241 results about "Stem like cell" patented technology

The present application relates to the field of plant tissue culture technology, and particularly discloses a lily stem cell abundance ex vivo regulation method. The lily bulb is used as the starting material, stem cell magnitude amplification and regulation culture can produce lily tissue organ materials with high stem cell abundance. The present application provides sterile lily materials with high stem cell abundance, which has the characteristics of strong regenerative ability, high proliferation rate, etc., is the basis for protoplast preparation and high-efficiency regeneration, and is suitable for different lily varieties, and provides technical support for cell engineering breeding, seedling scale breeding, etc.

This invention provides novel stem cell activators, topical skin agents containing the aforementioned stem cell activators, and skin cosmetics containing the aforementioned stem cell activators. The stem cell activator of this invention comprises at least one compound selected from compounds (including optically active agents) shown in formula (1), salts of the aforementioned compounds, and hydrates thereof as active ingredients. In the following formula, R... 1 R 2 Represents a hydrogen atom or a hydrocarbon group. R 3 Represents a hydrogen atom or an organic group. n represents an integer greater than or equal to 1. The above hydrocarbon groups may have substituents. [Chemical Formula 1]

It refers to a composition for promoting hair growth, which comprises isolated and expanded MSCs, preferably adipose derived stem cells, ATP and a pharmaceutically acceptable vehicle. It further refers to a kit of parts comprising individual vials with MDCs and ATP. It refers also to said composition or said kit for use in androgenic alopecia. It further refers to cosmetic or non-therapeutic use of said composition or said kit for promoting hair growth. It further refers to isolated and expanded MSCs, preferably adipose derived stem cells, for use in promoting hair growth and for the prevention and / or treatment of androgenic alopecia.

This invention belongs to the field of biomedical materials technology, and particularly relates to an analgesic microneedle patch, its preparation method, and its application. The analgesic microneedle patch of this invention uses extracted mesenchymal stem cell exosomes and ginger exosomes as analgesic active substances, and by combining them with microneedle patch technology, it provides a new technical approach for surface anesthesia. The analgesic microneedle patch of this invention combines microneedle technology and photopolymerization 3D printing technology to develop a customizable hydrogel microneedle patch, achieving minimally invasive and precise percutaneous delivery of local anesthetics, providing a new technical approach for surface anesthesia.

This invention discloses a low-energy laser pretreatment method for enhancing the paracrine function of mesenchymal stem cells. Specifically, the method includes irradiating human umbilical cord mesenchymal stem cells with an 808nm laser. The irradiation parameters are: output power density, irradiation time of 60-120 s per irradiation, continuous irradiation of cells for 3 days, and irradiation twice a day.

The application provides a preparation method of a multi-layer sandwich type epoxy group modified magnetic microsphere and application thereof. The multi-layer sandwich type epoxy magnetic microsphere prepared by the preparation method provided in the application can realize efficient and accurate cell sorting through coupling of an antibody, is suitable for cell sorting related scenes such as stem cell separation, tumor cell detection, immune cell treatment (such as CAR-T cell preparation) and pathogenic microorganism enrichment, and provides key material support for cell sorting technology in life science research and clinical diagnosis and treatment.

The integration procedure provides different elements that require in vitro amplification of HSPCs, preferably for about 48 hours, in the presence of hematopoietic cytokines as well as UM171 and stem cell regenerative factor 1, and the introduction of i) a system (e.g., a CRISPR / Cas9 system) that generates DNA double-strand breaks (DSBs) upstream of the transcription start site of the target gene, and ii) the introduction of a donor matrix comprising cDNA with homologous arms on both sides (left [LHA] and right [RHA]).

The utility model provides a kind of hematopoietic stem cell liquid nitrogen transfer tank, it is related to the technical field of liquid nitrogen transfer tank.The hematopoietic stem cell liquid nitrogen transfer tank includes tank body, and the bottom surface of the tank body is fixedly connected with output port.The hematopoietic stem cell liquid nitrogen transfer tank is matched between output port, storage tank, gas outlet pipe and storage bag, when liquid nitrogen evaporates, the pressure in the tank body is increased, the moving plate will be moved downward, spring is compressed, telescopic rod is shortened, the moving plate will be separated from the screw pipe, gas enters the inside of storage tank, then enters storage bag through gas outlet pipe, nitrogen is stored, the pressure in the tank body is reduced, the problem that the liquid nitrogen transfer tank needs to be frequently opened and closed during use to take out or add hematopoietic stem cells is solved, a part of liquid nitrogen will evaporate and expand when tank cover is opened each time, if the gas cannot be effectively discharged, the pressure in the tank body may be sharply increased in a short time.

The application provides a kind of basic phosphatase induced T1 and T2 signal switching response type magnetic resonance imaging contrast agent and its preparation method and application, the response type magnetic resonance imaging contrast agent includes superparamagnetic nanoparticle, and polyethylene glycol phosphoric acid monoester modified to the surface of superparamagnetic nanoparticle;The superparamagnetic nanoparticle includes ferroferric oxide nanoparticle.The contrast agent of the application can realize T1 and T2 signal switching, is used for stem cell osteogenic differentiation monitoring, not only improves the detection sensitivity of magnetic resonance imaging to microstructure and function, also provides new tools and methods for stem cell treatment in biomedical research and clinical practice.

The application discloses a kind of mesenchymal stem cell ball fractional dissociation digestive juice, its preparation method and application, it is related to cell preparation and three-dimensional cell culture technical field, specifically at to provide C liquid, D liquid and E liquid three kinds of gradient concentration working solution.When dissociating, the mesenchymal stem cell ball to be dissociated is determined its average particle size;According to the average particle size, select C liquid, D liquid or E liquid as digestive juice as described above;The selected digestive juice is mixed with the mesenchymal stem cell ball, and digestion reaction is carried out under oscillation condition, and single cell suspension is obtained.The application establishes the hierarchical matching rule of mesenchymal stem cell ball particle size and specific digestive juice concentration, and optimizes oscillation digestion condition, so as to maintain high activity rate and complete phenotype characteristics while efficiently dissociating, realizes the standardization of process and the controllable of dissociation quality, provides stable and reliable solution for the large-scale preparation and quality control of mesenchymal stem cell ball.

Provided are methods relating to SIRPgamma as a biomarker for cancer cells, and in particular of cancer stem cells and lung adenocarcinoma. Disclosed are also methods of treatment, which involve administering a SIRPgamma targeted agent to a subject with cancer, alone or with another cancer therapy. Methods for diagnosing cancer, identifying subjects with high SIRPgamma levels for treatment, and monitoring SIRPgamma expressing tumors are also provided.

A reagent, a drug source material, a drug, a quasi-drug, a cosmetic source material, or a cosmetic that includes a supernatant of a culture solution of stem cells and is for reducing skin redness or inflammation, inflammation, number of pores, pore size, skin spots, and sebum. A reagent, a drug source material, a drug, a quasi-drug, a cosmetic source material, or a cosmetic that includes a secretion of stem cells and is for reducing skin redness or inflammation, inflammation, number of pores, pore size, skin spots, and sebum. The secretion of the stem cells may be extracellular vesicles. The secretion of the stem cells may be exosomes.

The application provides a stem cell collector, which comprises a body and two extrusion devices, the body has two inner cavities, a front side wall of the body is formed with two extrusion surfaces, the extrusion surfaces are provided with openings in communication with the inner cavities, and the two extrusion devices are arranged on the body and correspond to one extrusion surface respectively. The extrusion device comprises an extrusion plate, a first moving assembly, a partition plate, a second moving assembly, an extrusion block, a third moving assembly and a driving mechanism. The stem cell collector can automatically extrude a blood bag, facilitates the collection of a solution containing a plasma layer, a white blood cell and platelet layer and a small part of a red blood cell layer, reduces the mixing of the red blood cell layer, improves the collection efficiency and brings convenience for the collection of stem cells.

The utility model relates to stem cell collection technical field, concretely disclose a kind of collection device for subtotipotent stem cell, including filter cartridge, the bottom of filter cartridge is fixedly installed with closure, the top of filter cartridge is fixedly installed with input pipe, the bottom of filter cartridge is fixedly installed with discharge pipe, the outside of discharge pipe is provided with sterile collection bottle, the inside of filter cartridge is provided with filter assembly, the top of input pipe is provided with fixed dismounting assembly, the top of fixed dismounting assembly is provided with connecting pipe, the top of connecting pipe is fixedly installed with collection bag, this is used for the collection device of subtotipotent stem cell, flange bolt connection and pipe clamp sealing design are used in fixed dismounting assembly, both through rigid connection ensure pipeline stable, and sterile environment is formed using sealing washer to prevent pollution, and support quick dismounting replacement collection bag, and give consideration to sealing property, convenience and reuse requirement.

The present invention relates to: a stem cell isolation kit manufacturing composition prepared with graphene dispersed therein, and a preparation method therefor, the composition enabling cell activity of stem cells to improve and blood clots to be prevented through mixing of graphene and a thermoplastic resin. The stem cell isolation kit manufactured using the stem cell isolation kit manufacturing composition comprising graphene, of the present invention, allows autologous stem cells to be activated by an environment in which biocompatible far-infrared (FIR) emission and electrical conductivity are increased, and, under hypoxic conditions, enables stem cell water amount to be increased through low oxygen permeability (water activity). In addition, the present invention is subjected to antibacterial treatment so as to provide an antibacterial effect, and can improve a platelet adsorption reduction rate through a water slip effect of the graphene composition.

The present invention relates to a single-phase, spherical edible microcarrier for the three-dimensional culture of stem cells intended for the preparation of cultured meat. The microcarrier comprises a homogeneous mixture of edible ionic polysaccharide (0.375-1.5%), edible protein (1-2.5%), edible polymer (0.025-0.1%) and water (95.9-98.6%). The invention also relates to a method for preparing these microcarriers by mixing three solutions: ionic polysaccharide in saline solution, edible protein in saline solution and edible polymer in water. The mixture is subjected to a process involving microspraying or electrospraying with calcium chloride in order to form spherical microcarriers of 50-300 micrometres. The design of the single-phase, spherical edible microcarrier simplifies the production of cultured meat by dispensing with the need for core-shell structures, thereby improving cell anchorage efficiency and facilitating cell recovery.

The application discloses a neurodegenerative disease treatment composition and relates to the technical field of neurodegenerative disease treatment.The active ingredients of the composition include: exosomes secreted by neural stem cells, exosomes from ginseng, and exosomes from intestinal probiotics; wherein: the three kinds of exosomes exist in parallel, and are all formed by the common induction of traditional Chinese medicine active substance ginseng exosomes and intestinal probiotic fermentation liquor; after the neural stem cell exosomes are induced by the common induction of ginseng exosomes and intestinal probiotic fermentation liquor, the neurotrophic factors and regulatory RNA carried by the neural stem cell exosomes are both enhanced in content and functional activity, so that the neural repair and regulation capabilities are improved. The neural stem cell exosomes are induced by the common induction of ginseng exosomes and probiotic fermentation liquor, the neural repair, anti-inflammatory and antioxidant, and brain-gut axis regulation capabilities of the neural stem cell exosomes are enhanced. When the combined drug or stem cells are applied, the treatment effect and cell integration efficiency are improved, and a new scheme of synergistic precise treatment is provided for neurodegenerative diseases.

The invention discloses a separation device for human stem cell culture, and relates to the technical field of stem cell culture, the separation device comprises a case, a hinge is fixedly arranged on the upper surface of the case, and a cover plate is fixedly arranged at the movable end of the hinge. By arranging the machine box, a closed and clean operation cavity is provided for the whole stem cell separation device, pollution of the external environment to a culture sample is effectively isolated, and meanwhile the machine box serves as an integrated bearing platform of all functional parts. The upper cover structure capable of being opened and closed is formed by arranging the hinge and the cover plate, and the temperature adjusting mechanism is used for adjusting the temperature of the test tube. The temperature adjusting mechanism is arranged and is a key system for ensuring that a suitable temperature environment is maintained in the separation operation process of a stem cell culture sample, and a stable and uniform local temperature field is provided for each test tube through active heating and directional airflow conveying. The effect of keeping the temperature of the test tube stable during stem cell separation is achieved.

The application relates to the field of biological medicine technology and discloses normal-temperature preservation of stem cell exosomes in skin-care composition application, which comprises the following steps: obtaining a secondary drying time according to sample temperature and shelf temperature; obtaining a moisture step degree according to the difference of residual moisture content of a pressure increasing rate; obtaining a moisture continuous removal index according to the residual moisture content, combining the moisture step degree to obtain a comprehensive moisture removal efficiency; obtaining an activity loss degree according to protein activity and the comprehensive moisture removal efficiency, and then obtaining a water content critical threshold value to construct a damage sensitivity; obtaining a damage index according to the protein activity, the comprehensive moisture removal efficiency and the damage sensitivity, and selecting a target pressure increasing rate; and performing stem cell exosome production application in skin-care composition according to the secondary drying time, the water content critical threshold value and the target pressure increasing rate. The application prolongs the normal-temperature preservation duration of stem cell exosomes in skin-care composition application by regulating the secondary drying process.

The present invention relates to a composition for preventing, alleviating, or treating neuronal aging-related diseases or disorders, the composition comprising extracellular vesicles derived from three-dimensional spheroid-type cell aggregates. The extracellular vesicles according to the present invention can reduce the expression of aging-related proteins and induce reactivation of aged neural stem cells, and thus can be used to treat various neuronal aging-related diseases or disorders by preventing, alleviating, or treating the aging of neurons.

The application discloses a method for differentiating stem cells into caudal serotonergic neurons, a complete culture medium and application. Specifically, a new culture medium is formed by adding small molecule compounds, so that human pluripotent stem cells are gradually induced to obtain hindbrain neural stem cells, ventral hindbrain caudal neural stem cells, serotonergic precursor cells and caudal serotonergic neurons of the hindbrain. The culture method is simple, and can efficiently obtain mature serotonergic neurons of specific regions, thereby providing an effective cell model for research on diseases related to the serotonergic system.

This invention provides a treatment system for promoting dental pulp repair using oral stem cells, belonging to the field of oral treatment technology. The system utilizes a dynamic gene regulation module, an adaptive intelligent scaffold, and a bio-digital fusion interface in synergy. Through the dynamic gene regulation module and the adaptive intelligent scaffold, the efficiency and therapeutic effect of dental pulp regeneration are significantly improved. The dynamic gene regulation module employs a light-controlled CRISPR system to perform real-time gene editing on dental pulp stem cells, dynamically adjusting according to the needs of the regeneration stage. The adaptive intelligent scaffold, based on 3D printing technology and manufactured using a digital model of the patient's teeth, can dynamically release growth factors and optimize the microenvironment. This combination of personalized design and dynamic regulation allows for adaptive adjustments to meet the specific needs of the patient, not only improving the repair efficiency of dental pulp tissue but also enhancing the success rate of functional reconstruction. This provides patients with dental pulp injury with a more efficient and precise treatment option, promoting the personalized development of oral regenerative medicine.

This invention provides a pH-responsive immunomodulatory microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. This invention incorporates Zif-8 nanoparticles loaded with ChABC into GelMA hydrogel to prepare a pH-responsive immunomodulatory microneedle patch (Z@C-MN). As the patch gradually degrades, the system can continuously release Zn in a pH-responsive manner. 2+ Z@C-MN, along with ChABC, promotes in situ repair of cerebral fibrosis (SCI) after injury. Z@C-MN induces macrophage polarization towards a pro-regenerative phenotype, thereby downregulating the inflammatory response, increasing neurite length, and promoting the differentiation of neural stem cells into neurons. In vivo, Z@C-MN reduced the inflammatory microenvironment after injury and promoted angiogenesis in a rat SCI model. Furthermore, it activated axonal regeneration by inhibiting scar tissue formation and regulating CD4+. + T cell transformation supports neurogenesis.

The object of this invention is to provide novel pharmaceutical agents that can be used for various treatments. The pharmacological compositions of this invention are characterized by comprising extracellular vesicles derived from equine mesenchymal stem cells. The pharmacological compositions of this invention are tissue repair agents, anti-inflammatory agents, angiogenic agents, or wound healing agents.

The present disclosure relates to compounds for treating cancer. The compounds may prevent conversion of non-stem cancer cells into cancer-initiating cells caused by radiation treatment.

1. Name of the design product: automatic cell recovery instrument (multi-station waterless dry type). 2. Use of the design product: used for the thawing and recovery of biological samples such as blood products, organoids, stem cells, CAR-T cells, etc. 3. Design points of the design product: in shape. 4. Picture or photo that best indicates the design points: perspective view.

A silicon-anchored hardware enforcement system enables low-latency, cryptographically gated execution of artificial intelligence for stem cell therapy and regenerative medicine. A Sovereign Identity Token derived from a Physical Unclonable Function (PUF) permanently binds model decryption to a specific hardware instance and restricts execution to a Trusted Execution Environment synchronized to a hardware-protected Safety Epoch. Encrypted AI model weights are decryptable only upon successful hardware validation. Multimodal clinical inputs, including genetic, imaging, and structured patient data, are integrated through a hardware-constrained fusion architecture that operates under enforced biological safety parameters. FPGA-implemented predicate logic evaluates defined biological safety conditions at sub-millisecond latency, and an ASIC-based nullification circuit irreversibly suppresses outputs that violate hardware-defined thresholds within a bounded millisecond response time. A distributed revocation protocol propagates credential invalidation across networked nodes within sub-second latency while preserving reduced-capacity safe mode operation. A permissioned provenance ledger records hardware-attested execution events and supports automated regulatory documentation. The system provides secure clinical deployment, federated research enablement, and verifiable auditability for high-risk therapeutic environments.

The present application relates to the technical field of biology, and particularly relates to a use of a cell preservation solution in preparation of an immunosuppressant. The present application provides a use of a cell preservation solution in preparation of an immunosuppressant, the cell preservation solution is obtained by a specific method, and the cell preservation solution is applied to a preparation (such as an injection solution) containing mesenchymal stem cells, so that the injection solution is endowed with certain immunosuppressive function, the combination with MSCs cells improves the treatment effect, and the long-time transportation capacity and the survival rate of MSCs are improved.