3769 results about "Stem like cell" patented technology

The invention relates to the technical field of biological information, in particular to a stem cell culture information management method and system. The method comprises the following steps: collecting culture process basic data in a stem cell culture process, wherein the culture process basic data comprises cell counting data, culture condition parameter data and pollution condition data; performing growth kinetic analysis on the basic data in the culture process to obtain cell growth characteristic data; establishing a cell growth prediction model based on the cell growth characteristic data, and generating cell growth early warning data; and monitoring the culture process in real time according to the cell growth early warning data, triggering an early warning signal when abnormal fluctuation is detected, and generating a culture abnormality processing scheme. The growth prediction model is established based on the cell growth characteristic data, the culture dynamic state can be monitored in real time, an early warning signal is provided, the detection and response speed of abnormal fluctuation is remarkably improved, and culture failure is avoided.

The invention discloses an intelligent stem cell extraction monitoring method and system based on the Internet of Things, and relates to the technical field of intelligent medical monitoring. The stem cell extraction intelligent monitoring method and system based on the Internet of Things comprises the steps that S1, multi-source data in the stem cell extraction process is collected and preprocessed; s2, evaluating a modal response slope, sudden change delay and disturbance intensity, and dynamically adjusting a sampling strategy; s3, analyzing modal abnormal fluctuation sensitivity, and driving event anchor point judgment and trend identification; s4, judging a multi-source feature coupling state and a stability level, and triggering a visual mode; and S5, identifying trend abnormity, constructing a dynamic baseline, and executing semantic correction and path reconstruction. The core problems of feature mismatching and decision misleading caused by advanced image variation and chemical response delay in the prior art are solved, and the multi-modal fusion quality and the accuracy and stability of joint anomaly detection are remarkably improved.

The invention discloses a bionic nanofiber-hydrogel composite biological scaffold as well as a preparation method and application thereof. The bionic nanofiber-hydrogel composite biological scaffold is a composite scaffold for simulating a gray-white matter partition structure of a spinal cord tissue, and comprises bionic nanofibers and a bionic hydrogel material, wherein the bionic nanofibers are arranged in an oriented manner and simulate a white matter ordered structure around the spinal cord, and the bionic hydrogel material is combined with the bionic nanofibers and simulates gray matter in the center of the spinal cord. According to the bionic nanofiber-hydrogel composite biological scaffold, the biological activity of loaded stem cells or adult cells can be improved, the problem that a large number of transplanted exogenous cells die due to unbalanced oxidative stress generated after tissue damage can be solved, and it is guaranteed that the transplanted cells reside in a damaged area and play a role; therefore, the spinal cord microenvironment is improved, proliferation and differentiation of endogenous neural stem cells are promoted, functional recovery after spinal cord injury is finally promoted, and great potential is achieved in spinal cord injury treatment.

The invention relates to a stem cell bioactive nano-vesicle with efficient inflammation regulation and tissue repair effects, the average particle size of the nano-vesicle is 100-350nm, the polydispersity index (PDI) is 0.05-0.40, and the expression rate of phosphatidylserine (PS) on the surface of the nano-vesicle is greater than 40%. According to the preparation method, stem cells are subjected to apoptosis induction, and then a programmed serial extrusion process is adopted to prepare the nano-vesicles. The nano vesicles are moderate in particle size, uniform in distribution and high in production efficiency, the preparation process is easy to operate, the quality is stable and controllable, and the nano vesicles have excellent technical effects in the aspects of repair and regeneration of inflammation injury type tissues and organs.

The invention relates to the technical field of image recognition, in particular to a stem cell classification method and system based on image segmentation. The method comprises the following steps: acquiring original stem cell image data, and performing Wasm processing on the original stem cell image data to obtain stem cell image quality parameters; performing adaptive preprocessing on the original stem cell image data based on the stem cell image quality parameters to obtain standardized stem cell image data; and performing multi-resolution pyramid construction on the standardized stem cell image data to obtain multi-level stem cell image representation data. According to the method, through multi-resolution pyramid construction, hierarchical processing of cell regions, accurate morphological feature extraction and hierarchical classification and distributed storage processing, the accuracy, reliability, adaptability and expandability of stem cell image classification are effectively improved.

The invention discloses brain-targeted stem cell membrane bionic cationic liposome as well as a preparation method and application thereof. The preparation method comprises the following steps: extracting and purifying to obtain a stem cell membrane; preparing a cationic liposome; the stem cell membrane and the cationic liposome are fused to obtain the stem cell membrane bionic cationic liposome, and the relative dosage of the stem cell membrane and the cationic liposome is that the mass ratio of the total protein mass of the stem cell membrane to the total fat mass of the cationic liposome is 1: (2-24). The stem cell membrane bionic cationic liposome constructed by using the purified stem cell membrane has high brain targeting ability, low immune irritation and efficient nucleic acid drug carrying ability, can break through the blood brain barrier, realizes efficient targeting delivery of nucleic acid drugs to cells of the brain including neurons, and has good application prospects. Particularly, the polypeptide can be used as a targeted delivery platform of nucleic acid drugs for targeted therapy of cerebral diseases, especially cerebral arterial thrombosis.

The invention belongs to the field of biomedical materials, and particularly discloses a preparation method and application of a 3D printing porous composite scaffold. I-type collagen and silk fibroin composite gel is modified on a 3D printing three-element doped hydroxyapatite / polycaprolactone (PCL) composite scaffold with a polydopamine coating, the three-element doped hydroxyapatite is synthesized by using a chemical precipitation method, and Mg < 2 + >, Sr < 2 + > and SeO3 < 2-> ions are doped in crystal lattices. The 3D printing porous composite scaffold provided by the invention effectively simulates the structure and function of soft and hard combination of natural bones, is helpful for regulating and controlling the stem cell fate to improve the bone regeneration process, repairs bone defects while eliminating tumors, and can be applied to bone defect repair or osteosarcoma treatment.

The invention relates to the field of remote monitoring, in particular to a stem cell culture process monitoring method based on the Internet of Things. Comprising the steps that environmental parameters are collected, sensor data are obtained, the sensor data are verified through a median filtering algorithm, and whether the sensor data are abnormal or not is judged; based on the sensor data marked as normal, calculating by using a nonlinear dynamic coupling analysis algorithm to obtain an environment state index; based on the environment state index, using a non-linear state monitoring prediction algorithm to predict and obtain an environment state index at a future moment; based on the predicted environmental state index of the future moment, using an intelligent early warning trigger algorithm based on environmental state prediction to obtain an early warning state, and generating an early warning signal according to the early warning state; and based on the early warning signal, a dynamic feedback adjustment algorithm is used to automatically generate a control signal. The technical problem that normal operation is interfered due to the fact that a wrong early warning signal is easily triggered by misjudgment when the environment state changes critically is solved.

The invention relates to the technical field of biology, and discloses a stem cell exosome anti-hair loss and hair growth complex and a preparation method thereof.A hair growth agent is wrapped and loaded in adipose-derived mesenchymal stem cell exosome to form a drug-loaded stem cell exosome; the medicine-carrying stem cell exosome is loaded in cross-linked hydrogel composed of a gentisic acid-cyclodextrin complex and epsilon-polylysine modified hyaluronic acid to form the stem cell exosome anti-hair loss and hair growth complex, and the stem cell exosome anti-hair loss and hair growth complex is directly smeared and externally applied to the scalp surface and acts on the hair follicle infection part in a targeted mode, so that the hair loss prevention and hair growth promotion effect is achieved. The stem cell exosome anti-hair-loss and hair-growth-promoting compound can inhibit bacterial growth, relieve and treat hair follicle inflammation caused by bacterial infection and improve the scalp environment, so that under the condition that inflammation is effectively controlled, the drug-loaded stem cell exosome in the stem cell exosome anti-hair-loss and hair-growth-promoting compound is stably released, damaged hair follicle cells are repaired, hair regeneration is promoted, the exosome treatment effect is prolonged, and the anti-hair-loss and hair-growth-promoting effect is achieved. The purpose of treating alopecia is achieved.

The invention belongs to the technical field of biomedical materials, and particularly relates to adipose-derived mesenchymal stem cell loaded conductive hydrogel and a preparation method and application thereof, the preparation method comprises the following steps: step S1, after hyaluronic acid is subjected to oxidation treatment, dopamine is grafted to oxidized hyaluronic acid, and oxidized hyaluronic acid-dopamine is obtained; step S2, carrying out amination treatment on gelatin to obtain aminated gelatin; s3, mixing the oxidized hyaluronic acid-dopamine with the aminated gelatin, and carrying out Schiff base crosslinking, so as to obtain hydrogel; and step S4, loading the adipose tissue-derived stromal cells by using the hydrogel. Dopamine is grafted on an oxidized hyaluronic acid macromolecular chain through amidation reaction, the hydrophilicity and the electrical property of the macromolecular chain are improved, a catechol structure is provided to provide possibility for iron ion coordination, the tissue adhesion, the injectability, the mechanical property and the electrical conductivity of the hydrogel are improved, and the hydrogel can be applied to the field of biomedical materials. After being applied to a spinal cord injury rat model, the functional recovery can be promoted.

The invention relates to the technical field of biological tissue engineering, and discloses a nerve-blood brain barrier co-culture model, which comprises a culture chip, and nerves and blood brain barriers respectively constructed in upper culture micropores and lower culture micropores of the culture chip. Wherein a semi-permeable membrane is arranged between the lower-layer culture micropores and the upper-layer culture micropores of the culture chip; the nerves are obtained by inoculating neurons / brain organs obtained by stem cell differentiation into the upper culture micropores for co-culture, and the blood brain barrier is obtained by inoculating brain microvascular endothelial cells obtained by stem cell differentiation into one side of the semipermeable membrane in the lower culture micropores for co-culture. The co-culture model is higher in biomimetic property, and can better simulate the structure and function of the blood brain barrier, for example, the co-culture model has a barrier function closer to the physiological level, the selective permeability of a small molecule drug is matched with the in-vivo permeation condition, and the co-culture model has the function of a small molecule related transporter. The invention further discloses a construction method and application.

The invention relates to a cell culture microcarrier as well as a preparation method and application thereof. The cell culture microcarrier comprises a polymer porous microsphere with an inside-outside through pore channel structure. The cell culture micro-carrier comprises a biodegradable micro-carrier and also comprises a non-degradable micro-carrier, so that the growth of cells in the carrier is facilitated, and the mass transfer of nutrient substances and metabolites is also facilitated, thereby promoting the growth of the cells, enabling the cultured cells to keep good activity and function, and being high in proliferation efficiency; the method is suitable for three-dimensional culture of somatic cells, stem cells, tumor cells and the like.

The invention provides multifunctional hydrogel, drug-loaded hydrogel as well as a preparation method and application of the multifunctional hydrogel and the drug-loaded hydrogel, and belongs to the technical field of biological medicines. According to the multifunctional hydrogel provided by the invention, the gelatin, the transglutaminase and the sulfhydrylated hyaluronic acid are used as preparation raw materials, so that the problem of limited performance of a hydrogel product in the prior art is solved. According to the hydrogel product prepared by the invention, cell adhesion can be provided through a transglutaminase cross-linked gelatin network, meanwhile, an ROS removal effect is provided through a dynamic disulfide bond cross-linked hyaluronic acid network, and degradation of the hydrogel can be regulated and controlled through combination of the two networks; according to the drug-loaded hydrogel provided by the invention, the exosome derived from neural stem cells is additionally encapsulated in the hydrogel, the exosome and the hydrogel jointly achieve the effects of enhancing inhibition of glial scars and resisting inflammation, and the material provided by the invention can promote repair and regeneration of neurons and myelin sheaths, so that the drug-loaded hydrogel can be used for preparing cerebral hemorrhage treatment drugs and can be used for preparing drugs for treating cerebral hemorrhage. And an effective means is provided for the treatment of related diseases.

The invention provides a whole-process intelligent management system for stem cell culture medium development, and belongs to the technical field of intelligent management. According to the method, artificial intelligence, big data analysis and knowledge graph technologies are fused, and the whole process from demand analysis, formula intelligent design and virtual simulation, small-scale culture verification and iterative optimization, pilot scale-up process optimization to quality compliance and tracing is covered. All the modules are coordinated through the central intelligent decision engine, the research and development efficiency of the culture medium can be remarkably improved, the development cost can be greatly reduced, and batch consistency and quality stability of the culture medium are ensured by reducing trial and error and optimizing in real time. Meanwhile, a full-chain electronic tracing function is provided, the requirements of laws and regulations for compliance are comprehensively met, and the problems that traditional research and development are low in efficiency, high in cost, unstable in batch and the like are effectively solved.

The invention provides application of an enhanced and optimized mesenchymal stem cell exosome in knee osteoarthritis and muscle cartilage injury, and belongs to the technical field of biological medicine. The treatment activity of the umbilical cord mesenchymal stem cell exosome is remarkably improved by combining a composite pretreatment technology with a 3D culture system. Through the synergistic effect of the total flavonoids of rhizoma drynariae and tanshinone IIA, the pathological process of knee osteoarthritis can be regulated and controlled in multiple dimensions, including inflammatory reaction inhibition, cartilage repair promotion and joint function improvement, and the functional limitation of single-component pretreatment is broken through. The invention provides a safe and efficient novel treatment technical strategy for relieving knee osteoarthritis and muscle cartilage injury, and has a wide clinical application prospect.

The invention relates to the technical field of image processing, and discloses a stem cell microscopic image feature extraction method and system. The method comprises the following steps: carrying out segmentation processing on a stem cell delay 3D confocal microscopic image through a chromatin texture sensitive adaptive threshold segmentation algorithm to obtain a nucleoplasm separation mask image; performing feature extraction processing on the marker fluorescence image according to the mask image to obtain a radial distribution feature vector and a pluripotency maintenance index; performing time sequence quantification processing on the state change sequence based on the index to obtain differentiation track feature data; performing spatial feature extraction processing on the subcellular organelle region through a topological graph construction algorithm to obtain a spatial correlation network graph; and performing feature fusion processing through a multi-layer perceptron fusion algorithm to obtain a stem cell image feature classification result. The accuracy of stem cell state recognition and the reliability of differentiation trajectory prediction are improved.

The invention discloses stem cell culture equipment, and relates to the technical field of cell culture equipment, the stem cell culture equipment comprises a culture box, a rotating disc and a culture dish, horizontal plates are arranged in the middle of the culture box at equal intervals, and the middle of each horizontal plate is connected with the rotating disc through a bearing. The stem cell culture equipment is provided with a switching mechanism and an anti-deviation mechanism, a limiting ring and a circular track are separated, a conical block drives a tray to push culture dishes to be rapidly taken and placed, a rotating disc rotating slowly facilitates personnel to take or place the culture dishes one by one at the position close to an opening of a culture box, the limiting ring and the circular track are in matched butt joint, and the operation is convenient. The extrusion part elastically extrudes the culture dish on the tray when reversely moving and overturning, and meanwhile, the traction balance wheel is propped against the upper edge of a sealing end cover of the culture dish, so that the culture equipment can prevent the culture dish which is rotationally placed from shaking and shifting, and can be used for lifting and ejecting all the culture dishes at one time when the culture dish is taken out; and the operation of manually placing the culture dishes can also be reduced.

The invention provides a mesenchymal stem cell preparation for promoting knee joint repair as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: loading transforming growth factor-beta1 and interleukin-6 on chitosan microspheres, then preparing a chitosan-oxidized starch-carboxymethyl-beta-cyclodextrin bracket loaded with sustained release microspheres, loading luteolin and quercetin, carrying out mixed culture with mesenchymal stem cells, adding a traditional Chinese medicine extract, and carrying out freeze-drying to obtain the luteolin-quercetin-modified mesenchymal stem cells. The mesenchymal stem cell preparation for promoting knee joint repair is prepared. The preparation method of the carbon anode plate for fluorine preparation is simple, the generation of polarization of the carbon anode plate is prevented, and the carbon anode plate has the advantages of low porosity, low ash content, high mechanical strength, high volume density, obviously prolonged service life, low resistivity and the like, and has a wide application prospect.

Systems and methods for applying therapeutic ultrasound to the brain to compensate for attenuation and dephasing of ultrasound by each individual's head. The compensation is based on relative ultrasound through-transmit measurements, which are performed using a set of ultrasonic emitters over one side of the head and a set of receivers on the other side. The measurements are performed with the head absent and present. Based on the difference between these measurements, the set of ultrasound waves is adjusted to compensate for attenuations and dephasing caused by the ultrasound wave passing into the head through the scalp and skull. The adjusted set of ultrasound waves provides intended, deterministic ultrasound intensity at the target location. The deterministic delivery enables safe and effective ultrasonic neuromodulation, local drug release from nanoparticle carriers, and microbubble-based disruption of blood-brain barrier for the delivery of drugs, genes, and stem cells across the blood-brain barrier.

The invention discloses functional injection type bionic hydrogel for promoting bone healing and a preparation method thereof, and relates to the technical field of medical instruments. The hydrogel has good functional characteristics, is a biomimetic material prepared on the basis of methacrylated gelatin, and aims to regulate and control the degradation rate, the drug release mechanism and diversified synergistic treatment and promote bone repair and regeneration; according to the hydrogel, matching of the degradation rate and the bone regeneration rate is achieved through reasonable matching, stem cell osteogenic differentiation and angiogenesis are promoted, ultrasonic controlled-release drug release and synergistic controlled-release of active factors are achieved, inflammation is reduced, and the bone repair process is promoted; the calcium phosphate bone cement provides biocompatibility and bone conductivity, and the nano clay releases active ions to improve the microenvironment and promote bone repair; in addition, the magnetic nanoparticles and a static magnetic field have synergistic interaction, the bone healing process is further accelerated, and treatment effect monitoring is enhanced through magnetic resonance imaging.

The invention provides milk exosome composite hydrogel as well as a preparation method and application thereof, and belongs to the technical field of medical materials. The milk exosome composite hydrogel provided by the invention is prepared from milk exosome, hyaluronic acid, epsilon-polylysine, 3-aminophenylboronic acid and tannic acid. The exosome is extracted from milk of a non-stem cell source; the molecular weight of the hyaluronic acid is 200 to 400 KDa. The invention provides a hydrogel carrier capable of loading a large amount of milk exosomes, and the hydrogel carrier can continuously release a large amount of milk exosomes and can give full play to the anti-oxidation and inflammation inhibition effects of the milk exosomes. In the aspect of wound healing, the milk exosome and the hydrogel carrier have a synergistic effect, so that the healing effect can be remarkably improved, and the healing period is shortened.

The invention provides a whole-process targeting polypeptide, a drug compound thereof, a drug delivery system and application of the whole-process targeting polypeptide. The whole-process targeting polypeptide comprises pHA and VAP which are covalently connected through a bridging structure; the VAP is LVAP, a reverse sequence polypeptide of the LVAP, DVAP or SVAP; wherein the amino acid sequence of the LVAP is as shown in SEQ ID NO: 1; the SVAP is a D-configuration polypeptide of the LVAP; the DVAP is a D-configuration polypeptide of the LVAP inverted sequence polypeptide; the bridging structure is amino-monoethylene glycol-carboxylic acid or a derivative thereof. The whole-process targeting polypeptide not only can cross a blood-brain barrier, target tumor neovascularization, cross a blood-tumor barrier, target tumor mimicry vessels, tumor cells and stem cells thereof, but also has better tumor cell affinity, anti-tumor treatment and in-vivo safety effects. Meanwhile, the drug compound or the drug delivery system has a synergistic effect after being combined with a chemotherapeutic drug, and the anti-tumor drug effect can be remarkably improved.

To provide senolytic agents to eliminate senescent cells and methods for treating diseases and disorders associated with senescence.SOLUTION: Disclosed is a method for removing senescent cells from banked stem cells, the method comprising adding to the stem cells at least one senolytic agent. Also provided is a pharmaceutical composition comprising at least one senolytic agent for use in a method for treating a disease, disorder or condition associated with senescence in a subject, where the method comprises removing stem cells from the subject, culturing the cells with the pharmaceutical composition, and administering to the subject the cultured cells.SELECTED DRAWING: None

The invention relates to the technical field of image processing, in particular to an induced pluripotent stem cell density detection method, computer equipment and a medium, and the method comprises the following steps: acquiring an induced pluripotent stem cell image; presetting a local area of each pixel point; obtaining a local brightness performance value according to the gray level distribution of the pixel points in the local area; obtaining local boundary ambiguity according to the gradient distribution characteristics; obtaining local form regularity according to the uniformity characteristic of gray level distribution in a pixel point local area; obtaining a density comprehensive performance value of each pixel point; clustering the pixel points to obtain a preset number of clusters; and respectively enhancing each cluster by adopting histogram equalization to obtain an enhanced induced pluripotent stem cell image, and carrying out density detection on the enhanced induced pluripotent stem cell image. The invention aims to enhance the induced pluripotent stem cell image and improve the accuracy of the cell density detection result.

The present invention belongs to the field of daily chemicals, and discloses a mitochondrial energy improvement composition, application and cosmetic based on plant stem cells. The composition comprises the following components in parts by weight: 0.0005 - 1 part of sunflower bud extract; 0.0005 - 1 part of lemon balm extract; 0.0005 - 1 part of epigallocatechin gallate or epigallocatechin gallate. The composition of the present invention combines sunflower bud extract, lemon balm extract, epigallocatechin gallate or epigallocatechin gallate, and it is found that the combination of the three has obvious ability to improve cell mitochondrial dysfunction, enhance mitochondrial energy and increase the content of type I collagen, and can be used as a potential alternative for anti-wrinkle active ingredients in cosmetics.

The invention relates to a medicine, a medicine composition and application of the medicine and the medicine composition in treatment of liver fibrosis. The medicine and the medicine composition comprise an umbilical cord mesenchymal stem cell membrane; the umbilical cord mesenchymal stem cell membrane is prepared by the following steps: coating the surface of a temperature-sensitive culture dish with a matrix; adding the cell suspension of the umbilical cord mesenchymal stem cells into a temperature-sensitive culture dish for culturing; the temperature is reduced, the umbilical cord mesenchymal stem cells and the extracellular matrix secreted by the umbilical cord mesenchymal stem cells are separated in a lamellar mode, and the umbilical cord mesenchymal stem cell membrane is obtained. The medicine and the medicine composition provided by the invention can effectively relieve liver fibrosis.

A 3D printed bone defect repair scaffold loaded with extracellular matrix of mesenchymal stem cells is provided, its preparation method includes: dissolving gelatin, sodium alginate and 58S bioglass in water to obtain a solution, stirring the solution evenly to obtain 3D printing slurry, and performing 3D printing; chemically cross-linking the semi-finished scaffold with a calcium chloride solution, followed by soaking in a glutaraldehyde solution to chemically cross-link, and cleaning and freeze-drying to obtain the 3D printed scaffold; sterilizing the 3D printed scaffold; seeding rBMSC suspension on the sterilized 3D printed scaffold at a concentration of at least 106 cells / well, and culturing rBMSC by using a low glucose DMEM with 10% FBS for 2 weeks; and taking out the scaffold to perform a decellularization treatment, and freeze-drying the decellularized 3D printed scaffold to obtain the 3D printed bone defect repair scaffold loaded with extracellular matrix of mesenchymal stem cells.

The invention relates to the technical field of stem cell biology, and discloses a stem cell quality evaluation system and method, and the system comprises a data collection module which collects local environment data and image data of stem cells in a preset range; the morphological recognition module is used for obtaining cellular morphological data of all stem cells in a preset range through a cellular morphological recognition model; the graph structure data construction module is used for constructing graph structure data; the score marking module is used for measuring the multiplication capacity, the genetic stability capacity and the surface marker expression capacity of the stem cells after the preset duration, and obtaining the quality score of the stem cells through manual marking; the model training module is used for training a stem cell quality scoring model through an optimization algorithm; according to the method, the cellular morphology data of the stem cells are extracted through the multi-branch network of the cellular morphology recognition model, and the interaction among the stem cells is comprehensively considered through the stem cell quality scoring model, so that the accuracy of stem cell quality evaluation is improved.

The present disclosure provides bispecific stealth lipid nanoparticle (LNP) compositions engineered to target specific tissues or cell-types, e.g., hematopoietic stem cells, to modify the cells with therapeutic nucleic acid encapsulated in the LNP. The present disclosure also provides compositions and methods of making the LNPs and treatment using the same.

The present disclosure relates to a cell culture medium that promotes cell proliferation during serum-free cell culture.