Multilayer core-shell nano-fiber scaffold, and method for constructing tissue engineering material by using multilayer core-shell nano-fiber scaffold and melanocyte

A multi-layer core-shell structure and nanofiber technology, applied in the field of biomedicine, to achieve good mechanical properties, good shape, and improve material strength

Active Publication Date: 2016-04-27
HANGZHOU THIRD HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The core-shell nanofiber scaffolds disclosed above cannot achieve long-term sustained release of drugs, let alone be used for the cultivation of human melanocytes

Method used

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  • Multilayer core-shell nano-fiber scaffold, and method for constructing tissue engineering material by using multilayer core-shell nano-fiber scaffold and melanocyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1. Preparation of chitosan-polyethylene oxide-polyethylene oxide multilayer core-shell nanofiber scaffold material

[0053] A. chitosan is dissolved with trifluoroacetic acid / dichloromethane weight ratio 3:1 mixed solvent, is made into the shell solution that concentration is 5%;

[0054] B. Dissolve polyethylene oxide, polyethylene glycol methacrylate, and polyethylene glycol dimethacrylate in water at a weight ratio of 80:20:1 to form a middle layer solution with a concentration of 5%. Add 1% photoinitiator 1-[4-(2-hydroxyethoxy) phenyl]-2-hydroxyl-2methyl-1-propan-1-one before spinning;

[0055] C. polyethylene oxide is dissolved in water to make a concentration of 4% nuclear layer solution;

[0056] D. Electrospinning is carried out under the conditions of voltage of 15KV, three-layer propulsion rate of 0.5ml / h, receiving distance of 15cm, temperature of 30oC, and ultraviolet light irradiation;

[0057] E. drying the multi-layer core-shell nanofiber sup...

Embodiment 2

[0062] Example 2. Preparation of chitosan-polyvinylpyrrolidone-polylactic acid core-shell nanofiber scaffold material

[0063] A. Dissolve chitosan with acetic acid / water (weight ratio 1:1) mixed solvent to make a chitosan / polyethylene oxide solution with a concentration of 4%;

[0064] B. Dissolve vinylpyrrolidone, hydroxyethyl methacrylate, and N,N'-methylenebisacrylamide in water at a weight ratio of 70:30:3 to form a 6% nuclear layer solution. Add 3% photoinitiator phenyl bis(2,4,6-trimethylbenzoyl) phosphine oxide before spinning;

[0065] C. Polylactic acid is dissolved with dichloromethane to make a solution with a concentration of 7%;

[0066] D. Under the conditions of 20KV voltage, 0.5ml / h shell propulsion rate, 0.8, 0.4, 0.4ml / h shell, middle and core propulsion speeds, receiving distance 18cm, temperature 20oC, and ultraviolet light perform electrospinning;

[0067] E. the chitosan-polyvinylpyrrolidone-polylactic acid multilayer core-shell nanofiber scaffold was b...

Embodiment 3

[0072] Example 3. Preparation of gelatin-polyvinyl alcohol-polyvinylpyrrolidone core-shell nanofiber scaffold material

[0073] A. Dissolve the gelatin with trifluoroethanol / water (mass ratio 8:2) to make a 10% gelatin solution;

[0074] B. Dissolve polyvinyl alcohol, methacrylic acid, and ethylene glycol dimethacrylate in acetic acid / water (mass ratio 1:1) at a weight ratio of 60:40:0.4, and the concentration is 3%. The core layer solution, add 0.2% photoinitiator 2-hydroxyl-1-[4-(hydroxyl) phenyl]-2-methyl-1-propanone before spinning;

[0075] C. Electrospinning is carried out at a voltage of 25KV, a shell-layer propulsion rate of 1ml / h, a core-layer propulsion rate of 0.5ml / h, a receiving distance of 20cm, a temperature of 40oC, and ultraviolet light;

[0076] D. dissolving polyvinylpyrrolidone with ethanol to make a concentration of 8% nuclear layer solution;

[0077] E. Bake the gelatin-polyvinyl alcohol-polyvinylpyrrolidone multilayer core-shell nanofiber scaffold unde...

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Abstract

The invention discloses a multilayer core-shell structured nano-fiber scaffold, and a method for constructing a tissue engineering material by using the multilayer core-shell structured nano-fiber scaffold and melanocyte. The multilayer core-shell nano-fiber scaffold is prepared through electrostatic spinning, the external layer of the scaffold is a biocompatible material, the middle layer of the scaffold is a polymer barrier layer, and the core layer of the scaffold is a drug supported core material. The tissue engineering material can be formed by using the multilayer nano-fiber scaffold and the melanocyte, or using the multilayer nano-fiber scaffold, fibroblast and keratinocyte, and can be used to treat depigmentation (such as leucoderma). The multilayer core-shell structured nano-fiber scaffold has the advantages of good biocompatibility and mechanical performances, and realization of long-time controllable release of drugs, and the tissue engineering scaffold constructed by using the multilayer core-shell structured nano-fiber scaffold can effectively support growth, propagation and transplantation of melanocyte and co-culture cells.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a multi-layer core-shell nanofiber scaffold with drug sustained release function and a method for constructing tissue engineering materials with melanocytes. Background of the invention [0002] Vitiligo is a common multiple depigmentation skin disease. The clinical manifestations are mainly localized or localized white patches with clear boundaries and no symptoms. Histology and immunocytochemistry show that the epidermal melanocytes in the lesions disappear. . Vitiligo has an incidence rate of 0.5-2% in the population. Due to the lack of melanin in the leukoplakia, it is easy to be burned by the sun and even cause cancer. At the same time, because it affects the appearance of the human body, it often causes serious psychological obstacles to patients and reduces their quality of life. Traditional treatment methods mainly include drug therapy and light therapy. But drug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F8/18D01F8/16D01F8/10D01F8/14D01F8/02D06M11/56D06M11/76D06M11/38D06M13/224D06M13/123D04H1/4382D04H1/728A61L27/16A61L27/20A61L27/22A61L27/24A61L27/38A61L27/50A61L27/54A61L27/60A61L27/18D06M101/10D06M101/30D06M101/32D06M101/26D06M101/18
CPCA61L27/16A61L27/18A61L27/20A61L27/222A61L27/225A61L27/24A61L27/38A61L27/3804A61L27/3813A61L27/50A61L27/54A61L27/60A61L2400/18D01F8/02D01F8/10D01F8/14D01F8/16D01F8/18D04H1/4382D04H1/728D06M11/38D06M11/56D06M11/76D06M13/123D06M13/224D06M2101/10D06M2101/18D06M2101/26D06M2101/30D06M2101/32C08L5/08C08L29/14C08L33/08C08L29/04C08L33/10C08L71/02
Inventor 许爱娥王文俊吴非凡尉晓冬
Owner HANGZHOU THIRD HOSPITAL
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