286 results about "Fibroblastic cell" patented technology

The invention belongs to the technical field of bionic nano-drugs, and particularly relates to a bionic vesicle preparation as well as a preparation method and application thereof. The bionic vesicle preparation disclosed by the invention is prepared from the following raw materials: mesenchymal stem cell outer vesicles, nintedanib and DSPE-PEG-HA (hyaluronic acid modified pegylated phospholipid); the nintedanib is loaded in the mesenchymal stem cell outer vesicle, and the DSPE-PEG-HA is coupled to the surface of the mesenchymal stem cell outer vesicle. The bionic vesicle preparation disclosed by the invention can be used for directly delivering a drug to a pulmonary fibrosis injury part through inhalation type drug delivery, and acting on a focus part at a higher local concentration, meanwhile, the exposure of the drug in whole-body circulation is remarkably reduced, the occurrence of side effects is reduced, and the specific targeting intervention and regulation on fibroblasts are realized.

The invention relates to application of a platelet reaction protein 4 in preparation of an alopecia medicine, and belongs to the field of medical biology. The invention aims to solve the technical problems of limited curative effect, obvious side effect and narrow application range of the existing alopecia treatment medicine. The invention provides an application of a platelet reaction protein 4 (THBS4) in preparation of an alopecia medicinal preparation, which can promote hair follicles to be converted from a resting period to a growing period by regulating and controlling a mechanical microenvironment around the hair follicles, enhancing the interaction between fibroblasts and hair follicle stem cells and activating regeneration signal channels in hair papilla and hair bud areas. The traditional Chinese medicine composition breaks through the limitation that existing treatment only aims at specific alopecia types, and can be widely applied to various alopecia diseases such as androgenetic alopecia, pressure alopecia and alopecia after chemotherapy. And compared with the traditional medicine, the traditional Chinese medicine composition has the advantages of quick response, no skin irritation reaction and improved hair follicle regeneration efficiency, and provides a brand new solution for safe and efficient treatment of alopecia.

A cellularized nerve regeneration graft is disclosed that includes an electrospun biodegradable polymer conduit having an exterior surface and an interior luminal space, a plurality of fibroblasts seeded to the exterior surface of the conduit, and a system filling the interior luminal space of the conduit. The system may include a hydrogel matrix or augmented hydrogel matrix and Schwann cells. The cellularized nerve regeneration graft may be used in the repair of peripheral nerve injuries. Methods of making the cellularized nerve regeneration graft are also disclosed.

The invention provides a superfine-diameter PCL / COL nanofiber membrane as well as a preparation method and application thereof, and relates to the technical field of skin wound repair materials. The PCL / COL nanofiber membrane material is prepared from PCL and I-type collagen as raw materials through electrostatic spinning and vacuum drying. According to the preparation method disclosed by the invention, an electrostatic spinning technology is combined with strict regulation and control on parameters, so that the stable and superfine 40-nanoscale PCL / COL nanofiber membrane material is prepared. The diameter of the superfine PCL / COL nanofiber membrane material reaches the bionic scale of the minimum natural collagen of an extracellular matrix, and meanwhile, the superfine PCL / COL nanofiber membrane material shows more excellent hydrophilicity and lower surface roughness, has good biological safety and mechanical stability, not only realizes high matching with a natural ECM hierarchical structure, but also has good application prospects. By enhancing the cell-material interface interaction, the adhesion, proliferation and migration capacities of fibroblasts are remarkably improved, the wound healing period can be effectively shortened, an excellent repair promoting effect is shown, and a solid foundation is laid for clinical transformation of materials.

This disclosure describes the use of azithromycin, roxithromycin, and telithromycin, including derivatives thereof, as senolytic drugs. BrdU was used to induce senescence in model human fibroblast cell lines. Also disclosed are methods for screening compounds for senolytic activity. The SRB assay was used to measure cell viability through protein content. Azithromycin roxithromycin, and telithromycin, clinically-approved pharmaceuticals, were found to be senolytic drugs. However, the closely-related parent compound, erythromycin, showed no senolytic activity. Azithromycin strongly induced both aerobic glycolysis and autophagy in human fibroblasts, but showed bi-phasic effects including on mitochondrial oxygen consumption rates with inhibitory activity at 50 μM and stimulatory activity at 100 μM. The xCELLigence real-time assay system showed that azithromycin preferentially targets senescent cells, removing approximately 97% (nearly a 25-fold reduction in senescent cells).

The invention discloses a tissue engineering integrated trachea regeneration construction method based on a frame structure scaffold and application. A multi-tissue-structure integrated gas pipe is constructed based on a 3D printing bioelastomer frame structure support, and the complex structure and physiological functions of a natural gas pipe are highly simulated. The preparation method comprises the following steps: firstly, by utilizing the characteristic that temperature-sensitive hydrogel Pluronic F-127 becomes semi-solid gel at 37 DEG C and is liquefied at a low temperature of 4 DEG C, and by adopting a strategy that the temperature-sensitive hydrogel is sacrificed after occupying, the patterned distribution of chondrocytes and fibroblasts on a two-dimensional plane is realized; secondly, simulating a three-dimensional tubular trachea structure through a 2D-3D (two-dimensional to three-dimensional) rolling assembly strategy, and realizing the ordered regeneration of C-shaped cartilage ring / O-shaped fiber ring alternate distribution and axial strip-shaped fiber bundle triple structures under the skin of the nude mouse. And finally, successfully extracting the airway mucosa epithelium, separating and amplifying enough airway basal cells in vitro, and loading the airway basal cells to the inner wall of the regenerated trachea by utilizing the photo-crosslinking hydrogel, thereby realizing the function simulation of the airway epithelium and enhancing the physiological functionality.

Provided are immortalized fish fibroblast cells and food products comprising said fish fibroblast cells.

The invention discloses a method for preparing bovine MSTN gene editing cells by using CRISPR / Cas12i, and belongs to the technical field of biology. According to the invention, an sgRNA expression vector aiming at the bovine MSTN gene is constructed, and a CRISPR / Cas12i system is utilized to carry out precise gene editing on a specific target area of a first exon of the bovine primary fibroblast MSTN gene, so that mRNA of the MSTN gene cannot be normally transcribed, and MSTN gene deleted cells are obtained. Compared with other gene editing tools, the Cas12i system shows higher specificity and editing efficiency (improved by 3%-5%), and the off-target effect risk is effectively reduced. The prepared MSTN gene deleted bovine recombinant cell line is stable and efficient in editing efficiency, the cell expression level is not affected, the MSTN gene deleted bovine recombinant cell line can be widely applied to somatic cell cloning and microinjection preparation of double-muscle-hip beef cattle, and a new strategy is provided for beef cattle breeding.

The invention provides a bionic MOF nano platform capable of double targeting cervical cancer tumor cells and cancer-related fibroblasts and co-delivering an FAK inhibitor and bismuth as well as a preparation method and application thereof, the bionic MOF nano platform comprises an MOF material loaded with the FAK inhibitor (IN10018) and bismuth (Bi), and a hybrid membrane coating the outer part of the MOF material loaded with the FAK inhibitor and the bismuth, the hybrid membrane is obtained by mixing and physically extruding a cervical cancer tumor cell (HeLa and SiHa) membrane and a cancer-related fibroblast (CAFs) membrane. According to the bionic MOF nano platform, in an acid environment, the ZIF-8 nano particles loaded with the IN10018 and the Bi disintegrate, the IN10018 is released, CAFs infiltration in tumors is reduced, meanwhile, due to addition of the Bi, the radiation absorption efficiency is improved, and the sensitivity of the tumors to radiotherapy is further enhanced through a double-targeting mechanism.

The invention discloses a preparation method and application of a mesenchymal stem cell exosome-loaded absorbable suture line, noradrenaline is utilized to activate the surface of a collagen suture line, then human umbilical cord mesenchymal stem cell exosomes are assembled to the surface of the activated suture line through a soaking method, and the absorbable suture line with the surface functionalization is constructed. After the suture line is used, the wound healing effect is good, the blood vessel and cell recovery degree is good, proliferation and migration of fibroblasts can be remarkably promoted, proliferation of T lymphocytes and Th1 cells is effectively inhibited, and a powerful guarantee is provided for tissue repair.

The invention aims to provide a preparation method and application of a nano-enzyme hydrogel dressing, and belongs to the technical field of wound repair materials, the method prepares a nano-enzyme Mn-ZIF (at) GOx which is composed of manganese and zinc doped MOF and has multi-enzyme activity, and the nano-enzyme Mn-ZIF (at) GOx is anchored on bacterial cellulose hydrogel. Mn-ZIF (at) GOx / BC shows an antibacterial property independent of ROS (reactive oxygen species) and a relatively strong immunoregulation characteristic. According to the hydrogel, by simulating the cascade process of superoxide dismutase and catalase, hydroxyl free radicals, superoxide anions and hydrogen peroxide are effectively removed, O2 is generated, and M2 polarization of macrophages can be induced, so that inflammation is relieved. MZGB creates a good immune microenvironment through reprogramming macrophages, and proliferation, migration and angiogenesis of fibroblasts and endothelial cells are remarkably promoted through a paracrine mechanism. The MZGB hydrogel disclosed by the invention has the characteristics of resisting inflammation, removing active oxygen, resisting bacteria and promoting angiogenesis, obviously enhances the healing of diabetic wounds, and is simple to prepare.

The present invention provides a biomimetic MOF nanoplatform that can dual-target cervical cancer tumor cells and cancer-associated fibroblasts and co-deliver FAK inhibitors and bismuth, and its preparation method and application. The biomimetic MOF nanoplatform includes a MOF material loaded with FAK inhibitors (IN10018) and bismuth (Bi), and a hybrid membrane coated on the outside of the MOF material loaded with FAK inhibitors and bismuth. The hybrid membrane is obtained by physical extrusion after mixing cervical cancer tumor cell (HeLa and SiHa) membranes and cancer-associated fibroblast (CAFs) membranes. In an acidic environment, the biomimetic MOF nanoplatform of the present invention disintegrates ZIF-8 nanoparticles loaded with IN10018 and Bi, releases IN10018, reduces CAFs infiltration in the tumor, and at the same time, the addition of Bi improves radiation absorption efficiency, further enhancing the sensitivity of the tumor to radiotherapy through a dual-targeting mechanism.

The invention belongs to the technical field of pharmaceutical preparations. The invention discloses a multifunctional bionic nano preparation, a preparation method and application. According to the multifunctional bionic nano preparation, the ginsenoside Rg3 has the dual functions of a medicine and a membrane stabilizer, the platelet membrane has the natural targeting capability on circulating tumor cells and metastases, and meanwhile, the cationic liposome is used for adsorbing negatively-charged NETs nucleic acid protein compounds, so that potential reversal and active targeting are achieved, and the multifunctional bionic nano preparation has a good application prospect. And by co-carrying paclitaxel (PTX) and a photothermal agent (PCP) and integrating photothermal-chemotherapy-immunogenic death (ICD) induction functions, the tumor-related fibroblast activation can be effectively inhibited, circulating tumor cells can be efficiently captured, a tumor immune microenvironment can be remodeled, the tumor cells can be effectively killed, and tumor distal metastasis can be inhibited.

The invention provides a polylactic acid-polycitrate composite microsphere as well as a preparation method and application thereof. The polylactic acid-polycitrate composite microsphere is prepared from the following raw materials: a polycitrate prepolymer and poly-L-lactic acid. According to the polylactic acid-polycitrate composite microsphere provided by the invention, the polycitrate prepolymer is introduced, so that mechanical-degradation double-phase matching of a material can be realized, the hydrophobicity of the poly-L-lactic acid can be reduced, the hydrophilic-hydrophobic balance of the surface of the composite microsphere is realized, the affinity and biological activity of cells are improved, and the biocompatibility of the composite microsphere is improved. And the poly citrate prepolymer and the poly-L-lactic acid are matched for use, so that the whole polylactic acid-poly citrate composite microsphere has bionic viscoelasticity, thereby promoting the spreading of fibroblasts and the secretion of more integrin and collagen monomers, facilitating the behavior regulation and control of cells, and mildly stimulating the regeneration of collagen.

The invention discloses a preparation method of a three-dimensional umbilical cord mesenchymal stem cell exosome and application of the three-dimensional umbilical cord mesenchymal stem cell exosome in the fields of medicines and medical beauty. The preparation method comprises the step of amplifying umbilical cord mesenchymal stem cells (UC-MSCs) by adopting a three-dimensional culture system. Experiments show that IGF-1, EGF, VEGF, TGF-beta, PDGF, FGF and the like in the exosome subjected to three-dimensional culture are beneficial to skin repair and oxidation resistance, and the content of cell active factors for promoting collagen secretion is remarkably increased. The umbilical cord mesenchymal stem cell exosome significantly improves the fibroblast proliferation activity, and UV-induced skin fibroblast damage model construction and function verification experiments also prove that the exosome can promote the HA level recovery of damaged skin fibroblasts. The invention provides an efficient and safe biological material for tissue repair.

The present invention discloses a method for reprogramming fibroblasts into autonomic ganglion organoids. The method uses a TetO-controlled lentiviral system to establish a method for reprogramming mouse somatic cells into autonomic ganglion organoids (induced autonomic ganglion, iAG) using a combination of transcription factors; the experimental results show that the combination of three transcription factors, Ascl1, Phox2a / 2b and Hand2, can induce mouse embryonic fibroblasts to generate self-organized, network-connected neural clusters. The method shows that the three factors Ascl1, Phox2a / 2b and Hand2 are effective means for reprogramming mouse fibroblasts into iAG organoids, and the induced iAG organoids may be used as a model to decipher the pathogenesis of autonomic neurological diseases and screen effective sources of drugs and cell replacement therapy.

The invention discloses a method for enhancing mechanical force of CAR-T cells on target cells to overcome mechanical immune checkpoints of SFs and application, and relates to the technical field of crossing of immunotherapy and biomechanics, in particular to CAR-T cells targeting fibroblast activated protein. The invention discloses a CAR-T (chimeric antigen receptor T-T) cell and SFs (small-form-factor s) DNA (deoxyribonucleic acid) nano connector for connecting the CAR-T cell with the SFs, the formation of a CAR cluster and mechanical force transmission are promoted by shortening the membrane spacing of an immune synaptic interface to 16-28nm, the DNA nano connector is formed by self-assembling complementary tetrahedral DNA structures, and the TDS comprises a single-stranded DNA vertex S4 with the length of 24-58 nucleotides. According to the method for enhancing the mechanical force of the CAR-T cells on the target cells to overcome the mechanical immune checkpoints of the SFs and the application, the escape and killing problems of the soft target cells are solved, the soft target cells are efficiently removed, and the killing rate of the CAR-T cells treated by DNJ-17 on the SFs is remarkably increased.

The present invention relates to a method for producing a skin tissue model, comprising: (a) isolating and culturing fibroblasts and keratinocytes from skin tissue; (b) preparing a bioink composition for a dermal layer and a bioink composition for an epidermal layer, the bioink composition containing the isolated cells; and (c) producing a skin tissue model using the bioink composition for a dermal layer and the bioink composition for an epidermal layer.

The invention relates to a nucleic acid aptamer for targeting CD34 positive orbit fibroblasts, which is characterized in that the nucleotide sequence of the nucleic acid aptamer is as shown in SEQ ID NO: 15. The nucleic acid aptamer provided by the invention has the advantages of high affinity, strong specificity, small molecular weight, good stability, simple synthesis, low cost, easy modification, no immunogenicity and the like, and can meet the requirements of rapid detection of thyroid-related ophthalmopathy at present.

The invention discloses a composition for inducing functional ciRPE cells through chemical reprogramming, an induction method and application. The invention provides a two-step chemical reprogramming strategy, and fibroblasts can be transdifferentiated into functional RPE (ciRPE) cells through an eye domain (EF) / optic bubble (OV) intermediate state. The ciRPE cells are very similar to natural RPE cells in structure and function, have no tumorigenic risk after being transplanted to the retina of an RD rat, can be integrated into host tissues, effectively protect photoreceptor cells and remarkably recover the visual function.

The invention discloses a stably transfected cell strain for evaluating fibroblast autophagy flux and a construction method of the stably transfected cell strain. The construction method comprises the following step: infecting fibroblasts by using HBAD (Hepatitis B Antigen)-red fluorescent protein-EGFP-LC3 autophagy double-labeled adenovirus. The construction method can be combined with a super-resolution microscope to dynamically observe the autophagy intensity change in the primary fibroblasts through an imaging method.

The present invention discloses an in vitro preparation method of human mesenchymal stem cells. The method comprises the following steps: using MSC culture medium supplemented with chemical small molecules to induce iPSC-derived MSCs; after the iPSC cell confluence reaches 40%, the culture medium containing chemical small molecules is replaced; after 8-10 days of induction, the cells are collected and passaged; and the cells are cultured to the P1 generation, showing uniform fibroblast-like, spindle-shaped or fusiform cells. The prepared hiPSC-MSCs meet the relevant phenotypes of mesenchymal stem cells, wherein the positive rates of CD73, CD90 and CD105 are higher than 95%, and the cells have immunomodulatory activity.

The invention discloses tightening and anti-aging massage cream and a preparation method thereof, and belongs to the technical field of cosmetics. The massage cream comprises butanediol, glycerin, allantoin, carbomer, sodium hyaluronate, hydrolyzed collagen, a moistening agent, an emulsifier, a pH regulator, a penetration enhancer, a skin conditioner and an antioxidant. Through scientific and reasonable proportioning, various active ingredients with different effects are compounded, so that the massage cream can directly permeate skin tissues, increase cell viability, promote blood circulation, deeply nourish the skin, comprehensively improve the skin state and remarkably improve the skin compactness; through synergistic cooperation of natural active ingredients such as tetrahydromethylpyrimidinecarboxylic acid, bakuchiol, polypeptide, a plant extract aqueous solution, hydroxypropyl tetrahydropyrantriol and a helichrysum extract, the activity of collagenase can be effectively inhibited, fibroblasts are stimulated to generate collagen, and therefore the skin elasticity is remarkably improved, and meanwhile the purposes of tightening and resisting aging are achieved.

The invention relates to an estrogen receptor alpha and fibroblast activating protein dual-targeting near-infrared fluorescent probe as well as a preparation method and medical application thereof. Specifically, the probe can be used as a fluorescence probe for developing cells co-expressing the estrogen receptor alpha and the fibroblast activating protein, and can also be used as a near-infrared fluorescence imaging probe for diagnosing benign / malignant lesions such as nodules / tumors co-expressing the estrogen receptor alpha and the fibroblast activating protein. The fluorescent probe can also be applied to research on related biological events in cells for co-expressing the estrogen receptor alpha and the fibroblast activating protein, and particularly can be applied to fluorescent navigation in tumor resection operations for co-expressing the estrogen receptor alpha and the fibroblast activating protein; particularly, the composition can be applied to the synergistic treatment of an anti-estrogen therapy and a photodynamic therapy on a diseased region which co-expresses the estrogen receptor alpha and the fibroblast activating protein.

The present application relates to the field of medical supplies, and specifically provides a recombinant keratin injectable gel and a preparation method thereof. The injectable gel can form a recombinant keratin gel with reliable quality without adding a cross-linking agent while maintaining the mass ratio α of keratin and cysteine ​​at 5:1≤α<15:1 and adjusting to an alkaline pH value. The application of the injectable gel in medical products includes soft tissue filling, drug delivery, and cosmetic surgery. The injectable gel can also be used to promote fibroblast proliferation and migration, and promote fibroblast expression and secretion of collagen.

The present invention refers to molecules targeting membrane associated latent-transforming growth factor beta-binding protein 2 (LTBP2). Further the invention relates to molecules targeting LTBP2 for treatment and diagnosis of diseases associated with LTBP2 expressing fibroblasts. In particular, the invention refers to a nucleic acid encoding molecule targeting LTBP2 and a cell expressing the molecule targeting LTBP2.

A cardiac organoid has a first layer that forms an inner part and has cavities. The first layer is at least in part surrounded by a second layer including endothelial cells and cardiomyocytes. The second layer is at least in part surrounded by a third layer including cardiomyocytes and epicardial cells. The third layer is at least in part surrounded by a fourth layer including fibroblast cells.

The invention provides a co-culture system for increasing the yield of endometrial stem cell exosomes and a method for increasing the yield of endometrial stem cell exosomes, firstly, fibroblasts are subjected to mitomycin C pretreatment, so that the cells are stagnated in G1, S and G2 stages of a cell cycle, meanwhile, RNA and protein synthesis continues to be carried out, and the endometrial stem cell exosomes are obtained; the fibroblasts are kept in a non-proliferative but metabolic active state; then, endometrial stem cells extracted from endometrial tissues in the proliferation period and fibroblasts are indirectly co-cultured through a Transwell chamber and are separated through a semi-permeable membrane, and stem cell differentiation caused by inter-cell contact reaction and fusion of the stem cells and somatic cells is eliminated; after co-culture, taking out the endometrial stem cells, putting the endometrial stem cells into a serum-free culture medium special for exosomes for separate culture, harvesting cell supernate, and purifying the exosomes; compared with the endometrial stem cells which are independently cultured in the whole process, the co-culture system disclosed by the invention has the advantages that the amplification speed of the endometrial stem cells and the exosome yield of unit quantity of cells are remarkably improved.

The invention discloses a method for inducing fibroblasts to be reprogrammed into mesenchymal matrix-like cells (MLCs) by using small chemical molecules and application of the mesenchymal matrix-like cells (MLCs) in the anti-aging field. The invention discloses a set of specific chemical small molecule composition and an induction scheme thereof, which can be used for efficiently reprogramming fibroblasts from somatic cells into MLCs. Furthermore, the huge potential of the MLCs obtained by the method in anti-aging research is deeply explored, and the effect of the MLCs is proved to be obviously superior to that of umbilical cord mesenchymal stem cells in the aspects of reversing cell aging phenotypes and recovering tissue functions. The invention not only provides a new stem cell source strategy for avoiding the ethical and safety problems of the traditional reprogramming technology, but also lays a solid cell foundation for developing a novel, safe and effective anti-aging therapy based on cell transplantation or secreted factors.

The invention relates to an application of a synthetic peptide targeting CLDN12 in reduction of hepatic metastasis of pancreatic cancer. Research finds that the tight junction protein Claudin12 (CLDN12) is expressed on the surface of pancreatic cancer cells and also expressed on fibroblasts, and the CLDN12 on the fibroblasts and the CLDN12 on the pancreatic cancer cells mediate an adhesion effect through homologous interaction and guide migration and invasion of the pancreatic cancer cells. The adhesion effect of CLDN12-mediated fibroblasts and pancreatic cancer cells is blocked, and the invasion of the pancreatic cancer cells dominated by the fibroblasts can be blocked, so that the invasion and metastasis of the pancreatic cancer are reduced.