2475 results about "Stem like cell" patented technology

The invention discloses an intelligent stem cell extraction monitoring method and system based on the Internet of Things, and relates to the technical field of intelligent medical monitoring. The stem cell extraction intelligent monitoring method and system based on the Internet of Things comprises the steps that S1, multi-source data in the stem cell extraction process is collected and preprocessed; s2, evaluating a modal response slope, sudden change delay and disturbance intensity, and dynamically adjusting a sampling strategy; s3, analyzing modal abnormal fluctuation sensitivity, and driving event anchor point judgment and trend identification; s4, judging a multi-source feature coupling state and a stability level, and triggering a visual mode; and S5, identifying trend abnormity, constructing a dynamic baseline, and executing semantic correction and path reconstruction. The core problems of feature mismatching and decision misleading caused by advanced image variation and chemical response delay in the prior art are solved, and the multi-modal fusion quality and the accuracy and stability of joint anomaly detection are remarkably improved.

The invention discloses a bionic nanofiber-hydrogel composite biological scaffold as well as a preparation method and application thereof. The bionic nanofiber-hydrogel composite biological scaffold is a composite scaffold for simulating a gray-white matter partition structure of a spinal cord tissue, and comprises bionic nanofibers and a bionic hydrogel material, wherein the bionic nanofibers are arranged in an oriented manner and simulate a white matter ordered structure around the spinal cord, and the bionic hydrogel material is combined with the bionic nanofibers and simulates gray matter in the center of the spinal cord. According to the bionic nanofiber-hydrogel composite biological scaffold, the biological activity of loaded stem cells or adult cells can be improved, the problem that a large number of transplanted exogenous cells die due to unbalanced oxidative stress generated after tissue damage can be solved, and it is guaranteed that the transplanted cells reside in a damaged area and play a role; therefore, the spinal cord microenvironment is improved, proliferation and differentiation of endogenous neural stem cells are promoted, functional recovery after spinal cord injury is finally promoted, and great potential is achieved in spinal cord injury treatment.

The invention relates to the technical field of image recognition, in particular to a stem cell classification method and system based on image segmentation. The method comprises the following steps: acquiring original stem cell image data, and performing Wasm processing on the original stem cell image data to obtain stem cell image quality parameters; performing adaptive preprocessing on the original stem cell image data based on the stem cell image quality parameters to obtain standardized stem cell image data; and performing multi-resolution pyramid construction on the standardized stem cell image data to obtain multi-level stem cell image representation data. According to the method, through multi-resolution pyramid construction, hierarchical processing of cell regions, accurate morphological feature extraction and hierarchical classification and distributed storage processing, the accuracy, reliability, adaptability and expandability of stem cell image classification are effectively improved.

The invention belongs to the field of biomedical materials, and particularly discloses a preparation method and application of a 3D printing porous composite scaffold. I-type collagen and silk fibroin composite gel is modified on a 3D printing three-element doped hydroxyapatite / polycaprolactone (PCL) composite scaffold with a polydopamine coating, the three-element doped hydroxyapatite is synthesized by using a chemical precipitation method, and Mg < 2 + >, Sr < 2 + > and SeO3 < 2-> ions are doped in crystal lattices. The 3D printing porous composite scaffold provided by the invention effectively simulates the structure and function of soft and hard combination of natural bones, is helpful for regulating and controlling the stem cell fate to improve the bone regeneration process, repairs bone defects while eliminating tumors, and can be applied to bone defect repair or osteosarcoma treatment.

The invention belongs to the technical field of biomedical materials, and particularly relates to adipose-derived mesenchymal stem cell loaded conductive hydrogel and a preparation method and application thereof, the preparation method comprises the following steps: step S1, after hyaluronic acid is subjected to oxidation treatment, dopamine is grafted to oxidized hyaluronic acid, and oxidized hyaluronic acid-dopamine is obtained; step S2, carrying out amination treatment on gelatin to obtain aminated gelatin; s3, mixing the oxidized hyaluronic acid-dopamine with the aminated gelatin, and carrying out Schiff base crosslinking, so as to obtain hydrogel; and step S4, loading the adipose tissue-derived stromal cells by using the hydrogel. Dopamine is grafted on an oxidized hyaluronic acid macromolecular chain through amidation reaction, the hydrophilicity and the electrical property of the macromolecular chain are improved, a catechol structure is provided to provide possibility for iron ion coordination, the tissue adhesion, the injectability, the mechanical property and the electrical conductivity of the hydrogel are improved, and the hydrogel can be applied to the field of biomedical materials. After being applied to a spinal cord injury rat model, the functional recovery can be promoted.

The invention provides a whole-process intelligent management system for stem cell culture medium development, and belongs to the technical field of intelligent management. According to the method, artificial intelligence, big data analysis and knowledge graph technologies are fused, and the whole process from demand analysis, formula intelligent design and virtual simulation, small-scale culture verification and iterative optimization, pilot scale-up process optimization to quality compliance and tracing is covered. All the modules are coordinated through the central intelligent decision engine, the research and development efficiency of the culture medium can be remarkably improved, the development cost can be greatly reduced, and batch consistency and quality stability of the culture medium are ensured by reducing trial and error and optimizing in real time. Meanwhile, a full-chain electronic tracing function is provided, the requirements of laws and regulations for compliance are comprehensively met, and the problems that traditional research and development are low in efficiency, high in cost, unstable in batch and the like are effectively solved.

The invention provides application of an enhanced and optimized mesenchymal stem cell exosome in knee osteoarthritis and muscle cartilage injury, and belongs to the technical field of biological medicine. The treatment activity of the umbilical cord mesenchymal stem cell exosome is remarkably improved by combining a composite pretreatment technology with a 3D culture system. Through the synergistic effect of the total flavonoids of rhizoma drynariae and tanshinone IIA, the pathological process of knee osteoarthritis can be regulated and controlled in multiple dimensions, including inflammatory reaction inhibition, cartilage repair promotion and joint function improvement, and the functional limitation of single-component pretreatment is broken through. The invention provides a safe and efficient novel treatment technical strategy for relieving knee osteoarthritis and muscle cartilage injury, and has a wide clinical application prospect.

The invention relates to the technical field of image processing, and discloses a stem cell microscopic image feature extraction method and system. The method comprises the following steps: carrying out segmentation processing on a stem cell delay 3D confocal microscopic image through a chromatin texture sensitive adaptive threshold segmentation algorithm to obtain a nucleoplasm separation mask image; performing feature extraction processing on the marker fluorescence image according to the mask image to obtain a radial distribution feature vector and a pluripotency maintenance index; performing time sequence quantification processing on the state change sequence based on the index to obtain differentiation track feature data; performing spatial feature extraction processing on the subcellular organelle region through a topological graph construction algorithm to obtain a spatial correlation network graph; and performing feature fusion processing through a multi-layer perceptron fusion algorithm to obtain a stem cell image feature classification result. The accuracy of stem cell state recognition and the reliability of differentiation trajectory prediction are improved.

The invention discloses stem cell culture equipment, and relates to the technical field of cell culture equipment, the stem cell culture equipment comprises a culture box, a rotating disc and a culture dish, horizontal plates are arranged in the middle of the culture box at equal intervals, and the middle of each horizontal plate is connected with the rotating disc through a bearing. The stem cell culture equipment is provided with a switching mechanism and an anti-deviation mechanism, a limiting ring and a circular track are separated, a conical block drives a tray to push culture dishes to be rapidly taken and placed, a rotating disc rotating slowly facilitates personnel to take or place the culture dishes one by one at the position close to an opening of a culture box, the limiting ring and the circular track are in matched butt joint, and the operation is convenient. The extrusion part elastically extrudes the culture dish on the tray when reversely moving and overturning, and meanwhile, the traction balance wheel is propped against the upper edge of a sealing end cover of the culture dish, so that the culture equipment can prevent the culture dish which is rotationally placed from shaking and shifting, and can be used for lifting and ejecting all the culture dishes at one time when the culture dish is taken out; and the operation of manually placing the culture dishes can also be reduced.

The invention provides a mesenchymal stem cell preparation for promoting knee joint repair as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: loading transforming growth factor-beta1 and interleukin-6 on chitosan microspheres, then preparing a chitosan-oxidized starch-carboxymethyl-beta-cyclodextrin bracket loaded with sustained release microspheres, loading luteolin and quercetin, carrying out mixed culture with mesenchymal stem cells, adding a traditional Chinese medicine extract, and carrying out freeze-drying to obtain the luteolin-quercetin-modified mesenchymal stem cells. The mesenchymal stem cell preparation for promoting knee joint repair is prepared. The preparation method of the carbon anode plate for fluorine preparation is simple, the generation of polarization of the carbon anode plate is prevented, and the carbon anode plate has the advantages of low porosity, low ash content, high mechanical strength, high volume density, obviously prolonged service life, low resistivity and the like, and has a wide application prospect.

Systems and methods for applying therapeutic ultrasound to the brain to compensate for attenuation and dephasing of ultrasound by each individual's head. The compensation is based on relative ultrasound through-transmit measurements, which are performed using a set of ultrasonic emitters over one side of the head and a set of receivers on the other side. The measurements are performed with the head absent and present. Based on the difference between these measurements, the set of ultrasound waves is adjusted to compensate for attenuations and dephasing caused by the ultrasound wave passing into the head through the scalp and skull. The adjusted set of ultrasound waves provides intended, deterministic ultrasound intensity at the target location. The deterministic delivery enables safe and effective ultrasonic neuromodulation, local drug release from nanoparticle carriers, and microbubble-based disruption of blood-brain barrier for the delivery of drugs, genes, and stem cells across the blood-brain barrier.

The invention provides a whole-process targeting polypeptide, a drug compound thereof, a drug delivery system and application of the whole-process targeting polypeptide. The whole-process targeting polypeptide comprises pHA and VAP which are covalently connected through a bridging structure; the VAP is LVAP, a reverse sequence polypeptide of the LVAP, DVAP or SVAP; wherein the amino acid sequence of the LVAP is as shown in SEQ ID NO: 1; the SVAP is a D-configuration polypeptide of the LVAP; the DVAP is a D-configuration polypeptide of the LVAP inverted sequence polypeptide; the bridging structure is amino-monoethylene glycol-carboxylic acid or a derivative thereof. The whole-process targeting polypeptide not only can cross a blood-brain barrier, target tumor neovascularization, cross a blood-tumor barrier, target tumor mimicry vessels, tumor cells and stem cells thereof, but also has better tumor cell affinity, anti-tumor treatment and in-vivo safety effects. Meanwhile, the drug compound or the drug delivery system has a synergistic effect after being combined with a chemotherapeutic drug, and the anti-tumor drug effect can be remarkably improved.

The present invention belongs to the field of daily chemicals, and discloses a mitochondrial energy improvement composition, application and cosmetic based on plant stem cells. The composition comprises the following components in parts by weight: 0.0005 - 1 part of sunflower bud extract; 0.0005 - 1 part of lemon balm extract; 0.0005 - 1 part of epigallocatechin gallate or epigallocatechin gallate. The composition of the present invention combines sunflower bud extract, lemon balm extract, epigallocatechin gallate or epigallocatechin gallate, and it is found that the combination of the three has obvious ability to improve cell mitochondrial dysfunction, enhance mitochondrial energy and increase the content of type I collagen, and can be used as a potential alternative for anti-wrinkle active ingredients in cosmetics.

The invention relates to a medicine, a medicine composition and application of the medicine and the medicine composition in treatment of liver fibrosis. The medicine and the medicine composition comprise an umbilical cord mesenchymal stem cell membrane; the umbilical cord mesenchymal stem cell membrane is prepared by the following steps: coating the surface of a temperature-sensitive culture dish with a matrix; adding the cell suspension of the umbilical cord mesenchymal stem cells into a temperature-sensitive culture dish for culturing; the temperature is reduced, the umbilical cord mesenchymal stem cells and the extracellular matrix secreted by the umbilical cord mesenchymal stem cells are separated in a lamellar mode, and the umbilical cord mesenchymal stem cell membrane is obtained. The medicine and the medicine composition provided by the invention can effectively relieve liver fibrosis.

The invention relates to the technical field of stem cell biology, and discloses a stem cell quality evaluation system and method, and the system comprises a data collection module which collects local environment data and image data of stem cells in a preset range; the morphological recognition module is used for obtaining cellular morphological data of all stem cells in a preset range through a cellular morphological recognition model; the graph structure data construction module is used for constructing graph structure data; the score marking module is used for measuring the multiplication capacity, the genetic stability capacity and the surface marker expression capacity of the stem cells after the preset duration, and obtaining the quality score of the stem cells through manual marking; the model training module is used for training a stem cell quality scoring model through an optimization algorithm; according to the method, the cellular morphology data of the stem cells are extracted through the multi-branch network of the cellular morphology recognition model, and the interaction among the stem cells is comprehensively considered through the stem cell quality scoring model, so that the accuracy of stem cell quality evaluation is improved.

The present disclosure provides bispecific stealth lipid nanoparticle (LNP) compositions engineered to target specific tissues or cell-types, e.g., hematopoietic stem cells, to modify the cells with therapeutic nucleic acid encapsulated in the LNP. The present disclosure also provides compositions and methods of making the LNPs and treatment using the same.

The invention relates to the technical field of stem cell culture, and one embodiment of the invention provides a stem cell differentiation culture device which comprises a culture box and further comprises an air inlet pipe, a main fan, a fixed box, a conversion cylinder, a filter cylinder, an auxiliary fan, a discharge box and a cleaning mechanism, the culture box is communicated with a temperature adjusting system through the air inlet pipe, the fixed box is communicated with the air inlet pipe, and the air inlet pipe is communicated with the main fan. The conversion cylinder is rotatably arranged in the fixed box, the interior of the conversion cylinder is divided into two conversion cavities, the filter cylinder is fixedly connected to the interior of the conversion cavities and communicates with the air inlet, the auxiliary fan is installed in the filter cylinder, the discharge box communicates with the fixed box through the discharge port, and the discharge box communicates with a discharge pipe. The cleaning mechanism is used for being matched with the auxiliary fan to clean the inner wall of the filter cartridge. By means of the technical scheme, the technical problem that dust accumulates on a filter screen in a ventilation system in the prior art, and regular disassembly and cleaning are needed is solved.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to a skin moisturizing and repairing composition based on dendrobium officinale and fibroin as well as a preparation method and application of the skin moisturizing and repairing composition. Essence extracted from dendrobium officinale tender shoots obtained through a plant stem cell culture technology and stepped molecular weight fibroin prepared through a composite enzymolysis technology serve as raw materials, the safe and efficient composition is developed, and the actual requirements for skin moisturizing and repairing are met.

The invention discloses a stem cell fusion degree detection method and system based on artificial intelligence, and a storage medium. The method comprises the following steps: carrying out image preprocessing on a cell microscope image; automatically calculating an optimal threshold value by using an image threshold value segmentation algorithm to obtain a cytoplasm mask; median filtering is carried out on the original image to reduce noise, then an adaptive threshold segmentation method is adopted, a local threshold is calculated according to local area gray level distribution, and a cell nucleus binary image is generated; performing connected region marking on the cell nucleus binary image, calculating the area attribute of each region, and performing filtering according to a cell nucleus removal ratio parameter to obtain a cell nucleus mask; performing logic OR operation on the cytoplasm mask and the cell nucleus mask to obtain a complete cell segmentation result; and calculating the fusion degree of the stem cells based on the cell segmentation result. Therefore, the problems of accuracy and consistency of judging the fusion degree of the stem cells by observing microscope images with human eyes in the prior art are solved, and accurate detection of the fusion degree of the stem cells is realized.

The invention discloses a stem cell culture image identification and detection method, and relates to the technical field of stem cell image identification, and the method comprises the following steps: carrying out the graying processing of a stem cell image, obtaining a stem cell gray-scale image, carrying out the binarization processing of the stem cell gray-scale image, obtaining a stem cell binarization image, and obtaining a real-time complete contour based on the stem cell binarization image; acquiring a real-time length-width ratio; obtaining a range of a historical length-width ratio based on the first number of historical mesenchymal stem cells; the qualified real-time length-width ratio is obtained based on the range of the real-time length-width ratio and the historical length-width ratio, and the method is used for solving the problems that in an existing mesenchymal stem cell number recognition technology, the effective number of mesenchymal stem cells in the culture process cannot be accurately extracted, and consequently the accuracy and efficiency of recognizing the number of the mesenchymal stem cells are low; under the condition, the culture quality of the mesenchymal stem cells cannot be effectively judged.

The invention relates to the technical field of biological products, and discloses a mesenchymal stem cell hair growth liquid and a preparation method thereof, and the mesenchymal stem cell hair growth liquid comprises the following components: hair follicle mesenchymal stem cells; a hair follicle mesenchymal stem cell exosome; growth factors: a vascular endothelial growth factor (VEGF), a fibroblast growth factor (FGFs), a keratinocyte growth factor (KGF-7) and interleukin-10 (IL-10); vitamins: vitamin E, vitamin B5, vitamin B2, vitamin B6, vitamin C and biotin; the plant extracts comprise a cacumen biotae extract, a polygonum multiflorum extract, a ginseng extract, a peppermint extract, a purslane extract and a rhodiola rosea extract; other active ingredients: tripeptide and digoxin; the auxiliary material is a hyaluronic acid-collagen peptide graft copolymer; and the balance of a phosphate buffer solution.

The invention belongs to the technical field of stem cell biological preparations, and particularly relates to an anti-aging compound preparation based on stem cells and application of the anti-aging compound preparation in delaying skin aging. The composition is composed of an inner water phase, a middle oil phase and an outer water phase, wherein the inner water phase is prepared from the following components: stem cell exosomes, hyaluronic acid, nicotinamide ribose, trehalose, mannitol, glycine and a citric acid buffer solution; the medium oil phase is prepared from the following components: medium chain triglyceride, shea butter, vitamin E succinate, PEG (Polyethylene Glycol) modified ceramide NP and cholesterol succinic acid monoester; the external water phase is prepared from the following components: polydatin, cellulose nanocrystals and an emulsifying agent. On the basis of a W / O / W emulsion system, a multi-layer anti-aging mechanism is constructed by utilizing a three-dimensional delivery framework of an inner water phase, an oil phase and an outer water phase and space-time synergy of active ingredients, the exosome permeation efficiency is improved through microneedle introduction, and the anti-oxidation and barrier repair effects are achieved by using multiple ingredients.

The invention relates to the technical field of biomedicine, in particular to stem cell exosome-loaded bi-crosslinked hydrogel as well as preparation and application thereof, and the bi-crosslinked hydrogel comprises the following components in parts by weight: 50-90 parts of a matrix polymer; 0.1 to 5 parts of an active load; 1 to 10 parts of cyclodextrin; 5 to 20 parts of modified chitosan; 0.01 to 1 part of a photoinitiator; and 0.01 to 0.5 part of growth factors. Through double-network design of physical / chemical primary cross-linking and photo-initiation secondary cross-linking, the bottleneck that the mechanical property and the machinability of the traditional single cross-linking hydrogel are difficult to consider at the same time is overcome; meanwhile, the human umbilical vein endothelial cell exosome is introduced, and efficient loading, uniform dispersion and degradation coupling slow release of the exosome are realized by virtue of beta-cyclodextrin inclusion and a double-crosslinking network. Therefore, the problems of mechanics, degradation, cross-linking speed or tissue adhesion of cross-linked hydrogel in the prior art are solved.

The invention discloses a stem cell preparation for improving function degeneration of prostatitis and a preparation method and application thereof, and relates to the technical field of stem cells, the stem cell preparation is prepared from mesenchymal stem cells UC-MSCs and ADSCs according to the cell concentration ratio of 1: 2, hyaluronic acid hydrogel is used as a delivery carrier, and the stem cell preparation is prepared from the mesenchymal stem cells UC-MSCs and ADSCs according to the cell concentration ratio of 1: 2. And two key auxiliary components of juniperus formosana extract and mulberry extract are introduced. Wherein the optimal combination of the UC-MSCs and the ADSCs significantly enhances the anti-inflammatory ability and the tissue regeneration effect; the hyaluronic acid hydrogel carrier system effectively improves the targeting delivery efficiency of the stem cells and prolongs the action time; the two natural active components of the juniperus formosana extract and the mulberry extract synergistically improve the local microenvironment of prostate and inhibit inflammatory response and fibrosis process. The stem cell preparation developed through a multi-component synergistic effect mechanism has the characteristics of high targeting property, lasting treatment effect, high safety and the like, and a new strategy is provided for clinical treatment of prostatitis.

The invention provides application of enhanced and optimized mesenchymal stem cells and exosomes in anti-aging or senescence delaying, and belongs to the technical field of biological medicine manufacturing. According to the pharmaceutical composition disclosed by the invention, the activity-enhanced mesenchymal stem cells and the exosomes secreted by the activity-enhanced mesenchymal stem cells are applied in a combined manner, so that a remarkable synergistic interaction effect is shown in anti-aging intervention. Experiments prove that the composition can systematically improve oxidative stress injury related to aging, significantly recover antioxidant enzyme activity of serum and brain tissue to be close to a normal level, and effectively reverse cognitive function degeneration, which is represented as behavioral improvement such as shortening of escape latency, improvement of spatial memory ability and the like; the invention is suitable for preparing drugs or functional preparations for delaying body aging or resisting aging, and provides an innovative technical path for developing an efficient and safe anti-aging therapy.

The invention belongs to the technical field of stem cell exosome preparation, and provides a preparation method and therapeutic application of a stem cell exosome loaded with therapeutic molecules, and the preparation method comprises the following steps: S1, selecting and culturing a stem cell line with exosome secretion capability; s2, in the stem cell culture process, adding selected therapeutic molecules, so that the therapeutic molecules can be taken by the stem cells and integrated into exosomes of the stem cells; s3, collecting stem cell culture supernate, and separating and purifying the exosome through a differential centrifugation, ultrafiltration or density gradient centrifugation method; s4, identifying the exosome obtained by separation; a stem cell line with efficient exosome secretion capacity is selected and cultured, and selected therapeutic molecules are added in the culture process, so that efficient loading of the therapeutic molecules is realized; according to the method, the operation steps are simplified, the loading efficiency is improved, it is ensured that therapeutic molecules can be stably integrated into the stem cell exosome, and meanwhile the biological activity and stability of the exosome are kept.

The invention belongs to the field of daily chemicals, and discloses a mitochondrial energy improving composition based on plant stem cells, application and cosmetics, the composition comprises the following components by weight: 0.0005-1 part of sunflower bud extract; 0.0005 to 1 part of a melissa officinalis extract; and 0.0005 to 1 part of epigallocatechin gallo glucoside or epigallocatechin gallate. According to the composition disclosed by the invention, the sunflower bud extract, the melissa officinalis extract, the epigallocatechin gallyl glucoside or the epigallocatechin gallate are combined, and the combination of the sunflower bud extract, the melissa officinalis extract, the epigallocatechin gallyl glucoside and the epigallocatechin gallate has the effects of obviously improving the cell mitochondrial dysfunction, improving the mitochondrial energy and increasing the content of I-type collagen; the composition can be used as a potential optional scheme of anti-wrinkle active ingredients in cosmetics.

The invention relates to the technical field of cell detection, and particularly discloses a physical detection and analysis device based on stem cell characteristics, the physical detection and analysis device comprises a detection mechanism arranged in an analyzer, the detection mechanism comprises mounting plates, the mounting plates are arranged in an annular array, and the ends, close to each other, of the mounting plates are fixedly connected; according to the stem cell detection liquid preparation device, a test tube is installed in an analyzer through the detection mechanism, so that preparation of the stem cell detection liquid can be completed, and compared with a traditional stem cell detection liquid preparation process, the stem cell detection liquid preparation device has the advantages that the detection efficiency is greatly improved; the test tube needs to be opened for multiple times to extract supernate or add a buffer solution and the like, the operation is simple, the detection and analysis efficiency of the stem cells is improved, stem cell suspension and the like cannot be exposed in an external environment, the damage of the stem cells is reduced, the supernate is conveniently and accurately extracted, and the influence on subsequent detection and analysis use is avoided.

The invention relates to an anti-hair loss and hair growth peptide with alternately arranged D / L amino acids as well as a preparation method and application of the anti-hair loss and hair growth peptide, and belongs to the technical field of biological medicines. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1, D-type and L-type amino acids are alternately arranged and designed, the protease degradation resistance is remarkably enhanced, the in-vitro half-life period is larger than or equal to 48 hours, and beta-catenin protein expression in hair follicle stem cells is activated. The preparation method comprises the following steps: taking Rink Amide resin as a carrier, sequentially coupling D / L type amino acid by adopting an Fmoc solid-phase synthesis method, cracking the resin by trifluoroacetic acid, and purifying by high performance liquid chromatography, so that the yield is more than or equal to 80%, the purity is more than or equal to 96%, and the molecular weight is confirmed to be 956.12 Da by mass spectrometry. The polypeptide can be applied to preparation of anti-hair loss and hair growth cosmetics, medicines and medical instruments, and effectively solves the problems of low stability and insufficient targeting of the polypeptide in the prior art. # imgabs0 #

The embodiment of the invention is suitable for the technical field of biomedicine and image processing, and provides a cell counting method and device and computer equipment, and the method comprises the steps: collecting an adherent stem cell image; segmenting the adherent stem cell image by using a detection segmentation model obtained by training to obtain a plurality of stem cell masks; determining the number of stem cells contained in the adherent stem cell image according to the plurality of stem cell masks; wherein the detection segmentation model comprises a backbone network structure, a convolution layer in the backbone network structure is a multi-layer pyramid convolution, and the multi-layer pyramid convolution is used for extracting multi-scale feature information in the adherent stem cell image. By adopting the method, the adherent stem cells can be rapidly and accurately counted.