86 results about "Cell generation" patented technology

A computer implemented system and method is provided for generating a layout of the cell defining a circuit component, the layout providing a layout pattern for a target process technology. The method comprises obtaining an archetype layout providing a valid layout pattern for the cell having regard to design rules of the target process technology, and receiving an input data file providing a process technology independent schematic of the circuit component for which the cell is to be generated. A schematic sizing operation is then performed on the input data file, having regard to both schematic constraints applicable to the target process technology and layout constraints derived from the archetype layout, in order to generate an output data file providing a process technology dependent schematic of the circuit component. A cell generation operation is then performed using the output data file and layout data determined from the archetype layout in order to generate the layout of the cell. Such an approach enables both the schematic and layout to be co-optimised during generation of the layout of the cell.

The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.

A method of constructing a data architecture for use in identifying connections between perturbagens and genes associated with skin tone. The method includes providing a gene expression profile for a control cell, generating a gene expression profile for a cell exposed to a perturbagen, identifying genes differentially expressed in response to the perturbagen, creating an ordered list of identifiers, storing the ordered list as an instance on a computer readable medium, and constructing a data architecture of stored instances.

An ATM (asynchronous transfer mode) cell transfer apparatus includes an input interface, a switch block, and an OAM cell processing hardware block having a memory unit. The input interface receives an SDH/SONET signal on each of a plurality of first transfer paths to output an input OAM cell corresponding to the SDH/SONET signal to one of a plurality of input ports of the switch block corresponding to the first transfer path for the SDH/SONET signal to be transferred. The switch block receives the input OAM (operation and maintenance) cell from the corresponding input port as an OAM input port to output to the OAM cell processing hardware block together with a port number of the OAM input port, and receives at least one output OAM cell from the OAM cell processing hardware block to output to at least one of the plurality of output ports based on the received output OAM cell. The OAM cell processing hardware block reads out the at least one output OAM cell corresponding to the input OAM cell from the memory unit based on the input OAM cell and the port number supplied from the switch block, and outputs the at least one output OAM cell to the switch block.

A communication apparatus is provided which comprises: extraction means for extracting a virtual path identifier and a virtual channel identifier stored in a first header of first cells output from a terminal; preparation means which, based on the extracted virtual path identifier and virtual channel identifier, refers to a conversion table to prepare a second header containing a corresponding identifier; cell generation means for generating second cells containing the prepared second header and the user information of the first cells; multiplexing/generation means for multiplexing the generated second cells so as to have a fixed length, thereby generating third cells; and send means for sending the generated third cells to other communication apparatus through a virtual channel. By virtue of this construction, the usage charge of communication lines, such as ATM lines, can be reduced.

The invention belongs to the field of gene therapy and relates to preparation and an application of an expression vector for targeted inhibition of HIV-1 provirus expression by use of embedded DNA methyltransferase adopting a zinc finger structure. The expression vector carrying the DNA methyltransferase adopting the zinc finger structure is proved to be able to inhibit silence of HIV-1 provirus expression in HIV-1 infection and latent infection cell models, the vector can effectively inhibit virus replication in virus infected cells and latent infected cells for 15-40 days or above. Besides,provirus expression silence caused by the expression vector carrying the embedded methyltransferase adopting the zinc finger structure can be inhered among cell generations and has certain safety in cells. A new idea is provided for curing AIDS.

The invention belongs to the field of solar energy utilization and relates to a solar full-sealing aquaculture constant-temperature workshop which comprises an aquaculture system (01), a water body allocating system (02), a pipeline system (03), a solar energy system (04) and a monitoring system (05). The aquaculture system (01) adopts a light-steel thermal insulation wall body and stair-shaped laminated aquaculture groove structure; a backwater purifying tank (69) and a disinfecting thermoregulation tank (75) of the water body allocating system (02) are arranged under a grade level (80) and penetrate through the bottom of the whole aquaculture unit; the solar energy system (04) obtains electric energy and thermal energy with high changing efficiency by means of the lens group converging, photovoltaic cell generation, water cooling radiation and sunshine automatic tracking technology; an electric pipeline (58), an air supply pipe (71) and a water feeding pipe (72) of the pipeline system (03) are communicated with all functional parts; and the monitoring system (05) controls stable operation of the solar full-sealing aquaculture workshop in program mode through a programmable logic control (PLC) automatic element.

The invention provides an ST2-binding antibody or a fragment thereof. The antibody or a fragment thereof can specifically bind to human ST2, inhibit binding of IL-33 and the human ST2, block an IL-33/ST2 intracellular signal pathway, and inhibit a promotion effect of different forms of IL-33 on generation of cells IL5, IL6 and IL8, and has higher biological activity compared with a known anti-ST2 antibody. The antibody can be used for preventing, treating or relieving diseases related to ST2 expression or IL-33/ST2 pathway disorder.

A computer implemented system and method is provided for generating a layout of the cell defining a circuit component, the layout providing a layout pattern for a target process technology. The method comprises obtaining an archetype layout providing a valid layout pattern for the cell having regard to design rules of the target process technology, and receiving an input data file providing a process technology independent schematic of the circuit component for which the cell is to be generated. A schematic sizing operation is then performed on the input data file, having regard to both schematic constraints applicable to the target process technology and layout constraints derived from the archetype layout, in order to generate an output data file providing a process technology dependent schematic of the circuit component. A cell generation operation is then performed using the output data file and layout data determined from the archetype layout in order to generate the layout of the cell. Such an approach enables both the schematic and layout to be co-optimised during generation of the layout of the cell.

Computerized image guided control method uses image quantification to dynamically monitor and control the cell generation process. The method acquires images during the cell generation steps and uses computer image analysis to automatically quantify the results of the cell generation steps. In one embodiment, the quantified results from the image analysis are used to determine the readiness of the cell generation step. In another embodiment, a remedial recovery sub-step for the cell generation step is also guided by the imaging results. In yet another embodiment, when a cell generation step is determined to have failed, the cell generation process is early terminated based on image guided decision.

The embodiment of the invention discloses a composition, a preparation method and an application of the composition in preparation of medicines, health-care products and foods for treating pet joint diseases. The composition comprises glucosamine, chondroitin sulfate, dimethyl sulfone, green mussels, haematococcus pluvialis, frankincense, curcumin, coix seeds and rhizoma smilacis glabrae. The composition fundamentally inhibits and relieves arthritis from the aspects of eliminating free radicals, neutralizing free radicals, inhibiting proinflammatory cell generation and the like, supplements necessary nutrient substances for cartilage cell growth, promotes synthesis of new cartilage, and achieves the effect of treating both symptoms and root causes in combination with traditional Chinese medicine components for rapid detumescence and pain relief.

The invention discloses a drug-tolerant glioblastoma cell line of primary temozolomide and bevacizumab, a construction method and application of the cell line. The construction method comprises the following steps: obtaining tissue specimens of patients with drug-resistant glioblastoma cell of primary temozolomide and bevacizumab through clinical screening, primarily culturing tumor cells through an enzyme digestion method, and continuously extending generation to 50 generations to obtain a strain of drug-tolerant glioblastoma cell line of primary temozolomide and bevacizumab; the microbial preservation number of the drug-tolerant glioblastoma cell line of primary temozolomide and bevacizumab is CGMCC No.8205. The glioblastoma cell line, disclosed by the invention, is stable in characteristics, stably heritable and high in tolerance to temozolomide and bevacizumab; the cell generation cycle lasts about 48 hours; the glioblastoma cell line has strong invasiveness and tumorigenicity. The glioblastoma cell line constructed by the construction method has important application prospect in aspects of screening medicines for treating glioblastoma and researching nosogenesis of the glioblastoma, and the like.

To date, no immune tolerance agent or combination of immune tolerance agents has been able to sustain insulin-independence among type 1 diabetes patients. This patent provides methods and pharmaceutical compositions for providing insulin independence among newly diagnosed and existing type 1 diabetes. Methods include utilization of PPIs, which increase gastrin resulting in the transformation of human ductal tissue into insulin-secreting new beta cells, used in combination with an immune tolerance agent to protect the new insulin-producing beta cells generated by the PPI from immune destruction. Compositions and methods are provided for beta cell generation therapy comprising at least one member from a group of PPIs with formulations selected from immune tolerance agents, when used in combination result in insulin-independence among new and existing type 1 patients whom currently require insulin to sustain life. Compositions and methods are provided for insulin-independence among type 2 patients using PPIs when combined with therapeutic agents utilized for the treatment of type 2 diabetes.

The invention discloses a method for constructing a pancreatic cancer cell line by positive expression of Chinese B7-H5. The method comprises the following steps of 1, collecting of a specimen; 2, preparing of an Organoid type organ complete culture medium; 3, digestion of tissues; 4, constructing and culture of Organoid type organs of pancreatic cancer; 5, cell generation pass and culture. The method has the beneficial effects that the biology features of the constructed Chinese pancreatic cancer cell line sipanc-1076 are used as pancreatic ductal adenocarcinoma; the particular characteristicof positive expression of B7-H15 which does not exist in other commercialized human pancreatic cancer cell lines is realized; the tumor forming property is good; the method can be applied to the study on tumor immune escape of pancreatic cancer, analysis of related genealogy of Chinese pancreatic cancer, and the like.

Among the various aspects of the present disclosure is the provision of methods, synthetic DC, and compositions for T cell activation. The present disclosure provides for synthetic dendritic cells (DCs), methods of generating synthetic dendritic cells (DCs), methods of generating T cell-encapsulated gelatin microspheres and microcapsules, methods of activating T cells using synthetic DCs, methods for expanding T cells against individualized antigen-specific mutational antigens using synthetic DCs, and methods of treating a chronic disease (e.g., HIV, HPV) or cancer using the synthetic DCs.

The invention discloses a building method of poplar embryonic cell generating technical system, which comprises the following steps: 1. cutting nursery-grown plant with seedling age at 30-50d and seedling height at 4-5cm into 1-2mm as explant; 2. seeding on the XF culture medium to removing differentiation for 100-140h; 3. switching the callus on the Y-2 culture medium; differentiating 72-96h. The invention shortens wood living period, which cultures poplar embryonic cell with totipotence to help poplar breed.