1969 results about "Stem like cell" patented technology

The invention discloses an intelligent stem cell extraction monitoring method and system based on the Internet of Things, and relates to the technical field of intelligent medical monitoring. The stem cell extraction intelligent monitoring method and system based on the Internet of Things comprises the steps that S1, multi-source data in the stem cell extraction process is collected and preprocessed; s2, evaluating a modal response slope, sudden change delay and disturbance intensity, and dynamically adjusting a sampling strategy; s3, analyzing modal abnormal fluctuation sensitivity, and driving event anchor point judgment and trend identification; s4, judging a multi-source feature coupling state and a stability level, and triggering a visual mode; and S5, identifying trend abnormity, constructing a dynamic baseline, and executing semantic correction and path reconstruction. The core problems of feature mismatching and decision misleading caused by advanced image variation and chemical response delay in the prior art are solved, and the multi-modal fusion quality and the accuracy and stability of joint anomaly detection are remarkably improved.

The invention provides a whole-process intelligent management system for stem cell culture medium development, and belongs to the technical field of intelligent management. According to the method, artificial intelligence, big data analysis and knowledge graph technologies are fused, and the whole process from demand analysis, formula intelligent design and virtual simulation, small-scale culture verification and iterative optimization, pilot scale-up process optimization to quality compliance and tracing is covered. All the modules are coordinated through the central intelligent decision engine, the research and development efficiency of the culture medium can be remarkably improved, the development cost can be greatly reduced, and batch consistency and quality stability of the culture medium are ensured by reducing trial and error and optimizing in real time. Meanwhile, a full-chain electronic tracing function is provided, the requirements of laws and regulations for compliance are comprehensively met, and the problems that traditional research and development are low in efficiency, high in cost, unstable in batch and the like are effectively solved.

The invention relates to the technical field of image processing, and discloses a stem cell microscopic image feature extraction method and system. The method comprises the following steps: carrying out segmentation processing on a stem cell delay 3D confocal microscopic image through a chromatin texture sensitive adaptive threshold segmentation algorithm to obtain a nucleoplasm separation mask image; performing feature extraction processing on the marker fluorescence image according to the mask image to obtain a radial distribution feature vector and a pluripotency maintenance index; performing time sequence quantification processing on the state change sequence based on the index to obtain differentiation track feature data; performing spatial feature extraction processing on the subcellular organelle region through a topological graph construction algorithm to obtain a spatial correlation network graph; and performing feature fusion processing through a multi-layer perceptron fusion algorithm to obtain a stem cell image feature classification result. The accuracy of stem cell state recognition and the reliability of differentiation trajectory prediction are improved.

The invention relates to a medicine, a medicine composition and application of the medicine and the medicine composition in treatment of liver fibrosis. The medicine and the medicine composition comprise an umbilical cord mesenchymal stem cell membrane; the umbilical cord mesenchymal stem cell membrane is prepared by the following steps: coating the surface of a temperature-sensitive culture dish with a matrix; adding the cell suspension of the umbilical cord mesenchymal stem cells into a temperature-sensitive culture dish for culturing; the temperature is reduced, the umbilical cord mesenchymal stem cells and the extracellular matrix secreted by the umbilical cord mesenchymal stem cells are separated in a lamellar mode, and the umbilical cord mesenchymal stem cell membrane is obtained. The medicine and the medicine composition provided by the invention can effectively relieve liver fibrosis.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a skin moisturizing and repairing composition based on dendrobium officinale and fibroin as well as a preparation method and application of the skin moisturizing and repairing composition. Essence extracted from dendrobium officinale tender shoots obtained through a plant stem cell culture technology and stepped molecular weight fibroin prepared through a composite enzymolysis technology serve as raw materials, the safe and efficient composition is developed, and the actual requirements for skin moisturizing and repairing are met.

The invention discloses a stem cell fusion degree detection method and system based on artificial intelligence, and a storage medium. The method comprises the following steps: carrying out image preprocessing on a cell microscope image; automatically calculating an optimal threshold value by using an image threshold value segmentation algorithm to obtain a cytoplasm mask; median filtering is carried out on the original image to reduce noise, then an adaptive threshold segmentation method is adopted, a local threshold is calculated according to local area gray level distribution, and a cell nucleus binary image is generated; performing connected region marking on the cell nucleus binary image, calculating the area attribute of each region, and performing filtering according to a cell nucleus removal ratio parameter to obtain a cell nucleus mask; performing logic OR operation on the cytoplasm mask and the cell nucleus mask to obtain a complete cell segmentation result; and calculating the fusion degree of the stem cells based on the cell segmentation result. Therefore, the problems of accuracy and consistency of judging the fusion degree of the stem cells by observing microscope images with human eyes in the prior art are solved, and accurate detection of the fusion degree of the stem cells is realized.

The invention relates to the technical field of biological products, and discloses a mesenchymal stem cell hair growth liquid and a preparation method thereof, and the mesenchymal stem cell hair growth liquid comprises the following components: hair follicle mesenchymal stem cells; a hair follicle mesenchymal stem cell exosome; growth factors: a vascular endothelial growth factor (VEGF), a fibroblast growth factor (FGFs), a keratinocyte growth factor (KGF-7) and interleukin-10 (IL-10); vitamins: vitamin E, vitamin B5, vitamin B2, vitamin B6, vitamin C and biotin; the plant extracts comprise a cacumen biotae extract, a polygonum multiflorum extract, a ginseng extract, a peppermint extract, a purslane extract and a rhodiola rosea extract; other active ingredients: tripeptide and digoxin; the auxiliary material is a hyaluronic acid-collagen peptide graft copolymer; and the balance of a phosphate buffer solution.

The invention relates to the technical field of biomedicine, in particular to stem cell exosome-loaded bi-crosslinked hydrogel as well as preparation and application thereof, and the bi-crosslinked hydrogel comprises the following components in parts by weight: 50-90 parts of a matrix polymer; 0.1 to 5 parts of an active load; 1 to 10 parts of cyclodextrin; 5 to 20 parts of modified chitosan; 0.01 to 1 part of a photoinitiator; and 0.01 to 0.5 part of growth factors. Through double-network design of physical / chemical primary cross-linking and photo-initiation secondary cross-linking, the bottleneck that the mechanical property and the machinability of the traditional single cross-linking hydrogel are difficult to consider at the same time is overcome; meanwhile, the human umbilical vein endothelial cell exosome is introduced, and efficient loading, uniform dispersion and degradation coupling slow release of the exosome are realized by virtue of beta-cyclodextrin inclusion and a double-crosslinking network. Therefore, the problems of mechanics, degradation, cross-linking speed or tissue adhesion of cross-linked hydrogel in the prior art are solved.

The invention discloses a stem cell preparation for improving function degeneration of prostatitis and a preparation method and application thereof, and relates to the technical field of stem cells, the stem cell preparation is prepared from mesenchymal stem cells UC-MSCs and ADSCs according to the cell concentration ratio of 1: 2, hyaluronic acid hydrogel is used as a delivery carrier, and the stem cell preparation is prepared from the mesenchymal stem cells UC-MSCs and ADSCs according to the cell concentration ratio of 1: 2. And two key auxiliary components of juniperus formosana extract and mulberry extract are introduced. Wherein the optimal combination of the UC-MSCs and the ADSCs significantly enhances the anti-inflammatory ability and the tissue regeneration effect; the hyaluronic acid hydrogel carrier system effectively improves the targeting delivery efficiency of the stem cells and prolongs the action time; the two natural active components of the juniperus formosana extract and the mulberry extract synergistically improve the local microenvironment of prostate and inhibit inflammatory response and fibrosis process. The stem cell preparation developed through a multi-component synergistic effect mechanism has the characteristics of high targeting property, lasting treatment effect, high safety and the like, and a new strategy is provided for clinical treatment of prostatitis.

The invention provides application of enhanced and optimized mesenchymal stem cells and exosomes in anti-aging or senescence delaying, and belongs to the technical field of biological medicine manufacturing. According to the pharmaceutical composition disclosed by the invention, the activity-enhanced mesenchymal stem cells and the exosomes secreted by the activity-enhanced mesenchymal stem cells are applied in a combined manner, so that a remarkable synergistic interaction effect is shown in anti-aging intervention. Experiments prove that the composition can systematically improve oxidative stress injury related to aging, significantly recover antioxidant enzyme activity of serum and brain tissue to be close to a normal level, and effectively reverse cognitive function degeneration, which is represented as behavioral improvement such as shortening of escape latency, improvement of spatial memory ability and the like; the invention is suitable for preparing drugs or functional preparations for delaying body aging or resisting aging, and provides an innovative technical path for developing an efficient and safe anti-aging therapy.

The invention relates to the technical field of cell detection, and particularly discloses a physical detection and analysis device based on stem cell characteristics, the physical detection and analysis device comprises a detection mechanism arranged in an analyzer, the detection mechanism comprises mounting plates, the mounting plates are arranged in an annular array, and the ends, close to each other, of the mounting plates are fixedly connected; according to the stem cell detection liquid preparation device, a test tube is installed in an analyzer through the detection mechanism, so that preparation of the stem cell detection liquid can be completed, and compared with a traditional stem cell detection liquid preparation process, the stem cell detection liquid preparation device has the advantages that the detection efficiency is greatly improved; the test tube needs to be opened for multiple times to extract supernate or add a buffer solution and the like, the operation is simple, the detection and analysis efficiency of the stem cells is improved, stem cell suspension and the like cannot be exposed in an external environment, the damage of the stem cells is reduced, the supernate is conveniently and accurately extracted, and the influence on subsequent detection and analysis use is avoided.

The invention relates to an anti-hair loss and hair growth peptide with alternately arranged D / L amino acids as well as a preparation method and application of the anti-hair loss and hair growth peptide, and belongs to the technical field of biological medicines. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1, D-type and L-type amino acids are alternately arranged and designed, the protease degradation resistance is remarkably enhanced, the in-vitro half-life period is larger than or equal to 48 hours, and beta-catenin protein expression in hair follicle stem cells is activated. The preparation method comprises the following steps: taking Rink Amide resin as a carrier, sequentially coupling D / L type amino acid by adopting an Fmoc solid-phase synthesis method, cracking the resin by trifluoroacetic acid, and purifying by high performance liquid chromatography, so that the yield is more than or equal to 80%, the purity is more than or equal to 96%, and the molecular weight is confirmed to be 956.12 Da by mass spectrometry. The polypeptide can be applied to preparation of anti-hair loss and hair growth cosmetics, medicines and medical instruments, and effectively solves the problems of low stability and insufficient targeting of the polypeptide in the prior art. # imgabs0 #

The invention discloses an epidermal skin model and a construction method and application thereof. The construction method comprises the following steps: performing gas-liquid culture on a transwell membrane on a 3D epidermal organ prepared from epidermal stem cells to prepare an epidermal skin model. The epidermis skin model prepared by the construction method can be rapidly amplified to realize large-scale modeling application in a short time, and the epidermis skin model is complete in differentiation, high in integrity, good in barrier function and beneficial to epidermis-related safety detection or efficacy detection of drugs or cosmetics.

The utility model relates to the technical field of biological tissue engineering, in particular to an organ chip platform integrated with optical tweezers, which is used for assembling and culturing three-dimensional cell tissues and comprises an organ chip with optical transparency and holographic optical tweezers integrated with the organ chip. The organ chip is horizontally arranged and comprises an upper-layer plate, a middle-layer plate and a lower-layer plate which are sequentially stacked, the middle-layer plate is provided with at least two first culture pools used for inducing and differentiating stem cells into different types of cells and at least one second culture pool used for assembling and culturing the different types of cells, and the lower-layer plate is provided with at least two second culture pools used for assembling and culturing the different types of cells. The channel is connected with the first culture pond and the second culture pond; and an objective lens of the holographic optical tweezers faces the top surface or the bottom surface of the organ chip and is used for capturing, moving and assembling cells in the first culture pool or the second culture pool. According to the utility model, the dependence on a micro-pattern processing technology can be reduced, the cost is reduced, and the regulation and control capability on an organoid tissue structure is improved.

The invention discloses an environment-emotion gradient association memristive neural network circuit based on a TAP cell regulation mechanism. The environment-emotion gradient association memristive neural network circuit is composed of a hippocampus module and an amygdala kernel module. The hippocampus module comprises an environment recognition neuron module and an emotion generation neuron module; the environment recognition neuron module and the emotion generation neuron module are each composed of a weight regulation and control module adj based on a TAP cell regulation mechanism, an adder, a memristor, a resistor, an operational amplifier, a function module ABM1 and a sampling holder. Wherein the weight regulation and control module adj is composed of a voltage-controlled switch, a summator, a function module ABM2 and a function module ABM3. The almond kernel module is composed of a weight regulation and control module adj, a memristor, a resistor, an operational amplifier and a sampling holder. The provided circuit simulates the hippocampus and amygdala nucleus of the brain in structure, the regulation principle of TAP cells on neural stem cells is used for reference in a weight regulation mechanism, and bidirectional gradient associated memory of the environment and the emotion can be achieved.

The invention discloses preparation and application of dual-network hydrogel. The dual-network hydrogel comprises the following components: (Z1) a spinal cord acellular matrix network DSM formed by self-assembly of a spinal cord acellular matrix dSECM; and (Z2) a metal-polyphenol network MPN formed by coordination of metal ions and polyphenols; wherein the DSM and the MPN are cross-linked through hydrogen bonds to jointly form a double-network structure. DSM provides a microenvironment suitable for cell survival and growth for the dual-network hydrogel and induces stem cells to differentiate into neurons, and MPN provides the dual-network hydrogel with biological effects of inhibiting inflammation and accumulation of reactive oxygen species (ROS). The dual-network hydrogel has the advantages of the two components, the active component neurotrophic factor and the inhibitor loaded on the dual-network hydrogel can regulate and control immune response after SCI, improve neuroinflammation microenvironment and promote nerve repair, and a new strategy is provided for in-situ tissue repair of SCI.

The present invention belongs to the field of biomedical engineering technology and relates to a bone organoid, its construction method, and its use. The bone organoid is obtained by three-dimensionally culturing a cell mixture comprising stem cells and macrophages. The bone organoid and its construction method of the present invention enable rapid preparation of bone organoids with minimal differences from natural bone, enabling regulation of cell fate and better bone formation and repair.

The invention discloses application of a stem cell exosome in treating depression-like behaviors caused by high-altitude hypoxia, and belongs to the technical field of biomedicine. The stem cell exosome is an exosome derived from human umbilical cord mesenchymal stem cells, and is used for drugs for preventing and / or treating depression-like behaviors caused by high-altitude hypoxia. The exosome can effectively improve depression-like core behavior symptoms caused by high-altitude hypoxia by regulating a brain-intestinal axis; the method starts from remodeling intestinal flora so as to influence the intracerebral metabolite spectrum, and finally plays a role in neuroprotection by regulating and controlling a 5-HT6 / cAMP-PKA key signal channel. In addition, the combination of the hUC-MSC-exo and probiotics shows synergistic interaction potential, and a potential treatment strategy which is novel in mechanism, remarkable in effect and high in safety is provided for the field.

The invention relates to the technical field of cell drug preparation process optimization, in particular to an umbilical cord stem cell drug preparation parameter optimization method utilizing machine learning, which comprises the following steps: preprocessing to generate a control input time sequence and an observation output time sequence, and forming a process instruction library; constructing an intervening state space model based on the two types of time sequences, and assimilating the intervening state space model; defining multiple targets and constraints, compiling a process, simulating on the model, determining a feasible region by combining statistical calibration and formalized verification, and generating a feasibility judgment function; and carrying out microparameterization on the process in the feasible region and optimizing under constraints, carrying out rolling solution according to a preset prediction window in the model prediction control framework, outputting an optimal process flow and a monitoring threshold value, and executing record backflow updating. According to the method, the replicability, the compliance and the cross-batch robustness of the process are enhanced.

The invention discloses a magnesium-containing nanoparticle modified biological scaffold composite material as well as a preparation method and application thereof. The magnesium-containing nano particle modified biological scaffold composite material comprises a biological scaffold material and magnesium-containing nano particles modified on the biological scaffold material, the magnesium-containing nano particles comprise nano magnesium oxide particles and / or nano magnesium oxide-polyphenol compound particles, the nano magnesium oxide-polyphenol compound particles are of a shell-core structure, and the shell-core structure is of a hollow structure. Comprising magnesium oxide serving as a core and a polyphenol compound shell layer coating the surface of the magnesium oxide. The magnesium-containing nanoparticle-modified biological scaffold composite material provided by the invention can prolong the degradation and release of magnesium ions, promote improvement of microenvironment problems such as inflammation and active oxygen outbreak after spinal cord injury, regulate and control oxidative stress and inflammatory response, promote nerve regeneration and motor function recovery, and improve the survival rate of spinal cord injury. The problem that nanoparticles stop in a spinal cord injury area is effectively solved, and support is provided for migration, growth and differentiation of endogenous neural stem cells.

The invention provides a method for producing cell culture meat through 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres, and belongs to the technical field of preparation of cell culture meat. A method for producing cell culture meat by 3D scaffold-free co-culture of pseudosciaena crocea stem cell spheres comprises the following steps: mixing and inoculating pseudosciaena crocea myoblasts and pseudosciaena crocea precursor adipocytes into a cell culture plate subjected to anti-adhesion treatment for proliferation culture, spontaneously gathering to form cell spheres, and carrying out differentiation culture to obtain the cell culture meat. According to the co-culture mode provided by the invention, high-efficiency myogenic differentiation and adipogenic differentiation of myoblasts and precursor adipocytes of the large yellow croakers can be realized through proliferation culture and differentiation culture, and an in-vivo microenvironment can be effectively simulated, so that cultured meat similar to a traditional meat product in texture and flavor is produced.

A composition comprising an expanded population of cells can be used in therapy, wherein the cells have been expanded by a method comprising: i) obtaining an isolated population of HSPCs; ii) culturing the isolated population of HSPCs in the presence of a histone deacetylase inhibitor (HDAC inhibitor) to form a cultured population; iii) adding to the cultured HSPC population an aminothiol compound having the formula RNH (CnH2n) NH (CnH2n) SX wherein R is hydrogen, an aryl group, an acyl group or an alkyl group containing from 1 to 7 carbon atoms, each n having a value of from 2 to 6, and X is H or PO3H2 to form expanded cells; or a pharmaceutically acceptable salt thereof.

The invention provides a stem cell exosome for overexpressing A beta degrading enzyme as well as a preparation method and application of the stem cell exosome. Specifically, the invention provides an application of the stem cell exosome overexpressing A beta degrading enzyme in preparation of drugs, and the drugs are used for at least one of the following: prevention, improvement or treatment of cognitive impairment related diseases; neuronal cell damage is improved; wherein the stem cells for overexpressing the A beta degrading enzyme are prepared through lentivirus transfection. According to the stem cell exosome overexpressing the A beta degrading enzyme, the content of the A beta degrading enzyme is obviously higher than that of the exosome prepared by a traditional method. Therefore, when the compound is prepared into a medicine for treating or preventing cognitive impairment related diseases, the medicine can be efficiently conveyed to the brain, and the concentration of the medicine in the brain is remarkably improved, so that the curative effect is enhanced, the dosage of the medicine can be reduced, the side effect is reduced, and the prognosis of brain diseases is effectively improved.

The invention discloses an engineered exosome for promoting hair regeneration and a preparation method and application thereof, Wnt10b gene is inserted into a plasmid vector to construct a target plasmid, the target plasmid and a lentivirus packaging plasmid are co-transfected to packaging cells to generate lentivirus particles, and then target stem cells are infected. Screening to obtain a cell strain capable of stably expressing a target gene, culturing the stably transfected strain, and collecting the engineered exosome generated by the stably transfected strain. The exosomes are rich in Wnt10b protein and can promote proliferation and differentiation of hair follicle stem cells on a molecular level, so that hair follicle tissues in a resting period are activated and enter a growing period again, and hair regeneration is realized. Meanwhile, the exosome is high in safety, and adverse reactions caused by traditional chemical synthesis drugs are avoided. The exosome can be applied to the preparation of hair regeneration products, and the accurate symptomatic treatment capability of the exosome provides a hair growth method aiming at pathogenesis for alopecia patients, and provides a new hope for patients who seek safe and effective hair growth solutions.

The utility model discloses a stem cell storage tank convenient for automatic filling of liquid nitrogen, which relates to the technical field of stem cell storage and comprises a tank body, supporting legs, a locking mechanism and an automatic filling component. The supporting legs are fixedly arranged at the bottom of the tank body; the top of the tank body is rotationally connected with a top cover; the locking mechanism is fixedly arranged on the top of the top cover and used for rapidly locking the top cover. According to the liquid level meter, the position of the floating ball on the liquid level meter is changed along with the change of the liquid level, and when the floating ball drops to the bottommost part, the control panel can send out an alarm signal. And then, the low-temperature electromagnetic valve is automatically opened, and liquid nitrogen flows into the tank body through the liquid nitrogen valve to be supplied. And meanwhile, a temperature sensor arranged in the device continuously monitors the temperature in the tank body. According to the system, the filling process of liquid nitrogen can be automatically regulated and controlled, the burden of operators is effectively relieved, timely and accurate liquid nitrogen supply is ensured, and the overall operation efficiency and safety are improved.

The invention discloses a perfusion device for stem cell culture, and belongs to the technical field of cell culture.The perfusion device comprises a frame, a closing assembly convenient to overhaul is arranged on the frame, a culture container is arranged in the frame, a perfusion bottle is fixedly installed in the frame, a filter box is fixedly installed in the frame, and the filter box is connected with the perfusion bottle. A pumping assembly is arranged in the frame, a penetrating groove is formed in the top of the filter box, and a connecting pipe is connected to the filter box; culture cells are conveniently collected through the arranged filter cylinder, the cells cultured in the perfusion bottle and a culture solution flow into the filter box through a liquid supply pipe, the filter cylinder is covered with a filter membrane, the liquid can flow to the outside to intercept the cells, then the cells can be filtered in the filter cylinder to be collected, then the filter cylinder is rotated, and the cells can be conveniently collected. The threaded sleeve is separated from the threaded head, so that the filter cartridge can be detached, the cells can be conveniently separated and collected, and the working efficiency is improved.

The invention provides a culture method, application and detection method of mesenchymal stem cells for treating nervous system diseases, and belongs to the technical field of biological medicines. The invention provides a culture medium of mesenchymal stem cells, a method for culturing and subculturing the mesenchymal stem cells by using the culture medium, and a method for detecting the biological efficacy of the mesenchymal stem cells for treating nervous system diseases. The mesenchymal stem cell preparation disclosed by the invention has a relatively good treatment effect on nervous system diseases, and can be used for improving the new object recognition capability and the cerebral infarction range, inhibiting the release of inflammatory factors, reducing the oxidative stress level and narrowing the spinal cord lesion range so as to improve nervous system injury.

The invention discloses a preparation method of a stem cell exosome chitosan compound covered stent. The stem cell exosome chitosan compound comprises a stem cell exosome and a chitosan compound. In the stem cell exosome and chitosan compound, the mass percent of chitosan is 3%-10%, and the number of the stem cell exosomes is 109-1011. Compared with a traditional covered stent, the covered material provided by the invention can resist inflammation, inhibit bacteria, protect vascular endothelial cells covered by the stent, promote tissue repair, prevent tissue adhesion, prevent fibrosis and inhibit exclusion reaction and autoimmunity.

The invention provides a mesenchymal stem cell confluence degree calculation method and system based on image semantic segmentation, and the method comprises the steps: training a cell image semantic segmentation model through a training set, and carrying out the test screening of the trained cell image semantic segmentation model through a test set, obtaining an optimal cell image semantic segmentation model; performing preprocessing operation including scale transformation and gray level normalization on the to-be-calculated mesenchymal stem cell phase contrast microimage; and segmenting the to-be-calculated mesenchymal stem cell phase contrast microscopic image into cells and a background by using the optimal cell image semantic segmentation model, and summarizing and counting the pixel proportions of the segmented cells to obtain the mesenchymal stem cell confluence degree so as to realize automatic identification and separation of the mesenchymal stem cells. The method is used for assisting cell culture quality evaluation in medical and biological research.

The invention relates to the technical field of biological medicines and ophthalmic medicines, in particular to a herbal exosome composition for eye care and treatment and a preparation method thereof. The composition comprises a synergistic combination of herbal exosomes and mesenchymal stem cell exosomes, and further comprises a nano slow-release carrier system. Wherein the herbal exosome is extracted from a traditional herbal plant liquorice by combining gradient centrifugation with a molecular exclusion chromatography method, and herbal active ingredients, namely astragaloside and quercetin, are loaded in the exosome by adopting an electroporation method, so that the pharmacological activity of the exosome is enhanced. The nano slow-release carrier system is of a two-phase slow-release structure; an inner phase is a phospholipid complex loaded with herbal exosomes and mesenchymal stem cell exosomes, and an outer phase is temperature-sensitive hydrogel; the compound is in a liquid state at room temperature, and is rapidly converted into a gel state after being dropped into the ocular surface, so that the retention time of the medicine on the ocular surface is remarkably prolonged, the bioavailability is improved, and the targeting slow release capability aiming at ocular surface tissues is endowed to the compound.