28 results about "Nanofiber scaffold" patented technology

According to the invention, a bicontinuous phase conductive core-shell nanofiber membrane is applied to construction of a flexible neural electrode and a neural conduit, firstly, a bicontinuous phase conductive polymer system is constructed, and the system realizes a three-dimensional network in which an electric phase and a mechanical phase are respectively continuous, interpenetrating but independent; and the long-term technical problems of conductive component agglomeration, conductive path interruption and the like are effectively solved. And preparing the conductive nanofiber scaffold with a core-shell structure by taking a high-molecular polymer as a core layer material and a bicontinuous-phase conductive polymer system as a shell layer material through a coaxial electrostatic spinning method. The construction mode effectively establishes a stable, continuous and uniform electron conduction path, can realize effective nerve electrical stimulation and high-fidelity electrophysiological signal recording in vivo, and can be used as a nerve conduit to regulate and repair related bio-electricity signals in a nerve repair process, so as to accelerate regeneration and repair of nerves.

A nanodiamond-reinforced nanofiber scaffold system for skin regeneration, consisting of a multilayered, electrospun nanofiber matrix formed from one or more biocompatible polymers selected from polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), chitosan, gelatin or collagen, wherein nanodiamonds are present in an amount of 0.1 to 5 wt.-% are incorporated and surface-functionalized with oxygen, hydroxyl, or carboxyl groups to ensure homogeneous dispersion within the polymer matrix, the scaffold being characterized by a controlled fiber diameter in the range of 100 to 800 nm, an interconnected porosity between 60 and 90%, a tensile strength of 2 to 10 MPa, a degradation time of 2 to 8 weeks, and the ability to improve cell adhesion, proliferation, and migration of fibroblasts and keratinocytes, while simultaneously exhibiting antioxidant activity, reduced formation of reactive oxygen species, and the optional incorporation of bioactive agents selected from growth factors, antioxidants, or antimicrobial compounds to accelerate wound healing and skin tissue regeneration.

The invention provides a shape memory nano short fiber stent with self-adaptive capacity and preparation and application thereof, and belongs to the technical field of tissue engineering materials. The preparation method comprises the following steps: firstly, preparing flexible polylactic acid-fullerol composite nanofibers containing uniformly dispersed fullerol nanocrystals by utilizing an electrospinning technology, then dividing the flexible polylactic acid-fullerol composite nanofibers into short fibers by virtue of homogenization and freeze-drying processes, and assembling the short fibers into a three-dimensional porous scaffold by taking chitosan as an adhesive. The porous scaffold material disclosed by the invention has excellent biological characteristics and a water-triggered shape memory effect, and due to the fact that chitosan has excellent antioxidant activity and inherent bone induction characteristics, the nanofiber scaffold with the controllable and recoverable shape developed by the invention is expected to effectively promote osteoporotic bone healing.

A method of activating a piezoelectric neuroconduit guide scaffold including a plurality of aligned piezoelectric polymer nanofibers, the method including mechanically stimulating the piezoelectric neuroconduit guide scaffold with hydro-acoustic waves or shockwaves to remotely activate a piezoelectric effect of the nanofibrous scaffolds that induces a mechano-electrical stimulus on neural cells cultured on the scaffold, wherein the mechano-electrical stimulus promotes nerve fiber outgrowth from the neuronal cells. Some aspects relate to seeding individual components of neural tissues on the piezoelectric neuroconduit guide scaffold, wherein the hydro-acoustic stimulation induces neural tissue formation. In other aspects, the piezoelectric neuroconduit guide scaffold is implanted in a damaged neural tissue, wherein stimulating the piezoelectric neuroconduit guide scaffold by the application of shockwaves promotes nerve fiber outgrowth that bridges a nerve gap to induce nerve regeneration or reinnervation of the damaged neural tissue.

Provided herein are implantable biomaterials for promoting regeneration of an injured biological tissue, the biomaterials including piezoelectric materials and an extracellular matrix specific to the injured biological tissue, wherein the piezoelectric materials and the extracellular matrix are electrospun together to provide tissue-specific bioactive piezoelectric nanofiber scaffolds. Also provided herein are methods of fabricating a tissue-specific bioactive piezoelectric nanofiber scaffold and methods of promoting regeneration of injured biological tissue by implanting the disclosed bioactive piezoelectric scaffolds.

The application discloses a self-powered electroactive dressing with a wound repair function and a preparation method and application thereof, and belongs to the technical field of medical devices. The electroactive dressing comprises a zinc-molybdenum primary battery structure and three layers of functionalized nanofiber scaffolds. The scaffold takes gelatin-polycaprolactone as a fiber matrix, sequentially loads zinc nanoparticles, icariin and molybdenum nanoparticles to form a functional layer, and is prepared through EDC / NHS cross-linking modification. The electroactive dressing has good mechanical properties and biocompatibility, can be minimally invasively implanted in a wound site, spontaneously generates a continuous and stable microelectric field through the zinc-molybdenum primary battery, and cooperatively realizes gradient-controlled release of icariin from burst release to sustained release, so that the effects of promoting fibroblast proliferation and migration and promoting rapid wound healing are achieved. The dressing has the advantages of simple preparation process, low cost, active regulation of a wound microenvironment, mild and controllable degradation, safety, no toxic side effects, and a very good application prospect.

The invention discloses a nanofiber scaffold and a preparation method thereof, and belongs to the field of medical materials. The nanofiber scaffold comprises a matrix and nanoparticles loaded on the surface of the matrix, raw materials of the matrix comprise TPU and EPO, raw materials of the nanoparticles comprise an iron source, PDA, tetrabutyl titanate and a barium source, and the size of the nanoparticles is 170-222nm. The preparation method of the nanofiber scaffold comprises the following steps: (1) preparing PDA / Fe-BTi nanoparticles through a hydrothermal reaction; (2) preparing a matrix comprising PEO (Polyethylene Oxide) and TPU (Thermoplastic Polyurethane); and (3) loading the nano-particles on the substrate by using an electrostatic spinning technology to obtain the nano-fiber scaffold. The nanofiber scaffold provided by the invention can achieve the effects of self-generation of current by virtue of an external magnetic field, excellent mechanical property and excellent conductivity.

The invention discloses a photo-thermal immunoregulation nanofiber scaffold and a preparation method and application thereof.The photo-thermal immunoregulation nanofiber comprises nanofiber with polylactic acid as a base material, and black phosphorus nanosheets and an HSP90 inhibitor 17-DMAG are jointly loaded in the nanofiber through a high-voltage electrostatic spinning technology; the photo-thermal immunoregulation nanofiber scaffold is applied to liver cancer postoperative immunotherapy through a photo-thermal therapy method. According to the photo-thermal immune regulation and control nanofiber scaffold, a PTT effect is locally generated under near-infrared irradiation, and a strong ISR-ICD cascade reaction is driven. Meanwhile, the exposed DAMPs enhance tumor immunogenicity, recruit cytotoxic T lymphocytes and reprogram an immunosuppressive cell population, so that an immunosilence microenvironment is remodeled into a responsive tumor microenvironment and cooperates with anti-PD-L1, lasting immune memory is established, and finally recurrence and metastasis of postoperative liver cancer are effectively inhibited.

The present invention relates to a biomaterial comprising: —a membrane wound patch (a), made of a nanofibrous polymeric scaffold, —a hydrogel (b) including autologous or allogenic bone marrow-derived mesenchymal stem cells, and —a bone wound patch (c) being a nanofibrous scaffold made of polymers, wherein said scaffold has a surface coated with an interrupted coating made of multilayered droplets, said multilayered droplets being droplets composed of at least one layer pair consisting of a layer of polyanions and a layer of polycations, and wherein the bone wound patch (c) further comprises a bone growth factor; wherein the hydrogel (b) is included between the membrane wound patch (a) and the bone wound patch (c).

The invention discloses application of a composite biological material in hernia repair surgery, and belongs to the technical field of biotechnology, the composite biological material comprises a nanofiber scaffold, the scaffold is prepared by compounding polylactic acid-glycolic acid and silk fibroin, the weight ratio of the polylactic acid-glycolic acid to the silk fibroin is 70: 30, and the weight ratio of the polylactic acid-glycolic acid to the silk fibroin is 20: 30. The nanofiber scaffold is prepared through an electrostatic spinning technology and is used for providing mechanical support and promoting cell attachment and proliferation; the fiber is of a porous structure, the pore size is 10-100 microns, and cell permeation and nutrient substance exchange are facilitated; the polylactic acid-glycolic acid / silk fibroin composite scaffold is prepared by compounding polylactic acid-glycolic acid and silk fibroin, the mechanical properties of natural tissues are simulated, and a good growth scaffold is provided for cells. The gel layer is prepared through a solution phase separation method, and provides additional biological activity support and a cell attachment surface for the nanofiber scaffold. By arranging the antibacterial coating, the risk of postoperative infection is reduced, and meanwhile, protection is provided for materials and human tissues.

The present invention relates to a method for preparing a nanofibrous scaffold and its use for facilitating cell culture. In particular, the nanofibrous scaffold comprises cross-linked cellulose nanofibers that result in a continuous microcarrier material for use in industrial-scale cell culture devices.

The invention discloses a biological material for promoting optic nerve injury repair and a preparation method thereof.The biological material comprises a bionic nerve scaffold, a growth factor compound, an anti-inflammatory component and a light-operated neuroelectric signal guiding layer, the bionic nerve scaffold comprises a polycaprolactone-polyethylene glycol block copolymer and self-assembly neuropeptide, the growth factor compound comprises a neurosynaptic growth factor and a vascular endothelial growth factor, the anti-inflammatory component is liposome nanoparticles loaded with interleukin-10, and the light-operated neuroelectric signal guide layer is a polypyrrole film; the oriented nanofiber scaffold of polycaprolactone-polyethylene glycol and self-assembled neuropeptide is prepared by adopting a micro-fluidic spinning technology, a three-dimensional porous scaffold is formed to simulate a natural nerve bundle topological structure and guide oriented regeneration of axons, long-acting slow release is realized through gene activated growth factors, the axon extension efficiency is effectively improved, and the growth rate of the axons is increased. Through the interleukin-10 lipidosome anti-inflammatory component, the inflammation inhibition rate is increased, and secondary injury is relieved.

Nanofiber structures are provided as well as methods of use thereof and methods of making.

A growing medium includes carboxylated cellulose nanofibers (CNFs) having an oxidation of DO > about 0.7 mmol / g and a sodium content of less than about 50 ppm or about 1.5% of a total base saturation in 1000 g of a 1 wt% CNF suspension and one or more crosslinkers. The growing medium has a water content of greater than about 90% by weight. A growing medium includes carboxylated cellulose nanofibers (CNFs) having an oxidation of DO > about 0.7 mmol / g and a sodium content of less than about 50 ppm or about 1.5% of a total base saturation in 1000 g of a 1 wt% CNF suspension and one or more crosslinkers. The growing medium is aerated and has a water content of less than about 10% by weight.

The application belongs to the technical field of medical biomaterials, and particularly relates to a kind of poly-peptide biomimetic nanofiber scaffold with bone inductive activity and its preparation method and application.The application uses synthetic poly-peptide, and PBLG is grafted on bismuth-substituted hydroxyapatite by surface-initiated polymerization, to solve the problem of particle agglomeration and interface;Double bond-containing copolymer P[BLG co ‑(LG g ‑APEG)] is introduced, and 3D printing is used to crosslink and form covalent network to enhance mechanical properties;Combining with phase separation, ECM-like nanofiber structure is obtained.The scaffold obtained by the application has macro-pore and micro-biomimetic fiber network, and organic-inorganic hybrid components provide mineralization interface, and bismuth ion release promotes bone formation, to realize bone defect repair.

The present invention relates to a method for preparing nanofibrous scaffolds and their use for promoting cell culture. In particular, the nanofibrous scaffolds contain functionalized cellulose nanofibers that significantly improve expansion capacity and protein production. The method includes a step of splitting initial cellulose nanofibers by dispersion.

An anofiber scaffold manufacturing device, suitable for manufacturing a nanofiber scaffold. The nanofiber scaffold manufacturing device includes a feeder, a delivery tube, a spinning assembly, an automatic collection device, and an electrostatic generator. The feeder is suitable for storing a spinning raw material. The delivery tube has an input end and an output end. The input end is connected to the feeder. The spinning assembly includes a spinning electrode and a nozzle. The nozzle is connected to the spinning electrode, and the spinning assembly is connected to the output end of the delivery tube. The automatic collection device is disposed opposite to the spinning assembly. The electrostatic generator is connected to the spinning electrode and forming an electric field between the spinning electrode and the automatic collection device. A nanofiber scaffold manufacturing method, a nanofiber scaffold, and an application thereof are also provided.

The invention discloses an ion-immune-electric coupling 3D bionic conductive catheter and a preparation method and application thereof, and belongs to the technical field of biomedical materials. According to the catheter, a gelatin / poly-L-lactic acid / polypyrrole nanofiber scaffold serves as a substrate, tannic acid and magnesium ions are loaded in sequence, and a three-dimensional bionic structure with electrical conductivity, ion slow release and immunoregulation functions is formed. The conductivity of the catheter is matched with that of the natural spinal cord, Mg can be continuously released to reduce excitatory toxicity of neurons, and macrophages are synergistically regulated and controlled to be polarized to M2 phenotype through tannic acid, so that oxidative stress and inflammation are relieved. In-vitro experiments show that the catheter can promote neural stem cells to differentiate into neurons and inhibit activation of astrocytes. In a rat full-cross-section spinal cord injury model, when the catheter is implanted, axon regeneration, myelin sheath formation and synaptic occurrence can be remarkably promoted, movement and urinary function recovery are effectively improved, and an innovative treatment strategy is provided for spinal cord injury repair.

This invention relates to a method for preparing a mineralized fiber scaffold composite injectable macroporous hydrogel that mimics natural bone emulsion (ECM). The method proposes preparing a PBLG fiber scaffold using electrospinning, gas foaming, and in-situ mineralization. Subsequently, the mineralized fiber scaffold composite injectable macroporous hydrogel is constructed through negative pressure perfusion, mechanical fragmentation, and gel fragmentation and reorganization. The mineralized fiber scaffold composite injectable macroporous hydrogel prepared by this invention can adapt to irregular bone defect areas, providing temporary support after implantation and offering excellent osteogenic induction and angiogenic activity for subsequent bone defect repair. The macroporous structure formed by microgel reorganization also supports the ingrowth of new tissue. This material has promising applications in bone defect repair and can also be extended to other medical fields such as cartilage defect repair and drug delivery.

A growing medium includes carboxylated cellulose nanofibers (CNFs) having an oxidation of DO > about 0.7 mmol / g and a sodium content of less than about 50 ppm or about 1.5% of a total base saturation in 1000 g of a 1 wt% CNF suspension and one or more crosslinkers. The growing medium has a water content of greater than about 90% by weight. A growing medium includes carboxylated cellulose nanofibers (CNFs) having an oxidation of DO > about 0.7 mmol / g and a sodium content of less than about 50 ppm or about 1.5% of a total base saturation in 1000 g of a 1 wt% CNF suspension and one or more crosslinkers. The growing medium is aerated and has a water content of less than about 10% by weight.

The invention discloses a fibrous membrane loaded with infinite coordination polymer nanoparticles and a preparation method and application thereof, the fibrous membrane loaded with infinite coordination polymer nanoparticles comprises a nanofiber scaffold fused with polycaprolactone (PCL) and sericin Se, and curcumin-copper ICP loaded on the nanofiber scaffold. Accurate pH response release is shown, in an early infected / inflammatory wound (acidic microenvironment), curcumin Cur and Cu < 2 + > are released through rapid dissociation of curcumin-copper ICP, curcumin Cur clears ROS (reactive oxygen species) and inhibits inflammation, and Cu < 2 + > has a synergistic antimicrobial effect. Along with the transition of the wound to a neutral healing stage, the structure of the curcumin-copper ICP is re-stabilized, and the release rate is obviously slowed down. In the stage, low-dose curcumin Cur maintains the anti-inflammatory / anti-oxidation effect, and slow Cu < 2 + > release promotes angiogenesis. The intelligent controlled release provides a reference for designing an environmentally responsive drug delivery system.

The invention discloses a magnetic system for bone regeneration and a preparation method and application thereof, the magnetic system comprises an inner magnet and an outer magnet, the inner magnet is a polycaprolactone / polylactic acid electrostatic spinning nanofiber scaffold loaded with multifunctional magnetic nanoparticles, and the outer magnet is a static magnetic field generated by a neodymium magnet. The multifunctional magnetic nano-particles are ZnFe2O4 nano-particles of which the surfaces are loaded with dopamine packages, and the polycaprolactone / polylactic acid is a mixed solution of polycaprolactone and polylactic acid. According to the invention, the magnetic biological effect of ZnFe2O4 is utilized to enhance the specific expression of osteoblasts, the magnetic effect is enhanced through an external static magnetic field, and Zn < 2 + > released by ZnFe2O4 also has osteogenesis and antibacterial capabilities. Through dopamine surface modification, ZnFe2O4 nanoparticles are prevented from being gathered in cells, the oxidation resistance of the nanoparticles is improved, the magnetic nanoparticles are multifunctional, the microenvironment under the bone defect condition is effectively coped with, and then osteogenesis is promoted.

The invention provides an immunoregulation-osteogenesis bifunctional nanofiber scaffold as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering materials. P24 peptide is accurately encapsulated in a poly-L-lactic acid based core-shell nanofiber core by adopting a micro-sol technology, and meanwhile, an active factor SDF-1 is covalently fixed on the surface of a fiber shell. Then the outer surface of the fiber is coated with a polydopamine coating, the biological activity and adhesion of the fiber and a titanium screw are further enhanced, and finally a multifunctional screw system (S-MS-PDA-Ti) integrating mechanical and biological fixing strategies is constructed. An in-vitro research shows that the S-MS-PDA-Ti keeps good mechanical stability; the PDA coating enhances nail-holding power and relieves interface inflammation, rapid release of SDF-1 promotes collection and proliferation of MSCs, and continuous release of P24 peptide promotes formation of an osteogenesis or cartilage microenvironment. Animal experiments further prove that the material can remarkably improve the anchoring strength of screws and promote bone interface osteogenesis in a rat OP model.

The invention discloses a preparation method of an articular cartilage three-dimensional nanofiber scaffold, and relates to the technical field of medical biological materials. The method comprises the following steps: dissolving a degradable polymer and a natural polymer material in a solvent to prepare a spinning solution; performing electrostatic spinning on the spinning solution to obtain a cartilage scaffold two-dimensional nanofiber membrane; performing foaming treatment on the cartilage scaffold two-dimensional nanofiber membrane, and converting the cartilage scaffold two-dimensional nanofiber membrane into a three-dimensional nanofiber cartilage scaffold; and soaking the freeze-dried three-dimensional nanofiber cartilage scaffold in a mixed solution of epigallocatechin gallate and SrCl2 for chemical modification, and freeze-drying the three-dimensional nanofiber cartilage scaffold to obtain a finished product of the three-dimensional nanofiber cartilage scaffold. The articular cartilage three-dimensional nanofiber cartilage scaffold prepared by the method can effectively simulate a three-dimensional ECM structure of natural cartilage, and cartilage regeneration is facilitated.

The application discloses a lacrimal passage epithelial organoid and a construction method and application thereof, and the construction method comprises the following steps: S1, preparation of a three-dimensional biomimetic support; S2, acquisition of lacrimal passage epithelial stem cells; and S3, construction and culture of the organoid. The PCL / GEL nanofiber support constructed by the electrospinning technology is combined with Matrigel coating, the extracellular matrix (ECM) microenvironment of the in-vivo lacrimal passage mucosa is successfully simulated, and the technical difficulties that stem cells are prone to aging and differentiation direction disorder in traditional two-dimensional culture are fundamentally solved. This breakthrough result promotes the lacrimal passage tissue engineering from simple 'cell adhesion growth' to a new height of'structure-function synchronous reconstruction', and provides a double technical guarantee of seed cells and tissue engineering supports for solving the clinical core pain point of postoperative scar adhesion and re-occlusion of the lacrimal passage obstruction disease.

This application relates to the field of polymer materials, specifically disclosing an organoclay-modified epoxidized natural latex insulating sleeve and its preparation method. The insulating sleeve comprises the following raw materials in parts by weight: 100 parts of epoxidized natural rubber latex concentrate, with an epoxidation degree of 50%; 1 part organoclay, Cloisite 30B; 2.7–3.0 parts dispersant; 1.0 part sulfur; 0.8 parts vulcanization accelerator; 1.0 part zinc oxide; 0.5 parts stearic acid; 1.0 part antioxidant; 2.0 parts plasticizer; 0.5–1 part glyceryl monostearate; 2–4 parts potassium hexatitanate whiskers; and 2–4 parts nano-hydroxyapatite nanofiber scaffold. The preparation method includes: organoclay pre-dispersion treatment; preparation of epoxidized natural composite latex; latex curing; sleeve molding and post-treatment. The insulating sleeve of this application simultaneously achieves good mechanical properties, flexibility, and aging resistance.

The present invention relates to a method for preparing a nanofibrous scaffold and use of the same for promoting cell culturing. In particular, the nanofibrous scaffold comprises functionalized cellulose nanofibers which significantly increases expansion capacity and protein production. The method comprises the step of dividing the initial cellulose nanofibers by dispersing.

The invention discloses a nanofiber scaffold and a preparation method thereof, and belongs to the technical field of biomedical materials. The preparation method of the nanofiber scaffold comprises the following steps: taking two parts of nanofiber membranes, respectively carrying out glutaraldehyde steam crosslinking treatment to obtain a crosslinked first nanofiber material, and carrying out thermal crosslinking treatment to obtain a crosslinked second nanofiber material; the preparation method comprises the following steps: homogeneously dispersing a first nanofiber material and a second nanofiber material in a freeze-drying solvent according to a mass ratio of 1: (0.2-5) to obtain nanofiber dispersion liquid; performing freeze drying and vacuum drying on the nanofiber dispersion liquid to obtain a nanofiber scaffold; wherein the freeze-drying solvent in the dispersion liquid of the first nanofiber material always contains tert-butyl alcohol. The nanofiber scaffold prepared by the method has good structural stability and appearance quality.