Device and method for continuous preparation of microbe-embedded microspheres

Abstract

The invention discloses a microbe embedded microsphere continuous preparation device and method. The device comprises a dispenser, a stepping motor, a needle cylinder, a funnel, a bottle, a centrifuge tube and a rotating plate, wherein the bottle is arranged on the rotating plate; the centrifuge tube is arranged in the bottle; the upper part of the centrifuge tube is provided with a rubber plug; a cannula of the funnel penetrates through the rubber plug and extends into the centrifuge tube; the conical part of the funnel is clamped at the bottle opening; one side of the rotating plate is provided with the stepping motor; a screw rod of the stepping motor is vertically arranged on one side of the rotating plate; a slider is connected with the needle cylinder through a connecting rod; the needle cylinder is vertically arranged above the funnel; needle heads are faced down; and the tail end opening of the needle cylinder is connected with an output gas pipe of the dispenser. The method has the advantages of simple technological conditions for microsphere preparation, accessible equipment, mild conditions for bioactive substances in the manufacturing process, and higher diameter uniformity of the obtained microspheres, and can implement automation in the preparation process. The complete preparation device is simple, and can be integrally arranged in a common superclean bench to prevent infectious microbe pollution. The device and method can be used for preparing microbe cell embedded microspheres.

Description

Technical field: [0001] The invention belongs to the field of microorganisms, and in particular relates to a continuous preparation device and method for microorganism-embedded microspheres. Background technique: [0002] By physical or chemical methods, free microorganisms are immobilized in a limited space area to keep them active and able to reproduce and expand, and this immobilization medium has good permeability, so that nutrients or signal molecules between microorganisms can be obtained Exchange, and finally can be enriched and separated to obtain microbial species that cannot be obtained by traditional separation methods. Abroad, Ben and Zengler combined micro-embedding with in-situ environmental culture to conduct high-throughput culture of seawater, seabed sediments, and coral symbiotic microorganisms, which greatly improved the cultivability of marine microorganisms under laboratory conditions. Eitan compared the technology with traditional culture technology an...

AI Technical Summary

Problems solved by technology

The process of making microspheres by traditional extrusion method is relatively simple, but it is difficult to prepare microspheres with small particle size, which cannot meet the high-throughput sorting after microbial co-culture

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