46 results about "Nanofiber scaffold" patented technology

The invention discloses an integral forming method of a myocardial chip embedded with nanofibers and a flexible circuit board, which adopts integral forming equipment, integrates a photocuring part and an electrostatic spinning part, and realizes collaborative operation through an upper computer and a driving device; the method comprises the following steps: firstly, forming a lower runner layer by adopting a hydrogel material through a photocuring component, and embedding a flexible circuit board into the top of the lower runner layer; forming a nanofiber bracket on the flexible circuit board by adopting a spinning material through the electrostatic spinning part; a hydrogel material is printed on the nanofiber support to form an upper flow channel layer, flow channel packaging is achieved, and efficient transmission of electrical stimulation and field potential signals is achieved through a flexible circuit board. According to the method, the problems of low alignment precision, strong manual dependence and insufficient automation degree in the traditional process are solved, the chip manufacturing efficiency and consistency are improved, and a detection platform with high integration level and high biocompatibility is provided for drug screening and heart disease research.

According to the invention, a bicontinuous phase conductive core-shell nanofiber membrane is applied to construction of a flexible neural electrode and a neural conduit, firstly, a bicontinuous phase conductive polymer system is constructed, and the system realizes a three-dimensional network in which an electric phase and a mechanical phase are respectively continuous, interpenetrating but independent; and the long-term technical problems of conductive component agglomeration, conductive path interruption and the like are effectively solved. And preparing the conductive nanofiber scaffold with a core-shell structure by taking a high-molecular polymer as a core layer material and a bicontinuous-phase conductive polymer system as a shell layer material through a coaxial electrostatic spinning method. The construction mode effectively establishes a stable, continuous and uniform electron conduction path, can realize effective nerve electrical stimulation and high-fidelity electrophysiological signal recording in vivo, and can be used as a nerve conduit to regulate and repair related bio-electricity signals in a nerve repair process, so as to accelerate regeneration and repair of nerves.

The invention discloses a device and method for preparing a three-dimensional nanofiber scaffold by using flowing liquid, and relates to the technical field of wet electrostatic spinning, and the device comprises an injector, a high-voltage power supply, a receiving pool A, a metal layer, a flow guide pipe, a receiving pool B, a metal screen, a replaceable filter, a flow rate control device and a water suction pump. A needle of the injector is connected with positive high voltage of the high-voltage power source, the injector is located above the receiving pool A, the receiving pool A is located above the receiving pool B, a round hole is formed in the center of the receiving pool A, and the receiving pool A is connected with the receiving pool B through a flow guide pipe. A metal layer is arranged at the bottom of the receiving pool A, and an electric field is formed between the metal layer and the needle of the injector. A replaceable filter is mounted at an outlet of the receiving tank B, a solution in the receiving tank B is introduced into the receiving tank A through a liquid flow pipeline and a water suction pump, and a flow speed control device controls the flow speed of the liquid. The device provided by the invention is used for preparing the three-dimensional nanofiber scaffold which has high porosity and simulates an ECM fiber structure.

A nanodiamond-reinforced nanofiber scaffold system for skin regeneration, consisting of a multilayered, electrospun nanofiber matrix formed from one or more biocompatible polymers selected from polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), chitosan, gelatin or collagen, wherein nanodiamonds are present in an amount of 0.1 to 5 wt.-% are incorporated and surface-functionalized with oxygen, hydroxyl, or carboxyl groups to ensure homogeneous dispersion within the polymer matrix, the scaffold being characterized by a controlled fiber diameter in the range of 100 to 800 nm, an interconnected porosity between 60 and 90%, a tensile strength of 2 to 10 MPa, a degradation time of 2 to 8 weeks, and the ability to improve cell adhesion, proliferation, and migration of fibroblasts and keratinocytes, while simultaneously exhibiting antioxidant activity, reduced formation of reactive oxygen species, and the optional incorporation of bioactive agents selected from growth factors, antioxidants, or antimicrobial compounds to accelerate wound healing and skin tissue regeneration.

The invention discloses a periodontal complex stent with a bionic structure and a preparation method. The preparation method comprises the following steps: step 1, preparing a polycaprolactone-polylactic acid nanofiber membrane by an electrostatic spinning method; 2, the polycaprolactone-polylactic acid nanofiber membrane obtained in the step 1 is coated with chitosan layer by layer, the fiber membrane is stacked, and after freeze drying, a polycaprolactone-polylactic acid nanofiber scaffold is obtained; 3, immersing the polycaprolactone-polylactic acid nanofiber scaffold obtained in the step 2 into a collagen solution, and carrying out self-assembly to form a composite scaffold; then immersing into a hydroxyapatite solution, and mineralizing to obtain the required composite scaffold; according to the invention, a directionally arranged PCL-PLA nanofiber scaffold is taken as a basis, the surface layer is coated with porous collagen, and after mineralization, a mineralized structure with gradient decreasing from a collagen layer in an external phase to a middle fiber layer is obtained; the obtained composite scaffold simulates a heterogeneous structure of periodontal membrane-bone interface tissue, and provides possibility for collaborative regeneration of soft and hard tissues of a periodontal complex.

The invention provides a shape memory nano short fiber stent with self-adaptive capacity and preparation and application thereof, and belongs to the technical field of tissue engineering materials. The preparation method comprises the following steps: firstly, preparing flexible polylactic acid-fullerol composite nanofibers containing uniformly dispersed fullerol nanocrystals by utilizing an electrospinning technology, then dividing the flexible polylactic acid-fullerol composite nanofibers into short fibers by virtue of homogenization and freeze-drying processes, and assembling the short fibers into a three-dimensional porous scaffold by taking chitosan as an adhesive. The porous scaffold material disclosed by the invention has excellent biological characteristics and a water-triggered shape memory effect, and due to the fact that chitosan has excellent antioxidant activity and inherent bone induction characteristics, the nanofiber scaffold with the controllable and recoverable shape developed by the invention is expected to effectively promote osteoporotic bone healing.

A method of activating a piezoelectric neuroconduit guide scaffold including a plurality of aligned piezoelectric polymer nanofibers, the method including mechanically stimulating the piezoelectric neuroconduit guide scaffold with hydro-acoustic waves or shockwaves to remotely activate a piezoelectric effect of the nanofibrous scaffolds that induces a mechano-electrical stimulus on neural cells cultured on the scaffold, wherein the mechano-electrical stimulus promotes nerve fiber outgrowth from the neuronal cells. Some aspects relate to seeding individual components of neural tissues on the piezoelectric neuroconduit guide scaffold, wherein the hydro-acoustic stimulation induces neural tissue formation. In other aspects, the piezoelectric neuroconduit guide scaffold is implanted in a damaged neural tissue, wherein stimulating the piezoelectric neuroconduit guide scaffold by the application of shockwaves promotes nerve fiber outgrowth that bridges a nerve gap to induce nerve regeneration or reinnervation of the damaged neural tissue.

The invention provides a silk nanofiber porous scaffold and a preparation method thereof, and belongs to the field of polymer nanofiber scaffold preparation.The preparation method comprises the steps that silk nanofibers and water are evenly mixed to obtain suspension liquid, then a cross-linking agent is added into the suspension liquid, the mixture is evenly stirred to obtain a mixed solution, and the mixed solution is dried to obtain the silk nanofiber porous scaffold. Placing the mixed solution in an environment of-20 DEG C to-4 DEG C for constant-temperature freezing treatment for 14-21 days so as to improve the efficiency of the cross-linking reaction and form a topological nanostructure in the scaffold by utilizing the freezing concentration effect of ice crystals on the nanofibers, and finally unfreezing to obtain the silk nanofiber porous scaffold with high porosity. The prepared silk nanofiber porous scaffold has good water stability, and the dissolution loss rate is lower than 20% when the silk nanofiber porous scaffold is soaked in deionized water at 37 DEG C for 24 h.

Provided herein are implantable biomaterials for promoting regeneration of an injured biological tissue, the biomaterials including piezoelectric materials and an extracellular matrix specific to the injured biological tissue, wherein the piezoelectric materials and the extracellular matrix are electrospun together to provide tissue-specific bioactive piezoelectric nanofiber scaffolds. Also provided herein are methods of fabricating a tissue-specific bioactive piezoelectric nanofiber scaffold and methods of promoting regeneration of injured biological tissue by implanting the disclosed bioactive piezoelectric scaffolds.

A triple antibiotic nanofiber scaffold for wound dressings, consisting of: (a) a polymer blend of polyacrylonitrile and pullulan in a weight ratio of about 10:1; (b) one or more fluoroquinolone active ingredients selected from levofloxacin, ciprofloxacin and ofloxacin in different concentrations; wherein the polymer-drug solution is electrospun using a voltage of about 15 kV and a flow rate of about 5 ml / h to form uniform nanofiber mats on a collector covered with aluminum foil, and where the nanofibers are configured to provide structural integrity, drug loading capacity, and potential wound healing properties.

The application discloses a self-powered electroactive dressing with a wound repair function and a preparation method and application thereof, and belongs to the technical field of medical devices. The electroactive dressing comprises a zinc-molybdenum primary battery structure and three layers of functionalized nanofiber scaffolds. The scaffold takes gelatin-polycaprolactone as a fiber matrix, sequentially loads zinc nanoparticles, icariin and molybdenum nanoparticles to form a functional layer, and is prepared through EDC / NHS cross-linking modification. The electroactive dressing has good mechanical properties and biocompatibility, can be minimally invasively implanted in a wound site, spontaneously generates a continuous and stable microelectric field through the zinc-molybdenum primary battery, and cooperatively realizes gradient-controlled release of icariin from burst release to sustained release, so that the effects of promoting fibroblast proliferation and migration and promoting rapid wound healing are achieved. The dressing has the advantages of simple preparation process, low cost, active regulation of a wound microenvironment, mild and controllable degradation, safety, no toxic side effects, and a very good application prospect.

The invention discloses a nanofiber scaffold and a preparation method thereof, and belongs to the field of medical materials. The nanofiber scaffold comprises a matrix and nanoparticles loaded on the surface of the matrix, raw materials of the matrix comprise TPU and EPO, raw materials of the nanoparticles comprise an iron source, PDA, tetrabutyl titanate and a barium source, and the size of the nanoparticles is 170-222nm. The preparation method of the nanofiber scaffold comprises the following steps: (1) preparing PDA / Fe-BTi nanoparticles through a hydrothermal reaction; (2) preparing a matrix comprising PEO (Polyethylene Oxide) and TPU (Thermoplastic Polyurethane); and (3) loading the nano-particles on the substrate by using an electrostatic spinning technology to obtain the nano-fiber scaffold. The nanofiber scaffold provided by the invention can achieve the effects of self-generation of current by virtue of an external magnetic field, excellent mechanical property and excellent conductivity.

The invention discloses a photo-thermal immunoregulation nanofiber scaffold and a preparation method and application thereof.The photo-thermal immunoregulation nanofiber comprises nanofiber with polylactic acid as a base material, and black phosphorus nanosheets and an HSP90 inhibitor 17-DMAG are jointly loaded in the nanofiber through a high-voltage electrostatic spinning technology; the photo-thermal immunoregulation nanofiber scaffold is applied to liver cancer postoperative immunotherapy through a photo-thermal therapy method. According to the photo-thermal immune regulation and control nanofiber scaffold, a PTT effect is locally generated under near-infrared irradiation, and a strong ISR-ICD cascade reaction is driven. Meanwhile, the exposed DAMPs enhance tumor immunogenicity, recruit cytotoxic T lymphocytes and reprogram an immunosuppressive cell population, so that an immunosilence microenvironment is remodeled into a responsive tumor microenvironment and cooperates with anti-PD-L1, lasting immune memory is established, and finally recurrence and metastasis of postoperative liver cancer are effectively inhibited.

The present invention relates to a biomaterial comprising: —a membrane wound patch (a), made of a nanofibrous polymeric scaffold, —a hydrogel (b) including autologous or allogenic bone marrow-derived mesenchymal stem cells, and —a bone wound patch (c) being a nanofibrous scaffold made of polymers, wherein said scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch (c) further comprises a bone growth factor; wherein the hydrogel (b) is included between the membrane wound patch (a) and the bone wound patch (c).

The invention discloses application of a composite biological material in hernia repair surgery, and belongs to the technical field of biotechnology, the composite biological material comprises a nanofiber scaffold, the scaffold is prepared by compounding polylactic acid-glycolic acid and silk fibroin, the weight ratio of the polylactic acid-glycolic acid to the silk fibroin is 70: 30, and the weight ratio of the polylactic acid-glycolic acid to the silk fibroin is 20: 30. The nanofiber scaffold is prepared through an electrostatic spinning technology and is used for providing mechanical support and promoting cell attachment and proliferation; the fiber is of a porous structure, the pore size is 10-100 microns, and cell permeation and nutrient substance exchange are facilitated; the polylactic acid-glycolic acid / silk fibroin composite scaffold is prepared by compounding polylactic acid-glycolic acid and silk fibroin, the mechanical properties of natural tissues are simulated, and a good growth scaffold is provided for cells. The gel layer is prepared through a solution phase separation method, and provides additional biological activity support and a cell attachment surface for the nanofiber scaffold. By arranging the antibacterial coating, the risk of postoperative infection is reduced, and meanwhile, protection is provided for materials and human tissues.

The present invention relates to a method for preparing a nanofibrous scaffold and its use for facilitating cell culture. In particular, the nanofibrous scaffold comprises cross-linked cellulose nanofibers that result in a continuous microcarrier material for use in industrial-scale cell culture devices.

The invention discloses a biological material for promoting optic nerve injury repair and a preparation method thereof.The biological material comprises a bionic nerve scaffold, a growth factor compound, an anti-inflammatory component and a light-operated neuroelectric signal guiding layer, the bionic nerve scaffold comprises a polycaprolactone-polyethylene glycol block copolymer and self-assembly neuropeptide, the growth factor compound comprises a neurosynaptic growth factor and a vascular endothelial growth factor, the anti-inflammatory component is liposome nanoparticles loaded with interleukin-10, and the light-operated neuroelectric signal guide layer is a polypyrrole film; the oriented nanofiber scaffold of polycaprolactone-polyethylene glycol and self-assembled neuropeptide is prepared by adopting a micro-fluidic spinning technology, a three-dimensional porous scaffold is formed to simulate a natural nerve bundle topological structure and guide oriented regeneration of axons, long-acting slow release is realized through gene activated growth factors, the axon extension efficiency is effectively improved, and the growth rate of the axons is increased. Through the interleukin-10 lipidosome anti-inflammatory component, the inflammation inhibition rate is increased, and secondary injury is relieved.

Nanofiber structures are provided as well as methods of use thereof and methods of making.

Oral cavity wound healing occurs in an environment that sustains ongoing physical trauma and is rich in bacteria. Patients undergoing cleft palate repair have a high degree of wound healing complications, such as oronasal fistula (ONF) formation. Following hard palate injury, ONF was created that demonstrated little change in pro-regenerative monocytes LY6Clo monocytes; however, there were increased M2 macrophages observed. Delivery of FTY720 nanofiber scaffolds following hard palate injury prevented ONF formation, allowed complete wound healing and was associated with increased LY6Clo monocytes and pro-regenerative M2 macrophages. Evaluation of interleukin gene expression revealed reduction in pro-inflammatory IL1 and IL6 and increased expression of pro-regenerative IL10 with FTY720 nanofiber delivery. The ability of FTY720 scaffolds to increase LY6Clo monocytes, increase M2 macrophages and alter the interleukin expression during hard palate mucosal healing demonstrates the ability of a FTY720-based autotherapy to improve oral cavity wound healing.

A composition including a plurality of non-covalently bonded peptide amphiphiles. The peptide amphiphiles include a hydrophobic alkyl tail moiety, a hydrophobic peptide portion including one or more hydrophobic amino acid residues, and a hydrophilic portion including one or more hydrophilic amino acid residues. Some or all of the peptide amphiphiles may optionally be functionalized with one or more bioactive components. Also disclosed is a A supramolecular polymer nanofiber scaffold comprising a plurality of the non-covalently bonded peptide amphiphiles, a kit including the peptide amphiphiles and a delivery device, and a method for promoting skin cell tissue regeneration by preparing peptide amphiphiles, optionally, collecting a biological material, mixing an aqueous composition, and the optional biological material with the peptide amphiphiles to produce an aqueous suspension or solution, and administering the suspension or a solution to a patient using high shear conditions.

Provided herein are implantable biomaterials for promoting regeneration of an injured biological tissue, the biomaterials including piezoelectric materials and an extracellular matrix specific to the injured biological tissue, wherein the piezoelectric materials and the extracellular matrix are electrospun together to provide tissue-specific bioactive piezoelectric nanofiber scaffolds. Also provided herein are methods of fabricating a tissue-specific bioactive piezoelectric nanofiber scaffold and methods of promoting regeneration of injured biological tissue by implanting the disclosed bioactive piezoelectric scaffolds.

This invention belongs to the field of biomedical materials technology and discloses a mechanically reinforced integrated three-dimensional micro / nanofiber scaffold and its preparation method. The invention involves electrospinning hydrophilic and hydrophobic polymer materials to obtain micro / nanofiber membranes, then foaming them using a sol-containing foaming solution to obtain a three-dimensional micro / nanofiber scaffold. The scaffold is then filled with sol and freeze-dried to obtain a mechanically reinforced integrated three-dimensional micro / nanofiber scaffold. The three-dimensional micro / nanofiber material and the freeze-dried scaffold form a continuous, integrated interpenetrating network structure with good integrity, resistance to delamination, and significantly enhanced mechanical properties. It also exhibits superior and controllable surface post-processing characteristics, especially the bifacial anisotropic treatment, which allows for convenient and quick at imparting multifunctionality to the scaffold. Furthermore, the thickness can be flexibly and conveniently adjusted through pressing during application to meet different medical needs.

A growing medium includes carboxylated cellulose nanofibers (CNFs) having an oxidation of DO > about 0.7 mmol / g and a sodium content of less than about 50 ppm or about 1.5% of a total base saturation in 1000 g of a 1 wt% CNF suspension and one or more crosslinkers. The growing medium has a water content of greater than about 90% by weight. A growing medium includes carboxylated cellulose nanofibers (CNFs) having an oxidation of DO > about 0.7 mmol / g and a sodium content of less than about 50 ppm or about 1.5% of a total base saturation in 1000 g of a 1 wt% CNF suspension and one or more crosslinkers. The growing medium is aerated and has a water content of less than about 10% by weight.

The present invention relates to scaffolds, combinations and compositions thereof that selectively remove storage damage from biological materials, particularly preserved cells. The present invention also relates to a system that selectively removes storage damage from biological materials, such as preserved blood cells. Furthermore, the present invention relates to a method for selectively removing storage damage from biological materials, such as preserved blood cells. In the present invention, the nanofiber scaffold that selectively removes storage damage from preserved cells comprises a backbone polymer functionalized with a group selected from (i) anionic groups, (ii) cationic groups, (iii) a combination of anionic and cationic groups, and (iv) zwitterionic groups.

The application belongs to the technical field of medical biomaterials, and particularly relates to a kind of poly-peptide biomimetic nanofiber scaffold with bone inductive activity and its preparation method and application.The application uses synthetic poly-peptide, and PBLG is grafted on bismuth-substituted hydroxyapatite by surface-initiated polymerization, to solve the problem of particle agglomeration and interface;Double bond-containing copolymer P[BLG co ‑(LG g ‑APEG)] is introduced, and 3D printing is used to crosslink and form covalent network to enhance mechanical properties;Combining with phase separation, ECM-like nanofiber structure is obtained.The scaffold obtained by the application has macro-pore and micro-biomimetic fiber network, and organic-inorganic hybrid components provide mineralization interface, and bismuth ion release promotes bone formation, to realize bone defect repair.

The present invention relates to a method for preparing nanofibrous scaffolds and their use for promoting cell culture. In particular, the nanofibrous scaffolds contain functionalized cellulose nanofibers that significantly improve expansion capacity and protein production. The method includes a step of splitting initial cellulose nanofibers by dispersion.

A nanofiber-based skin patch for alleviating pain, swelling, and inflammation comprising a skin adhesive layer, a nanofibrous layer comprising at least one macromolecule and a composition of natural active agents comprising a mint extract, a rose madder extract, a rosemary extract, a lavender extract, a clove extract, an arnica extract, a ginger extract, a sage extract, a ginseng extract, a garlic extract, curcumin, bromelain, honey, royal jelly, propolis, a non-woven layer configure to connect the nanofibrous layer to the skin adhesive layer such that the nanofibrous scaffold is fabricated on a top surface of non-woven layer, and a protective layer configure to protect the nanofibrous layer. The developed nanofiber-based skin patch can relieve pain, reduce swelling, and mitigate inflammation in different areas of a subject's bod without causing side effects.

An anofiber scaffold manufacturing device, suitable for manufacturing a nanofiber scaffold. The nanofiber scaffold manufacturing device includes a feeder, a delivery tube, a spinning assembly, an automatic collection device, and an electrostatic generator. The feeder is suitable for storing a spinning raw material. The delivery tube has an input end and an output end. The input end is connected to the feeder. The spinning assembly includes a spinning electrode and a nozzle. The nozzle is connected to the spinning electrode, and the spinning assembly is connected to the output end of the delivery tube. The automatic collection device is disposed opposite to the spinning assembly. The electrostatic generator is connected to the spinning electrode and forming an electric field between the spinning electrode and the automatic collection device. A nanofiber scaffold manufacturing method, a nanofiber scaffold, and an application thereof are also provided.

The invention discloses an ion-immune-electric coupling 3D bionic conductive catheter and a preparation method and application thereof, and belongs to the technical field of biomedical materials. According to the catheter, a gelatin / poly-L-lactic acid / polypyrrole nanofiber scaffold serves as a substrate, tannic acid and magnesium ions are loaded in sequence, and a three-dimensional bionic structure with electrical conductivity, ion slow release and immunoregulation functions is formed. The conductivity of the catheter is matched with that of the natural spinal cord, Mg can be continuously released to reduce excitatory toxicity of neurons, and macrophages are synergistically regulated and controlled to be polarized to M2 phenotype through tannic acid, so that oxidative stress and inflammation are relieved. In-vitro experiments show that the catheter can promote neural stem cells to differentiate into neurons and inhibit activation of astrocytes. In a rat full-cross-section spinal cord injury model, when the catheter is implanted, axon regeneration, myelin sheath formation and synaptic occurrence can be remarkably promoted, movement and urinary function recovery are effectively improved, and an innovative treatment strategy is provided for spinal cord injury repair.

This invention relates to a method for preparing a mineralized fiber scaffold composite injectable macroporous hydrogel that mimics natural bone emulsion (ECM). The method proposes preparing a PBLG fiber scaffold using electrospinning, gas foaming, and in-situ mineralization. Subsequently, the mineralized fiber scaffold composite injectable macroporous hydrogel is constructed through negative pressure perfusion, mechanical fragmentation, and gel fragmentation and reorganization. The mineralized fiber scaffold composite injectable macroporous hydrogel prepared by this invention can adapt to irregular bone defect areas, providing temporary support after implantation and offering excellent osteogenic induction and angiogenic activity for subsequent bone defect repair. The macroporous structure formed by microgel reorganization also supports the ingrowth of new tissue. This material has promising applications in bone defect repair and can also be extended to other medical fields such as cartilage defect repair and drug delivery.