41 results about "Cell generation" patented technology

Methods for generating human vascular leptomeningeal cells (VLMCs) from human oligodendrocyte progenitor cells (OPCs) are provided using chemically-defined culture media that allow for generation of differentiated cells in a 34-day culture protocol. Methods of generating human VLMCs from human pluripotent stem cells using chemically-defined culture media in a 40 day culture protocol are also provided. Culture media, isolated cell populations, and kits are also provided.

The invention discloses a method for large-scale production of engineered extracellular vesicles based on freeze-drying and hydration, which comprises the following steps: carrying out freeze dehydration treatment on cells, and then inducing the cells to generate target extracellular vesicles through hydration treatment. The method provided by the invention has good cross-cell type compatibility, is adaptive to suspension cells and adherent cells through a mild membrane reconstruction process, and has good biological safety. The invention further discloses the extracellular vesicles produced by the method and application of the extracellular vesicles.

The present disclosure relates to systems, non-transitory computer-readable media, and methods that analyze gene perturbation machine learning embeddings and clinical observation data sets utilizing machine learning, explainability models, and causal discovery models to generate causal predictions between one or more genes and clinical outcomes. Indeed, in one or more implementations, the disclosed systems identify gene perturbation embeddings generated from cells exposed to perturbations. For instance, the disclosed systems select a cluster of genes from a plurality of genes by applying a clustering model to the gene perturbation embeddings. In some instances, the disclosed systems select gene targets from the cluster of genes by using a machine learning classification model trained on a plurality of features of the clinical observation data set. Moreover, in some instances, the disclosed systems generate the causal prediction from the gene targets and the clinical observation data set utilizing a causal discovery model.

The present application relates to the technical field of cell function detection, and particularly relates to a method for evaluating cell function of HL-60 in monocyte activation test. The method comprises the following steps: (1) preparing HL-60 cell suspension; (2) stimulating with pyrogen; (3) quantifying total protein; (4) detecting by Western blotting; and (5) evaluating cell function. The method is based on Western blotting technology, and combines statistical analysis to realize objective and quantitative determination of the core functional index of the ability of synthesizing IL-6 protein under pyrogen stimulation, can accurately identify cells with pyrogen response function defects, provides clear experimental reference for selection of HL-60 cell generations, can pre-screen qualified cells, reduces pyrogen detection deviation caused by unqualified cell function and improper generations from the source, and effectively improves the reliability and repeatability of pyrogen detection results.

Single-cell multi-omics by co-encapsulating a single cell with two beads, the first an RNA barcoding bead having barcoded mRNA capture primer oligonucleotides attached on the bead surface; and the second a DNA barcoding bead having two types of oligonucleotides releasably attached to the surface: (1) barcoded adapter oligonucleotides that are complementary to oligonucleotides bound to the transposase that are eventually incorporated into gDNA fragments and (2) polyadenylated barcoded oligonucleotides containing the same barcode sequence as the adapters. In addition, integrated analysis of RNA and protein, including intracellular protein, from individual cells using similar co-encapsulation of a single cell, an RNA barcoding bead, and with / without a specific or non-specific protein binding bead in a microwell, to avoid protein fixation by first lysing the cell to liberate intracellular contents, and then capturing protein either on a solid surface or in solution with barcoded affinity reagents.

Provided here are methods directed to the T cell receptor cloning of heat shock protein 60 (HSP60), the generation of HSP60-specific regulatory T cells (Tregs), and adoptive cell transfer of HSP60-specific Tregs for the treatment of autoimmune and other disorders. Methods of treatment also include administering a therapeutically effective amount of an inhibitor of expression or activity of nucleus accumbens-associated protein- 1 (NAC1) along with the HSP60-specific Tregs.

The invention belongs to the technical field of biological medicine, and discloses a medicine for in-vivo engineering CAR-T and a preparation method thereof. The drug provided by the invention comprises a first RNA and a second RNA which are used for coding a chimeric antigen receptor in a specific molar ratio, the second RNA is used for coding at least two cytokines of CCL19, IL-17, IL-15, IL-2 or IL-24, by adopting the drug, the gene delivery efficiency and targeting of in-vivo engineered CAR-T can be improved, better T cell transformation and CAR + T cell generation effects are realized, the CAR-T cell depletion is reduced, and the drug has a good application prospect. According to the present invention, the CAR-T gene is constructed, such that the CAR-T gene can survive for a long time in the tumor microenvironment and continuously provide the treatment effect, has the excellent anti-tumor treatment effect, has the excellent application prospect in the in-vivo engineering CAR-T treatment, and has the important significance on the promotion of the efficient, safe and economical development of the CAR-T treatment.

The invention relates to the technical field of biological medicine, and particularly discloses an LNP-mRNA delivery system for generating CAR-T in vivo and application of the LNP-mRNA delivery system. The delivery system comprises lipid nanoparticles and CAR mRNA wrapped by the lipid nanoparticles, the lipid nanoparticles comprise SM102, cholesterol, DSPC and DMG-PEG2000, and the coding target protein of the CAR mRNA is CLDN18.2. The delivery system is applied to preparation of drugs for treating gastric cancer tumors. The obtained mRNA sequence can translate and express CAR protein targeting CLDN18.2, T cells can be encoded in situ in vivo through an LNP delivery system to generate CAR-T, the T cells are transformed into CAR-T cells in vivo, and a novel, convenient and universal implementation scheme is provided for CAR-T therapy.

The invention relates to the technical field of dendritic cell in-vitro induction methods, and provides a dendritic cell body and an in-vitro induction method and application thereof. Thymic stromal lymphopoietin; the invention relates to a recombinant human FLT3L-Fc fusion protein. And maturing stimulation combination. On the premise of avoiding complex use of equipment, the bottlenecks of DC yield, subgroup purity and functionality are broken through.

The current disclosure provides methods and compositions that provide for the insertion of transgenes in stem or progenitor cells without the deleterious effects to T cell differentiation by in vitro T cell differentiation methods. To accomplish this, the inventors have discovered that expression of the transgenes under the control of promoter regions, such as CD8a or CD8b, allows for the coordinated expression pattern that provides for both: 1) high expression of the transgene in mature T cells and 2) a coordinated level of expression of the transgene throughout the in vitro differentiation method that allows for the production of a population of mature T cells.

This invention relates to Natural Killer (NK) cell populations, to methods of producing the same and therapeutic applications thereof. More specifically, the invention relates to the expansion of NK cells by increasing the expression of specific transcription factors associated with NK cell production.

The present invention relates generally to the field of CAR-T cells. Specifically, the present invention relates to improved methods for obtaining CAR-T cells, and improved CAR-T cells and uses thereof. The method further relates to activating or expressing phosphodiesterase (PDE) in T cells during CAR-T cell generation.

A method of selecting candidate T cell receptors (TCRs) or T cells expressing candidate TCRs with defined characteristics, comprising: a) generating single cell data from a plurality of T cells from a

The invention discloses a human immortalized fallopian tube fibroblast strain as well as a construction method and application thereof, and belongs to the technical field of cell immortalization construction. The preparation method comprises the following steps: firstly, separating, culturing and purifying fibroblasts from human fallopian tube tissues by virtue of an enzyme digestion method, identifying markers of the fibroblasts, then introducing a simian virus 40 large T antigen gene into primary fibroblasts by virtue of a lentiviral vector, and screening by virtue of puromycin to obtain a cell strain capable of stably expressing SV40T. Finally, the human immortalized fallopian tube fibroblast which can be stably passaged is constructed. The cell strain has the characteristics of strong multiplication capacity and high hereditary stability, and the cells are not obviously aged along with the increase of the number of generations of the cells, which indicates that the human immortalized fallopian tube fibroblast line is successfully established. According to the invention, the problem that the in-vitro culture life of primary fibroblasts is limited is solved, and a reliable in-vitro cell model is provided for research in the fields of fallopian tube related diseases, ovarian cancer origin, tumor microenvironment and the like.

The invention belongs to the technical field of biology, and particularly relates to a compound composition for inducing cell migration by controlling the curvature of a membrane and application. The preparation method comprises the following steps: by taking hemagglutinin protein as a membrane protein aggregation unit, connecting sialic acid lactose and rhodamine B in an acylhydrazone bond manner to form a binding and driving unit (R-SL small molecule induction ligand), so as to form self-assembled molecular tweezers (ATS); the preparation process is simple, and the equipment and labor cost is reduced. The constructed self-assembled molecular tweezers realize the polymerization / depolymerization process of membrane protein through a simple chemical regulation method, so that the membrane curvature is efficiently regulated; cell migration is obviously induced, and wound healing is promoted. The cell level test verifies that when the self-assembly molecular tweezers are used for adjusting the curvature of cells, the self-assembly molecular tweezers can promote the cells to generate pseudopods and induce the neogenesis of blood capillaries, and the self-assembly molecular tweezers have universality, induce cell migration and promote the healing process of wounds. The self-assembled molecular tweezers hydrogel does not pull a wound during replacement, so that the compliance of a patient is improved.

A pipe sheet (1) has: a sheet body (10); a first pipe (21) that is provided inside the sheet body (10) and that is for performing a first step in a cell culturing method; a second pipe (22) that is provided inside the sheet body (10) and is for performing a second step in the cell culturing method; first joints (30) that are provided to one opening end of the first pipe (21) and one opening end of the second pipe (22) and are detachably linked to another member; and second joints (40) that are provided to the other opening end of the first pipe (21) and the other opening end of the second pipe (22), and are detachably linked to another member. A first joint (30) of the first pipe (21) and a first joint (30) of the second pipe (22) are positioned at a first end (10a) of the sheet body (10). A second joint (40) of the first pipe (21) and a second joint (40) of the second pipe (22) are positioned at a second end (10b) of the sheet body (10).

This invention discloses a bismuth-copper bimetallic coordination nanomaterial, its preparation method, and its applications, belonging to the technical field of biofunctional materials. The prepared bismuth-copper bimetallic coordination nanomaterial can efficiently generate reactive oxygen species (ROS) under ultrasonic stimulation, initiating the sonodynamic therapy (SDT) process; the Cu in this material... 2+ The weakly acidic environment of the tumor microenvironment can also trigger a Fenton-like reaction, generating cytotoxic hydroxyl radicals (•OH) and oxygen in situ, thus initiating the chemokinetic therapy (CDT) process. Through the synergistic effect of sonodynamic therapy (SDT) and chemokinetic therapy (CDT), they jointly inhibit cancer cell proliferation and promote T cell generation.

To provide a process for generating compositions of engineered cells.SOLUTION: A process comprises: (a) incubating, under stimulating conditions, an input composition comprising T cells enriched for CD4+ primary human T cells, the stimulating conditions comprising the presence of (i) a stimulatory reagent capable of activating one or more intracellular signaling domains of one or more components of a TCR complex and / or one or more intracellular signaling domains of one or more costimulatory molecules and (ii) one or more cytokines, wherein at least one cytokine is or comprises recombinant human IL-2, thereby generating a stimulated composition; and (b) introducing a recombinant receptor into the stimulated composition, thereby generating an engineered composition comprising engineered T cells.SELECTED DRAWING: Figure 1

This article describes a method for generating mature somatostatin-interneurons from human forebrain neural progenitor cells in a two-stage culture protocol using a chemically defined culture medium. Mature somatostatin-interneurons are generated from medial ganglion ridge neural progenitor cells (MGE-NPC), which themselves are derived from pluripotent stem cells. The article also provides the culture medium, isolated cell populations, and kits.

To increase the establishment efficiency of iPS cells in an RNA reprogramming method.SOLUTION: A method for producing iPS cells, comprising culturing, in a ROCK inhibitor-containing medium, blood cells into which mRNA of a reprogramming factor and mRNA encoding p53DD have been introduced.SELECTED DRAWING: Figure 1

In some aspects, methods are provided for generating primordial follicles from human and other mammalian germ cells with high efficiency, high cell quality, and high reproducibility. In some aspects, methods and culture systems are provided for generating an ovarian organoid, such as a cell aggregate consisting of both somatic and germ cells from a source derived from primary and / or stem cells, the ovarian organoid is capable of progressing primordial germ cells into oocytes that initiate meiosis and are capable of follicular assembly. In some aspects, provided embodiments include the step of culturing an ovarian organoid in a fully defined serum-free medium and under conditions that generally employ a low defined serum protein component.

A culture vessel is arranged in a cell culture device and holds: a second cell generated from a first cell; and a culture medium, and the culture vessel includes: a supply port through which a liquid and the culture medium are supplied to the culture vessel, the liquid including the first cell and being from a cell vessel, the culture medium being from a culture medium vessel; and a discharge port through which vessel liquid present in the culture vessel is discharged, wherein the supply port and the discharge port are provided separately from each other.

This invention provides a method for preparing a composition of manipulated cells. [Solution] The method includes (a) a step of incubating an input composition containing CD4+ primary human T cells enriched T cells under stimulating conditions including (i) a stimulating reagent capable of activating one or more intracellular signaling domains of one or more components of a TCR complex and / or one or more intracellular signaling domains of one or more co-stimulatory molecules, and (ii) the presence of one or more cytokines, at least one of which is recombinant human IL-2 or contains recombinant human IL-2, thereby generating a stimulated composition; and (b) a step of introducing a recombinant receptor into the stimulated composition, thereby generating an engineered composition containing engineered T cells.

The invention relates to the technical field of power grid dispatching control, and discloses an anti-thunderstorm industrial power grid dispatching control system and method, and the method comprises the steps: obtaining and fusing power grid real-time operation data, power grid topological structure data and real-time atmospheric field data in real time, and generating a dynamic vulnerability map through a power grid analysis model; identifying a weak point area facing a lightning stroke risk in the power grid and a risk level thereof; dynamically dividing the power grid into a plurality of defense cells based on the high-risk weak spot area; generating a defense plan package for each defense cell, and issuing the defense plan package to an edge controller; and the edge controller enters an autonomous mode after authorization, and executes fault positioning, isolation and power supply recovery control. The system comprises a central processing unit, an edge controller and a sensing and executing network. According to the invention, through dynamic partitioning and edge autonomy, the anti-disaster capability and power supply reliability of the power grid in thunderstorm weather are improved.

The present disclosure relates to systems, non-transitory computer-readable media, and methods that analyze gene perturbation machine learning embeddings and clinical observation data sets utilizing machine learning, explainability models, and causal discovery models to generate causal predictions between one or more genes and clinical outcomes. Indeed, in one or more implementations, the disclosed systems identify gene perturbation embeddings generated from cells exposed to perturbations. For instance, the disclosed systems select a cluster of genes from a plurality of genes by applying a clustering model to the gene perturbation embeddings. In some instances, the disclosed systems select gene targets from the cluster of genes by using a machine learning classification model trained on a plurality of features of the clinical observation data set. Moreover, in some instances, the disclosed systems generate the causal prediction from the gene targets and the clinical observation data set utilizing a causal discovery model.

The application discloses a lactobacillus mucosae and application thereof, the lactobacillus mucosae is preserved in China typical culture preservation center, and the preservation number is CCTCC NO: M 2022551, and the preservation date is May 3, 2022.The application provides a lactobacillus mucosae XWL, and the lactobacillus mucosae XWL is improved by SLS stress, and it is found that the lactobacillus mucosae XWL and the lactobacillus mucosae XWL improved by SLS stress have the advantages of safety, reliability, good effect and the like, can be used for skin moisturizing, free radical scavenging, reducing cell ROS generation and barrier repair, and can also repair SLS damaged cells directionally, so that it has more pertinence and practical significance in cosmetics.

The application discloses application of SPOP gene or protein in regulating fat generation and heat production capacity of animals. The application discloses for the first time that SPOP plays a key role in fat cell generation and heat generation, especially in regulating gene expression related to fatty acid metabolism and energy consumption, which provides an important theoretical basis for developing a new method for regulating fat metabolism, and provides a new potential target for treatment of obesity and metabolic diseases.

A cell culture method includes: extracting, in a culture vessel holding a liquid that includes a first cell to which a magnetic bead that is an example of a magnetic particle is attached, the first cell by discharging the liquid from the culture vessel while the first cell is attracted to the culture vessel by magnetic force; and culturing, in the culture vessel, a second cell by supplying a culture medium to the culture vessel, the second cell having been generated in the culture vessel from the first cell extracted.

The present invention relates to the technical field of cell data analysis and discloses a joint analysis method of single-cell transcriptome data and text descriptions based on a multimodal language model. The method comprises the following steps: obtaining a single-cell RNA sequencing expression matrix and a corresponding cell text description; preprocessing the single-cell RNA sequencing expression matrix and the corresponding cell text description; constructing a multimodal dataset; and achieving a deep fusion of gene expression data and text knowledge by constructing a dual model and a cross-modal projection module, thereby avoiding the limitations of a single modality. During preprocessing, gene expression values ​​and index sequences are retained, the coarse coding method is changed, and the accuracy of cell type recognition is improved. Based on a pre-training strategy of contrastive learning, matching learning, and a cross-modal projection module, fine-grained cross-modal information interaction and sharing is achieved, and the effects of cross-modal tasks such as text generation into cells or cell generation into text are optimized.