Preparation method of alginate-hydrogel nanometer fiber scaffold

A nanofiber, alginate technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of not easy displacement, three-dimensional displacement, etc., to optimize the penetration depth, ensure controllability, and good biocompatibility Effect

Active Publication Date: 2018-06-15
山东省日照市人民医院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current technology to prepare three-dimensional cell scaffolds and provide an approximate cell growth environment is not mature. Due to the unstable position of cells and the porosity of fibers, it is easy for cells to generate horizontal displacement in three dimensions, but it is not easy to achieve displacement in three dimensions.

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  • Preparation method of alginate-hydrogel nanometer fiber scaffold
  • Preparation method of alginate-hydrogel nanometer fiber scaffold
  • Preparation method of alginate-hydrogel nanometer fiber scaffold

Examples

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Embodiment 1

[0037] A kind of preparation method of alginate-hydrogel nanofiber support, described preparation method comprises the following steps:

[0038] 1) Mix chloroform and methanol in a volume ratio of 3:1, dissolve polycaprolactone in the chloroform / methanol mixture, and prepare a polycaprolactone solution with a polycaprolactone content of 16 wt %;

[0039] 2) Mix distilled water and dimethyl sulfoxide at a volume ratio of 19:1, dissolve sodium alginate and TritonX-100 in the mixture of distilled water and dimethyl sulfoxide, and prepare a sodium alginate solution. The content of the sodium alginate in the solution is 2wt%, and the content of the Triton X-100 in the solution is 0.5wt%;

[0040] 3) Using a double-nozzle electrospinning system, weigh 18g of the polycaprolactone solution prepared in step 1) and place it in the outer nozzle of the double-nozzle electrospinning system, weigh 20g of the sodium alginate solution prepared in step 2) and add it to the double Electrospinn...

Embodiment 2

[0047] A kind of preparation method of alginate-hydrogel nanofiber support, described preparation method comprises the following steps:

[0048] 1) Mix chloroform and methanol in a volume ratio of 3:1, dissolve polycaprolactone in the chloroform / methanol mixture, and prepare a polycaprolactone solution with a polycaprolactone content of 16 wt %;

[0049] 2) Mix distilled water and dimethyl sulfoxide at a volume ratio of 19:1, dissolve sodium alginate and TritonX-100 in the mixture of distilled water and dimethyl sulfoxide, and prepare a sodium alginate solution. The content of the sodium alginate in the solution is 2wt%, and the content of the Triton X-100 in the solution is 0.5wt%;

[0050] 3) Using a double-nozzle electrospinning system, weigh 18g of the polycaprolactone solution prepared in step 1) and place it in the outer nozzle of the double-nozzle electrospinning system, weigh 20g of the sodium alginate solution prepared in step 2) and add it to the double Electrospinn...

Embodiment 3

[0056] A kind of preparation method of alginate-hydrogel nanofiber support, described preparation method comprises the following steps:

[0057] 1) Mix chloroform and methanol in a volume ratio of 3:1, dissolve polycaprolactone in the chloroform / methanol mixture, and prepare a polycaprolactone solution with a polycaprolactone content of 16 wt %;

[0058] 2) Mix distilled water and dimethyl sulfoxide at a volume ratio of 19:1, dissolve sodium alginate and TritonX-100 in the mixture of distilled water and dimethyl sulfoxide, and prepare a sodium alginate solution. The content of the sodium alginate in the solution is 2wt%, and the content of the Triton X-100 in the solution is 0.5wt%;

[0059] 3) Using a double-nozzle electrospinning system, weigh 18g of the polycaprolactone solution prepared in step 1) and place it in the outer nozzle of the double-nozzle electrospinning system, weigh 20g of the sodium alginate solution prepared in step 2) and add it to the double Electrospinn...

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Abstract

The invention relates to a preparation method of an alginate-hydrogel nanometer fiber scaffold. The preparation method comprises following steps: a polycaprolactone solution is prepared; a sodium alginate solution is prepared; a double-nozzle electrostatic spinning system is adopted for electrostatic spinning so as to obtain polycaprolactone-sodium alginate nanometer fiber film; the polycaprolactone-sodium alginate nanometer fiber film is immersed in a calcium chloride solution, is washed fully with an ethanol aqueous solution, is subjected to freeze drying, is immersed and washed with chloroform, is washed with ethanol and distilled water successively, and is subjected to freeze drying so as to obtain the alginate-hydrogel nanometer fiber scaffold. The alginate-hydrogel nanometer fiber scaffold possesses a stable three dimensional structure and excellent biocompatibility, can be used for 3D cell culture, and is capable of optimizing cell penetration depth.

Description

technical field [0001] The invention belongs to the field of nanofiber scaffolds, and in particular relates to a preparation method of alginate-hydrogel nanofiber scaffolds. Background technique [0002] Electrospun nanofibers are commonly used scaffold materials for mimicking extracellular matrix structures in tissue engineering. However, whether the cell culture environment of the nanofiber mesh is representative of the real in vivo environment remains unclear. Because of the intermingling of the cells themselves and the porous nature of the electrospun nanofibers, the cells can migrate horizontally on the grid, which interferes with the three-dimensional displacement of the cells on the nanofiber scaffold. Since the difference between 2D and 3D cell culture environments has obvious effects on cell metabolism, 3D cell culture has become a hotspot for tumor tissue modeling and stem cell culture applications. For example, the Chinese patent "Preparation method of three-dim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/52A61L27/56B82Y5/00B82Y30/00D01D5/00D04H1/4382
CPCA61L27/20A61L27/52A61L27/56A61L2400/08A61L2400/12B82Y5/00B82Y30/00D01D5/003D01D5/0076D01D5/0092D04H1/4382C08L5/04
Inventor 郭军周兆娜陈为志
Owner 山东省日照市人民医院
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