283 results about "Fibroblastic cell" patented technology

The invention discloses a bionic vascularized soft tissue with a multilayer vascular structure and a preparation method thereof. The preparation method comprises the steps of: employing a moulding process or 3D printing way to obtain a sacrificial membrane with a branched structure; coating or spraying a polymer solution containing a pore-foaming agent on the surface of the sacrificial membrane toobtain a polymer film; removing the treated sacrificial membrane of the structure and the pore-foaming agent to obtain a vascular channel-like structure with surface pores; conducting perfusion planting of endothelial cells in the channel of the vascular channel-like structure; planting smooth muscle cells or fibroblasts in the surface pores of the vascular channel-like structure; and placing thetreated vascular channel-like structure in a mold, adding an aquogel solution containing parenchymal cells, and conducting moulding so as to obtain the bionic vascularized soft tissue. The method provided by the invention can construct bionic blood vessels with a multilayer structure in artificial tissues.

The invention discloses an inducing culture medium for inducing fibroblast to trans-differentiate into cardiac muscle cells, a method and an application of the inducing culture medium. The inducing culture medium comprises a basic culture medium and an inducing small molecular assembly which is 6TCFOW or SCFOV, wherein 6 is E61541, T is tranylcypromine, C is CHIR99021, F is forskolin, O is Dorsomorphin, W is IWR-I, S is SB431542, and V is valproic acid. The inducing culture medium can trans-differentiate the fibroblast into the cardiac muscle cells which have normal cardiac muscle cell specific molecular tags and a normal cardiac muscle function, so that a new way is provided for solving the cell source problem of the regenerative medicine.

The invention relates to a method for culturing seed cells of tissue engineering. The method for amplifying the seed cells of the tissue engineering comprises the following steps of: (1) adding a macroporous microcarrier into a bioreactor, hydrating and sterilizing and then adding 50 to 100ml of fibroblast culture medium, and adding fibroblasts for culturing; (2) inhibiting the fibroblasts from amplifying when the fibroblasts are attached to the microcarrier and the growth area is near 50 percent of surface area of the microcarrier, and standing and then pouring out culture solution; and (3) washing the obtained carrier by using sterile, calcium-free and magnesium-free phosphate buffered solution (PBS), then adding an epidermal cell culture medium and epidermal cells, and continuing to stir and culture until the cells overgrow the carrier to obtain the seed cells of skin tissue engineering. The quantity and function of the cells cultured by the method can both be apparently increased,the damage of the cells is reduced, the cells can be directly used as seed cells for skin construction of the tissue engineering, and the effect is superior to those of methods using epidermal cell suspension and epidermal cell patches.

The present invention provides one kind of double-layered artificial skin implant comprising one hypodermal layer containing polyglycollic acid and other biodegradable material, fibroblast and matrix protein; and one epidermal layer on the hypodermal layer. The artificial skin has continuous epidermal cell growing on the surface of the fibroblast-PGA composite, well cell differentiation and obvious lamination. The slice shows the artificial skin has the tissue structure characteristic similar to that of normal body skin. The preparation process and use of the artificial skin is also provided.

The invention discloses an induction medium inducing transdifferentiation of fibroblast into adipocyte, an induction method and application thereof. The induction medium contains a basal culture medium and an induction micromolecule combination, wherein the induction micromolecule combination is SG or 6TF, S is SB431542, G is GSK126, 6 is E61541, T is tranylcypromine, and F is forskolin. The induction medium can induce and trans-differentiate the fibrolast into the adipocyte, the obtained adipocyte contains a normal adipocyte specificity molecular tag and has a normal fat adipogenesis function, and a new approach is provided for solving the cellular source problem of regenerative medicine.

A transcutaneous prosthesis includes a first component shaped for implantation into a bone, the first component including flutes or grooves on a surface thereof for deterring rotation of the prosthesis within a bone; a second component adapted for location between the bone and the skin, the second component having a surface treatment for stimulation of fibroblastic cell proliferation and attachment of epithelial cells; and a third component adapted for location to extend from the skin surface and is adapted to extend directly from the skin surface in use, the third component having a coating of a non-stick material on an outer surface thereof, the coating having a surface energy that is lower than a surface energy of the first and second components and which is low enough to deter bacterial adhesion.

The present invention provides methods, compounds and kits for increasing the viability of cells. The methods involve treating cells that make up a tissue graft with erythropoietin before, during or after delivery or administration. The method can employ cells of different types, including cells of neural or paraneural origin, such as adrenal chromaffin cells. Also useful are cell lines grown in vitro. Cells not of neural or paraneural origin, such as fibroblasts, may also be used following genetic alteration to express a desired neural product such as a neurotransmitter or a neuronal growth factor. The method is used to treat neurological diseases such as Parkinson's disease, Alzheimer's disease, Huntington's disease, epilepsy, and traumatic brain or spinal cord injury.

The invention discloses four cell types which have wide heteroplastic transplantation application prospect and can be effectively induced into induced pluripotent stem (iPS) cells. The origins of three cell types are placental tissue, namely amnion mesenchymal cell, chorion mesenchymal cell and umbilical cord mesenchymal cell; and the origin of the other one is amniotic fluid cell. Besides, the invention also discloses an induction reprogramming method for producing cells capable of producing induced pluripotent stem (iPS) cells by efficient induction, including the following steps: cDNA containing pluripotent stem cell factor is respectively introduced into the four primary culture cells; the four primary cells in which cDNA is introduced are respectively cultured on appropriate culture mediums, primary iPS is obtained and then quality of iPS is optimized on appropriate culture mediums, and cloning of pluripotent stem cell can be primarily evaluated. In the invention, three mesenchymal cells of placenta origin and amniotic fluid cell all can be induced into iPS, wherein the chorion mesenchymal cell is compared with fibroblast, efficiency of induction reprogramming is improved by over 100 times, efficiency is about 2.3%, and the efficiency is slightly higher than that of horny cell; and a means which is more effective and more pertinent is provided for building disease model, screening drug and controlling oriented differentiation.

Compositions for use in treatment of a variety of tissue diseases include stem cells and stem cell released molecules (SRM's) suspended in an aqueous solution with a cellulosic material or other thickening agent. The stem cells and SRM's can be derived from one or more distinct cell lines. The SRM's can further include one or more mucins, cytokines, or growth factors. Exemplary formulations include stem cells and SRMs derived from epithelial stem cells, corneal limbal stem cells, and fibroblasts. Other compositions and methods for formulation thereof are described.

The invention provides a method for building a three-dimensional skin model. The method comprises the following steps: 1) preparing an extracellular matrix polymeric hydrogel solution; 2) mixing fibroblast and the extracellular matrix polymeric hydrogel solution, and culturing through a small culture chamber; and 3) inoculating cutin to form cells; performing air-liquid interface culture to obtain the three-dimensional skin model containing a corium layer, an epidermal layer and a cutin layer. The method is capable of effectively building the three-dimensional skin model; in addition, the preparation method is simple and convenient, and can be applied to the sieving of anti-inflammatory and anti-allergy skin drugs; and the method has a good application prospect.

The invention relates to application and a preparation method of an autologous fibroblast-sourced freeze-dried exosome powder. The autologous fibroblast-sourced freeze-dried exosome powder is appliedinto wrinkle resistance and oxidation resistance, and the application method is as follows: normal saline is used for dissolving the autologous fibroblast-sourced freeze-dried exosome powder into 0.5percent by weight of solvent, so that injection is obtained, and the injection is injected deep into deep stratum of the dermis by a microneedle. The preparation method of the freeze-dried exosome powder comprises four steps, i.e., obtainingment of skin-sourced fibroblasts, cell amplification and cell identification, separation and identification of exosome and freeze-drying of exosome. Since theinvention adopts the autologously sourced exosome to resist wrinkles and oxidation, the autologous fibroblast-sourced freeze-dried exosome powder is easy to store and transport, ethical problems do not exist, moreover, safety is high, and the wrinkle-resistant and oxidation-resistant effect is good.

The present invention belongs to the field of tissue engineering and medical material technology, and is especially titanium net with fimbrin coating and its preparation process and application in reconstructing trachea default. The present invention prepares fimbrin-titanium net complex with medical titanium net as support material and fimbrin to form coating. Fimbrin has excellent biocompatibility and can promote the growth of fibroblast and mucosa cell, speed healing, reduce complications. Clinically, the present invention is used in repairing trachea default. The present invention is significant in the research of fimbrin in promoting repair of trachea mucosa, reconstructing trachea default and reducing complications during reconstructing trachea default.

The invention discloses an asymmetric double-crosslinked composite material, as well as a preparation method and the application of the material. The asymmetric double-crosslinked composite material is obtained by preparing sodium hyaluronate/chitosan composite material into an asymmetric surface material with a smooth surface and a spongy surface, has good hydrophilicity, water absorbency, water storability and breathability, has the functions of promoting growth of epithelial cells and endothelial cells and inhibiting growth of fibroblasts, has a wide antibacterial effect, and has a wide application prospect in the field of medical care.

The invention relates to an injectable tissue patching material, and the material is characterized in that after injected to a recipient area, the mixture of viable cells, drug control releasing microspheres and gel can be shaped rapidly, filling up the impairment of soft tissues and promoting damage healing. Compared with the prior art, the injectable tissue patching material prepared in the invention has the advantages of high biocompatibility, rapid and free shaping and filling up the impairment of organs and tissues rapidly. The material can promote growth of fibrous cells, formation of neoformative blood capillaries, generation of granulation tissues and damage healing. The contained growth factors that are released continuously for a long time or drugs that promote healing can promote in vivo cells or implanted cells to produce bigger restoration function.