventor discovers that compounds of bilobalides, particularly bilobalide, bilobalide extracts, and hydroxyl derivatives of bilobalides, particularly glucoside derivatives, ester derivatives and ether derivatives can well promote drug molecules with a treating or health care function on cranial nerve injury diseases to enter brain tissues, wherein the drug molecules comprise ginsenoside, stibene glucoside, resveratrol, levodopa, edaravone, vinpocetine, nicergoline, citicoline, oxiracetam and the like; under the conditions of not increasing drug concentration in blood, the concentration of bilobalide can be increased to a large extent in the brain tissues, so that the efficacy of the drug can be improved, and side effects and adverse reactions of the drug can be reduced.

The invention discloses a stent for directional induction of neural stem cell differentiation and a preparation method thereof, and belongs to the field of polymer materials and biomedical engineering. The adhesion growth of cells is promoted by improving the hydrophilicity of a matrix material of the stent; a microgroove structure is constructed on the inner and outer surfaces of the stent material, and the function of directional induction of the differentiation is achieved; at the same time, the stent can further release biological active factors in the controlled mode and promote the adhesion and the growth of the stem cells after the directional differentiation. The stent has a good application prospect in neural stem cell transplantation.

The invention discloses a production method of a growth factor supported porous biologic ceramic artificial bone scaffold, and belongs to the field of medical science and biomedical engineering. Qualitative and quantitative detection of a cobra venom nerve growth factor (NFG) adsorbed through a biphasic calcium phosphate ceramic (BCP) artificial bone scaffold and artificial bone scaffold function detection prove that the NGF supported BCP is in favor of realizing osteoblast proliferation and directed differentiation. The NGP supported BCP has a good ossification promotion effect, provides a possible method for producing osteoblast in vitro culture scaffolds, and makes researches of bioengineering materials be possible.

The invention discloses application of a piezoelectric material in stem cell proliferation and / or differentiation. The piezoelectric effect of the piezoelectric material is excited through ultrasonicwaves. When the piezoelectric material is applied to stem cell proliferation and / or differentiation, the activity and the dryness of stem cells are not degraded by the piezoelectric material, meanwhile, good biocompatibility can be maintained, and stem cell proliferation can be promoted; and in addition, as the piezoelectric effect of the piezoelectric material is excited through the ultrasonic waves, mechanical signals of the ultrasonic waves can be converted into electric signals provided to the stem cells, and thus directional differentiation of the stem cells can be induced.

The invention provides a method for differentiating natural killer cells from pluripotent stem cells based on single cell sequencing rational design and application of a CSF1R inhibitor, and relates to the technical field of biology. The inventor opens a'black box 'in the process of differentiating the iPSC into the NK according to single cell transcriptome data, provides a rational design method for differentiating the iPSC into the NK, and rationally designs the application of a small-molecule inhibitor in the process of differentiating the NK from the in-vitro pluripotent stem cells. Research finds that the CSF1R inhibitor treatment can improve the NK proportion by inhibiting the differentiation of myeloid cells and shorten the time required for differentiation, so that the CSF1R inhibitor can be used for promoting the differentiation of in-vitro pluripotent stem cells to prepare natural killer cells. The invention provides a preparation method of natural killer cells, which is simple and convenient, and can stably and quickly obtain high-purity natural killer cells.

The invention discloses a stem cell complex for inducing hair regeneration as well as a preparation method and an application of the stem cell complex. The stem cell complex comprises a cell culture scaffold and stem cells, wherein a main body of the cell culture scaffold is a collagen gel hanging drop, and the stem cells are placed in the collagen gel hanging drop; the stem cell complex also comprises an absorbable suture with one end extending out of the surface of collagen gel. In the stem cell complex, the stem cells can be differentiated into a precursor stem cell line similar to organ tissue directionally under the induced differentiation condition, the method simulates the process of the interaction between epithelial cells and mesenchymal cells for hair follicle development, hair with complete functions can be generated after transplantation, and transplantation of the stem cells is more similar to that of tissue organs.

The invention relates to the technical field of biomedical engineering, in particular to a preparation method and application of a tissue engineering material for promoting directional differentiation of mesenchymal stem cells. The preparation method provided by the invention comprises the following steps: transplanting the mesenchymal stem cells to a PLGA (poly(lactic-co-glycolic acid)) stent, by controlling the time, concentration and mode of planting the mesenchymal stem cells as well as the size, the shape and the like of a stent material, searching out optimum conditions for the directional differentiation of the mesenchymal stem cells, and observing biocompatibility of the mesenchymal stem cells and the PLGA stent, change in cellular morphology during inducing, influence of a pancreatic beta cell inducing liquid on cell proliferation as well as acquisition of specific genes, proteins and functions inducing cell differentiation. According to the preparation method disclosed by the invention, the mesenchymal stem cells are planted on the PLGA stent under the optimum conditions, and a pancreatic beta cell, which has high glucose reactivity and is applicable to therapy transplantation, is obtained from culture and differentiation by virtue of the pancreatic beta cell inducing liquid; therefore, the preparation method and application bring hope to the treatment of diabetes.

The invention relates to the technical field of cell culture, and specifically provides a method for obtaining macrophages with phagocytic function through differentiation of pluripotent stem cells. The cell induction medium can realize rapid and large-scale induction of embryoid somatic cells to differentiate into macrophages. Among them, the first six media include serum-free media, which can provide basic nutrients for cell growth, proliferation and differentiation at various stages, and avoid the introduction of exogenous substances to reduce the risk of contamination. The seventh medium contains serum and additionally added FBS, which can well maintain the growth of macrophages. Each medium contains various cytokines which can promote the directional differentiation of cells. The cell induction culture method provided by the present invention is simple, efficient, low in cost, and can be expanded in a large amount in a short period to obtain high-quality and high-purity macrophages, which is of great significance for clinical treatment and research.

The invention belongs to the technical field of a biological technology and cell culture, and particularly relates to a method for three-dimensional culture of fibrous ring derived stem cells based ona Collagel gel bracket method. According to the method disclosed by the invention, a Collagel gel bracket is used as a three-dimensional substrate, and the fibrous ring derived stem cells and Collagel gel are mixed to obtain cell suspension for culture, so that a tissue-like physical and space three-dimensional structure desired by cell growth can be better simulated, the microenvironment of cellgrowth under different biological mechanics actions of in vivo physiological environment can be better simulated, biological functions of growth, proliferation, adhesive attachment, disintegration and the like of the fibrous ring derived stem cells can be promoted, and the method is more suitable for research of the medical tissue project field and the biological mechanics field of intervertebraldisc tissue repair, fibrous ring tissue regeneration and the like.

A person who is used in the field of medicine- Preparation of human cell fusion maternal-fetal blood group incompatibility therapy hybrid strain, characterized by, Using erythropoietin as inducer, ''O'' type human bone marrow cell positive for Rh were cultured, to increase the number of directionally developing erythroid cells, human bone marrow cells and human myeloma cells were fused into hybridized cells, Erythropoietin was use to induce that directional differentiation of the hybridized cell, Hybrid cell clones were screened by HAT, The hybridization strains of Rh positive cells which canbe expanded indefinitely in vitro were screened by Rh antibody, As that adsorbent aft industrialized amplification, A low-cost and safe method for that treatment of maternal-fetal blood group incompatibility hemolytic disease without plasma exchange solution without remove plasma and its beneficial ingredients is create by absorbing pathogenic Rh antibody in peripheral plasma of pregnant women with maternal-fetal blood group incompatibility through an in vitro adsorption device which is formed by an adsorber and a blood separator.

The invention relates to the field of cell engineering, and in particular relates to a culture medium and a culture method for inducing multipotent stem cells for directional differentiation of renal cells. The culture medium for inducing the multipotent stem cells for directional differentiation of the renal cellscomprises 10-15g / L of a DEME basal medium, 0.1-0.5mM of mannitol, 4-8U of heparin sodium, 1-1.5mM of L-glutamine, 0.5-0.85mM of sodium pyruvate, 0.15-0.2mM of beta-mercaptoethanol, 1-3mg / L of fibronectin, 27-39ng / mL of directional differentiation induction factors, and a serum replacement. The culture medium for inducing the multipotent stem cells for directional differentiation of the renal cells, provided by the invention, can effectively promote induction of the directional differentiation from the multipotent stem cells to the renal cells, and no fetal calf serum is added in the culture medium, thereby avoiding a risk of infection of animal pathogens.

The invention provides a nerve conduit loaded with adipose-derived stem cells and a preparation method thereof.The preparation method comprises the steps that S1, polycaprolactone and polyvinylpyrrolidone are added into a binary organic solvent, after ultrasonic treatment, reduced graphene oxide nanoparticles are added, and a spinning solution is obtained; s2, carrying out electrostatic spinning on the spinning solution by utilizing an electrostatic spinning technology, and washing for a plurality of times to obtain a semi-finished conduit product; and S3, injecting the cell mixture into the semi-finished conduit product to obtain the nerve conduit, the surface of the adipose-derived stem cell-loaded nerve conduit fiber provided by the invention has a groove structure, so that the specific surface area and cell adhesion sites are increased, and adhesion and proliferation of cells are facilitated; by loading the adipose-derived stem cells, the neurotrophic phenotypic effect of the peripheral nerve scaffold is improved, and the adipose-derived stem cells can effectively avoid immunological rejection of transplantation and promote directional growth of axons into nerve conduits and the myelination effect of Schwann cells.

An intrabody bioreactor for the tissue-engineered organ transplantation is composed of an external powder pump system and an internal catheter system. It can be used to pump the cell culturing liquid containing the self blood serum of organ receptor, seed cells and growth factors into the transplanted organ and promote its vascularization.

The invention discloses an antibacterial macromolecular polyvinyl alcohol material, a method and an application thereof. The antibacterial macromolecular polyvinyl alcohol material uses a silicon chip as a base material, and prepares a PVA-TP-CA polymer coating on the silicon chip. The polymer coating further introduces Co 2+ Ion, which has the structural formula shown in formula (1). The material of the present invention forms Co 2+ ion‑terpyridine complexes, the terpyridine group provides a platform for the efficient anchoring of bioactive cobalt ions with good control over the introduced Co 2+ The concentration of ions avoids the cytotoxic reaction and protein adsorption induced by high concentrations of metal ions, and ensures that Co 2+ The ions are stably fixed on the surface of the material to avoid being released into liquid matrices such as cell culture fluid. co 2+ The ions can provide long-term antibacterial ability on the surface of the material and improve the mechanical strength of the polymer coating surface, thereby having a physical stimulation effect of promoting the directional differentiation of bone marrow mesenchymal stem cells into bone cells.

The invention relates to preparation of a quality control reference strain for myeloid leukemia fusion gene detection and belongs to the medical field. The preparation is characterized in that a recombinant human granulocyte colony-stimulating factor is introduced and a marrow cell fusion pre-culture step taking the granulocyte colony-stimulating factor as an inducer is added to promote directional differentiation, increment and maturity of cells in stages of grains, so that the fusion rate of the grains is increased. The granulocyte colony-stimulating factor takes part in infusion of marrow cells, screening of hybrid cells and full-course induction of cloning passage amplification thereof, so that differentiation of the cells in stages of grains is facilitated, therefore, the quality control reference strain for myeloid leukemia fusion gene detection amplified indefinitely in vitro obtained by fusing human marrow cells and mouse marrow cells is prepared. Verified by the infusion gene,the strain is applied to quality control for myeloid leukemia fusion gene detection.

The invention relates to the field of cell engineering, and in particular relates to a culture medium and a culture method for inducing multipotent stem cells for directional differentiation of renal cells. The culture medium for inducing the multipotent stem cells for directional differentiation of the renal cellscomprises 10-15g / L of a DEME basal medium, 0.1-0.5mM of mannitol, 4-8U of heparin sodium, 1-1.5mM of L-glutamine, 0.5-0.85mM of sodium pyruvate, 0.15-0.2mM of beta-mercaptoethanol, 1-3mg / L of fibronectin, 27-39ng / mL of directional differentiation induction factors, and a serum replacement. The culture medium for inducing the multipotent stem cells for directional differentiation of the renal cells, provided by the invention, can effectively promote induction of the directional differentiation from the multipotent stem cells to the renal cells, and no fetal calf serum is added in the culture medium, thereby avoiding a risk of infection of animal pathogens.

The invention relates to the field of biotechnology and in particular relates to a PF-127-LV-NFcocktail compound loaded with portable neural stem cells as well as a preparation method and application of the PF-127-LV-NFcocktail compound. The compound comprises Pluronic F-127 hydrogel, a complete neural stem cell culture medium, a Lingo-1 shRNA slow virus expression vector and a nerve growth factor mixture. The preparation method of the PF-127-LV-NFcocktail compound comprises the following steps: 1, dissolving the Pluronic F-127 hydrogel in the complete neural stem cell culture medium; 2, adding the Lingo-1 shRNA slow virus expression vector; 3, adding the nerve growth factor mixture; and 4, performing filtration and sterilization. The compound can load the portable neural stem cells and can promote directional differentiation and nerve regeneration of the neural stem cells.

The invention relates to a method for inducing pluripotent stem cells to be differentiated into melanocytes in a serum-free culture solution, which comprises the following steps: preparing a serum-free matrix culture solution of differentiated cells, including a No.1 culture medium, a No.2 culture medium and a DiffMel culture medium; culturing the pluripotent stem cells in the cell culture plate until the pluripotent stem cells overgrow 80% of the cell culture plate; inducing the pluripotent stem cells to be differentiated into precursor cells of melanocytes in a matrix culture solution; a culture dish for treatment and passage is coated, and precursor cells of the melanocytes are continuously induced to be differentiated into the melanocytes in a DiffMel culture medium; and culturing the differentiated melanocytes. The differentiated melanocytes have cell characteristics similar to those of human primary melanocytes, and have great difference from malignant melanoma cells. Compared with the prior art, the melanocyte differentiation method provided by the invention is short in time consumption and high in differentiation efficiency, and serum or other animal-derived preparations are not needed, so that a basis is provided for future clinical application.

The invention discloses a preparation method of a tissue engineering scaffold with bioactivity. The preparation method comprises the following steps: crosslinking thermally treated soybean proteins by using oxidized sodium alginate under the condition of high speed stirring; and freezing and drying to obtain the soybean protein-based tissue engineering scaffold with bioactivity. The porous bracket prepared by the method has a relatively great bore diameter and porosity and good biocompatibility, has certain bioactivity, can be used for promoting secretion of cellular matrixes and directional differentiation of stem cells and can be used as an ideal carrier of the cell in the tissue engineering field.

A novel PHBV modified induced type bone repair scaffold material is prepared by the steps: dissolving amino acetic acid in a sodium bicarbonate solution, to obtain a first solution; dissolving di-tert-butyl dicarbonate in dioxane, to obtain a second solution; dropwise adding the second solution into the first solution; adjusting the pH value to neutral, dissolving the solids, dropwise adding the filtrate into cold ethyl ether, washing and drying an insoluble substance, and thus obtaining dicarbonate t-butyloxycarboryl-glycine; carrying out condensation on icariin and dicarbonate t-butyloxycarboryl-glycine, adding a mixed liquid of trifluoroacetic acid and dichloromethane into the product, and after the reaction is stopped, evaporating; dissolving the remainder with ethyl acetate; removing the solvent, to obtain aminated icariin; carrying out a condensation reaction; pouring aminated icariin-PHBV into a chitosan solution, injecting into an annular mold, freezing with liquid nitrogen, demoulding, drying, and thus obtaining the novel PHBV modified induced type bone repair scaffold material. The bone repair scaffold material can promote directional differentiation of stem cells into osteoblasts, accelerates bone growth and functional recovery, and can effectively repair bone defects.

The invention discloses the preparation and application of flavonol as a brain targeting synergist. The inventors have found in long-term experimental research that some flavonol compounds, especially kaempferol, rutin, troxerutin, myricetin, hesperidin, and their hydroxy derivatives, especially their glycosides, esters, ethers Derivatives, can be very good to promote the therapeutic drug molecules ginsenoside, stilbene glycoside, resveratrol, levodopa, edaravone, vinpocetine, nicergoline, cytosine When choline, oxiracetam, etc. enter the brain tissue, the concentration of the drug in the brain tissue is greatly increased without increasing its blood concentration, and the curative effect of the drug is effectively improved.

The invention discloses the application of a piezoelectric material in the proliferation and / or differentiation of stem cells, wherein ultrasonic waves are used to excite the piezoelectric effect of the piezoelectric material. When the piezoelectric material is applied to the proliferation and / or differentiation of stem cells, it will not reduce the viability and stemness of the stem cells, but can also maintain good biocompatibility and promote the proliferation of stem cells; and, by using ultrasound to excite the piezoelectric material The piezoelectric effect can convert the mechanical signal of ultrasound into an electrical signal and provide it to stem cells, which is beneficial to induce the directional differentiation of stem cells.