119 results about "Ultrasonic stimulation" patented technology

The invention relates to the technical field of nerve probes, and provides a four-mode integrated nerve probe and a preparation method thereof. The four-mode integrated nerve probe provided by the invention comprises a flexible substrate, an electric signal recording module, an ultrasonic stimulation module, a light stimulation module and a microfluidic channel. The electric signal recording module is used for recording electroneurographic signals, the ultrasonic stimulation module is used for ultrasonic stimulation, the optical stimulation module is used for optical stimulation and signal recording, and the microfluidic channel is filled with drugs through a microfluidic channel control port in the probe body and releases the drugs through a microfluidic channel outlet in the needle tip. According to the four-mode integrated nerve probe provided by the invention, multiple functions are integrated into one probe, so that comprehensive detection and regulation of a nervous system are realized, and the technical problems that a traditional nerve probe is single in function, inflammation is caused by a rigid material and multi-mode integration is insufficient are solved.

The invention relates to a method for promoting nerve cell proliferation and differentiation based on ultrasonic stimulation and application, and belongs to the technical field of biomedicine. According to the method, under the conditions that the power is 90-110 mW and the frequency is 0.9-1.1 MHz, nerve cells are continuously stimulated for 3 days by ultrasonic waves once per day, and each time lasts for 2-4 min. The purposes of increasing the nerve cell proliferation speed and promoting nerve cell differentiation are achieved only through ultrasonic stimulation under specific conditions for the first time, no other substances are added, non-invasion and non-contact are achieved, and toxic and side effects on cells are low; the nerve cells are not subjected to invasive or modified operation; the method has a wide application prospect only through physical conditions.

The invention discloses an ear acupoint closed-loop ultrasonic stimulation system and method based on sleep electroencephalogram signal feedback, and belongs to the technical field of ultrasonic stimulation nerve regulation and control. The ear acupoint closed-loop ultrasonic stimulation system comprises an electroencephalogram acquisition module, a regulation and control module, an ultrasonic transmitting module and a dynamic association module; the electroencephalogram acquisition module is used for acquiring electroencephalogram signals within limited time specified by a user according to specified sampling frequency, amplifying and filtering the acquired signals and sending the processed signals to the regulation and control module; the regulation and control module judges the current sleep stage of the user, drives the ultrasonic transmitting module, performs variable parameter closed-loop ultrasonic stimulation on the user, and automatically adjusts stimulation parameters and duration according to the current sleep stage; and the dynamic association module sets a multi-threshold triggering mechanism based on the real-time electroencephalogram characteristic parameters and discriminates a specific physiological status. According to the invention, accurate acupoint stimulation can be realized, a real-time feedback mechanism is provided, and the accuracy and effectiveness of stimulation can be ensured.

The invention discloses a transcranial ultrasonic stimulation device and a focal length adjusting method, and relates to the technical field of medical instruments and ultrasonic therapy. The screwing assembly is arranged on the outer side of the bottom of the ultrasonic transducer, and the ultrasonic transducer is rapidly disassembled and assembled through the screwing assembly; and the ultrasonic coupling assembly is arranged at the bottom of the screwing assembly and is matched with the ultrasonic transducer, and the ultrasonic coupling assembly is used for reducing air gaps during fitting so as to reduce the sound energy loss. Through the cooperative arrangement of the ultrasonic transducer, the screwing assembly and the ultrasonic coupling assembly, accurate ultrasonic conduction can be achieved, the device can adapt to a complex head shape, especially in complex target spots such as binaural areas, the flexible characteristic of the soft bag ensures that the soft bag is tightly attached to the head tissue, and the effect that the soft bag is tightly attached to the head tissue is achieved. Consistent sound wave conduction can be provided, interference of air or other substances is avoided, and the treatment effect is remarkably improved.

The invention discloses a self-adaptive closed-loop ultrasonic stimulation system and method based on Actor-Critic reinforcement learning, and belongs to the field of neural regulation and control technologies and biomedical engineering, and the system comprises a signal collection module, a preprocessing module, an Actor strategy module, a Critic value module, an ultrasonic stimulation module, a safety monitoring module and a storage module. The Actor strategy module generates safe and controllable ultrasonic stimulation parameters according to the preprocessed electroencephalogram data; the safety monitoring module is used for checking whether the ultrasonic stimulation parameters generated by the Actor module meet safety standards or not; the ultrasonic stimulation module sends out an ultrasonic stimulation signal according to the ultrasonic stimulation parameter; and the Critic value module evaluates the electroencephalogram feedback effect after ultrasonic stimulation and provides feedback to the Actor module for optimizing the experimental strategy of the next step. The invention aims to intelligently adjust the ultrasonic stimulation parameters through the biofeedback data monitored in real time in combination with the reinforcement learning algorithm.

The invention discloses a multifunctional transcranial therapeutic apparatus and a control method thereof. The transcranial therapeutic apparatus relates to the field of medical equipment and solves the problem of single function of a traditional transcranial therapeutic apparatus. The transcranial electrical stimulation module can transmit direct current stimulation or alternating current stimulation; the transcranial magnetic stimulation module can provide a pulsed magnetic field; the ultrasonic stimulation module generates ultrasonic waves through an ultrasonic transducer. The multiple stimulation heads are used for being distributed in different areas of the head, an electrical stimulation electrode slice of the transcranial electrical stimulation module, a magnetic stimulation coil of the transcranial magnetic stimulation module and an ultrasonic transducer of the ultrasonic stimulation module are integrated in each stimulation head, and signal generators of all the modules are controlled in a unified mode through the main controller. A single stimulation mode or combined work of multiple stimulation modes is selected according to the illness state and treatment requirements of a patient, the different stimulation combination modes synergistically act on a target brain area, the treatment effect is improved, and the problems that a traditional transcranial therapeutic apparatus only supports a single stimulation mode and is poor in treatment effect are solved.

The preparation method comprises the following steps: by taking an oriented polylactic acid nanofiber membrane as a raw material, carrying out homogenization treatment to obtain polylactic acid piezoelectric short fibers; gelMA is used as a skeleton matrix, polylactic acid short fibers, LAP and DRG cells are compounded, a 0 / 90-degree alternate grid structure scaffold is constructed through pneumatic extrusion type biological printing, and the dual-network piezoelectric biological scaffold is formed through blue light crosslinking curing. The piezoelectric effect of polylactic acid short fibers is activated through ultrasonic stimulation, mechanical energy is converted into local electric signals, loaded DRG cells are directly driven to secrete CGRP in situ, macrophages are dynamically regulated and controlled to be polarized to M2 anti-inflammatory phenotypes, nerve-immune microenvironment disorder of diabetic bone defects is corrected, vascularization and bone regeneration are promoted, and the diabetic bone defect treatment effect is improved. The problems of short delivery half-life period of traditional cell factors, difficulty in nerve function reconstruction and chronic inflammation caused by non-degradable piezoelectric materials are solved, and non-invasive and degradable precise bone repair is realized.

The invention relates to the technical field of ultrasonic medical instruments, and discloses a wide-area closed-loop control transcranial ultrasonic stimulation system, which comprises an ultrasonic circuit and an ultrasonic transducer, and is characterized in that the ultrasonic circuit is composed of a power supply, a main control circuit, an ultrasonic driving circuit, a matching circuit and a sampling circuit; the power supply is used for adjusting a direct-current input voltage value of the ultrasonic driving circuit according to a control signal of the main control circuit; the matching circuit is used for adjusting a matching inductance value connected in series into the circuit; the sampling circuit is used for sampling and measuring the direct-current input voltage value of the ultrasonic driving circuit and the output voltage applied to the two ends of the ultrasonic transducer. According to the transcranial ultrasonic stimulation system, the output of the ultrasonic transducer can be adjusted in a wide sound intensity range, so that the system can adapt to more application scenes; and meanwhile, the required sound intensity is automatically adjusted by adopting a closed-loop algorithm, so that preset parameters are reduced, the adaptability of an ultrasonic circuit and an ultrasonic transducer is wider, and the use cost is lower.

The invention relates to the field of treatment ultrasound, in particular to a heart rate regulation and control system based on focused ultrasound and application of the heart rate regulation and control system. The heart rate regulation and control system based on focused ultrasound comprises an ultrasonic stimulation module, a central control module and an electrocardiosignal acquisition module, the ultrasonic stimulation module is in communication connection with the central control module, and the electrocardiosignal acquisition module is in communication connection with the central control module. The heart rate regulation and control system based on focused ultrasound provided by the invention is controlled by the multi-degree-of-freedom mobile platform to noninvasively convey ultrasonic energy to a heart target region, so that the following effects are achieved: 1) the heart rate can be noninvasively and safely regulated; 2) the system is simple to operate; 3) the heart rate regulation and control effect is high in real-time performance; and 4) the heart rate regulation and control effect is obvious, and the heart rate can be increased by 10-40%.

The invention discloses MoSe2 nano-enzyme-selenium-rich probiotic composite hydrogel and a preparation method and application thereof, the composite hydrogel comprises a hydrogel matrix and functional components dispersed in the hydrogel matrix, and the functional components comprise anti-MoSe2 nano-enzyme and selenium-rich probiotics wrapped by an antioxidant film. According to the MoSe2 nano-enzyme-selenium-enriched probiotic composite hydrogel provided by the invention, the sonodynamic characteristics of the MoSe2 nano-enzyme and the biological activity of the selenium-enriched probiotics are perfectly fused: under ultrasonic stimulation, the MoSe2 nano-enzyme not only can directly and efficiently generate reactive oxygen species (ROS), but also can catalyze low-concentration hydrogen peroxide to generate a large amount of hydroxyl radicals (. OH); broad-spectrum killing of various pathogenic bacteria is achieved, and the risk of drug resistance evolution of bacteria is reduced from the source; meanwhile, antibiotics secreted by the selenium-enriched probiotics can form a synergistic sterilization effect, so that the pathogenic bacterium removal effect is further enhanced.

The invention relates to the technical field of biological materials, and discloses electrical stimulation LDH hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a zinc salt, a vanadium salt and urea in water in a nitrogen atmosphere to obtain a precursor solution; the precursor solution is subjected to a hydrothermal reaction, and ZnV-LDH is obtained; under stirring, gradually dropwise adding methacrylic anhydride into the gelatin solution, and heating for reaction; adding preheated water into the reaction liquid to stop the reaction; dialyzing and purifying the reaction liquid, pre-freezing, and freeze-drying to obtain hydrogel; and adding ZnV-LDH into the hydrogel, and carrying out ultrasonic mixing, so as to obtain the electrical stimulation LDH hydrogel. Through the synergistic effect of the ZnV-LDH and the hydrogel, the electrical stimulation LDH hydrogel disclosed by the invention has electrical activity and can generate potential change in response to external stimulation; under ultrasonic stimulation, concentration-dependent electrical response can be generated, the response has periodicity and stability, and osteogenic differentiation and cell mechanical properties can be promoted to the maximum extent.

This application relates to the technical field of data analysis and processing, and discloses a method and device for regulating sleep-wake circadian rhythm disorders based on ultrasonic stimulation. The method includes: collecting users' sleep physiological data to construct a feature matrix; analyzing the sleep stage to obtain the disorder type; generating an ultrasonic stimulation parameter scheme; determining the brain region stimulation coordinates to generate spatial positioning data; predicting the user's feedback through a convolutional neural network; comparing the prediction with the actual feedback to adjust the scheme, and obtaining an optimized target stimulation scheme. This application realizes the accurate identification of sleep-wake circadian rhythm disorders, the generation of personalized stimulation parameters, the precise brain region positioning, the predictive evaluation, and the dynamic scheme optimization, thereby improving the regulation effect of ultrasonic stimulation on various sleep-wake circadian rhythm disorders.

A biomimetic intervertebral fusion device with a piezoelectric multifunctional coating belongs to the field of implantable medical devices. It includes an upper piezoelectric multifunctional coating, a fusion device body, and a lower piezoelectric multifunctional coating. A bone graft window is located in the middle of the biomimetic intervertebral fusion device. The piezoelectric multifunctional coating is made of electrospun piezoelectric PVDF / HA fibers, which are conformally adhered to the upper and lower surfaces of the fusion device body through a polydopamine adhesive layer. The upper and lower surfaces of the fusion device body have a biomimetic curved surface configuration to match the morphology of the vertebral endplates, increasing the contact area and reducing stress concentration at the fusion interface. While maintaining an elastic modulus similar to natural bone and good radiation permeability, this fusion device, after implantation, can create a microenvironment conducive to cell adhesion and proliferation. Under the influence of cell traction, ultrasonic stimulation, and the physiological load of the human lumbar spine, it generates electrical stimulation signals, promoting bone fusion, improving the stability of the fusion interface, and reducing the risk of postoperative loosening and subsidence of the fusion device.

The utility model relates to the technical field of oral treatment, and discloses ultrasonic tooth orthodontic auxiliary treatment equipment and an oral treatment device, a denture base body is provided with a treatment part which can be selectively attached to gingiva, tooth covering grooves with different sizes can be formed by fitting gum shapes of different patients, the treatment part can be recycled, and the treatment effect is good. The universality of the ultrasonic tooth orthodontic auxiliary treatment equipment is improved, the treatment cost is reduced, the fit degree of the treatment part and the periodontium is higher, and the orthodontic auxiliary treatment effect is stabilized; the flexible circuit board is arranged in the treatment part and deforms together with the treatment part, conductor wiring used for transmitting electric energy is arranged in the flexible circuit board to integrate circuits, additional wiring is not needed, and wiring harness arrangement is neat; the ultrasonic transduction sheets which are in one-to-one correspondence with the teeth to be orthodontic and are arranged opposite to the gingiva are arranged in the treatment part and are electrically connected with the conductor wiring at the same time, assembly is simple, the ultrasonic transduction sheets are conveniently arranged in the oral cavity of a patient, ultrasonic stimulation is carried out on targeted parts of the teeth, and the orthodontic effect is improved.

The invention belongs to the technical field of medicines, and relates to injectable piezoelectric hydrogel for a spinal cord injury part as well as a preparation method and application of the injectable piezoelectric hydrogel. According to the hydrogel, methacrylated gelatin is used as a carrier, and a piezoelectric material MOF (at) PDA (at) M0, namely MP (at) M, of an enveloping porous structure is loaded in the hydrogel. The hydrogel can achieve dual functions under the ultrasonic action: (1) MP (at) M has piezoelectricity, ultrasonic mechanical energy is converted into electrical stimulation, calcium ion inward flow of astrocytes is triggered, mitochondria is promoted to be transferred from the astrocytes to neurons, and the mitochondrial function of the neurons is recovered; (2) the CD73 enzyme carried by the microglial cell membrane coated on the surface of the MP (at) M can degrade pro-inflammatory ATP (adenosine triphosphate) at the damaged part to generate adenosine, so that astrocytes are promoted to release lactic acid, and rapid energy support is provided for neurons. Mitochondrial transfer and energy supply are synchronously promoted through ultrasonic stimulation, neuronal plasticity is remodeled, spinal cord axon regeneration is promoted, and a new strategy is provided for spinal cord injury treatment.

The invention relates to the technical field of medical data processing and three-dimensional modeling, and discloses a skull thickness distribution diagram construction method and system fusing ultrasonic thickness measurement and optical localization. According to the method, an ultrasonic probe is used for obtaining a skull thickness value at a measuring point of the scalp of a subject, and meanwhile, a three-dimensional space coordinate of the measuring point is accurately determined through an optical navigation positioning system. The system binds the thickness value and the space coordinates in real time to form thickness-space data points, and performs multi-point repeated acquisition on the scalp surface, thereby constructing a three-dimensional thickness point cloud. And finally, the upper computer system performs interpolation processing on the point cloud data to generate a skull thickness distribution diagram with spatial continuity. According to the method, the defect that the skull thickness data and the anatomical space positioning information are mutually independent is overcome, accurate space-time correspondence of the measured value and the space position is realized, an individualized skull model can be constructed quickly at low cost, and an accurate sound field compensation calculation basis is provided for applications such as transcranial ultrasonic stimulation.

The present application relates to the technical field of biological cell culture, and particularly relates to a fixed bed device and method for improving secretion and expression of exosomes. The device comprises a fixed bed reactor, an ultrasonic generator, a connecting flange and a control unit. The ultrasonic generator is installed on the top of the fixed bed reactor through the connecting flange. The control unit is configured to control the ultrasonic generator to apply ultrasonic stimulation to the cells intermittently in the whole cycle of cell culture. The ultrasonic intensity is dynamically regulated in three stages according to the culture cycle. The present application uses pure physical stimulation without adding chemical reagents. Under the premise of maintaining normal growth and activity of cells, the yield of exosomes can be increased by more than 169%. The present application is suitable for animal cells and plant cells and has a wide industrial application prospect. The technical problems of low yield of exosomes, stimulation mode that may affect cell activity or introduction of chemical reagents that may interfere in the prior art can be effectively solved.

The present invention discloses an intraocular irrigation solution containing niacinamide. Niacinamide serves as an active substance in the intraocular irrigation solution, which has the effects of improving the function of corneal endothelial cells, inhibiting cell apoptosis and cell fusion, and improving the mitochondrial damage condition, thereby alleviating or inhibiting corneal edema, reduction of corneal endothelial cells, abnormal corneal transparency, abnormal corneal thickness, abnormal corneal endothelial cell density, abnormal corneal endothelial cell morphology, and abnormal expression of corneal endothelial cell functional proteins caused by ultrasonic stimulation, heat stimulation or oxidative damage to corneal endothelial cells during phacoemulsification surgery.

The application relates to application of a piezoelectric material in preparation of a medicine for treating gout, characterized in that a medicine active ingredient of the medicine comprises the piezoelectric material, the piezoelectric material is barium titanate; the application comprises the following steps: delivering the medicine to a lesion site, applying ultrasonic stimulation to the lesion site, and exciting in-vivo active species degradation of uric acid by the medicine. The piezoelectric material has good piezoelectric catalytic performance, excellent tissue penetration ability, low toxicity and high precision, active species can be controllably excited by means of ultrasonic action at the time of gout attack, uric acid can be efficiently degraded into allantoin which is good in solubility and non-toxic, symptoms can be rapidly relieved, the piezoelectric material can be long-term resident in joints, can keep stable catalytic effect in multiple cycles, and long-term treatment of gout can be realized by single injection, and a new gout treatment scheme which is more effective and more durable is provided.

1. Name of the product in this design: Neck stimulator. 2. Purpose of this design: A stimulator for ultrasonic stimulation of cervical nerves. 3. The key design feature of this product is its shape. 4. The picture or photo that best illustrates the key design points: Design 1 3D diagram 1. 5. Design 1 is designated as the basic design.

A beauty device using skin impedance measurement and ultrasonic cross-frequency according to an embodiment of the present invention comprises: an electrical stimulator including an electrode, which transmits a current to the skin of a subject in order to measure the skin impedance of the subject and care for the skin condition of the subject analyzed on the basis of the skin impedance of the subject, and an LED, which irradiates the skin of the subject with light while the current is being transmitted to the skin of the subject; and a cross-frequency ultrasonic stimulator that measures the skin impedance of the subject and is provided with an ultrasonic generator that irradiates the skin of the subject with ultrasonic waves of cross-frequency in order to care for the skin condition of the subject analyzed on the basis of the skin impedance of the subject, wherein the beauty device can apply electrical stimulation, optical stimulation, and ultrasonic stimulation to the skin of the subject.

SLA-based additive manufacturing using radiation-curable foams enables the production by 3D printing of lightweight parts having desirable physical and functional attributes. A representative method of manufacturing such items includes processing a radiation-curable resin in liquid form to produce a stable, non-aqueous radiation-curable foam, on-demand deposition of the foam at a build layer of a build surface within a 3D printer, and then curing the non-aqueous radiation-curable foam to produce a layer of a 3D build item. Processing the resin typically includes agitation, mixing, shaking, gas injection, ultrasonic stimulation, or combinations thereof. 3D articles of manufacture made by the above-described manufacturing process are also provided.

To solve the problem that a patient cannot be treated or treatment efficiency is reduced due to discomfort and stress to a burn based on heat generation of a probe.SOLUTION: An ultrasonic stimulation apparatus including a main body unit, a probe that outputs an ultrasonic wave oscillated by an ultrasonic transducer through a cover in which the ultrasonic transducer is disposed, and an electric floor pad that supplies negative charges, in which the main body unit includes an electric signal generation unit that outputs an ultrasonic wave supply electric signal, a negative charge circuit unit that outputs a negative charge supply electric signal, and a control unit that controls the electric signal generation unit and the negative charge circuit unit, and the electric signal generation unit can output a first electric signal of a first frequency and a second electric signal of a second frequency, the control unit controls output of the second electric signal and the negative charge supply electric signal on the basis of information indicating that a current of the first electric signal exceeds or falls below a threshold.SELECTED DRAWING: Figure 1

The invention relates to the field of biological medicine, in particular to a retina organ with a vision improving function and a preparation method and application thereof. According to the method, the bottleneck in the prior art is broken through through innovative integration of low-intensity ultrasonic stimulation and a retina organoid technology, the advantages of efficiently accelerating differentiation and maturation of the photoreceptor and improving the differentiation efficiency of the photoreceptor are achieved, meanwhile, the bottleneck of transplantation integration is broken through, survival and integration of the graft are improved, and the purpose of recovering vision is achieved. In conclusion, the retina organoid culture method capable of promoting differentiation of the photoreceptor through ultrasonic stimulation is constructed for the first time, and a new view angle can be provided for research on treatment of retina degenerative diseases. Meanwhile, the core bottlenecks of insufficient photoreceptor differentiation and low transplantation integration rate of the retinal organ are solved, the retinal organ subjected to low-intensity ultrasonic stimulation can be used as a high-quality graft source, and a novel treatment strategy is provided for retinal degenerative diseases.

This invention provides an antibacterial and repair-promoting piezoelectric film, its preparation method, and its application, belonging to the field of piezoelectric material technology. The method involves in-situ self-assembly of Fe-MOF on the surface of BTO, followed by further loading with DHA to synthesize an electrically controlled drug-release, antibacterial, and repair-promoting nanocomposite, BTO / Fe-MOF / DHA. BTO / Fe-MOF / DHA is then added to the surface of semi-cured PDMS to form an antibacterial and repair-promoting piezoelectric film, PDMS-BTO / Fe-MOF / DHA. When the PDMS-BTO / Fe-MOF / DHA piezoelectric film is subjected to ultrasonic stimulation, BTO converts mechanical energy into electrical energy, releasing Fe from the Fe-MOF... 3+ Reduced to Fe 2+ Fe-MOF degrades and releases DHA, and ultimately, Fe... 2+ Reacting with synchronously released DHA, it produces a large amount of toxic ROS, which damages proteins and nucleic acids, inducing bacterial death. The antibacterial and repair-promoting piezoelectric membrane of the present invention generates a piezoelectric potential during mechanical deformation caused by animal movement and induces EF at the wound site to promote wound healing. This antibacterial and repair-promoting piezoelectric membrane shows great potential in the future treatment of bacterial infected wounds.

The application discloses a kind of attenuated salmonella load adjuvant nano combination medicine, the combination medicine is modified to bacterial surface by means of amino and carboxyl condensation reaction, adamantane is simultaneously modified to adjuvant nano medicine surface using diselenium dynamic bond, then the host-guest interaction of adamantane and cyclodextrin is used to assemble two, and then nano medicine is stably combined on the surface of attenuated salmonella.Diselenium dynamic bond can be broken by ultrasound response, so as to realize the effect of drug release.This combination medicine has the tumor targeting enrichment ability of bacteria and the release characteristics of adjuvant nanoparticles under the action of ultrasonic wave, can realize targeted delivery and deep penetration in tumor tissue, and obtain significant tumor inhibition effect under ultrasonic stimulation.The experimental results show that the combination medicine exhibits good therapeutic effect in inhibiting tumor growth, and has excellent biological safety.

The invention relates to the technical field of biology, in particular to acoustoelectric Ba0. 85Ca0. 15Ti0. 9Yb0. 1O3 nanoparticles capable of triggering post-translational modification as well as a preparation method and application of the acoustoelectric Ba0. 85Ca0. 15Ti0. 9Yb0.1 O3 nanoparticles. Stirring and mixing the titanium tetrachloride solution and the mixed reagent solution to obtain a stirred mixed solution; mixing the stirred mixed solution with sodium hydroxide and polyvinylpyrrolidone, and performing heat treatment to obtain a heat-treated substance; and carrying out solid-liquid separation on the heat-treated substance to obtain a solid, washing the solid to be neutral, and drying. The nanoparticles provided by the invention consume H < + > near mitochondria under ultrasonic stimulation, so that mitochondrial membrane potential depolarization is caused. After mitochondrial membrane potential depolarization, E3 ubiquitin ligase Parkin is transferred from cytoplasm to a mitochondrial outer membrane, and is massively gathered and accumulated on the mitochondrial outer membrane, so that ubiquitination modification and cascade degradation of mitochondrial outer membrane anti-apoptosis protein MCL-1 are caused, and cell apoptosis is promoted.

The invention relates to a piezoelectric hydrogel for promoting cartilage regeneration by ultrasonic stimulation and a preparation method and application thereof, the piezoelectric hydrogel is multifunctional hydrogel composed of polyacrylic acid (PAA), chitosan (CS) and potassium sodium niobate (KNN) nanoparticles, the polyacrylic acid (PAA), the chitosan (CS) and the KNN nanoparticles are formed by stirring polyvinyl alcohol (PVA) and acrylic acid (AA) and then carrying out polymerization reaction, the PVA, the PAA, the CS and the KNN nanoparticles are mixed, and the mixture is subjected to ultrasonic stimulation to form the piezoelectric hydrogel. The piezoelectric hydrogel is prepared by physical mixing and chemical crosslinking methods. The piezoelectric hydrogel disclosed by the invention shows excellent biocompatibility, biodegradability, bioadhesion, injectability, thermosensitivity and piezoelectric property, and can generate an electric signal under ultrasonic stimulation to induce surrounding stem cell aggregation and effectively promote cartilage regeneration.

The present invention discloses a real-time ultrasonic stimulation electric signal recording chip and a preparation method thereof, where the preparation method includes the following steps: S1 manufacturing an interdigital electrode on a piezoelectric substrate to obtain a surface acoustic wave chip, and manufacturing a recording electrode and an electrode lead; S2, manufacturing an insulation protection layer on the chip obtained in the S1, and processing the insulation protection layer to form the recording electrode, so as to obtain a chip combining the interdigital electrode and the recording electrode; S3, preparing a PDMS cavity; and S4, bonding the PDMS cavity prepared in the S3 and the chip obtained in the S2. In the present invention, combining the interdigital electrode generating a surface acoustic wave ultrasound with a multi-channel recording electrode, such that real-time recording of a multi-channel electric signal under ultrasonic stimulation is achieved.

The application relates to an ultrasonic stimulation desynchronization simulation method, system and device based on a double-neuron model. The method comprises the following steps: acquiring ultrasonic wave data, wherein the ultrasonic wave data comprises sound pressure and / or frequency; inputting the ultrasonic wave data into an intramembranous cavitation model constructed based on an intramembranous cavitation effect to obtain an intramembranous capacitive displacement current; inputting the obtained intramembranous capacitive displacement current into a double-neuron model to obtain time corresponding to the peak values of two neuron potentials; calculating a phase difference according to the time corresponding to the peak values of the two neuron potentials; and obtaining that the two neurons are in a desynchronized state or a synchronized state according to the phase difference. The method aims to realize desynchronization based on ultrasonic stimulation simulation of the double-neuron model, and to explore the inhibition capacity and potential application value of the double-neuron model on morbid attacks.