96 results about "Nanofiber scaffold" patented technology

The invention is directed to a device and method to prevent migration of Human Mesenchymal Stem Cells (hMSCs) from a delivery site while allowing communication between the stem cells and native cardiomyocytes. The device is characterized by scaffold pore size, fiber diameter and biomaterial selection. The invention includes a two part polyurethane scaffold that prevents migration of stem cells, allows gap junction formation through pores and is packaged for minimally invasive delivery.

PbI2 thin film crystallization control is prerequisite of high-quality perovskite layer for the sequentially solution-processed perovskite solar cells. According to the present invention, an efficient-and-simple method has been developed by adding halogen acid additive to improve perovskite thin-film quality and an efficiency of at least 15.2% is obtained. This approach improves coverage, uniformity and stability of pervoskite thin-film. In addition, a nanofiber scaffold is incorporated into the perovskite layer so as to reduce the amount of grain boundaries, thus substaintially reducing electron recombination within these boundaries.

The invention relates to a preparation method for a conduction and sustained release type nervous tissue engineering scaffold. The method comprises the following steps: silk fibroin Silk and lactic acid-caprolactone copolymer are dissolved in a solvent, dissolved and stirred to obtain a solution, then polyaniline and camphorsulfonic acid are added and stirred and mixed uniformly to obtain a cortex electrospinning solution, and NGF (nerve growth factors) are dissolved in ultrapure water completely to obtain a core electrospinning solution; and the cortex electrospinning solution and the core electrospinning solution are accommodated in an injector respectively for coaxial electrospinning, and then fumigation treatment and vacuum drying are performed to obtain the conduction type nervous tissue engineering scaffold. According to the prepared nanofiber scaffold, the nerve repair speed is increased from ways including external electrical stimulation (conductive polymer), biochemistry (NGFs), a topological structure required by nerve regeneration (orientation guide) and the like. The method is simple to operate, good in repeatability and high in economic benefit, and provides novel experimental thought for nerve defect repair in clinical application.

An anchoring system is a combination of a nanofiber scaffold material and an arthroscopically deployable suture anchor. The anchor is deployed into a bone tunnel using common techniques. The nanofiber material extends out of the proximal end of the implant, once deployed. The implant also includes pre-loaded sutures or has the ability to accept and lock sutures to the implant. For an implant pre-loaded with suture, the implant is placed into the bone, the material is deployed above the anchor onto the surface of the bone, suture is passed through the soft tissue, and knots are tied to secure the tissue against the bone, sandwiching the material between the bone and tissue, to provide a pathway for cells from the bone marrow to the soft tissue-bone interface, promote the healing response, provide a biomimetic structure that cells readily adhere to, and create a larger healing footprint.

A self-assembling peptide constituted by D-amino acid is named as d-RAD16 and has an amino acid sequence represented by SEQ ID NO.1 in a sequence list. Test shows that hydrogel formed by the self-assembling peptide can be used for preparing a moisture keeping and water locking agent, and has the function of stopping bleeding rapidly for wound, so that the hydrogel can be used for preparing a hemostatic drug suiting clinical application by adding a pharmaceutically acceptable carrier or excipient. Nanofiber scaffold formed by the self-assembling peptide supports the growth of various cells and can be used as the substrate material for 3D cell culture. The substrate material for 3D cell culture can simulate the in-vivo environment to support the 3D growth of cells in the substrate, thereby providing a cell 3D culture drug screening mode capable of simulating the in-vivo environment and improving the success rate for the development of new drugs.

The present invention concerns a biomaterial devoid of a growth factor, comprising: —a three-dimensional scaffold made of a biocompatible polymer; and—living cells, wherein said living cells are in form of microtissues and the nanofibrous three-dimensional scaffold is a nanofibrous scaffold. It further concerns a method for manufacturing such a biomaterial. Finally, it concerns such a biomaterial for use in the treatment of a bone and / or cartilage defect.

The invention relates to a preparation method of a nervous tissue repair scaffold loaded with dual trophic factors including ganglioside (GM1) and nerve growth factor (NGF). The method comprises the processes of constructing a preparation machine, preparing GM1 and NGF ultrapure water, preparing a hexafluoroisopropanol (HFIP) mixed solution of silk fibroin and polylactic acid-polycaprolactone (P(LLA-CL)), preparing the nanofiber scaffold loaded with the dual trophic factors including the GM1 and the NGF, and the like. The prepared nanofiber scaffold reforms a neural repair conduit through a biochemical method (namely addition of the active trophic factors) and a topological structure method (namely introduction of an oriented structure), aims to research the controlled-release behaviors and the nerve regeneration promotion function of the dual trophic factors, and can induce nerve regeneration through nanofiber orientation. The method provides experimental and theoretical basis for neural repair, and provides a new method for clinical repair of large nerve defects.

A vascularized full thickness tissue engineered skin assembled by hydrogel, nanofibrous scaffolds and skin cell layers and a preparation method thereof relate to a technical field of polymer materials and biomedical materials. The artificial tissue engineered skin includes an epidermis layer and a dermis layer. The epidermal layer is formed by alternately stacking upper nanofibrous scaffolds located above the dermis layer and a kind of seed cells. The dermis layer is formed by lower nanofibrous scaffolds, the hydrogel layer above the lower nanofibrous scaffolds, and three kinds of seed cells distributed on surfaces of the lower nanofibrous scaffolds as well as inside and on a surface of the hydrogel layer. The artificial tissue engineered skin is prepared by a combination of electrospinning technology, polymer complexation technology and fiber / cell layer-gel layer-fiber / cell layer self-assembly technology. The bio-functional artificial tissue engineered skin can be used for regeneration and repair of various tissues.

The invention provides a method for preparing a composite nanofiber tissue engineering scaffold based on graphene oxide. The method includes the following steps: a step 1, dissolving silk fibroin and high-molecular polymer in a solvent with stirring till complete dissolution of the silk fibroin and the high-molecular polymer so as to acquiring a spinning solution; a step 2, performing electrostatic spinning on the spinning solution obtained from the step 1 so as to acquire a nanofiber membrane, performing steam fumigation treatment by using ethyl alcohol, and performing drying so as to acquire a silk fibroin / high-molecular polymer composite nanofiber scaffold material; and a step 3, dipping the silk fibroin / high-molecular polymer composite nanofiber scaffold material obtained from the step 2 in a graphene oxide dispersion liquid, taking out the silk fibroin / high-molecular polymer composite nanofiber scaffold material, and drying the silk fibroin / high-molecular polymer composite nanofiber scaffold material so as to acquire the composite nanofiber tissue engineering scaffold. The composite nanofiber tissue engineering scaffold is high in mechanical property, can provide biological signals for tissue growth, can promote adherence, propagation and differentiation of cells.

The invention provides highly-oriented silk fibroin based nanofiber which adopts silk fibroin as a base material, adopts a nerve growth factor as a functional material for inducing neuronal cell growth and is obtained by an in-phase spinning controller with special design through an electrospinning fiber technique. Compared with traditional nano preparation technologies such as freeze drying, gas foaming, self-assembling and the like, the nerve growth factor loaded highly-oriented silk fibroin nanofiber scaffold as an artificial nerve graft has the advantages of high efficiency, simple process, low cost and the like, has structural and functional controllability which is not likely to be realized with the traditional nanotechnology, and is better in forming effect. Meanwhile, a composite of a biological material and cells is directly spun into a composite of a bracket material and the cells once with a blended spinning technology, and compared with a tradition process of firstly bracket material preparation and later cell culture on the bracket material, the process is simple.

The invention provides a method of preparing a three-dimensional nanofiber bracket by combining an immediately crosslinking technology and an electrostatic spinning technology. The bracket prepared according to the invention is of a three dimension solid structure, the thickness of the bracket is 0.05-10mm, the interfibrous gap is 0-30 microns, and the pore size is adjustable. With the adoption of the method, the operation is simple, the relevant immediately crosslinking method solves the problems that fibers are dissolved and the structure is damaged caused by crosslinking after natural biomolecules with poorer mechanical and processing performances carry out electrostatic spinning, so that the nanofiber bracket which has a stable structure, is in three dimension solid and is loose and porous can be obtained, and a platform used for carrying cells, cell factors and drugs is prod for tissue regeneration and repair.

The invention relates to a preparation method of a nanofiber scaffold for heart tissue engineering. The preparation method comprises the steps of preparing the nanofiber scaffold from poly(glyceroi sebacate) (PGS) / a gelatin nanofiber scaffold material with different components through electrostatic spinning, and crosslinking the nanofiber scaffold in a mixed solution of hydrochlorinated N,N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to obtain the nanofiber scaffold. The nanofiber scaffold related by the invention can be used for promoting the formation of sarcomere and has wide prospects on the aspect of being used as a material for cardiac muscle cell regeneration and repair.

The invention discloses a preparation method of an antibacterial repair type electrostatic spinning collagen-bacterial cellulose composite nanofiber scaffold. The method comprises the following steps: preparing a mixed solvent, dissolving bacterial cellulose and collagen, preparing a bacterial cellulose and collagen mixed solution containing AgNO3 or gold and silver nanocluster, spinning the bacterial cellulose and collagen mixed solution containing AgNO3 or gold and silver nanocluster and other fluorescent nanomaterials by adopting an electrostatic spinning technology, and reducing a composite fiber containing the AgNO3 or gold and silver nanocluster to obtain a nanofiber scaffold containing the nano-silver or gold and silver fluorescent nanomaterials by adopting ultraviolet light illumination, wherein the related composite nanofiber scaffold is used for cell culture to prepare a tissue engineering material. Different bacterial celluloses and collagens are proportioned, and the prepared composite nanofiber scaffold realizes adjustable mechanical strength and in-vivo degrading capability and can be used for preparing tissue engineering materials of multiple purposes.

This invention relates to an artificial live bioreactor with nanofiber scaffold material as the culture medium for liver cells. A plasma inlet and an oxygen inlet are set at one end of the bioreactor hull, while a plasma outlet and an oxygen outlet are set at the other end. A nutrient solution inlet and an outlet are set at both ends of one side of the bioreactor hull, respectively. A culture medium composed of nanofiber scaffold and hollow fibers is set in the bioreactor hull. The nanofiber scaffold and the hollow fibers form coiled sandwich structure, with the nanofiber scaffold coiled into spiral drum and the hollow fibers encapsulated in the interlayer. The liver cell aggregates are adhered to the hollow fibers. An oxygen passage is set in the bioreactor to realize real-time oxygen supplement.

The invention discloses a multilayer core-shell structured nano-fiber scaffold, and a method for constructing a tissue engineering material by using the multilayer core-shell structured nano-fiber scaffold and melanocyte. The multilayer core-shell nano-fiber scaffold is prepared through electrostatic spinning, the external layer of the scaffold is a biocompatible material, the middle layer of the scaffold is a polymer barrier layer, and the core layer of the scaffold is a drug supported core material. The tissue engineering material can be formed by using the multilayer nano-fiber scaffold and the melanocyte, or using the multilayer nano-fiber scaffold, fibroblast and keratinocyte, and can be used to treat depigmentation (such as leucoderma). The multilayer core-shell structured nano-fiber scaffold has the advantages of good biocompatibility and mechanical performances, and realization of long-time controllable release of drugs, and the tissue engineering scaffold constructed by using the multilayer core-shell structured nano-fiber scaffold can effectively support growth, propagation and transplantation of melanocyte and co-culture cells.

The invention discloses a vascularized full-thickness tissue-engineered skin layer-by-layer assembled by hydrogel, nanofiber scaffolds and skin cells and a preparation method thereof, and belongs to the technical fields of macromolecule materials and biomedical materials. Artificial tissue engineered skin consists of an epidermal layer and a corium layer, wherein the epidermal layer is formed by alternately stacking nanofiber scaffolds located above the corium layer with seed cells. The corium layer consists of lower-layer nanofiber scaffolds, upper-layer hydrogel scaffolds and three kinds ofseed cells, and the seed cells are distributed on the surface of the nanofiber scaffolds, inside the hydrogel and on the surface of the hydrogel. The vascularized full-thickness tissue-engineered skinis prepared by combination of an electrospinning technology and a macromolecular complexation technology with a fiber / cell layer-gel layer-fiber / cell layer-by-layer self-assembly technology. The artificial tissue-engineered skin with biological function can be used for regeneration and repair of various tissues, especially for wound healing, angiogenesis, scar formation reduction and the like.

The invention discloses a core-shell type nano fiber bracket and a method for constructing a tissue engineering material by the core-shell type nano fiber bracket and melanocytes. A core-shell type nano fiber is prepared through electrostatic spinning; a shell layer is a biocompatible material and a core layer is in-situ cross-linked polymer hydrogel. The tissue engineering material is constructed by using the nano fiber bracket and the melanocytes or the nano fiber bracket, mechanocytes and keratinocytes, and can be used for treating depigmentation (such as leucoderma). The core-shell type nano fiber bracket disclosed by the invention has good biocompatibility and biodegradability, and can be used for loading medicines and realizing controllable releasing of the medicines; a tissue engineering bracket constructed by the core-shell type nano fiber bracket can be used for effectively supporting the growth, multiplication and transplanting of the melanocytes and co-culture cells.

The invention discloses a preparation method for a polylactic acid nanofiber scaffold functionally modified by two-dimensional nanometer black phosphorus, and aim to provide a preparation method for amultifunctional modified polylactic acid scaffold. The preparation method is characterized in that a black phosphorus nanosheet is taken as a medicine carrier to load an anti-inflammatory medicine, i.e., ibuprofen, a natural high polymer material, i.e., sodium alginate, is taken as a coating material, and through a crosslinking function of strontium ions, a medicine-carrying microsphere is obtained; an electrostatic interaction is used for evenly doping a sodium alginate medicine-carrying microsphere into a modified polylactic acid basis material which is subjected to ammonolysis and modification by branched polyethyleneimine and is rich in an active amino group; and through freezing phase separation, the modified polylactic acid scaffold with a reticular nanometer fiber structure is obtained. The preparation method has the characteristics that the prepared modified polylactic acid nanofiber scaffold embedded with the medicine-carrying microsphere is a multifunctional system with thefunctions of performing photothermal therapy, accelerating bone growth, resisting bacteria, diminishing inflammation and intelligently releasing medicines, and the polylactic acid nanofiber scaffold is an ideal bone repairing material.

The invention belongs to the field of biological functional materials, and relates to a preparation method of a reduced graphene oxide / bioglass nanofiber scaffold. The method includes the steps: mixing massive bacterial cellulose and water to prepare bacterial cellulose solution; adding reduced graphene oxide solution and performing ultrasonic treatment to obtain reduced graphene oxide / bacterial cellulose mixed solution; adding corresponding calcium, silicon and phosphorus sources into the mixed solution and performing ultrasonic treatment to obtain bacterial cellulose / reduced graphene oxide / bioglass precursor solution; calcining the bacterial cellulose / reduced graphene oxide / bioglass precursor solution in argon atmosphere after freeze-drying to obtain the reduced graphene oxide / bioglass nanofiber scaffold with an antibacterial property. Formation of hydroxyapatite can be rapidly induced in an SBF (simulated body fluid) by the aid of a unique three-dimensional network structure. The technological process is simple, operation is simple and rapid, preparation cost is low, and the prepared bioglass nanofiber scaffold is high in antibacterial property and bioactivity and has a good application prospect in the field of bone transplantation and bone replacement.

The invention relates to a preparation method of a cell-containing nanofiber bracket. The method is capable of realizing the integrated processing of degradable biological materials and cells and the cell-containing nanofiber bracket is obtained by direct high-voltage electronic injection. The preparation comprises the following steps: (1) preparing a water-soluble degradable high-molecular polymer into a homogeneous solution by deionized water, and adding beta-TCP powder, mixing and grinding by a vibrating ball mill; (2) sterilizing the material by high-temperature steam; (3) adding cell suspension into the sterilized material and stirring uniformly; and (4) extracting the cell-containing material in a micro pump by an injector, starting a high-voltage power supply and the micro pump, and setting corresponding technological parameters to carry out electronic injection so as to obtain the cell-containing nanofiber bracket. The preparation method is simple and feasible, and has important practical significance for the quick repair of bone defects.

The invention relates to a novel high-strength high-hydrophilia oxidized graphene-P34HB nanofiber scaffold and a preparing method and application thereof. The method includes the steps that (1) P34HB is modified with oxidized graphene, and an oxidized graphene-P34HB modification electrospinning solution is obtained; (2) the oxidized graphene-P34HB modification electrospinning solution is subjected to electrostatic spinning with the electrospinning technology, and the modified nanofiber membrane scaffold is obtained. According to the novel high-strength high-hydrophilia oxidized graphene-P34HB nanofiber scaffold and the preparing method and application, the P34HB is modified with the oxidized graphene for the first time, the modified biological scaffold is prepared with the electrospinning technology, the mechanical performance of the scaffold is greatly improved, and meanwhile the hydrophilia of the scaffold is improved; fibers of the scaffold are continuous, collapse and adhesion are avoided, and the porosity up to 91.4%, the high specific surface area and excellent hole communication performance are achieved; the scaffold has better biosecurity, and meanwhile has certain osteogenic induction capacity.

The invention relates to a preparation method of an alginate-hydrogel nanometer fiber scaffold. The preparation method comprises following steps: a polycaprolactone solution is prepared; a sodium alginate solution is prepared; a double-nozzle electrostatic spinning system is adopted for electrostatic spinning so as to obtain polycaprolactone-sodium alginate nanometer fiber film; the polycaprolactone-sodium alginate nanometer fiber film is immersed in a calcium chloride solution, is washed fully with an ethanol aqueous solution, is subjected to freeze drying, is immersed and washed with chloroform, is washed with ethanol and distilled water successively, and is subjected to freeze drying so as to obtain the alginate-hydrogel nanometer fiber scaffold. The alginate-hydrogel nanometer fiber scaffold possesses a stable three dimensional structure and excellent biocompatibility, can be used for 3D cell culture, and is capable of optimizing cell penetration depth.

The invention discloses a hepatic lobule-like bioreactor, which belongs to the field of biomedical equipment. A nanofiber scaffold network is arranged in a shell of the bioreactor, an intrahepatic fibrovascular network, a bile capillary network, upper hepatic bile ducts, lower hepatic bile ducts, a common bile duct, and hepatic cell collagen fiber microducts are distributed in the whole nanofiber scaffold network, and the bile capillary network is distributed at the peripheries of the hepatic cell collagen fiber microducts; the upper hepatic bile ducts and the lower hepatic bile ducts are communicated through the common bile duct; bile capillaries in the bile capillary network are provided with more than two bile capillary epidermal cell inlets; the hepatic cell collagen fiber microducts are provided with more than two hepatic cell injection ports; the intrahepatic fibrovascular network is provided with a liquid inlet and a liquid outlet; and the bile capillary epidermal cell inlets, the hepatic cell injection ports, the liquid inlet, the liquid outlet and an outlet at the lower end of the common bile duct pass through the shell. The hepatic lobule-like bioreactor truly simulates the structure of hepatic lobule, realizes the functions of metabolic detoxification, excretion and the like of livers, and is superior to the conventional bioreactor.

The invention discloses a method for culturing human melanocyte based on a nano-fiber scaffold. Melanocyte or melanocyte, fibroblast and keratinocyte can be (co-cultured) cultured and transferred by utilizing a nano-fiber scaffold which is constructed by utilizing a tissue engineering technology and has an excellent biocompatibility, and the method is applicable to depigmentation (such as leukoderma) and can be used for regulating skin color. The invention also relates to preparation of the nano-fiber scaffold and construction of a cell-nano-fiber scaffold composite material. Because the nano-fiber scaffold has a porous structure, cell culture and activity maintenance can be promoted.

The present invention relates to a device for preparing three dimensional (3D) nanofibers (blended or coaxial) materials by electrospinning. An automatic nanofiber collector device is used to control the porosity, pore size, crystallinity, geometry, the layer number and thickness of formed nanofibers. The automatic nanofiber collector device includes: (1) a collector platform; (2) a non-conductive device used to fix the collector device; (3) a plurality of electro-conductive wires or needles being pierced through the collector platform with various heights, and (4) the ends of the needles (at bottom) are wired and controlled by a microcontroller, providing forward, stand and backward movements for attached needles. The desired 3D nanofiber scaffold structures can be tailored by the micro-stepping programmable motor controller by changing the pattern and velocity of needle movement, generalized or selective needles movements, as well as intermittent versus continuous movement.

The invention discloses a breathable transparent flexible fiber-based epidermal electrode and a preparation method thereof. A motor comprises a transparent flexible nanofiber support and a transparentconductive nanometer three-dimensional network arranged above the transparent flexible nanofiber support in a filtering mode. The preparation method comprises the following steps: dissolving a high-molecular polymer in an organic solvent to obtain a uniform polymer solution; installing a polyethylene glycol terephthalate plate on a fiber receiver for receiving a fiber support; installing an injector containing a polymer spinning solution in an electrostatic spinning device for spinning to form a nanofiber support; and adding a conductive nano material into the dispersion solvent to obtain a dispersion liquid, and compounding the dispersion liquid with the polymer nanofiber support to obtain the transparent flexible fiber-based epidermal electrode. The breathable transparent flexible fiber-based epidermal electrode prepared by the invention has good conductivity and transparency, can realize the seamless fitting of the epidermal electrode and human skin, and avoids damage to the skin caused by air tightness in a long-time real-time wearing process.

An anchoring system is a combination of a nanofiber scaffold material and an arthroscopically deployable suture anchor. The anchor is deployed into a bone tunnel using common techniques. The nanofiber material extends out of the proximal end of the implant, once deployed. The implant also includes pre-loaded sutures or has the ability to accept and lock sutures to the implant. For an implant pre-loaded with suture, the implant is placed into the bone, the material is deployed above the anchor onto the surface of the bone, suture is passed through the soft tissue, and knots are tied to secure the tissue against the bone, sandwiching the material between the bone and tissue, to provide a pathway for cells from the bone marrow to the soft tissue-bone interface, promote the healing response, provide a biomimetic structure that cells readily adhere to, and create a larger healing footprint.

The present invention provides a hepatic lobule-like bioreactor. The bioreactor includes a nanofiber scaffold enclosed within a housing. An intrahepatic fibrous vascular network, a bile capillary network, upper hepatic bile ducts, lower hepatic bile ducts, a common bile duct connecting the upper and the lower hepatic bile ducts, and collagen fibrous microchannels for hepatocytes surrounded by the bile capillary network are distributed throughout the nanofiber scaffold. Bile capillaries in the bile capillary network are provided with two or more inlet ports for biliary epithelial cells. The collagen fibrous microchannels for hepatocytes are provided with two or more inlet ports for hepatocytes. The intrahepatic vascular network is provided with a liquid inlet port and a liquid outlet port. These ports extend through the housing.

The invention discloses a centrifugal gas electro-spinning device used for preparing a large number of three-dimensional nanofiber scaffolds. The device comprises a high-voltage power supply device, a gas supply device, centrifugal sprayers, open rotary receiving devices and a centrifugal drive mechanism. A liquid storage cavity is formed in each centrifugal sprayer, and thin yarn outlets are formed in the centrifugal sprayers. Each open rotary receiving device comprises a rotary shaft, a transmission device and a plurality of supporting arms. The supporting arms can form a bowl-shaped rotary face when rotating along with the rotary shafts. A plurality of the open rotary receiving devices are arranged. The centrifugal sprayers are distributed to form an annular shape in a center mode. The high-voltage power supply device and the centrifugal sprayers are connected with the open rotary receiving devices to form an electric field required by generation of solutions or melt jet flow. The centrifugal sprayers are provided with gas guide channels connected with the gas supply device so that auxiliary drawing air flow can be generated. Polymer which is jetted forms nanofibers through the electric field, the air flow and centrifugal force, compatibility to biological materials is better, the material application range is wide, and the obtained scaffold structures facilitate cell growth in tissue engineering application.

An anchoring system is a combination of a nanofiber scaffold material and an arthroscopically deployable suture anchor. The anchor is deployed into a bone tunnel using common techniques. The nanofiber material extends out of the proximal end of the implant, once deployed. The implant also includes pre-loaded sutures or has the ability to accept and lock sutures to the implant. For an implant pre-loaded with suture, the implant is placed into the bone, the material is deployed above the anchor onto the surface of the bone, suture is passed through the soft tissue, and knots are tied to secure the tissue against the bone, sandwiching the material between the bone and tissue, to provide a pathway for cells from the bone marrow to the soft tissue-bone interface, promote the healing response, provide a biomimetic structure that cells readily adhere to, and create a larger healing footprint.