19 results about "Cell generation" patented technology

The present application relates to the technical field of cell function detection, and particularly relates to a method for evaluating cell function of HL-60 in monocyte activation test. The method comprises the following steps: (1) preparing HL-60 cell suspension; (2) stimulating with pyrogen; (3) quantifying total protein; (4) detecting by Western blotting; and (5) evaluating cell function. The method is based on Western blotting technology, and combines statistical analysis to realize objective and quantitative determination of the core functional index of the ability of synthesizing IL-6 protein under pyrogen stimulation, can accurately identify cells with pyrogen response function defects, provides clear experimental reference for selection of HL-60 cell generations, can pre-screen qualified cells, reduces pyrogen detection deviation caused by unqualified cell function and improper generations from the source, and effectively improves the reliability and repeatability of pyrogen detection results.

Single-cell multi-omics by co-encapsulating a single cell with two beads, the first an RNA barcoding bead having barcoded mRNA capture primer oligonucleotides attached on the bead surface; and the second a DNA barcoding bead having two types of oligonucleotides releasably attached to the surface: (1) barcoded adapter oligonucleotides that are complementary to oligonucleotides bound to the transposase that are eventually incorporated into gDNA fragments and (2) polyadenylated barcoded oligonucleotides containing the same barcode sequence as the adapters. In addition, integrated analysis of RNA and protein, including intracellular protein, from individual cells using similar co-encapsulation of a single cell, an RNA barcoding bead, and with / without a specific or non-specific protein binding bead in a microwell, to avoid protein fixation by first lysing the cell to liberate intracellular contents, and then capturing protein either on a solid surface or in solution with barcoded affinity reagents.

The invention belongs to the technical field of biological medicine, and discloses a medicine for in-vivo engineering CAR-T and a preparation method thereof. The drug provided by the invention comprises a first RNA and a second RNA which are used for coding a chimeric antigen receptor in a specific molar ratio, the second RNA is used for coding at least two cytokines of CCL19, IL-17, IL-15, IL-2 or IL-24, by adopting the drug, the gene delivery efficiency and targeting of in-vivo engineered CAR-T can be improved, better T cell transformation and CAR + T cell generation effects are realized, the CAR-T cell depletion is reduced, and the drug has a good application prospect. According to the present invention, the CAR-T gene is constructed, such that the CAR-T gene can survive for a long time in the tumor microenvironment and continuously provide the treatment effect, has the excellent anti-tumor treatment effect, has the excellent application prospect in the in-vivo engineering CAR-T treatment, and has the important significance on the promotion of the efficient, safe and economical development of the CAR-T treatment.

The current disclosure provides methods and compositions that provide for the insertion of transgenes in stem or progenitor cells without the deleterious effects to T cell differentiation by in vitro T cell differentiation methods. To accomplish this, the inventors have discovered that expression of the transgenes under the control of promoter regions, such as CD8a or CD8b, allows for the coordinated expression pattern that provides for both: 1) high expression of the transgene in mature T cells and 2) a coordinated level of expression of the transgene throughout the in vitro differentiation method that allows for the production of a population of mature T cells.

The invention belongs to the technical field of biology, and particularly relates to a compound composition for inducing cell migration by controlling the curvature of a membrane and application. The preparation method comprises the following steps: by taking hemagglutinin protein as a membrane protein aggregation unit, connecting sialic acid lactose and rhodamine B in an acylhydrazone bond manner to form a binding and driving unit (R-SL small molecule induction ligand), so as to form self-assembled molecular tweezers (ATS); the preparation process is simple, and the equipment and labor cost is reduced. The constructed self-assembled molecular tweezers realize the polymerization / depolymerization process of membrane protein through a simple chemical regulation method, so that the membrane curvature is efficiently regulated; cell migration is obviously induced, and wound healing is promoted. The cell level test verifies that when the self-assembly molecular tweezers are used for adjusting the curvature of cells, the self-assembly molecular tweezers can promote the cells to generate pseudopods and induce the neogenesis of blood capillaries, and the self-assembly molecular tweezers have universality, induce cell migration and promote the healing process of wounds. The self-assembled molecular tweezers hydrogel does not pull a wound during replacement, so that the compliance of a patient is improved.

This invention discloses a bismuth-copper bimetallic coordination nanomaterial, its preparation method, and its applications, belonging to the technical field of biofunctional materials. The prepared bismuth-copper bimetallic coordination nanomaterial can efficiently generate reactive oxygen species (ROS) under ultrasonic stimulation, initiating the sonodynamic therapy (SDT) process; the Cu in this material... 2+ The weakly acidic environment of the tumor microenvironment can also trigger a Fenton-like reaction, generating cytotoxic hydroxyl radicals (•OH) and oxygen in situ, thus initiating the chemokinetic therapy (CDT) process. Through the synergistic effect of sonodynamic therapy (SDT) and chemokinetic therapy (CDT), they jointly inhibit cancer cell proliferation and promote T cell generation.

To provide a process for generating compositions of engineered cells.SOLUTION: A process comprises: (a) incubating, under stimulating conditions, an input composition comprising T cells enriched for CD4+ primary human T cells, the stimulating conditions comprising the presence of (i) a stimulatory reagent capable of activating one or more intracellular signaling domains of one or more components of a TCR complex and / or one or more intracellular signaling domains of one or more costimulatory molecules and (ii) one or more cytokines, wherein at least one cytokine is or comprises recombinant human IL-2, thereby generating a stimulated composition; and (b) introducing a recombinant receptor into the stimulated composition, thereby generating an engineered composition comprising engineered T cells.SELECTED DRAWING: Figure 1

This article describes a method for generating mature somatostatin-interneurons from human forebrain neural progenitor cells in a two-stage culture protocol using a chemically defined culture medium. Mature somatostatin-interneurons are generated from medial ganglion ridge neural progenitor cells (MGE-NPC), which themselves are derived from pluripotent stem cells. The article also provides the culture medium, isolated cell populations, and kits.

To increase the establishment efficiency of iPS cells in an RNA reprogramming method.SOLUTION: A method for producing iPS cells, comprising culturing, in a ROCK inhibitor-containing medium, blood cells into which mRNA of a reprogramming factor and mRNA encoding p53DD have been introduced.SELECTED DRAWING: Figure 1

This invention provides a method for preparing a composition of manipulated cells. [Solution] The method includes (a) a step of incubating an input composition containing CD4+ primary human T cells enriched T cells under stimulating conditions including (i) a stimulating reagent capable of activating one or more intracellular signaling domains of one or more components of a TCR complex and / or one or more intracellular signaling domains of one or more co-stimulatory molecules, and (ii) the presence of one or more cytokines, at least one of which is recombinant human IL-2 or contains recombinant human IL-2, thereby generating a stimulated composition; and (b) a step of introducing a recombinant receptor into the stimulated composition, thereby generating an engineered composition containing engineered T cells.

The present disclosure relates to systems, non-transitory computer-readable media, and methods that analyze gene perturbation machine learning embeddings and clinical observation data sets utilizing machine learning, explainability models, and causal discovery models to generate causal predictions between one or more genes and clinical outcomes. Indeed, in one or more implementations, the disclosed systems identify gene perturbation embeddings generated from cells exposed to perturbations. For instance, the disclosed systems select a cluster of genes from a plurality of genes by applying a clustering model to the gene perturbation embeddings. In some instances, the disclosed systems select gene targets from the cluster of genes by using a machine learning classification model trained on a plurality of features of the clinical observation data set. Moreover, in some instances, the disclosed systems generate the causal prediction from the gene targets and the clinical observation data set utilizing a causal discovery model.

The application discloses application of SPOP gene or protein in regulating fat generation and heat production capacity of animals. The application discloses for the first time that SPOP plays a key role in fat cell generation and heat generation, especially in regulating gene expression related to fatty acid metabolism and energy consumption, which provides an important theoretical basis for developing a new method for regulating fat metabolism, and provides a new potential target for treatment of obesity and metabolic diseases.

A method of generating adult stem cells from somatic cells is provided. The method involves isolating somatic cells from a donor tissue and culturing the isolated cells in a tissue-specific growth medium. The cultured cells are subjected to proteolytic detachments once they reach a sufficient level of confluency. The confluent somatic cells are treated a culture of somatic cells isolated from a donor tissue with a combination of growth factors and / or chemical compounds. The cells are then reprogrammed by subjecting the cells to a suspension culture treatment without any substrate or beads to attach and are forced to rely on cell-to-cell contact. This treatment leads to formation of aggregates comprising cells that display stem-cell like characteristics.

Methods and systems for generating virtually stained fluorescence images from unlabeled cells. Methods include receiving one or more cell images acquired using an image-based flow cytometer, receiving user input of a species and cell type of the cells in the images, receiving user selection of one or more biomarkers, and generating virtual stained fluorescence images of the one or more cells representative of digital expression data for the one or more biomarkers. Systems include (i) a training device that uses machine learning models to generate an image conversion algorithm based on a training data set having a plurality fluorescence images of individual cells stained with a fluorescent marker, and (ii) a virtual staining device that receives fluorescence images from unlabeled test cells and applies the image conversion algorithm to the fluorescence images to produce a virtually stained fluorescence image.

Provided herein are genetically modified T cells that exhibit increased proliferation upon stimulation compared to wild-type T cells, methods of generating such T cells, and methods of using the T cells to treat diseases such as cancer.

This invention relates to the field of vascular structure recognition technology, and discloses an automated method and system for recognizing vascular structures in OCT images. The method includes: Step 1, vascular layer boundary feature extraction: extracting vascular layer boundary features from OCT images based on multi-scale Hessian matrix response, including geometric and physical features, to form a feature response map; Step 2, three-layer topological structure recognition: constructing the topological relationship of the vascular structure based on the feature response map, extracting nested ring structures; identifying and outputting the initial boundary coordinate point set and structural score of the intima, media, and adventitia; Step 3, vascular structure optimization: precisely optimizing the initial boundary coordinate point set, detecting special cells within the optimized region, and generating a result report. This invention achieves fully automated recognition of OCT vascular structures, ensuring the reliability, accuracy, and interpretability of the recognition results, and ensuring the standardization and unification of vascular lesion diagnosis.

Provided herein are genetically modified T cells that exhibit increased proliferation upon stimulation compared to wild-type T cells, methods of generating such T cells, and methods of using the T cells to treat diseases such as cancer.