147results about How to "Promotes regenerative repair" patented technology

The invention relates to a bone repair porous bracket, which comprises a substrate with a bionic porous structure and growth factor controlled-release microspheres, wherein the growth factor controlled-release microspheres are adsorbed into gaps of the bionic porous structure on the substrate or are dispersed into the substrate by being uniformly mixed with substrate raw material in a substrate forming process. The invention further provides a rapid forming method for the bone repair porous bracket. The method comprises the following steps of: preparing growth factor controlled-release microspheres; forming a substrate; and adsorbing. The invention further provides another rapid forming method for the bone repairing porous bracket. The method comprises a step for preparing growth factor controlled-release microspheres and a step for forming a substrate. The growth factor controlled-release microspheres are introduced into the bracket, bone growth can be induced under the action of the controlled release of growth factors, and inward bone tissue growth is facilitated through a porous structure formed by degradation of the controlled-release microspheres, so that bone tissue regeneration repair is achieved, and bone healing is facilitated effectively. Three-dimensional printing is performed by adding controlled-release microspheres and adopting a rapid forming technology, and a forming process is simple and rapid.

The invention provides a drug-loading type guided tissue regeneration membrane and preparation method thereof. Polycaprolactone and other biodegradable aliphatic polyesters are used as raw materials and added with an antibacterial drug, and the materials are prepared into a single-layer guided tissue regeneration membrane through an electrospinning method. Or, polycaprolactone and degradable aliphatic polyester material are used as a dense layer, degradable natural polymer material and bio active particles are used as a loose layer, and an antibacterial drug is added to the dense layer; and the layers are prepared into a double-layer guided tissue regeneration membrane with different pore structures and biological activity through a layer by layer electrostatic spinning method. The membrane material provided by the invention has excellent biocompatibility, mechanical properties and degradation property consistent with tissue repair process, can effectively prevent the growth of fibroblasts towards tissue defects, at the same time promote the regeneration repair of tissue, does not have to taken out through a secondary operation; and the membrane material can effectively inhibit bacteria infection and inflammation after the operation, can be widely used in medical fields, such as guided tissue regeneration, postoperative adhesion prevention and drug delivery membrane.

The invention provides marine biomaterial composite hydrogel dressing and a preparation method thereof and relates to the technical field of medical dressing. The hydrogel dressing is prepared from asodium alginate-fish collagen mixed solution and a chitosan-crosslinking agent mixed solution, wherein the sodium alginate-fish collagen mixed solution is prepared from sodium alginate, fish collagen,polyvinyl alcohol, sodium carboxymethyl cellulose, pectin, glycerin, propanediol and distilled water; the chitosan-crosslinking agent mixed solution is prepared from chitosan, a crosslinking agent, glacial acetic acid and distilled water. The invention also provides the preparation method of the dressing. The sodium alginate-fish collagen mixed solution is cultured at the constant temperature, the chitosan-crosslinking agent mixed solution is added, the mixture reacts, and the hydrogel dressing is obtained. The hydrogel dressing has excellent mechanical property, moisture retention and biocompatibility, accelerates wound repairing, can diminish inflammation and perform sterilization, has no wound inflammation reaction, effectively promotes wound healing, is easy to remove and does not stick to a wound surface.

The invention relates to a preparation method for a conduction and sustained release type nervous tissue engineering scaffold. The method comprises the following steps: silk fibroin Silk and lactic acid-caprolactone copolymer are dissolved in a solvent, dissolved and stirred to obtain a solution, then polyaniline and camphorsulfonic acid are added and stirred and mixed uniformly to obtain a cortex electrospinning solution, and NGF (nerve growth factors) are dissolved in ultrapure water completely to obtain a core electrospinning solution; and the cortex electrospinning solution and the core electrospinning solution are accommodated in an injector respectively for coaxial electrospinning, and then fumigation treatment and vacuum drying are performed to obtain the conduction type nervous tissue engineering scaffold. According to the prepared nanofiber scaffold, the nerve repair speed is increased from ways including external electrical stimulation (conductive polymer), biochemistry (NGFs), a topological structure required by nerve regeneration (orientation guide) and the like. The method is simple to operate, good in repeatability and high in economic benefit, and provides novel experimental thought for nerve defect repair in clinical application.

The invention relates to a cytomedicine for preventing intrauterine adhesion and repairing endometrium injury, and a preparation method thereof. The cytomedicine consists of umbilical cord or placenta mesenchymal stem cells, recombinant human stromal cell-derived factor-1alpha (rhSDF-1alpha), and sodium hyaluronate gel which is used for bearing the umbilical cord or placenta mesenchymal stem cells and the rhSDF-1alpha and has sustained-release and anti-inflammatory effects. When the cytomedicine is injected into a uterine cavity, the curative effects of promoting stem cells and uterus tissue cells to migrate to the injured part, reducing intimal tissue fibrosis, promoting endometrial regeneration and repair, physically obstructing and preventing intrauterine adhesion and the like are achieved. The problems that the stem cells are singly applied to be difficult in local position as well as the traditional intrauterine adhesion and endometrium injury repair method cannot realize serious endometrium injury repair or is short in action time and poor in local curative effect are solved. The preparation provided by the invention is simple in preparation method, easy to implement, safe and effective, and can doubly improve the concentration of the stem cells in the injured part, prolong the action time of the stem cells and improve local curative effect.

The invention discloses the preparation and clinical application of a fat-derived stromal cell composite graft material rich in cytokines. The preparation method is through 1) the acquisition and purification of fat granules; 2) the acquisition of nano-fat stromal cells; 3) the biological characteristics and multi-directional differentiation identification of nano-fat-derived stem cells (NFSCs); 4) fibrin glue rich in cytokines The preparation of transplant materials in four steps is used for the clinical repair and correction of sunken deformities of the face, chest and other body surface soft tissues. The invention is based on the new technology of stromal vascular components to promote trauma or wound repair, reduce scar hyperplasia, promote tissue regeneration repair and organ reconstruction, shorten the recovery time of patients, improve the quality of rehabilitation, and achieve good curative effect. It is a clinically assisted autologous fat Tissue transplantation provides experimental basis and theoretical support for the repair of soft tissue or organ defects, burn wounds, refractory wounds, and breast reconstruction after breast cancer surgery.

The invention relates to a hydrocolloid oil yarn. The hydrocolloid oil yarn comprises a base material and a hydrocolloid ingredient, wherein the base material is netty polyester fiber; the hydrocolloid ingredient comprises the following raw materials in parts by weight: 8 to 10 parts of thermoplastic elastomers, 16 to 30 parts of sodium carboxymethylcellulose, 1 to 5 parts of growth factor microspheres, 1 to 5 parts of chitosan, 100 to 200 parts of liquid paraffin, 30 to 40 parts of Vaseline and 5 to 10 parts of wool fat. The content of grown factors inside the grown factor microspheres is 0.01wt% to 0.03wt%.

The invention provides a zinc-containing artificial bone for bone tissue repair and a preparation method thereof. The zinc-containing artificial bone is prepared from the following raw materials in percentage by mass: 0.1-15% of zinc-containing powder, 80-98% of bioceramic powder and 0.5-10% of high-molecular polymer adhesive. The preparation method of the zinc-containing artificial bone comprisesa 3D printing technique. The method solves the problems of poor bone inductivity and poor bone formation promoting property in the traditional bioceramic artificial bone, and solves the problems of poor mechanical strength and difficulty in satisfying the bone repair demands of the bearing part in the traditional bioceramic artificial bone.

The invention discloses a biomimetic hydroxylapatite powder/gelatin/sodium alginate composite 3D print bracket and a preparation method thereof. The preparation method of the biomimetic 3D bracket comprises the steps of (1) preparing hydroxylapatite powder having a biomimetic classified structure; (2) preparing hydroxylapatite powder/gelatin/sodium alginate composite pulp; and (3) preparing the biomimetic 3D print bracket. According to the biomimetic hydroxylapatite powder/gelatin/sodium alginate composite 3D print bracket and the preparation method thereof disclosed by the invention, a 3D printing technique is utilized, the prepared organic/inorganic composite three-dimensional print bracket is controllable in outside size and good in biocompatibility, components and a multi-level structure of natural bone tissue are sufficiently simulated, and controlled mechanical strength is given to materials; and the micro-nano multi-level structure of the composite bracket has higher osteogenesis activity and osteoinductive, a multi-level pore structure can effectively induce ingrowth of tissue and blood vessels, and regeneration and repair of the bone tissue are promoted.

The invention provides a natural plant extract together with a preparation method and application thereof. The plant extract is prepared by being extracted from raw materials of grape skins, hawthorn fruits, seabackthorn fruits, barbary wolfberry fruits, lotus leaves, semen coicis, rhizoma polygonati, roots of thomson kudzuvine, sharpleaf galangal fruits, etc. with menstruum and finely processed. The preparation method can be divided into two kinds, a single extraction and a mixed extraction. The natural plant extract can effectively control the caloric intake absorption of organisms, reduce fat accumulation in the body and harm of over nutrition to organisms, decrease blood fat, purify blood, control blood sugar and interdict and reverse the development towards sub-health state, thus reducing the morbidity risk of diseases, such as hyperlipemia, hypertension, diabetes mellitus, coronary heart disease, adiposity, fatty liver, etc.; the natural plant extract is edible for healthy population to maintain health and for sub-health population to effectively interdict and reverse the development towards sub-health state and can also be applied to the preparation of the medicine treating hypertension, hyperlipemia, diabetes mellitus and other diseases through deep processing.

The invention provides a natural plant extract, a preparation method and the application. The invention is characterized in that: the natural plant extract is prepared by grape skin, haw apple, lotus leaf, medlar, sea buckthorn, fiveleaf gynostemma herb, pilose asiabell root, radix polygoni multiflori preparata and other materials, and the materials are processed solvent extract, machining and refining and then the natural plant extract is obtained; the preparation method comprises A individual extraction method and B mixed extraction method. The natural plant extract has the advantages of effectively controlling energy absorbing of bodies, reducing fat accumulation in vivo and reducing harm of overnutrition to bodies, reducing blood fat, purifying blood, controlling blood sugar, interdicting and reversing the development of sub-healthy state, reducing morbidity risk of hyperlipemia, hypertension, diabetes, coronary heart disease, fatness, fatty liver and other diseases, providing healthy population for eating to keep fit, also providing sub-healthy population for eating to effectively interdict and reverse the development of sub-healthy state, still being used to prepare the medicament for curing hypertension, hyperlipemia, diabetes and other diseases after deep processing.

The present invention relates to a skin regenerating repair and anti-aging mask for electromagnetic radiation and fog-haze pollution, and a preparation method thereof, and belongs to the field of skin care products. According to the present invention, the mask cloth is formed by weaving charcoal fibers and plant wood pulp, and the mask solution essence comprises the following raw materials 8-20% of a ganoderma lucidum extract, 4-10% of a human placenta extract, 1-3% of acetyl hexapeptide-2, 1-3% of a silphium perfoliatum extract SOD, 1-3% of tocopherol, 1-3% of tranexamic acid, 3-5% of a snail mucilage extract, 0.05-2% of a hamamelis virginiana extract, 0.3% of epidermis cell growth factor (EGF), 0.05-3% of sodium hyaluronate, 0.3% of alpha-arbutin, 0.01% of EDTA tetrasodium, 0.2% of triethanolamine, 11% of butanediol, and the balance of deionized water. According to the present invention, the mask has effects of deep absorption and removal of all dirt on skin, oxygen free radical scavenging, resistance of harm of electromagnetic radiation and fog-haze pollution on skin, moist, silky, delicate, uniform and clean skin state achieving, and young state achieving.

The invention relates to the technical field of wound dressings, in particular to a tissue-specific wound dressing as well as a preparation method and application thereof. The tissue-specific wound dressing comprises an extracellular matrix extracted from tissue and a gelling material, wherein the tissue is an organ or an animal organ or tissue corresponding to the organ or tissue to which the dressing is applied, and the gelling material can crosslink and cure the extracellular matrix, so that the extracellular matrix has high adhesion to wet tissue and can be quickly adhered to a wound to stop bleeding. According to the tissue-specific wound dressing, the extracellular matrix and the gelling material are matched, so that compared with a common wound dressing, the tissue-specific wound dressing can shorten wound healing and tissue repairing time remarkably, has good hemostatic and wound healing effects, and can be used for massive hemorrhage or visceral hemorrhage. The tissue-specificwound dressing fills the blank that the extracellular matrix is used as a wound dressing.

The invention discloses a tissue engineering meniscus composite scaffold and a preparation method thereof. The scaffold comprises: a C-shape bracket which is consistent with the initial shape of meniscus to be regenerated and repaired, the bracket comprising a plurality of first degradable polymer material fibers extending in a circumferential direction of the bracket and a plurality of second degradable polymer material fibers extending in a radial direction of the bracket, the first degradable polymer material fiber and the second degradable polymer material fiber being arranged in a multilayer cross arrangement to form a frame structure having a plurality of first pores; a matrix material composited within a plurality of first apertures of the stent to form a meniscus composite stent having a plurality of second apertures. The meniscus composite scaffold provided by the invention has excellent mechanical properties and biocompatibility, and can provide an excellent microenvironmentrequired for cell growth, thereby enabling the newborn meniscus to have excellent shape, structure, mechanical properties and physiological functions.

The invention discloses a growth factor sustained release microsphere, a tissue engineering cartilage composite stent and a preparation method. The growth factor sustained release microsphere includesa polylactic acid-glycolic acid copolymer matrix material and recombinant human transforming growth factor beta 3 encapsulated in the matrix material, wherein the encapsulation amount of the recombinant human transforming growth factor beta 3 in the microsphere is 5 ng/mg to 200 ng/mg; the sustained release period of the microsphere is 28 days or above. The growth factor sustained release microsphere and the tissue engineering cartilage composite stent disclosed by the invention have higher regeneration and repair effects of defected cartilage tissues.

The invention discloses a composite antibacterial aerosol as well as a preparation method and application thereof and belongs to the technical field of antibacterial agents. The composite antibacterial aerosol disclosed by the invention contains the following raw materials in percentage by weight: 0.5-3.0% of chitosan biquaternary ammonium salt, 0.05-0.3% of nano titanium dioxide, 0.9-2.2% of a sea salt and the balance of purified water. The invention further discloses a preparation method of the aerosol. The preparation method comprises the following steps: uniformly mixing the chitosan biquaternary ammonium salt, the nano titanium dioxide and the sea salt, adding the purified watrer, and enabling the materials to be fully dissolved, thereby obtaining the aerosol. The aerosol can be applied to cleaning of the nasal cavity and oral cavity and postoperative cleaning. The aerosol disclosed by the invention is simple in formula, low in cost, excellent in biocompatibility, simple in preparation method, obvious in antibacterial and bacteriostatic effects, safe and non-irritating, and achieves the effects of relieving inflammations of the nasal cavity and oral cavity, keeping the nasal mucosa and oral cavity wet, eliminating postoperative peripheral inflammation, preventing and controlling wound infection, promoting regeneration and repair of the mucous membranes and tissues and promoting wound healing.

The invention belongs to the field of biotechnology and provides a stem cell induced culture solution used for eyes .The stem cell induced culture solution comprises a vascular endothelial cell growth factor (VEGF), a basic fibroblast growth factor (bFGF) and an epidermal growth factor (EGF); the content of the vascular endothelial cell growth factor (VEGF) is 0.5-2.5 ng/ml, the content of the basic fibroblast growth factor (bFGF) is 0.25-0.4 ng/ml, and the content of the epidermal growth factor (EGF) is 0.5-2.0 ng/ml .The stem cell (ASCs) in vitro conditioned culture medium from human eyepit fat is adopted, and a significant reinforcement function is achieved for regeneration and repair of corneal injury.

The present invention relates to one kind of compound triptolide alcohol preparation for being injected into articular cavity to treat osteoarthritis. It includes triptolide alcohol solution of 1*10<-4> to 5*10<-2> mg/ml concentration and gel liquid mixed in the volume ratio of 1 to 1-9. It has medicine slow releasing effect, and this can prolong the action period of medicine, reduce consumption of triptolide alcohol and lower the toxic side effect of the medicine. It can inhibit the further development of osteoarthritis in early and middle phase, improve the environment inside the joint and promote the regeneration and repair of articular cartilage to reach the aim of cure osteoarthritis.

The invention discloses a composition based on stem cells and application thereof in preparing preparation for the coronary heart disease. The composition based on the stem cells includes the stem cells prepared through an optimizing method and cell preserving liquid serving as a solvent. Firstly, proper stem cells are selected; secondly, the selected stem cells are further processed through the optimizing method; lastly, the stem cells are collected and resuspended in the cell preserving liquid. A various cell implanting method is adopted in the application of the composition based on the stem cells in preparing the preparation for the coronary heart disease, the time nodes before an operation, during the operation and after the operation are combined, and it is ensured that the transplanted stem cells can give the full play. The optimizing method is used for preparing the stem cells and the stem cell composition in the cell preserving liquid, the ventricle function can be extremely obviously enhanced after cell transplantation, and the left ventricle reconstitution is restrained. The optimal cell composition is provided for treating the coronary heart disease, the source is wide, preparation is simple, toxic and side effects are avoided, and the transplanting effect is good.

The invention discloses a method for manufacturing a magnesium phosphate bioceramic functional gradient bone regeneration scaffold. The method comprises the following steps: (1) preparing MgP powder;(2) preparing a MgP scaffold mixed solution; (3) preparing a HIPS porous support scaffold through 3D printing; and (4) preparing a blank MgP scaffold so as to obtain the MgP functional gradient bone regeneration scaffold. The scaffold material has a multi-scale pore structure, the inner layer has loose pores, and the pores are 300-500 [mu]m, not only can be beneficial to cell growth, but also canbe rapidly degraded and promote the formation of a natural bone structure; the outer layer scaffold is provided with dense pores, and the pores are 100-200 [mu]m, provide enough mechanical strength for the scaffold, provide longer degradation time, provide a stable regeneration microenvironment for internal bone regeneration, and have the effect of promoting bone defect regeneration and repair; and meanwhile, the surface of the scaffold material is also provided with micropores which are about 50 [mu]m and are beneficial to the adhesion of cells.

The invention provides a polypeptide repairing emulsion and a preparing method thereof. Every 100 g of the polypeptide repairing emulsion is prepared from 3 g-5 g of propylene glycol, 0.3 g-0.5 g of xanthan gum, 0.1 g-0.2 g of allantoin, 0.1 g-0.2 g of polyacrylic acid, 0.1 g-0.2 g of sodium hyaluronate, 1 g-3 g of acetyl hexapeptide-8, 1 g-3 g of palmitoyl tripeptide-1, 1 g-3 g of inositol, 1 g-3g of tripeptide-1, 1 g-3 g of copper peptide, 1 g-3 g of acetyl tetrapeptide-5, 1 g-3 g of creatine, 1 g-3 g of palmitoyl tetrapeptide-7, 1 g-3 g of carnosine, 0.1 g-0.3 g of methylparaben, 1 g-3 g of polysorbate-20, 0.5 g-0.8 g of panthenol, 0.05 g-0.1 g of EDTA disodium, 0.2 g-0.8 g of phenoxyethanol, 0.1 g-0.3 g of bi-(hydroxymethyl) imidazolidinyl urea, 0.1 g-0.3 g of iodopropynyl butylcarbamate and the balance deionized water. The invention also provides a method for preparing the polypeptide repairing emulsion. The polypeptide repairing emulsion has the advantages shown in the description.

The invention discloses a pleura/meninges patch and a preparation method thereof. The preparation method comprises the following steps: S1, dissolving squid cartilage chitosan to form a glue solution, wherein the deacetylation degree of the squid cartilage chitosan is greater than 60% and the molecular weight of the squid cartilage chitosan is greater than 0.5*10<6>; and S2, coating the glue solution to obtain the pleura/meninges patch. The pleura/meninges patch prepared by the method is capable of effectively promoting regeneration and rehabilitation of pleura and endocranium of a human body, is finally degraded and absorbed in vivo, and has relatively high clinical application value.

The invention provides a nerve film and a preparation method thereof, and relates to the field of biomedicine. The preparation method of the nerve film comprises: mixing polycaprolactone with an isocyanate substance for a reaction, and then adding a chain extender for a reaction, so as to obtain a polyurethane prepolymer; mixing the polyurethane prepolymer with gastrodin for a reaction, so as to obtain a first preliminary product, mixing the first preliminary product with water for a reaction, so as to obtain a second preliminary product, curing the second preliminary product and conducting drying to obtain a polyurethane nerve material; dissolving the polyurethane nerve material by an organic solvent, reacting the dissolved polyurethane nerve material with a pore-forming agent to obtain an emulsion initial product, conducting performing and air drying on the emulsion initial product, and dissolving the pore-forming agent by water under a vacuum condition, so as to obtain the nerve film. The nerve film is a porous structure through which pores penetrate, and the porous structure is favorable for cell adhesion and infiltration. The nerve film has good tortuosity, folding propertiesand biocompatibility. Slowly released gastrodin of the material is beneficial to cell proliferation and up-regulation of neurotrophic factors, and promotes elongation of axons.

The invention discloses an implantable bandage for promoting bone injury repair and a preparation method thereof. The bandage is composed of a porous grid sheet-shaped object and organic-inorganic composite superfine fiber porous film covering objects on the surfaces of the two sides of the porous grid sheet-shaped object, the lower layer is a superfine fiber porous network containing bioglass particles, the middle layer is a polymer porous grid, and the upper layer is a superfine fiber porous network containing inorganic mineral particles; inorganic matters in the superfine fibers on the surfaces of the two sides are bioglass particles for inhibiting inflammation, resisting infection and promoting soft tissue regeneration and repair and inorganic mineral particles for inhibiting inflammation and promoting bone regeneration and repair, and the bandage is prepared through three-dimensional printing and electrostatic spinning processes. The method is convenient to operate, has a remarkable inhibition effect on inflammation in soft and hard tissues such as muscles, periosteum and bones with various fractures or bone defects, promotes rapid healing and repair of the soft and hard tissues, is adjustable in degradation rate of the implantable bandage, and has a remarkable bacteriostasis effect on common pathogenic bacteria and fungi.