165 results about "Fibroblastic cell" patented technology

Provided are immortalized fish fibroblast cells and food products comprising said fish fibroblast cells.

The present invention provides a biomimetic MOF nanoplatform that can dual-target cervical cancer tumor cells and cancer-associated fibroblasts and co-deliver FAK inhibitors and bismuth, and its preparation method and application. The biomimetic MOF nanoplatform includes a MOF material loaded with FAK inhibitors (IN10018) and bismuth (Bi), and a hybrid membrane coated on the outside of the MOF material loaded with FAK inhibitors and bismuth. The hybrid membrane is obtained by physical extrusion after mixing cervical cancer tumor cell (HeLa and SiHa) membranes and cancer-associated fibroblast (CAFs) membranes. In an acidic environment, the biomimetic MOF nanoplatform of the present invention disintegrates ZIF-8 nanoparticles loaded with IN10018 and Bi, releases IN10018, reduces CAFs infiltration in the tumor, and at the same time, the addition of Bi improves radiation absorption efficiency, further enhancing the sensitivity of the tumor to radiotherapy through a dual-targeting mechanism.

The invention belongs to the technical field of pharmaceutical preparations. The invention discloses a multifunctional bionic nano preparation, a preparation method and application. According to the multifunctional bionic nano preparation, the ginsenoside Rg3 has the dual functions of a medicine and a membrane stabilizer, the platelet membrane has the natural targeting capability on circulating tumor cells and metastases, and meanwhile, the cationic liposome is used for adsorbing negatively-charged NETs nucleic acid protein compounds, so that potential reversal and active targeting are achieved, and the multifunctional bionic nano preparation has a good application prospect. And by co-carrying paclitaxel (PTX) and a photothermal agent (PCP) and integrating photothermal-chemotherapy-immunogenic death (ICD) induction functions, the tumor-related fibroblast activation can be effectively inhibited, circulating tumor cells can be efficiently captured, a tumor immune microenvironment can be remodeled, the tumor cells can be effectively killed, and tumor distal metastasis can be inhibited.

The invention provides a polylactic acid-polycitrate composite microsphere as well as a preparation method and application thereof. The polylactic acid-polycitrate composite microsphere is prepared from the following raw materials: a polycitrate prepolymer and poly-L-lactic acid. According to the polylactic acid-polycitrate composite microsphere provided by the invention, the polycitrate prepolymer is introduced, so that mechanical-degradation double-phase matching of a material can be realized, the hydrophobicity of the poly-L-lactic acid can be reduced, the hydrophilic-hydrophobic balance of the surface of the composite microsphere is realized, the affinity and biological activity of cells are improved, and the biocompatibility of the composite microsphere is improved. And the poly citrate prepolymer and the poly-L-lactic acid are matched for use, so that the whole polylactic acid-poly citrate composite microsphere has bionic viscoelasticity, thereby promoting the spreading of fibroblasts and the secretion of more integrin and collagen monomers, facilitating the behavior regulation and control of cells, and mildly stimulating the regeneration of collagen.

The invention discloses a preparation method of a three-dimensional umbilical cord mesenchymal stem cell exosome and application of the three-dimensional umbilical cord mesenchymal stem cell exosome in the fields of medicines and medical beauty. The preparation method comprises the step of amplifying umbilical cord mesenchymal stem cells (UC-MSCs) by adopting a three-dimensional culture system. Experiments show that IGF-1, EGF, VEGF, TGF-beta, PDGF, FGF and the like in the exosome subjected to three-dimensional culture are beneficial to skin repair and oxidation resistance, and the content of cell active factors for promoting collagen secretion is remarkably increased. The umbilical cord mesenchymal stem cell exosome significantly improves the fibroblast proliferation activity, and UV-induced skin fibroblast damage model construction and function verification experiments also prove that the exosome can promote the HA level recovery of damaged skin fibroblasts. The invention provides an efficient and safe biological material for tissue repair.

The invention discloses a method for enhancing mechanical force of CAR-T cells on target cells to overcome mechanical immune checkpoints of SFs and application, and relates to the technical field of crossing of immunotherapy and biomechanics, in particular to CAR-T cells targeting fibroblast activated protein. The invention discloses a CAR-T (chimeric antigen receptor T-T) cell and SFs (small-form-factor s) DNA (deoxyribonucleic acid) nano connector for connecting the CAR-T cell with the SFs, the formation of a CAR cluster and mechanical force transmission are promoted by shortening the membrane spacing of an immune synaptic interface to 16-28nm, the DNA nano connector is formed by self-assembling complementary tetrahedral DNA structures, and the TDS comprises a single-stranded DNA vertex S4 with the length of 24-58 nucleotides. According to the method for enhancing the mechanical force of the CAR-T cells on the target cells to overcome the mechanical immune checkpoints of the SFs and the application, the escape and killing problems of the soft target cells are solved, the soft target cells are efficiently removed, and the killing rate of the CAR-T cells treated by DNJ-17 on the SFs is remarkably increased.

The present invention relates to a method for producing a skin tissue model, comprising: (a) isolating and culturing fibroblasts and keratinocytes from skin tissue; (b) preparing a bioink composition for a dermal layer and a bioink composition for an epidermal layer, the bioink composition containing the isolated cells; and (c) producing a skin tissue model using the bioink composition for a dermal layer and the bioink composition for an epidermal layer.

The present invention discloses an in vitro preparation method of human mesenchymal stem cells. The method comprises the following steps: using MSC culture medium supplemented with chemical small molecules to induce iPSC-derived MSCs; after the iPSC cell confluence reaches 40%, the culture medium containing chemical small molecules is replaced; after 8-10 days of induction, the cells are collected and passaged; and the cells are cultured to the P1 generation, showing uniform fibroblast-like, spindle-shaped or fusiform cells. The prepared hiPSC-MSCs meet the relevant phenotypes of mesenchymal stem cells, wherein the positive rates of CD73, CD90 and CD105 are higher than 95%, and the cells have immunomodulatory activity.

The invention relates to an estrogen receptor alpha and fibroblast activating protein dual-targeting near-infrared fluorescent probe as well as a preparation method and medical application thereof. Specifically, the probe can be used as a fluorescence probe for developing cells co-expressing the estrogen receptor alpha and the fibroblast activating protein, and can also be used as a near-infrared fluorescence imaging probe for diagnosing benign / malignant lesions such as nodules / tumors co-expressing the estrogen receptor alpha and the fibroblast activating protein. The fluorescent probe can also be applied to research on related biological events in cells for co-expressing the estrogen receptor alpha and the fibroblast activating protein, and particularly can be applied to fluorescent navigation in tumor resection operations for co-expressing the estrogen receptor alpha and the fibroblast activating protein; particularly, the composition can be applied to the synergistic treatment of an anti-estrogen therapy and a photodynamic therapy on a diseased region which co-expresses the estrogen receptor alpha and the fibroblast activating protein.

The present invention refers to molecules targeting membrane associated latent-transforming growth factor beta-binding protein 2 (LTBP2). Further the invention relates to molecules targeting LTBP2 for treatment and diagnosis of diseases associated with LTBP2 expressing fibroblasts. In particular, the invention refers to a nucleic acid encoding molecule targeting LTBP2 and a cell expressing the molecule targeting LTBP2.

The invention discloses a method for inducing fibroblasts to be reprogrammed into mesenchymal matrix-like cells (MLCs) by using small chemical molecules and application of the mesenchymal matrix-like cells (MLCs) in the anti-aging field. The invention discloses a set of specific chemical small molecule composition and an induction scheme thereof, which can be used for efficiently reprogramming fibroblasts from somatic cells into MLCs. Furthermore, the huge potential of the MLCs obtained by the method in anti-aging research is deeply explored, and the effect of the MLCs is proved to be obviously superior to that of umbilical cord mesenchymal stem cells in the aspects of reversing cell aging phenotypes and recovering tissue functions. The invention not only provides a new stem cell source strategy for avoiding the ethical and safety problems of the traditional reprogramming technology, but also lays a solid cell foundation for developing a novel, safe and effective anti-aging therapy based on cell transplantation or secreted factors.

The invention provides a gene editing cell and a method for producing extremely high molecular weight hyaluronic acid by using the cell. The preparation method comprises the following steps: by taking PUC57 as a skeleton carrier, constructing a recombinant plasmid containing an HAS2 gene, and transfecting a mole NMRHas2 gene, a chicken CHICKHAS2 gene, an astronomy mouse SNMHAS2 gene, a dakalimeris mouse DMRHAS2 gene or a mole mouse BMRHAS2 gene into chicken fibroblasts through lipidosome to construct a stably transfected cell line, so that hyaluronic acid with extremely high molecular weight can be stably obtained. The method provided by the invention overcomes the defects of non-uniform molecular weight distribution of HA product sources, low molecular weight and single method in the prior art, and has important significance for developing medicines for treating osteoarthritis and novel medical equipment products.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with functions of resisting aging and whitening skin and application of the compound active peptide. Comprising acetyl hexapeptide-1, nonapeptide-1, acetyl tetrapeptide-11, hexapeptide-9 and palmitoyl hexapeptide-12. The compound peptide disclosed by the invention can play a synergistic anti-aging and whitening role on an epidermal layer and a corium layer, and has multiple advantages; the compound peptide acts on epidermal layer keratinocytes, dermal layer fibroblasts and B16F10 cells, and can be used for detecting the senescence of the cells by influencing the expression quantity of cell senescence marker genes, influencing the cell cycle, influencing the expression quantity of extracellular matrix related proteins, influencing melanin synthesis and influencing the secretion quantity of acetylcholine in the cells. Multiple skin anti-aging and whitening effects synergistically exerted on the epidermal layer and the corium layer are achieved.

The present application relates to a kind of human gastric cancer primary fibroblast separation culture and purification method and its application.The human gastric cancer primary fibroblast separation culture and purification method provided by the present application are simple, efficient.The separation culture method and the purification method provided by the present application can complete tissue dissociation in shorter time, and cooperate multiple immunomagnetic beads to remove miscellaneous cells, so that the gastric cancer primary fibroblast group of stable, relatively consistent morphology adherent growth is obtained, phenotype observation is facilitated, and the interference of miscellaneous cells to subsequent function experiment is reduced.Enriched cells are expanded after culture, and can be used for the subtype research of fibroblast, microtumor environment related function experiment.

Methods and compositions relating to the treatment of degenerative disc disease are provided.SOLUTION: In some embodiments, cannabidiol is utilized to enhance the therapeutic and / or regenerative activity of fibroblasts. In other embodiments, cannabidiol is utilized to augment the reparative effects of other regenerative cells, including monocytes, mesenchymal stem cells, and / or hematopoietic stem cells. In some embodiments, cannabidiol is utilized in the culture media of regenerative cells prior to administration of said cells.SELECTED DRAWING: Figure 1

The application discloses application of miR-30a-5p in preparation of a product for treating airway stenosis. The application finds that miR-30a-5p plays an important regulatory role between airway basal stem cells and airway fibroblasts, and through the carrying of extracellular vesicles, miR-30a-5p is transmitted from the airway basal stem cells to the airway fibroblasts, targets and regulates the expression of a key gene FAP, inhibits the activation of the airway fibroblasts, reduces the collagen gel contraction ability of the airway fibroblasts, improves the extracellular matrix remodeling of the airway fibroblasts, can effectively stabilize the tracheal granulation tissue, prevents the recurrence of airway stenosis, and has application value for treating airway stenosis.

The invention discloses a peptide with an anti-photoaging effect as well as a preparation method and application of the peptide. The cyclic dipeptide cyclo (LO) and the linear dipeptide LO disclosed by the invention can be used for remarkably inhibiting the activity of MMP-1 and efficiently blocking collagen degradation. In addition, the cyclic dipeptide and the linear dipeptide have no cytotoxic effect when being co-cultured with fibroblasts, so that the cyclic dipeptide and the linear dipeptide have no stimulation and side effects on cells, and are high in safety and good in stability. The cyclic dipeptide and the linear dipeptide disclosed by the invention can resist UVA (Ultraviolet A) damage, have a promoting effect on cell proliferation activity after UVA irradiation and have an anti-aging effect on cells after UVA irradiation. The two peptides disclosed by the invention can be used for preparing medicines or cosmetics for resisting light aging, resisting aging, beautifying and protecting skin. The peptide fragment disclosed by the invention has a good application prospect in the fields of medicines, cosmetics and the like.

The subject invention pertains to a novel method for treating Peripheral Arterial Disease (PAD) by leveraging the angiogenic potential oftype 2 cytokines IL-4 and IL-13 to enhance the efficacy of induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) and induced endothelial cells derived from fibroblasts (iECs). This present invention aims at enhancing the muscle regeneration and revascularization for obese and diabetes individuals.

The present invention is directed to a method for identifying i) metastatic potential of a cancer cell sample from a non-metastatic cancer or ii) potential for malignant alterations in a healthy cell, the method comprising the steps of: a) culturing a cancer cell sample from a non- metastatic cancer or a healthy cell sample in a composition comprising cancer-associated fibroblasts, CAFs, preferably in two-dimensional or three-dimensional cell culturing conditions; and b) monitoring morphological alterations in the sample cells during and / or after the culturing step a), and / or determining the expression level of biomarkers relating to metastasis in the sample cells during and / or after the culturing step a); wherein the sample cells which show morphological alterations relating to metastasis and / or changes in the expression levels of said biomarkers in comparison to the levels of said biomarkers measured from corresponding samples cultured in a composition comprising normal fibroblasts, NFs, are identified as having metastatic potential or potential for malignant alterations. The present invention is also directed to a system for culturing eukaryotic cells comprising a solid surface comprising at least one partition suitable for culturing eukaryotic cells, wherein said at least one partition contains a combined cell culture comprising i) cancer cells from a non- metastatic cancer or healthy cells and ii) cancer-associated fibroblasts, CAFs, wherein said partition is a well or chamber.

This invention discloses the application of the Six2 gene in promoting the reprogramming of fibroblasts into muscle stem cells, belonging to the field of biotechnology. The nucleotide sequence of the Six2 gene is shown in SEQ ID NO.1, and the fibroblasts are chicken fibroblasts. This invention uses chicken embryonic fibroblasts as the reprogramming target, and induces the reprogramming of chicken embryonic fibroblasts into muscle stem cells by overexpressing the Six2 gene, promoting the expression of stem cell-related genes MyoD, MyoG, Myf5, and Pax7. The circ_TCONS / gga-miR181-5p / Six2 regulatory network participates in the reprogramming process; it improves the efficiency of somatic cell reprogramming into stem cells, greatly promotes the application of reprogramming, and provides a new approach for the sustainable acquisition of muscle stem cells.

Described herein are methods, compositions, and kits for isolation of mitochondria from cryopreserved primary cells (e.g., fibroblast cells, mesenchymal stromal / stem cells) to be used in mitochondrial organelle transplantation (MOT) methods and compositions.

The present invention relates to the field of nerve repair technology, and discloses a hydrogen and magnesium ion pump catheter with a Janus structure for peripheral nerve defect repair and a preparation method thereof. The pump catheter is composed of a hydrophilic inner layer, a hydrophobic outer layer, and a reservoir middle layer. The hydrophilic inner layer and the hydrophobic outer layer are made of degradable polymer materials, and the reservoir middle layer is made of a mixture of one or more of polylactic acid, polyglycolide, or polylactic-glycolic acid and a magnesium-based material. The pump catheter has a Janus structure, the hydrophilic inner layer provides biophysical clues, improves the cell compatibility of the catheter, and promotes the adhesion and migration of nerve cells. The hydrophobic outer layer blocks the adhesion and invasion of unfavorable cells such as fibroblasts to the damaged area. The magnesium ion and hydrogen reservoir middle layer can continuously release hydrogen and magnesium ions, exerting biological effects, improving the nerve regeneration microenvironment, and improving the effect of the stent in repairing nerve damage.

The invention provides a fibroblast storage device and method, and relates to the technical field of microbiological devices.The device comprises a monitoring module, a control module and a storage module, the storage prediction module performs cell stage division according to the storage label and the real-time cell state data, establishes stage constraints, performs cell storage environment prediction by using the stage constraints and the real-time cell state data, and establishes a target prediction result; the environment detection module performs real-time acquisition of a storage environment of fibroblasts and establishes a storage environment data set; and the intelligent adjustment module takes the storage environment data set as an environment starting point, takes the target prediction result as a following target, executes search optimization of environment control under the constraint of the adjustment granularity identifier, and performs intelligent adjustment of the storage environment according to a search optimization result. The technical problem that the fibroblast storage reliability is poor in the prior art can be solved, and the technical effect of improving the storage reliability is achieved.

The invention provides FAP CAR mRNA, a polymer lipid nanoparticle delivery system and a preparation method and application thereof, and belongs to the technical field of biological medicine. The FAP CAR mRNA polymer lipid nanoparticle delivery system disclosed by the invention comprises FAP CAR mRNA, an RP182 polypeptide and a lipid nanoparticle. The FAP CAR mRNA polymer lipid nanoparticle delivery system which is uniform in particle size and good in stability is successfully prepared, the mRNA encapsulation efficiency is high, mouse RAW264.7 cells can be efficiently transfected, FAP CAR is expressed by macrophages, and the transfection efficiency is remarkably higher than that of the FAP CAR mRNA or other control vectors independently used. The macrophages (CAR-M cells) transfected by the FAP CAR mRNA delivered by the LNP can specifically recognize and kill the activated fibroblasts, and the proliferation of the fibroblasts is remarkably inhibited.

The invention relates to the field of cell culture. The invention particularly relates to culturing cells, such as fibroblasts, to obtain collagen sheets. Modified scaffolds have been found to improve the characteristics of the method. The invention also relates to a collagen sheet and to leather obtained by tanning such a collagen sheet.

The present invention belongs to the technical field of cosmetics, and relates to an organosilicon compound derived from ascorbic acid or retinoic acid, and a preparation method therefor and the use thereof. The ascorbic-acid-derived organosilicon compound prepared from ascorbic acid in the present invention shows higher cell proliferation-promoting activity on human immortalized keratinocyte cell line HaCaT and better skin anti-aging activity while maintaining anti-oxidant activity, and has good application potential in improving skin condition. Compared with retinoic acid, retinyl retinoate and pinacolone retinoate, the retinoic-acid-derived organosilicon compound prepared from retinoic acid has better proliferation-promoting activity on fibroblasts and human immortalized keratinocyte cell line HaCaT, has good light / thermal stability, potentially better transdermal absorption and causes less skin irritation, and has great development prospects.

The invention belongs to the technical field of biology, and discloses a serum-free culture medium and application thereof in obtaining nerve cells through embryo fibroblast transdifferentiation. The serum-free culture medium consists of a basic culture medium, a protein additive and cell culture auxiliary components, the protein additive consists of transferrin, a leukemia inhibition factor and a basic fibroblast growth factor; the final concentration of the transferrin is 5 ng / mL-30 ng / mL, the final concentration of the leukemia inhibition factor is 50 ng / mL-150 ng / mL, and the final concentration of the basic fibroblast growth factor is 10 ng / mL-25 ng / mL. The serum-free culture medium can promote the conversion speed of transdifferentiation of fibroblasts into nerve cells, can significantly improve the transdifferentiation efficiency, and can improve the cell survival rate of transdifferentiation of mouse embryo fibroblasts into nerve cells.

The invention relates to the technical field of biomedicine, in particular to a method for identifying fibroblast subsets in colorectal cancer tissue based on single cell sequencing, and provides a method for identifying fibroblast subsets in colorectal cancer tissue based on single cell sequencing. The method comprises the following steps: S1, extracting colorectal cancer tissues: obtaining tumor tissues removed from the colorectal cancer tissues, and collecting a suspension to prepare a single-cell suspension; s2, single cell sequencing analysis: constructing a high-throughput sequencing library; s3, data analysis: processing the sequencing data by using a CellRanger software pipeline, and mapping readings to a genome and a transcriptome; and carrying out data dimension reduction and visualization. S4, identification of the tumor-associated fibroblast subpopulation: after the total cell type in the colorectal cancer tumor tissue is identified, annotating the cells; and identifying differentially expressed genes by using a Seurt software package, and carrying out function enrichment analysis, so as to identify various cell types.

The preparation method comprises the following steps: firstly, screening out No.3 polypeptide with the most obvious tumor cell migration promoting effect from supernatant proteins of cancer-related fibroblast (CAF) cells of primary tumor lesions and metastatic tumor lesions of an ovarian cancer patient, and naming the No.3 polypeptide as POCMM1; scratch experiments confirm that the POCMM1 promotes migration of ovarian cancer cells and has concentration dependence, and further prove that the matrix factor POCMM1 promotes development of tumors and has the potential of serving as a biomarker for ovarian cancer diagnosis. The expression level of the matrix factor POCMM1 in serum of an ovarian cyst patient, an ovarian cancer patient without metastasis and an ovarian cancer patient with metastasis is subsequently detected, and compared with the expression level of the matrix factor POCMM1 in the serum of the ovarian cyst patient, the expression level of the matrix factor POCMM1 in the serum of the ovarian cancer patient is increased; the expression in serum of a metastatic ovarian cancer patient is the highest, so that the matrix factor POCMM1 can be confirmed to be used as a biomarker for ovarian cancer diagnosis.

The application relates to the technical field of biomaterials, and particularly discloses a PHA combined microsphere as well as a preparation method and application thereof. The PHA combined microsphere provided by the application contains 10-40 wt% of PHA microspheres with a weight average molecular weight of less than 30000 Da and 1-40 wt% of PHA microspheres with a weight average molecular weight of more than 100000 Da, with the PHA being selected from one or more of poly-beta-hydroxybutyric acid, a copolymer of 3-hydroxybutyric acid ester and 3-hydroxyvaleric acid ester, a copolyester of 3-hydroxybutyric acid and 3-hydroxyhexanoic acid and poly(3-hydroxybutyric acid-co-4-hydroxybutyric acid). After the PHA combined microsphere is filled in the skin, on one hand, the PHA combined microsphere can be rapidly degraded in an early stage and promote the reproduction of fibroblasts and endothelial cells in a short time; on the other hand, the PHA combined microsphere can be continuously degraded within 21 weeks and cannot cause inflammation, so that the effect of persistent filling is achieved.