Preparation method of cell-containing nanofiber bracket

A nanofiber and cell technology, used in medical science, prosthesis, etc., can solve the problems of difficult to achieve cell fixed-point distribution, slow growth of new tissue, low cell planting density, etc. good controllability

Inactive Publication Date: 2015-04-08
上海蓝衍生物科技有限公司
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tissue engineering nanofiber scaffold obtained by this method has the following disadvantages: 1) The cell planting density is small, which makes the growth rate of new tissue slow
2) Tissues generally contain a variety of cells, and the fixed-point distribution of different types of cell

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of cell-containing nanofiber bracket

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for preparing a cell-containing nanofiber scaffold. The nanofiber scaffold is composed of degradable polymers and cells, and is prepared at one time by electrospray technology, including the following steps:

[0020] 1) Use an electronic scale to weigh 1.76 grams of PVA particles and slowly add them to 18.24 grams of deionized water under stirring conditions. After fully swelling, heat up to about 90°C to accelerate dissolution, and keep warm for 3 hours to obtain transparent 8.8 After the %PVA solution, add 0.15 grams of β-TCP powder to mix and grind for 2 hours with a vibrating ball mill;

[0021] 2) High-temperature disinfection of the above materials at 120°C;

[0022] 3) Human adipose stem cells of passage 4 were digested with 0.25% trypsin and collected in a 50mL centrifuge tube, centrifuged at 1000 r / min for 5 min, discarded the supernatant, and transferred the cell suspension to the sterilized above materials and stirred evenly to obtain Cell-containin...

Embodiment 2

[0028] This example is basically the same as Example 1, except that: Weigh 2 grams of PEO particles with an electronic balance, slowly add them to 8 grams of deionized water under stirring, and heat up to about 60°C to accelerate dissolution after fully swelling. And keep it warm for 3 hours, after making a transparent 20% PEO solution, add 0.15 g of β-TCP powder for mixing and grind for 2 hours with a vibrating ball mill.

Embodiment 3

[0030] This example is basically the same as Example 1, except that the stem cells used in step 3) are bone marrow mesenchymal stem cells.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of a cell-containing nanofiber bracket. The method is capable of realizing the integrated processing of degradable biological materials and cells and the cell-containing nanofiber bracket is obtained by direct high-voltage electronic injection. The preparation comprises the following steps: (1) preparing a water-soluble degradable high-molecular polymer into a homogeneous solution by deionized water, and adding beta-TCP powder, mixing and grinding by a vibrating ball mill; (2) sterilizing the material by high-temperature steam; (3) adding cell suspension into the sterilized material and stirring uniformly; and (4) extracting the cell-containing material in a micro pump by an injector, starting a high-voltage power supply and the micro pump, and setting corresponding technological parameters to carry out electronic injection so as to obtain the cell-containing nanofiber bracket. The preparation method is simple and feasible, and has important practical significance for the quick repair of bone defects.

Description

technical field [0001] The invention relates to a method for preparing a cell-containing nanofiber scaffold, which belongs to the field of tissue engineering nanofiber scaffold preparation and repair. Background technique [0002] The electrospinning method is a method that can quickly and easily prepare nanofiber scaffolds that has emerged in recent years. The scaffold materials prepared by this technology have high porosity and good channel connectivity, which can well build a microenvironment for cell growth. Therefore, nanofibrous scaffolds are widely used in the medical field. The current common method for constructing nanofibrous tissue engineering nanofiber scaffolds is: first prepare nanofiber scaffolds, and then plant cells on the surface of the scaffolds. However, the tissue engineering nanofiber scaffold obtained by this method has the following disadvantages: 1) The cell planting density is small, which makes the growth rate of new tissue slow. 2) Tissues gener...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/38A61L27/18A61L27/16A61L27/50
Inventor 胡庆夕陈海萍刘媛媛向科
Owner 上海蓝衍生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap