Application of nano porous microbracket in tissue regeneration and repair

A nanoporous, tissue regeneration technology, applied in the field of regenerative medicine, can solve the problems of undeveloped stem cell survival, expansion, directed differentiation and injectable three-dimensional porous microscaffolds, etc., achieve small physical size, promote adherence, and facilitate growth the effect of entering

Active Publication Date: 2018-12-07
PEKING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, injectable three-dimensional porous microscaffolds that can effectively promote stem cell survival, expansion, and directed differentiation have not yet been developed.

Method used

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  • Application of nano porous microbracket in tissue regeneration and repair
  • Application of nano porous microbracket in tissue regeneration and repair
  • Application of nano porous microbracket in tissue regeneration and repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0124] Example 1 Preparation of nanocarrier-sodium alginate three-dimensional porous micro-scaffold

[0125] 1.1. Synthesis of peptide-laden mesoporous silica nanoparticles (pep@MSNs)

[0126] Mesoporous silica nanoparticles (MSNs) were obtained from Wei Jie's laboratory at East China University of Science and Technology. The specific preparation method is: add 3~9g of Pluronic P-123 (Sigma), 125~130mL of deionized water and 18~25mL of concentrated HCl into a round bottom flask, stir vigorously for 1.5~4h, mix well and place the flask at 50°C In a water bath, add 5-10 g of ethyl orthosilicate while stirring, and stir for 20 hours. Then raise the temperature to 80-85°C, and let it stand at constant temperature for 24-30 hours; then centrifuge (5000-6000G), wash the precipitate with deionized water, dry it and calcinate it in a muffle furnace.

[0127] In this study, the polypeptide we used was a polypeptide derived from the immature region of BMP-7, named BFP-1 (GQGFSYPYKAV...

Embodiment 2

[0139] Example 2 Characterization of nanocarrier-sodium alginate three-dimensional porous micro-scaffold

[0140] Scanning electron microscopy (SEM; S-4800; Hitachi, Japan) was used to characterize the microstructure (pore size, porous morphology, etc.) , USA) was used to determine the elastic modulus of each scaffold.

[0141] Soak the freeze-dried micro-scaffold in PBS, after it fully absorbs water and swells, filter off the PBS, add a little low-concentration sodium alginate solution to the micro-scaffold and mix well, then transfer to a syringe (1mL size) for freeze-drying . For in vivo experiments, just add the cell suspension to the micro-scaffold and mix well before injection. Injectability was verified with dense tissue.

[0142] The sustained-release properties of BFP-1 coated in NAC / MS were characterized by measuring the sustained-release profile of BFP-1 over time. Briefly, the BFP-1 used in the preparation of NAC / MS for the determination of sustained-release ...

Embodiment 3

[0150] Embodiment 3 injectable NAC / MS preparation

[0151] Soak the prepared NAC / MS in PBS at 4°C for 48h; then filter off the PBS, transfer the NAC / MS to a 1ml syringe, plug the injection plunger, freeze-dry and store in a sealed container at 4°C.

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Abstract

The invention discloses an application of a nano porous microbracket in tissue regeneration and repair. The nano porous microbracket is a nano carrier-sodium alginate three-dimensional porous microbracket; the nano carrier is mesoporous silicon dioxide nanoparticles; the sodium alginate is modified sodium alginate; and the nano carrier-sodium alginate three-dimensional porous microbracket is prepared by adopting a liquid nitrogen quick freezing high speed breaking method. The special application method of the nano carrier-sodium alginate three-dimensional porous microbracket in tissue regeneration and repair comprises the following steps: adding PBS which accounts for 50-80% of the total volume of the microbracket into a prepared microbracket to obtain an injectable microbracket; and injecting the injectable microbracket to a damaged tissue part. The nano porous microbracket can promote tissue regeneration and promotes cell amplification and oriented differentiation of stem cells, is injectable and is high in clinical practicality. The invention also discloses applications of the nano porous microbracket in promoting stem cell proliferation, stem cell differentiation and tissue self-regeneration and repair.

Description

technical field [0001] The invention relates to the application of a scaffold in tissue regeneration and repair, and belongs to the field of regenerative medicine, in particular to the application of a nanoporous micro-stent in tissue regeneration and repair. Background technique [0002] By simulating the physical and chemical properties of the natural microenvironment of the human body, biological scaffolds can effectively promote the self-renewal, proliferation, and directed differentiation of stem cells, and have been widely used in the field of regenerative medicine in recent years. [0003] Generally, stem cell-based bioscaffolds are mainly divided into porous scaffolds and hydrogel scaffolds according to their state. Among them, the porous scaffold has a large number of interconnected three-dimensional porous structures, which are more conducive to the migration of cells in the scaffold and the supply of nutrients compared to hydrogels. It is generally believed that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/02A61L27/50A61L27/54A61L27/56C12N5/00C12N11/10C12N11/14
CPCA61L27/025A61L27/20A61L27/50A61L27/54A61L27/56A61L2400/06C12N5/0075C12N11/10C12N11/14C12N2533/74C08L5/04
Inventor 魏世成罗祖源潘冀佳陈庆林
Owner PEKING UNIV
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