Ultrasonic stimulation device and ultrasonic stimulation method for promoting proliferation and differentiation of spinal cord-derived neural stem cells

A neural stem cell and stimulation device technology, applied in tissue cell/virus culture devices, nervous system cells, stress-stimulated microbial growth methods, etc., to achieve the effects of preventing pollution, promoting proliferation and differentiation, and having a wide range of applications
CN110577896APending Publication Date: 2019-12-17SHANGHAI TONGJI HOSPITAL +1
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
SHANGHAI TONGJI HOSPITAL
Publication Date
2019-12-17

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Abstract

The invention relates to an ultrasonic stimulation device and ultrasonic stimulation method for promoting proliferation and differentiation of spinal cord-derived neural stem cells. The device is provided with a power supply, a driving signal generator, a pulse repetition frequency generator, a power amplifier, an ultrasonic probe and a hydrophone, wherein the driving signal generator converts a common current into a high-frequency current, the pulse repetition frequency generator generates a pulse signal with a repetition frequency from the high-frequency current, the power amplifier amplifies the pulse signal, and finally the ultrasonic probe emits an ultrasonic wave from the amplified pulse signal, the hydrophone is used for receiving acoustic signals in liquid, the ultrasonic probe iscylindrical and an isolation vessel is arranged above the ultrasonic probe. According to the method, primary cultured spinal cord-derived neural stem cells are subjected to ultrasonic stimulation at an intensity of 10-150 mW / cm2. The method is suitable for researching influence of ultrasound on the proliferation and differentiation of the spinal cord-derived neural stem cells. The device utilizeslow-frequency pulse ultrasound to stimulate the spinal cord-derived neural stem cells, promotes the proliferation and differentiation of the spinal cord-derived neural stem cells, and further promotesrepair of spinal cord injury.
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Description

technical field

[0001] The invention relates to the technical field of nerve regeneration and repair promoted by physical factors, in particular to an ultrasonic stimulation device and method capable of promoting the proliferation and differentiation of spinal cord-derived neural stem cells. Background technique

[0002] Spinal cord injury (SCI) usually refers to the direct injury of the spinal cord caused by various reasons such as trauma, resulting in the loss of motor and sensory functions below the level of injury to varying degrees, and even complete loss of motor and sensory functions leading to paraplegia. SCI has a high morbidity rate. In addition to causing quadriplegia and hemiplegia, it may also produce other secondary diseases, which seriously affect the quality of life of patients. The treatment and rehabilitation of SCI has always been one of the major problems in the field of basic and clinical medicine.

[0003] Ultrasound is a mechanical wave with a sound s...

Claims

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