2results about How to "High cytotoxic activity" patented technology

The invention discloses a traditional Chinese medicine compound cell culture fluid and application thereof. The traditional Chinese medicine compound cell culture fluid comprises a basic culture medium, and 1 L of the basic culture medium contains 2-10 mg of notoginsenoside R1, 1-5 mg of notoginsenoside Rb1, 1-5 mg of notoginsenoside Rg1, 0.1-1 mg of notoginsenoside Rg3, 0.5-2 mg of notoginsenoside Re, 1-6 [mu] g of shikonin, 5-15 [mu] g of cordycepin and 1-20 mg of basil polysaccharide. In the traditional Chinese medicine compound cell culture fluid, notoginsenoside Rg3 can be combined with shikonin, so that the cytotoxicity can be enhanced, cord blood NK cells are promoted to be differentiated to mature functional phenotypes with high cytotoxic activity, and the killing efficiency of the NK cells on various tumor target cells is remarkably improved; the notoginsenoside Rb1 is combined with the basil polysaccharide, so that the NK cells can be driven to expand and grow, the activity of the NK cells can be maintained, and the cell purity can be improved, so that the persistence of the cell activity can be enhanced, and the tumor killing rate of the NK cells can be improved.

A class of novel mubezhiside compounds, their preparation, and applications, including: mubezhiside A and mubezhiside B. This invention removes impurities directly via macroporous lipid column chromatography after boiling in water, simultaneously removing both aqueous and lipid-soluble impurities with high efficiency. Following impurity removal, silica gel column chromatography is used for purification, allowing for significantly larger sample loading and shorter processing time compared to ODS column chromatography. Preparative HPLC is employed for final purification, greatly improving the purification effect.