Preparation method of conductive slow-release nerve tissue engineering scaffold

A nerve tissue, slow-release technology, which is applied in the preparation of conductive slow-release nerve tissue engineering scaffolds and the field of nerve defect repair, achieves the effects of good biocompatibility, few control parameters, and simple preparation device

Active Publication Date: 2016-09-07
诺一迈尔(山东)医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, nerve growth factor (NGF) can also promote the regeneration of motor nerves. So far, there is no preparation of a conductive nerve growth factor (NGF) loaded nerve growth factor (NGF) based on coaxial electrospinning technology. Reports on Tissue Engineering Scaffolds

Method used

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  • Preparation method of conductive slow-release nerve tissue engineering scaffold
  • Preparation method of conductive slow-release nerve tissue engineering scaffold
  • Preparation method of conductive slow-release nerve tissue engineering scaffold

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Weigh 0.1g of protein silk fibroin, 0.3g of lactic acid-caprolactone copolymer, dissolve in 5ml of hexafluoroisopropanol (HFIP), the molecular weight of lactic acid-caprolactone copolymer is Mw≈300,000, at 200r / min. Stir at a high speed for 5 hours and mix evenly to obtain a solution with a total concentration of 8% (w / v); after mixing evenly, add 60mg of polyaniline (Mn≈15,000) and camphorsulfonic acid, and then magnetically stir at a rate of 300r / min. After 48 hours, the cortex electrospinning solution was prepared by mixing evenly, and 1 ml of 10 μg / ml nerve growth factor (NGF) ultrapure aqueous solution was taken as the core electrospinning solution. The electrospinning solution of the shell layer was loaded into a syringe, and the advancing speed of the micro-injection pump was controlled to be 1ml / h. The electrospinning solution of the core layer was loaded into another syringe, and the advancing speed of the micro-injection pump was controlled to be 0.2ml / h. The ...

Embodiment 2

[0037]Weigh 0.125g of protein silk fibroin, 0.375g of lactic acid-caprolactone copolymer, dissolve in 5ml hexafluoroisopropanol (HFIP), the molecular weight of lactic acid-caprolactone copolymer is Mw≈300,000, at 200r / min. Stir at a high speed for 5 hours and mix evenly to obtain a solution with a total concentration of 10% (w / v); after mixing evenly, add 80 mg of polyaniline (Mn≈15,000) and camphorsulfonic acid, and then magnetically stir at a rate of 300r / min. After 48 hours, the cortex electrospinning solution was prepared by mixing evenly, and 1 ml of 10 μg / ml nerve growth factor (NGF) ultrapure aqueous solution was taken as the core electrospinning solution. The electrospinning solution of the shell layer was loaded into a syringe, and the advancing speed of the micro-injection pump was controlled to be 1ml / h. The electrospinning solution of the core layer was loaded into another syringe, and the advancing speed of the micro-injection pump was controlled to be 0.2ml / h. Th...

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Abstract

The invention relates to a preparation method of a conductive slow-release nerve tissue engineering scaffold, comprising: dissolving silk fibroin protein Silk and lactic acid-caprolactone copolymer in a solvent, dissolving and stirring to obtain a solution, and then adding polyaniline PANi and camphor Sulfonic acid, stirred and mixed to obtain cortex electrospinning solution; nerve growth factor NGF was completely dissolved in ultrapure water to obtain core layer electrospinning solution; cortex electrospinning solution and core layer electrospinning solution were packed separately into a syringe for coaxial electrospinning, fumigation, and vacuum drying to obtain a conductive neural tissue engineering scaffold. The nanofibrous scaffold prepared by the present invention promotes the speed of nerve repair through ways such as external electrical stimulation of conductive polymers, biochemical nerve growth factors, and topological structure orientation guidance required for nerve regeneration. The method of the invention is simple in operation, good in repeatability and high in economic benefit, and provides new experimental ideas for repairing nerve defects encountered in clinical practice.

Description

technical field [0001] The invention belongs to the field of nerve defect repair involving the preparation of biological materials, in particular to a preparation method of a conductive slow-release nerve tissue engineering scaffold. Background technique [0002] Peripheral nerve regeneration and functional recovery have always been a clinical problem. Every year, a large number of patients around the world need nerve repair. By far, the most commonly used method is autologous nerve transplantation to treat nerve rupture or defect. However, the sources of autologous nerves available for transplantation are very limited. In addition, it is difficult to avoid the misalignment of nerve fibers during microsurgery, which will affect the recovery of nerve function. In recent years, with the gradual development of tissue engineering, the use of biomaterials to construct nerve regeneration conduits has brought hope for clinical nerve defect repair. [0003] However, peripheral ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61L27/54A61L27/50A61L27/22A61L27/18D01D5/00D04H1/728D04H1/541
Inventor 莫秀梅张建光孙彬彬宋炜
Owner 诺一迈尔(山东)医学科技有限公司
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