1042 results about "Stem like cell" patented technology

Methods for propagating haploid genomes of male or female origina and genetic screening and modification thereof are provided. These haploid genomes may be used to produce haploid embryos, and embryonic stem-like cells and differentiated cells. Also, these haploid genomes and cells containing, may be used as nuclear transfer donors to produce diploid nuclear transfer units. These diploid NT units e.g., human NT units, may be used to obtain pluripotent cells and differentiated cells and tissues.

The invention provides an application of a human umbilical cord mesenchymal stem cell complex cell factor in preparing a biological agent for repairing skin injury. The skin injury includes laser burn, thermal burn, cold injury or common trauma skin physical and chemical injury. According to the application, an umbilical cord stem cell complex growth factor is firstly used for the treatment of human skin laser injury; the human umbilical cord mesenchymal stem cell complex cell factor can be preserved in maximal activity and plays a maximal role of injury repair and treatment under a certain preservation condition.

Populations of stem cells and methods for their isolation and use are provided. These stem cell populations comprise aldehyde dehydrogenase positive (ALDH^br) cells isolated from bone marrow, and ALDH^br CD105⁺ cells derived from any stem cell source. These populations may also comprise cells expressing such surface markers as CD34, CD38, CD41, CD45, CD105, CD133, CD135, CD117, and HLA-DR, and/or are substantially free from such cell surface markers as CD3, CD7, CD 10, CD 13, CD 14, C1319, CD33, CD35, CD56, CD 127, CD 138, and glycophorin A. The population may also comprise cells expressing CD90. The stem cell populations of the invention are isolated from a stem cell source such as bone marrow, peripheral blood, umbilical cord blood, and fetal liver. Methods of the invention comprise isolating and purifying stem cell populations from stem cell sources, and methods of using these cells to reconstitute, repair, and regenerate tissues.

The invention discloses an anti-ageing skincare product. The anti-ageing skincare product comprises 0.001 to 3 weight percent of a plant stem cell active matter and 0.01 to 3 weight percent of Dragosine carnosine, wherein the plant stem cell active matter is at least one of a Phytocelltec<TM> Malus Domestica apple stem cell, a Phytocelltec <TM> Solar Vitis grape stem cell, a Phytocelltec <TM> Alp Rose rose leave stem cell, a Phytocelltec <TM> Argan nut stem cell and a Regenistem Rice Ap red rice stem cell. The preparation method of the anti-ageing skincare product comprises a step of adding the plant stem cell active matter and the Dragosine carnosine into a skincare product. The anti-ageing skincare product comprises the plant stem cell active matter and the Dragosine carnosine at the same time, and under the synergistic action of the two components, the anti-ageing skincare product can obviously improve skin quality, skin elasticity and proliferative capacity of skin, and the effect is obviously higher than that of a skincare product in which the plant stem cell active matter or the Dragosine carnosine is added singly.

The invention discloses a multi-channel nerve repair conduit with a tissue induced function and a mold; chitosan-coated salidroside microspheres and composite type salidroside slow-release microspheres are prepared, the drug cumulative release amount is calculated, the composite type salidroside slow-release microspheres with different contents are mixed with I-type collagen, and salidroside slow-release microspheres/I-type collagen is obtained; a multi-aperture cylindrical nerve conduit core layer is prepared by using the mold, a nano fiber nerve conduit shell layer is prepared by a high-pressure electrostatic spinning technology, the core layer and the shell layer are nested, and thus the multi-channel nerve repair conduit with the tissue induced function is prepared. The shell layer of the conduit has a good role in exchanging with a tissue fluid, and has a function of guiding nerve growth; the core layer has the functions of guiding nerve fiber orientation growth, promoting stem cells to directionally differentiate to schwann cells and accelerating the nerve fiber growth and function recovery, effectively repairs peripheral nerve defects, has good degradation and biocompatibility, and meets the requirements of a tissue engineering scaffold material.

The invention relates to a microfluidic diffusion and open intervening cell culture array chip and a fabrication method and application thereof. The cell culture array chip is suitable for cell culture, stem cell proliferation, drug screening, regenerative medicine and the like. The cell culture array chip comprises a base and a substrate fixed on the base, wherein a plurality of hole-like cell culture unit are distributed on the substrate; and a microfluidic main channel and a microfluidic diffusion channel, which are intercommunicated with each other, are arranged on the substrate and are communicated with each cell culture unit and the external environment. The cell culture array chip provided by the invention has the following advantages: (1) the required culture solution, growth factors and the like are supplied through a microfluidic diffusion network, so as to result in a zero cell shearing force and ensure the gas and moisture environment required for cell growth; (2) the in vivo microenvironment multichannel microfluidic concentration gradient of cells is simulated, and the quantitative drug release at a fixed time and other functions are realized; (3) the chip is suitable for high-throughput drug screening research on cell phenotype; and (4) the chip has a compact structure and low cost, and is suitable for batch production.

A method of ex-vivo expanding a population of stem cells, while at the same time inhibiting differentiation of the stem cells. The method comprises ex-vivo providing the stem cells with conditions for cell proliferation and with at least one copper chelator in an amount and for a time period for permitting the stem cells to proliferate and, at the same time, for reducing a capacity of the stem cells to differentiate

Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.

Disclosed herein are methods for generating SC-β cells, and isolated populations of SC-β cells for use in various applications, such as cell therapy.

The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.

The invention discloses oxidized graphene doped nano-fibers, as well as a preparation method and application thereof. The nano-fibers are continuous nano-fibers with smooth surfaces and mainly formed by oxidized graphene and poly(lactic-co-glycolic acid) using an electrostatic spinning method. The nano-fibers provided by the invention can be used to form a porous three-dimensional structured nano-fiber scaffold material for tissue engineering. The scaffold material for tissue engineering simulates cell natural extracellular matrix, wherein the diameter of the nano-fiber ranges from a dozen nanometers to several micrometers, and the nano-fiber is similar with the collagen fiber in the extracellular matrix; the scaffold material provided by the invention has a porous topology structure with a high specific surface area and simulating the extracellular matrix, can provide better growth environment for the cells, can remarkably promote the adhesion, proliferation and differentiation of the stem cells; at the same time, the preparation technology of the nano-fibers is simple without using complicated equipment; mass production can be achieved, and the manufacturing cost is low.

The invention relates to the technical field of cell culture, and in particular to a serum-free culture medium, a preparation method thereof and a culture method of mesenchymal stem cells. The culturemedium consists of the following components: non-essential amino acid, glutamine, hydrocortisone, dexamethasone, polyvinyl alcohol, recombinant human insulin, recombinant human transferrin, a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor, a recombinant human Wnt-3a protein, a recombinant human fibronectin, recombinant human laminin, L-glutathione, L-ascorbic acid, beta-mercaptoethanol, ethanolamine, Pluronic F-68, Tween 80, cholesterol, adenine, sodium selenite and a basic culture medium. According to the invention, polyvinyl alcohol can replaceserum albumin in a serum-free culture medium in carrier function, so that proliferation capacity of mesenchymal stem cells is effectively improved. Polyvinyl alcohol has more stable performance thanserum albumin, and the problem that proliferation capacity and passage capacity of stem cells are reduced can be effectively solved.

Provided are functional cell and tissue assay systems based on substrate-integrated multifunctional microelectrode arrays implementing stem cell technology. The system covers normal and pathogenic characteristics.

A carrier for growing stem cells is provided, the carrier comprises a substrate comprising one or more outer surfaces; and a hydrophilic, water soluble coating material disposed and dried on one or more of the outer surfaces. The carrier comprises one or more structured indentations on one or more of the outer surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of culturing stem cells and stromal cells using the same carrier are also provided.

The instant invention provides methods and compositions for the production and use of pluripotent stem-like cells from somatic cells, e.g., fibroblasts.

The invention provides tissue culture system for primary cells (e.g. normal mammalian primary epithelial progenitors). This system includes: a) a serum-free, chemically defined cell culture media; and, b) methods for isolation and in vitro long-term propagation of primary cells (e.g. primary epithelial cells). Primary cells so isolated and cultured can be kept undifferentiated and proliferate for many weeks (>15 weeks) or population doubling (>35 PD) without senescence, or any detectable genetic alterations. Upon changing media/culture conditions, these cells can be induced to differentiate.

The invention also provides methods to transform normal primary cells so cultured into “cancer stem cells.” The genetically defined cancer stem cell tumor model mimics the behavior of the disease closely, e.g., the cells are invasive, hormone responsive and metastatic when injected into mice. The tumor cells express genes that are specific to cancer stem cells identified in patient samples.

A stem-cell-seeded porous scaffold implant and electromagnetic growth stimulation for treating or augmenting a breast tissue defect in a patient.

This invention relates to a sensor for detecting a stem cell differentiation, including (a) an electrode; and (b) a substrate of an alkaline phosphatase. The phosphorylation or dephosphorylation of the substrate for an alkaline phosphatase as a stem cell undifferentiation marker which dephosphorylates its substrate may be measured using an electrical signal in the present sensor. Therefore, the sensor of the present invention enables to electrically detect a stem cell status in a high-throughput manner and to determine the stem cell differentiation.

The invention discloses a method for effectively inducing stem cells to differentiate into pancreas islet beta cells. The method comprises the following steps: under the condition of inducing cell agglomeration, firstly treating the cell cluster through adopting an MET agonist, so as to achieve the method for effectively inducing stem cells to differentiate into pancreas islet beta cells. According to the method, the efficiency and vigour of stem cells differentiation into insulin secretory cells are improved, and hyperglycemia caused by the situations that beta cells are damaged and the absolute amount of insulin secretion decreases is treated.

The invention discloses applications of miR-455-3p in the diagnosis, treatment, and prognosis of esophageal squamous cancer. The inventor finds that the obviously low survival rate of ESCC patients is related with the high expression of miR-455-3p in ESCC patients. The in-vitro and in-vivo experiment results show that miR-455-3p is capable of promoting the malignant progression of ESCC, by inhibiting the expression of miR-455-3p, the ESCC stem cell characteristics can be inhibited, and the sensitivity of ESCC stem cells to chemotherapy drugs is enhanced; so an expression inhibitor of miR-455-3p can inhibit the tumor stem cells, and is capable of inhibiting the tumor stem cell characteristics and enhancing the sensitivity of the cancer stem cells to chemotherapy drugs. The invention finds the expression of miR-455-3p in cancer patients for the first time, and the expression can be used as auxiliary diagnosis and/or prognosis of ESCC. The invention provides a novel diagnosis method, a novel treatment method, and a pharmaceutical drug screening platform for tumor diseases.

Compositions for use in treatment of a variety of tissue diseases include stem cells and stem cell released molecules (SRM's) suspended in an aqueous solution with a cellulosic material or other thickening agent. The stem cells and SRM's can be derived from one or more distinct cell lines. The SRM's can further include one or more mucins, cytokines, or growth factors. Exemplary formulations include stem cells and SRMs derived from epithelial stem cells, corneal limbal stem cells, and fibroblasts. Other compositions and methods for formulation thereof are described.

The invention relates to the technical field of biological materials and clinical medicine, and provides an injection, a preparation method of the injection and application of the injection in dentalpulp regeneration. The injection comprises a composite preparation as a dispersion phase and a dispersion liquid as a continuous phase. The composite preparation comprises a drug, a microcarrier and stem cells, the drug is wrapped in the microcarrier, and the stem cells are attached to the surface of the microcarrier. The dispersion liquid is selected from at least one of a group consisting of a balanced salt solution, a serum-free culture medium and injectable hydrogel. The injection is mainly applied to regeneration of clinical functional dental pulp tissues, and regeneration of dental pulpin affected root canals is realized. By utilizing the injection, the formation of the nerve and vascularized dental pulp-like tissue in the root canal can be realized, the nutrition, defense and sensory functions of the dental pulp tissue are recovered, and the service life of the affected tooth is prolonged.

The invention discloses a serum-free culture medium for stem cells. The culture medium contains a basic culture medium component and serum substitutes suitable for the stem cells, wherein the serum substitutes include albumins, growth factors, adherence factors and an albumin substitute, the albumin substitute is capable of at least substituting partial albumins and is polyvinyl alcohol. By specifically utilizing the polyvinyl alcohol, the addition amount of the albumins in the culture medium is reduced; or by substituting the albumins with the polyvinyl alcohol, the cost of the culture mediumis lowered, and separation and purification of products is promoted; and in-vitro culture of the stem cells reaches a proliferation effect better that of a serum culture medium.

The invention provides a proliferation culture medium for 3D simulation culture of stem cells, the proliferation culture medium is free of serum addition, definite in composition and suitable for 3D simulation perfusion culture of stem cells, and comprises inorganic salt, amino acid, saccharides, lipids, nucleosides, vitamins, cell metabolites, cell proliferation factors and water, the osmotic pressure of a culture solution is 270-330mOsmol/kg.H2O, and the pH value of the culture solution is 7.2-7.4.

The invention relates to a proliferation culture medium for 3D simulation culture of stem cells, the proliferation culture medium is free of serum addition, clear in composition and suitable for 3D simulation perfusion culture of stem cells, and comprises inorganic salt, amino acid, saccharides, lipids, nucleosides, vitamins, cell metabolites, cell proliferation factors and water, the osmotic pressure of a culture solution is 270-330mOsmol/kg.H2O, and the pH of the culture solution is 7.2-7.4.

The invention discloses a stem cell heat insulation and moisturizing transport box. The transport box comprises a box body, a box cover arranged at the top of the box body and a handle arranged at thetop of the box cover, and further comprises a stem cell cryopreservation tube fixing device, a temperature control device, an ultrasonic humidifying device, a control panel, a wireless communicationmodule and a controller. A miniature camera device is arranged at the bottom of the box cover, and protective layers wrap the outer side of the box body and the outer side of the box cover; a first horizontal partition plate is arranged in the box body, the box body is divided into two chambers, a stem cell storage chamber is arranged above the first horizontal partition plate, a humidifying chamber is arranged below the first horizontal partition plate, the ultrasonic humidifying device is arranged in the humidifying chamber, and the stem cell cryopreservation tube fixing device and the temperature control device are arranged in the stem cell storage chamber; and the control panel, the wireless communication module and the controller are all arranged at the top of the box cover. The transport box is simple in structure and convenient to use, the heat insulation and moisturizing effects are excellent, transportation of stem cells is facilitated, it is ensured that the state of the cells in the transportation process is good, and users can monitor the state in the box in real time.

The invention discloses a stem cell culture system. The stem cell culture system comprises a mounting box, a pretreatment mechanism, a cell dispersing mechanism, an isolating and cleaning mechanism, aconvenient test tube picking and placing mechanism, a temperature control mechanism and a cell centrifugation mechanism, wherein the bottom end of the mounting box is fixedly connected with a mounting bottom plate, the cell centrifugation mechanism is arranged on one side face of the outside of the mounting box and comprises a centrifuge main body, and a sealing cover is hinged to one side of oneside face, where the centrifuge main body is placed, of the outside of the mounting box. According to the stem cell culture system, a lifting outer tube can be lifted from the top of a lifting circular tube through an offsetting spring, a top end plane of a connecting flip plate can restores horizontal, thus, a fixing ring and a top end plane of a placement tube can be horizontal, then, the placement tube can correspond to a test tube position of the convenient test tube picking and placing mechanism, thus, a test tube can be more easily placed inside the placement tube, and thus, a cell dispersing device and a stem cell centrifugation mechanism can be more conveniently used in a matched manner.

The invention discloses a preparation method and application of cross-linked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca<2+> are crosslinked through a physical crosslinking method to form double-network hydrogel with high mechanical strength, and then the hydrogel is coated with heparin and collagen through a dipping coating method, so that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, the extracted primary muscle stem cells are inoculated onto the hydrogel and cultured for 24 hours in a growth culture medium (79% of DMEM, 10% of FBS and 1% of double antibodies); and then the extracted primary muscle stem cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 days. By utilizing the hydrogel, the absorption of the muscle stem cells on nutrient substances can be enhanced, and the growth of the muscle stem cells is facilitated. The dual-network hydrogel has the potential to be used as a scaffold for muscle stem cell growth of stem cell culture meat.

A method of selecting stem cells from heterogeneous population of cells is disclosed. The method comprises contacting the population of cells with an apoptosis inducing agent under conditions which are apoptotic to non-stem cells and non-apoptotic to stem cells, thereby selecting the stem cells from the heterogeneous population of cells. The selected stem cells may then be used for a variety of applications including transportation and differentiation.

The invention discloses a preparation method of hair follicle stem cells. The method comprises the following steps: cutting roots of hair follicles of volunteers, performing cleaning, adding 0.25% Dispase enzyme, and performing digesting at 37 DEG C for 0.5-1.5 hours; after enzyme digestion, and inoculating the obtained material into a hair follicle stem cell culture medium, wherein the culture conditions are that the temperature is 37 DEG C, the CO2 content is 5 percent, and a liquid is changed once every 1 to 2 days; subjecting hair follicle stem cells to amplification: transferring the hairfollicle stem cells obtained in the step S2 into a hair follicle stem cell amplification culture medium for continuous culture, wherein the hair follicle stem cell amplification culture medium comprises the following components: a DMEM basal culture medium, FBS, T-beta, hEGF, VEGF and Rho related kinase; and subjecting the hair follicle stem cells to purification. The hair follicle stem cells prepared by the method disclosed by the invention can be subjected to long-term in-vitro subculture, have very high regeneration and differentiation capacities, lay a foundation for being applied to treatment of skin defects, and have a very good clinical application prospect.