Application and preparation method of autologous fibroblast-sourced freeze-dried exosome powder

Abstract

The invention relates to application and a preparation method of an autologous fibroblast-sourced freeze-dried exosome powder. The autologous fibroblast-sourced freeze-dried exosome powder is appliedinto wrinkle resistance and oxidation resistance, and the application method is as follows: normal saline is used for dissolving the autologous fibroblast-sourced freeze-dried exosome powder into 0.5percent by weight of solvent, so that injection is obtained, and the injection is injected deep into deep stratum of the dermis by a microneedle. The preparation method of the freeze-dried exosome powder comprises four steps, i.e., obtainingment of skin-sourced fibroblasts, cell amplification and cell identification, separation and identification of exosome and freeze-drying of exosome. Since theinvention adopts the autologously sourced exosome to resist wrinkles and oxidation, the autologous fibroblast-sourced freeze-dried exosome powder is easy to store and transport, ethical problems do not exist, moreover, safety is high, and the wrinkle-resistant and oxidation-resistant effect is good.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to an application and preparation method of exosome freeze-dried powder derived from autologous fibroblasts. Background technique [0002] Wrinkles are due to the lack of collagen, hyaluronic acid, elastic fibers and other substances in the dermis of the skin, which cannot support the normal structure of the skin, and the epidermis of the skin collapses to form gullies. These important substances are all secreted by fibroblasts. That is to say, fibroblasts are the main effector cells in the process of wrinkle formation. Collagen makes up 70-80% of the dry weight of the skin and contributes to the stability and structural integrity of the tissue. A large number of studies have proved that the gradual decline of collagen synthesis in the dermis leads to the formation of wrinkles and aging skin, collagen directly affects skin tension, and the maintenance of collagen structure is relate...

AI Technical Summary

Problems solved by technology

However, at present, in the repair of skin wounds, stem cells mainly rely on paracrine to promote the repair of skin wounds, and the transplantation of stem cells to the injured site has a low differentiation rate and a short-term effect.

In addition, the use of stem cells has limitations such as difficulties in storage and transportation, ethics, tumorigenic mutations, and immune rejection. Therefore, it is very important to find a method that has the curative effect of stem cells and can overcome the shortcomings of stem cell therapy.

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