Ultrasonic stimulation device and ultrasonic stimulation method for promoting proliferation and differentiation of spinal cord-derived neural stem cells

A neural stem cell and stimulation device technology, applied in tissue cell/virus culture devices, nervous system cells, stress-stimulated microbial growth methods, etc., to achieve the effects of preventing pollution, promoting proliferation and differentiation, and having a wide range of applications

Pending Publication Date: 2019-12-17
SHANGHAI TONGJI HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no report on whether ultrasound can promote the proliferation and differentiation of neural stem cells and form neural circuits to restore spinal

Method used

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  • Ultrasonic stimulation device and ultrasonic stimulation method for promoting proliferation and differentiation of spinal cord-derived neural stem cells
  • Ultrasonic stimulation device and ultrasonic stimulation method for promoting proliferation and differentiation of spinal cord-derived neural stem cells
  • Ultrasonic stimulation device and ultrasonic stimulation method for promoting proliferation and differentiation of spinal cord-derived neural stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 The present invention can promote the proliferation and differentiation of spinal cord-derived neural stem cells ultrasonic stimulation device (1)

[0038] See figure 1 , figure 1It is a structural schematic diagram of an ultrasonic stimulation device that can promote the proliferation and differentiation of spinal cord-derived neural stem cells in the present invention. The ultrasonic stimulation device is provided with a power supply 1 , a driving signal generator 2 , a pulse repetition frequency generator 3 , a power amplifier 4 , an ultrasonic probe 5 , and a hydrophone 6 . The drive signal generator 2 is a device capable of providing electrical signals of various frequencies, waveforms and output levels. The pulse repetition frequency generator 3 is a PRF generator, which is used to generate a pulse signal with a repetition frequency. The power amplifier 4 is used to generate greater power output. The ultrasonic probe 5 is a probe used in ultrasonic t...

Embodiment 2

[0045] Example 2 The ultrasonic stimulation device of the present invention that can promote the proliferation and differentiation of spinal cord-derived neural stem cells (2)

[0046] See figure 2 , figure 2 It is a structural schematic diagram of another ultrasonic stimulation device that can promote the proliferation and differentiation of spinal cord-derived neural stem cells in the present invention. The ultrasonic stimulation device is provided with a power supply 1 , a driving signal generator 2 , a pulse repetition frequency generator 3 , a power amplifier 4 , an ultrasonic probe 5 , and a hydrophone 6 . The drive signal generator 2 is a device capable of providing electrical signals of various frequencies, waveforms and output levels. The pulse repetition frequency generator 3 is a PRF generator, which is used to generate a pulse signal with a repetition frequency. The power amplifier 4 is used to generate greater power output. The ultrasonic probe 5 is a probe ...

Embodiment 3

[0048] Example 3 The present invention promotes the ultrasonic stimulation method of the proliferation and differentiation of spinal cord-derived neural stem cells

[0049] 1. Low-intensity pulsed ultrasound promotes self-renewal of spinal cord-derived neural stem cells

[0050] In order to study the regulating effect of physical factors on the self-renewal ability of spinal cord-derived neural stem cells by low-intensity pulsed ultrasound, we first conducted the neurosphere formation analysis experiment. We digested the spinal cord-derived neural stem cells isolated from adult mouse thoracic spinal cord into single cells (5*10 4 / mL), the neural stem cells were cultured on polylysine-treated cell slides, and the proliferation medium was used to adhere to the wall for 12 hours, and different intensities (0%, 1%, 2%, 3%, 6% and 13%, corresponding to 0, 10, 20, 30, 150 and 623mW / cm 2 ) pulse ultrasonic treatment, 1MHz longitudinal wave probe, pulse repetition frequency (PRF) 1...

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Abstract

The invention relates to an ultrasonic stimulation device and ultrasonic stimulation method for promoting proliferation and differentiation of spinal cord-derived neural stem cells. The device is provided with a power supply, a driving signal generator, a pulse repetition frequency generator, a power amplifier, an ultrasonic probe and a hydrophone, wherein the driving signal generator converts a common current into a high-frequency current, the pulse repetition frequency generator generates a pulse signal with a repetition frequency from the high-frequency current, the power amplifier amplifies the pulse signal, and finally the ultrasonic probe emits an ultrasonic wave from the amplified pulse signal, the hydrophone is used for receiving acoustic signals in liquid, the ultrasonic probe iscylindrical and an isolation vessel is arranged above the ultrasonic probe. According to the method, primary cultured spinal cord-derived neural stem cells are subjected to ultrasonic stimulation at an intensity of 10-150 mW/cm2. The method is suitable for researching influence of ultrasound on the proliferation and differentiation of the spinal cord-derived neural stem cells. The device utilizeslow-frequency pulse ultrasound to stimulate the spinal cord-derived neural stem cells, promotes the proliferation and differentiation of the spinal cord-derived neural stem cells, and further promotesrepair of spinal cord injury.

Description

technical field [0001] The invention relates to the technical field of nerve regeneration and repair promoted by physical factors, in particular to an ultrasonic stimulation device and method capable of promoting the proliferation and differentiation of spinal cord-derived neural stem cells. Background technique [0002] Spinal cord injury (SCI) usually refers to the direct injury of the spinal cord caused by various reasons such as trauma, resulting in the loss of motor and sensory functions below the level of injury to varying degrees, and even complete loss of motor and sensory functions leading to paraplegia. SCI has a high morbidity rate. In addition to causing quadriplegia and hemiplegia, it may also produce other secondary diseases, which seriously affect the quality of life of patients. The treatment and rehabilitation of SCI has always been one of the major problems in the field of basic and clinical medicine. [0003] Ultrasound is a mechanical wave with a sound s...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/42C12N13/00C12N5/0797C12N5/0793
CPCC12M35/04C12N13/00C12N5/0619
Inventor 程黎明程茜徐峥徐委
Owner SHANGHAI TONGJI HOSPITAL
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