Preparation method of nervous tissue repair scaffold loaded with dual trophic factors including ganglioside (GM1) and nerve growth factor (NGF)

A nutrient factor and nerve tissue technology, which is applied in the field of preparation of nerve tissue repair scaffolds, achieves the effects of stable preparation process, few process parameters, and simple preparation device

Inactive Publication Date: 2013-05-08
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the preparation of ganglioside GM1, nerve growth factor, and NGF dual trophic factor-loaded nanofibers based on coaxial electrospinning technology, and finally the preparation of active nerve repair catheters loaded with GM1 and NGF dual trophic factors

Method used

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  • Preparation method of nervous tissue repair scaffold loaded with dual trophic factors including ganglioside (GM1) and nerve growth factor (NGF)
  • Preparation method of nervous tissue repair scaffold loaded with dual trophic factors including ganglioside (GM1) and nerve growth factor (NGF)
  • Preparation method of nervous tissue repair scaffold loaded with dual trophic factors including ganglioside (GM1) and nerve growth factor (NGF)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 0.20 g of this protein silk fibroin, 0.60 g of polylactic acid-polycaprolactone, dissolve in 10 ml of hexafluoroisopropanol (HFIP), and the molecular weight of polylactic acid-polycaprolactone is M w ≈300,000, stir magnetically at a rate of 400 rpm for 12 hours and mix evenly to obtain a shell electrospinning solution with a total concentration of 8%; take 10 micrograms per milliliter of ganglioside (GM1) ultrapure aqueous solution and 10 micrograms per milliliter 1 ml of ultrapure aqueous solution of nerve growth factor (NGF) was mixed uniformly to obtain 2 ml of GM1 and NGF concentration ratio of 1:1 mixed solution to obtain the core layer electrospinning solution. The shell layer electrospinning solution was loaded into a syringe, and the advancing speed of the micro-injection pump was controlled to be 1 ml per hour, and the core layer electrospinning solution was filled into another syringe, and the advancing speed of the micro-injection pump was controlled to ...

Embodiment 2

[0030] Weigh 0.25 grams of protein silk fibroin, 0.75 grams of polylactic acid-polycaprolactone, dissolve in 10 ml of hexafluoroisopropanol (HFIP), and the molecular weight of polylactic acid-polycaprolactone is M w ≈300,000, stir magnetically at a rate of 600 rpm for 12 hours and mix evenly to obtain a shell electrospinning solution with a total concentration of 10%; take 20 micrograms per milliliter of ganglioside (GM1) ultrapure aqueous solution and 20 micrograms per milliliter 1 ml of ultrapure aqueous solution of nerve growth factor (NGF) was mixed uniformly to obtain 2 ml of GM1 and NGF concentration ratio of 1:1 mixed solution to obtain the core layer electrospinning solution. The shell layer electrospinning solution was loaded into a syringe, and the advancing speed of the micro-injection pump was controlled to be 1 ml per hour, and the core layer electrospinning solution was filled into another syringe, and the advancing speed of the micro-injection pump was controlled...

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Abstract

The invention relates to a preparation method of a nervous tissue repair scaffold loaded with dual trophic factors including ganglioside (GM1) and nerve growth factor (NGF). The method comprises the processes of constructing a preparation machine, preparing GM1 and NGF ultrapure water, preparing a hexafluoroisopropanol (HFIP) mixed solution of silk fibroin and polylactic acid-polycaprolactone (P(LLA-CL)), preparing the nanofiber scaffold loaded with the dual trophic factors including the GM1 and the NGF, and the like. The prepared nanofiber scaffold reforms a neural repair conduit through a biochemical method (namely addition of the active trophic factors) and a topological structure method (namely introduction of an oriented structure), aims to research the controlled-release behaviors and the nerve regeneration promotion function of the dual trophic factors, and can induce nerve regeneration through nanofiber orientation. The method provides experimental and theoretical basis for neural repair, and provides a new method for clinical repair of large nerve defects.

Description

technical field [0001] The invention belongs to the field of biomaterial scaffolds for repairing nerve defects and the preparation thereof, in particular to a method for preparing nerve tissue repair scaffolds loaded with GM1 and NGF dual nutritional factors. Background technique [0002] Peripheral nerve injury is a common clinical disease. Clinically, for the severed peripheral nerve, microsurgical techniques are often used for end-to-end adventitial suture, perineural suture or combined suture to restore the continuity of the nerve. However, autologous nerve grafting is still the "gold standard" for repair. In recent years, the rise of tissue engineering has brought hope to the treatment of many tissue defects and organ failure patients. [0003] Nerve regeneration conduits constructed of biomaterials can provide a favorable space for nerve cells to obtain nutrients, grow and metabolize. However, nerve regeneration is the result of the interaction between cells, extrace...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/22A61L27/54
Inventor 莫秀梅贺梨萍吴瑃辰
Owner DONGHUA UNIV
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