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Application of Piezoelectric Materials in Proliferation and/or Differentiation of Stem Cells

A technology of stem cell differentiation and piezoelectric materials, applied in the application field of piezoelectric materials in stem cell proliferation and/or differentiation, can solve the uncontrollability of mechanical force movement, limit the practical application of piezoelectric materials, and regulate stem cell proliferation and differentiation defects and other issues to achieve good biocompatibility and promote proliferation

Active Publication Date: 2021-06-29
BEIJING INST OF NANOENERGY & NANOSYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the mechanical movement of piezoelectric materials in organisms is uncontrollable, which largely limits the practical application of piezoelectric materials in tissue engineering, and there are still defects in regulating stem cell proliferation and differentiation.

Method used

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  • Application of Piezoelectric Materials in Proliferation and/or Differentiation of Stem Cells
  • Application of Piezoelectric Materials in Proliferation and/or Differentiation of Stem Cells
  • Application of Piezoelectric Materials in Proliferation and/or Differentiation of Stem Cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Using the above preparation Figure 1-3 The barium titanate nanoparticle method prepares the barium titanate nanoparticle, then carries out the following steps:

[0057] Step 1, the stem cells were mixed with 1.3*10 4 The density of each / well was inoculated in a confocal culture dish;

[0058] Step 2, add barium titanate nanoparticles-phosphate buffer solution with a concentration of 50 μg / mL after the stem cells adhere to the wall for 12 hours;

[0059] Step 3, apply 0.8W / cm2 to the stem cells after adding barium titanate for 12 hours 2 Ultrasound 10s;

[0060] Step 4, repeat step 3, once a day, and change the medium every three days for the cells.

Embodiment 2

[0062] In Step 3 of Example 2, barium titanate was added for 12 hours to apply 1.2W / cm2 to the stem cells 2 Ultrasound 10s;

Embodiment 3

[0063] In step 3 of Example 3, 0.8 W / cm2 was applied to the cells after adding barium titanate for 12 hours. 2 Ultrasound 60s.

[0064] Characterize the stem cells of Examples 1-3, it can be observed through a microscope that the barium titanate nanoparticles are swallowed by the cells, the barium titanate around the cells is significantly reduced compared with other areas, and a large amount of barium titanate is gathered near the nucleus and in the cytoskeleton Barium titanate. In addition, it was observed through a microscope that the morphology of cells in Example 2 was destroyed, and the number of apoptotic cells increased; in Example 3, the morphology of cells was obviously destroyed, and the number of apoptotic cells increased significantly. It should be noted that in practical applications, ultrasonic waves will act on stem cells with barriers through human skin or other tissues, which is different from the ultrasonic waves directly acting on cells from the outside of...

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Abstract

The invention discloses the application of a piezoelectric material in the proliferation and / or differentiation of stem cells, wherein ultrasonic waves are used to excite the piezoelectric effect of the piezoelectric material. When the piezoelectric material is applied to the proliferation and / or differentiation of stem cells, it will not reduce the viability and stemness of the stem cells, but can also maintain good biocompatibility and promote the proliferation of stem cells; and, by using ultrasound to excite the piezoelectric material The piezoelectric effect can convert the mechanical signal of ultrasound into an electrical signal and provide it to stem cells, which is beneficial to induce the directional differentiation of stem cells.

Description

technical field [0001] Embodiments of the present invention relate to the field of biomaterials, in particular to the application of piezoelectric materials in proliferation and / or differentiation of stem cells. Background technique [0002] The proliferation and differentiation of stem cells is the core and main goal in the field of tissue engineering and regeneration. Studies have shown that biomaterial-mediated physical factors (such as material hardness, surface topography, and surface charge) and chemical factors (chemical composition) can affect the fate of stem cell differentiation. For example, stem cell-derived neural cells are electroactive cells, and stem cells can undergo neural differentiation based on surface charge. Piezoelectric materials have good biocompatibility and the piezoelectric effect of generating charges under force, and have potential application prospects in the field of stem cell proliferation and differentiation research. [0003] However, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12N5/0797
CPCC12N5/0623C12N5/0662C12N2533/00
Inventor 张晓笛李琳琳其他发明人请求不公开姓名
Owner BEIJING INST OF NANOENERGY & NANOSYST
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