7results about How to "High cytotoxic activity" patented technology

The invention relates to the technical field of NK cell culture, and discloses a serum-free medium for NK cells from umbilical cord blood after cryopreservation and a culture method thereof.The medium comprises a coating solution, an induction medium and an amplification medium; wherein the coating liquid is prepared from an anti-CD16 monoclonal antibody and DPBS (diphenylphosphonic acid); the induction culture medium comprises a basic culture medium, a human-derived platelet lysate and cell factors IL-15, IL-18 and IL-21; the amplification culture medium comprises a basic culture medium, a human-derived platelet lysate and IL-2. The culture method comprises the following steps: obtaining mononuclear cells from fresh umbilical cord blood, and then cryopreserving and resuscitating; and carrying out multiplication culture on the recovered umbilical cord blood mononuclear cells by using the serum-free culture medium. According to the NK cell culture medium, the NK cells with high purity, high killing activity and high cytotoxic activity can be obtained without autoserum culture.

The invention discloses a photosensitizer for endogenous hydrogen sulfide activation and visible light excitation as well as a preparation method and application thereof, and relates to the technical field of medical photosensitizers. According to the photosensitizer provided by the invention, BA and NIS react to obtain a BODIPY compound I2BA substituted by double atoms I, so that the intersystem crossing capability is enhanced, and the yield of active oxygen is increased; a target photosensitizer is obtained by introducing a tetraazobenzene ring Cyclone into the para-position of a benzene ring and combining copper ions Cu < 2 + >. The prepared photosensitizer has good light stability and water solubility, can be activated by endogenous hydrogen sulfide in cancer cells, can generate singlet oxygen (1O2) under the irradiation of green light (520 nm), shows excellent photodynamic activity to tumor cells and low dark toxicity to normal cells, has a high photodynamic activity index (PI value), and can be used as a photosensitizer for cancer cells. The compound is successfully used for photodynamic inhibition and elimination of tumors on HCT116 tumor-bearing mice.

The invention relates to a tetraarylmethane compound with indole and pyrrole frameworks and a synthesis method thereof. The chemical structural formula of the compound is shown as a formula 3. The synthesis method comprises the following steps: adding 2-indolyl carbinol and a pyrrole derivative as reaction raw materials into an organic solvent, stirring and reacting at room temperature under the catalysis of Bronsted acid or Lewis acid in the presence of an additive # imgabs0 # molecular sieve, tracking the reaction by TLC until the reaction is complete, filtering, concentrating and purifying to obtain the 2-indolyl carbinol derivative. According to the tetraarylmethane compound with indole and pyrrole frameworks synthesized by the invention, a biological activity test shows that the derivative has relatively high sensitivity and very strong cytotoxic activity on human liver cancer cells HepG2. According to the method, reaction conditions are relatively conventional, the reaction process is mild, simple and convenient, the cost is low, the method is suitable for industrial large-scale production, and the application range of the method is widened; various substrates are used as reactants, products with various structures are obtained, and the yield is high. # imgabs1 #

A class of novel mubezhiside compounds, their preparation, and applications, including: mubezhiside A and mubezhiside B. This invention removes impurities directly via macroporous lipid column chromatography after boiling in water, simultaneously removing both aqueous and lipid-soluble impurities with high efficiency. Following impurity removal, silica gel column chromatography is used for purification, allowing for significantly larger sample loading and shorter processing time compared to ODS column chromatography. Preparative HPLC is employed for final purification, greatly improving the purification effect.

The invention discloses a 2-acetylglucosamine potassium sulfate salt compound as well as a preparation method and application thereof. The compound has a structure as shown in a formula I. The compound shown in the formula I has biological activity for preventing and / or treating tumors. I

A chimeric antigen receptor includes a target antigen-binding domain, a transmembrane domain, and a signal transduction domain, wherein the transmembrane domain includes at its C-terminus a first polypeptide selected from the group consisting of (1a) a polypeptide consisting of an amino acid sequence in which at least 3 amino acids from the C-terminus are deleted in the amino acid sequence set forth in SEQ ID NO: 1; (1b) a polypeptide consisting of an amino acid sequence having 85% or more sequence identity to the amino acid sequence of the polypeptide of (1a); and (1c) a polypeptide consisting of an amino acid sequence in which one or more amino acids are mutated in the amino acid sequence of the polypeptide of (1a), and a cell expressing the chimeric antigen receptor.

The invention discloses a traditional Chinese medicine compound cell culture fluid and application thereof. The traditional Chinese medicine compound cell culture fluid comprises a basic culture medium, and 1 L of the basic culture medium contains 2-10 mg of notoginsenoside R1, 1-5 mg of notoginsenoside Rb1, 1-5 mg of notoginsenoside Rg1, 0.1-1 mg of notoginsenoside Rg3, 0.5-2 mg of notoginsenoside Re, 1-6 [mu] g of shikonin, 5-15 [mu] g of cordycepin and 1-20 mg of basil polysaccharide. In the traditional Chinese medicine compound cell culture fluid, notoginsenoside Rg3 can be combined with shikonin, so that the cytotoxicity can be enhanced, cord blood NK cells are promoted to be differentiated to mature functional phenotypes with high cytotoxic activity, and the killing efficiency of the NK cells on various tumor target cells is remarkably improved; the notoginsenoside Rb1 is combined with the basil polysaccharide, so that the NK cells can be driven to expand and grow, the activity of the NK cells can be maintained, and the cell purity can be improved, so that the persistence of the cell activity can be enhanced, and the tumor killing rate of the NK cells can be improved.