417results about "General culture methods" patented technology

Methods for differentiating pluripotent stem cells to neuroectoderm in dynamic suspension culture using small molecule or protein inhibitors of TGFβ / Activin / Nodal signaling and BMP signaling are provided. Also provided are methoc and protocols for differentiating pluripotent stem cells such as human embryonic stem cells first to neuroectoderm, then further to glial progenitor cells, and further to oligodendrocyte progenitor cells (OPCs), and compositions obtained thereby. The methods of the present disclosure reproducibly produce neuroectoderm progenitor cells by day 7 of the differentiation process, glial progenitor cells by day 21 of the differentiation process and OPCs by day 42 of the differentiation process.

Provided herein are peptide amphiphiles (PAs) comprising a bioactive peptide, nanofibers displaying the bioactive PAs, and methods of use thereof. The disclosed peptide amphiphiles comprise a hydrophobic tail, a structural peptide segment, a charged peptide segment, and a bioactive IKVAV peptide. The disclosed PAs may be used in cell culture methods and in methods of treating central nervous system injury.

A method of modifying a surface of a three-dimensional (3D) article includes immersing at least one part of the 3D article in a buffered solution of functionalized peptides, allowing reaction between the functionalized peptides and reactive groups on the surface of the 3D article; and washing the at least one part of the 3D article to remove unreacted functionalized peptides. The surface-modified 3D article includes a plurality of peptides covalently bonded to the surface of the 3D article via a cysteine bridge. The surface- modified 3D article can be used as a scaffold for the formation of biological tissue or bodily implants.

The present invention provides constructs including a tubular biodegradable polyglycolic acid scaffold, wherein the scaffold may be coated with extracellular matrix proteins and substantially acellular. The constructs can be utilized as an arteriovenous graft, a coronary graft, a peripheral artery bypass conduit, or a urinary conduit. The present invention also provides methods of producing such constructs.

The present disclosure relates to a transplantable cell therapy product composition for diabetes mellitus that contains an aggregate of insulin-secreting cells derived from stem cells. In the present disclosure, an NF microwell array membrane was fabricated by applying a molding process to an electrospun, permeable, biodegradable polycaprolactone (PCL) NF membrane and thus allows gases and soluble factors to permeate therethrough. The NF microwell of the present disclosure could provide more nutrients to the iPSC aggregates than conventional impermeable PDMS microwells, thus enhancing survival and differentiation capabilities of the cells. Additionally, the NF membrane was attached singly to the subcutaneous tissue and to the surface of organs such as liver and peritoneum without the need for a fixing material or separate sutures and was integrated with surrounding tissues, resulting in higher insulin secretion than PDMS microwells. Therefore, the present disclosure can be effectively utilized as a composition for the prevention or treatment of diabetes.

A cell culture microcarrier bead is proposed. The microcarrier bead comprises a bead body having its surface flecked with plasmonic nanoparticles. In a second aspect, the invention relates to a cell culture reactor, containing a cell culture medium and the proposed microcarrier beads. A third aspect of the invention concerns a method for observing living cells on such microcarrier beads. Yet a further aspect of the invention relates to a method for packing nanoparticles on a carrier body.

The invention provides a method for preparing an ordered cell-containing microarray, and a system for preparing an ordered cell-containing microarray.

The application pertains to compositions and methods useful for growing living cells such as stem cells. The compositions employ a mixture of an extracellular matrix and discrete carbon nanotubes. The extracellular matrix may also comprise components selected from the class of proteins, proteoglycans, polysaccharides, lipids, peptides, messenger molecules, signaling molecules, or any mixture thereof. The discrete carbon nanotubes are usually less than about 1% by weight of the dry weight of the total composition.

The invention relates to a polylactic acid microcarrier and a method for cell amplification by using the polylactic acid microcarrier. The preparation method disclosed by the invention is simple and convenient to operate, the prepared polylactic acid microcarrier is uniform in particle size and pore size distribution, the microcarrier is subjected to surface retreatment, more active sites are provided for cell adhesion, a three-dimensional matrix environment closer to a body is provided, cell amplification is facilitated, the dryness and differentiation potential of stem cells are maintained, and the preparation method is suitable for large-scale popularization and application. And the microcarrier can be repeatedly used, so that the use cost is reduced.

The present invention relates to a polymer conjugate comprising a ULA polymer a) and one or more polypeptides b), wherein the polypeptides are conjugated to the ULA polymer, and the conjugate is coated on the surface of a device for culturing cells. The peptides can interact with receptors on the surface of the cells, while the polymer prevents non-specific binding of the cells to the surface of the device.

Provided herein are compositions comprising a particle comprising a library encoded bead (e.g., DEL bead) coated in a polymeric matrix forming a core-shell particle, wherein the core-shell particle is further inside a 3D tissue culture. Also provided herein are methods of making such particles, and methods of using such particles for drug discovery such as identification of bioactive compounds via high-throughput cellular activity-based phenotypic screens of DNA-encoded chemical library beads.

The present invention relates to novel cell culture and transfection methods using a wildtype Caf1 ("Caf1-WT") polymer or a variant thereof. Use of a Caf1-WT polymer or a variant thereof to increase cell viability, cell proliferation, cell migration and / or cell clustering; promote the formation of a 3D cell structure; and / or promote suspension cell culture of cells that are normally adherent are also provided herein.

The present disclose relates to a high-reactivity hydrogel particle and preparation method therefor and use thereof. The preparation method comprises the following steps: (1) performing polymerization reaction of double-bond modified polyethylene glycol, a modified natural polymer and an optional active functional monomer to obtain a gel; (2) smashing the gel to obtain a gel particle; and (3) performing enzymolysis reaction of the gel particle and an enzyme to obtain the hydrogel particle with reactivity. The double-bond modified polyethylene glycol has a double bond and a polyethylene glycol chain segment, the modified natural polymer has sulfydryl or a double bond, the active functional monomer is selected from a sulfydryl functionalized biological adhesive peptide or sulfydryl functionalized cholesterol, and the enzyme is an enzyme corresponding to the natural polymer. The obtained hydrogel particle has high reactivity and can be used for subsequent secondary crosslinking. The hydrogel particle also has good biocompatibility.

The present disclosure relates to a method for producing three-dimensional cell clusters on an inorganic cell culture platform comprising a three-dimensional structure, preferably a fractal structure. Such three-dimensional structures are preferably useful for culturing cells and tissues in three dimensions. Such three-dimensional structures are preferably useful for inducing differentiation of non-embryonic stem cells. In particular, such three-dimensional (3D) structures are useful for culturing primary tissue cells.

Provided are a well plate and a culture method which are excellent in ability to attach cells, etc., and exhibit a low drug adsorption property. The well plate including a bottom surface component and a well lateral wall component, wherein the bottom surface component and the well lateral wall component are bonded to each other to form at least one well, the bottom surface component and the well lateral wall component are formed of different materials, and a surface free energy of the well lateral wall component is 18.0 to 34.0 mN / m.

The present invention relates to a tumor tissue model in which tumor tissue isolated from a patient can be cultured. The tumor tissue model uses tumor tissue isolated from a patient, and thus the tumor tissue can be cultured while maintaining the extracellular matrix of tumor cells. Since the tumor tissue is cultured in an environment similar to the actual environment inside the body, the tumor tissue model can be used for patient-tailored drug screening, drug response evaluation, radiation reactivity evaluation, tumor-related research, clinical evaluation, animal replacement tests, disease modeling, etc.

The present invention relates to a low-serum medium composition for culturing Vero cells, and a method for culturing Vero cells and a method for producing a virus, both using the same.

The present disclosure relates to a cultured meat scaffold, a method of preparing the same, and cultured meat including the same. The cultured meat scaffold according to the present disclosure includes a nanocellulose; and a polymer hydrogel.

The presently disclosed subject matter provides an approach to address the needs for microscale control in shaping the spacial geometry and microarchitecture of 3D collagen hydrogels. For example, the disclosed subject matter provides for compositions, methods, and systems employing N-sulfosuccinimidyl-6-(4′-azido-2′-nitro-phenylamino)hexanoate (“sulfo-SANPAH”), to prevent detachment of the hydrogel from the anchoring substrate due to cell-mediated contraction.

The present disclosure relates to a method for producing leather, comprising culturing cells on a honeycomb macroporous polymer scaffold. The present disclosure also relates to leather obtained by this method.

The invention discloses a microcarrier with the surface modified with a herpes virus receptor and application of the microcarrier, and relates to the technical field of animal virology technologies and veterinary biological products. The microcarrier is composed of a microcarrier matrix and herpes virus specific receptor protein fixed to the surface of the microcarrier matrix through chemical coupling; the microcarrier matrix is made of a biocompatible material, and the particle size of the microcarrier matrix is 1-10 [mu] m; the herpes virus specific receptor protein is an extracellular region fragment of a nectin-1 receptor. The method has the advantages that the virus infection efficiency and the virus yield are remarkably improved by accurately regulating and controlling the particle size of the microcarrier and adopting a receptor fixing technology, and the performance is particularly excellent under the condition of low infection complex number; meanwhile, the process is greatly simplified, and the pollution risk is reduced; and the method can be widely applied to herpes virus production, vaccine preparation and difficult-to-culture virus amplification by replacing receptors, and has a wide application prospect.

A method for screening target cells, a corresponding test kit and its use, a method for screening cells, and a biological culture chip and its preparation method and its use. A method for screening target cells includes culturing the candidate single cells in a culture chamber equipped with a signal screening layer, the signal screening layer containing a signaling molecule for specifically recognizing the target molecule; and selecting target cells suitable for secreting the target molecule based on the signal of the signaling molecule. The cell screening method includes placing candidate cells in a culture chamber to form a target antibody-antigen signaling antibody compound; and determining whether the candidate cells are target cells based on the signal of the signaling molecule bound to the signaling antibody. The biological culture chip includes a matrix (100) and a biological culture space disposed on the surface of the matrix (100) and used for culturing biological cells.

Provided herein are modified gelatins, cross-linked modified gelatins and hydrogels, and processes for making the same. Also provided herein is the use of such materials as matrices for cell growth.

The present invention relates to a method for the preparation of an assembly of cells and microrobots comprising (a) preparing a suspension of cells and microrobots, wherein the microrobots comprises an alginate, collagen, fibrin, hyaluronic acid / collagen, polyacrylamide, or polyethylene glycol hydrogel capsule, wherein the alginate is conjugated to cell adhesive peptides, and (b) incubating the suspension of (a) to allow the microrobots to form an assembly with the cells via the binding of the cell adhesive peptides to the cells, wherein step (b) and preferably steps (a) and (b) are performed in a conic microcentrifuge tube with a rounded bottom without corners and edges.

We provide cultured cell sheets. [Solution] A cultured cell sheet is provided, which is made from cells collected from nasal mucosal tissue, and contains 50-90% undifferentiated cells relative to the total number of cells, wherein, in one embodiment, the undifferentiated cells are positive for p63, and the cultured cell sheet is a mixture of nasal mucosal epithelial cells and nasal mucosal epithelial stem cells or nasal mucosal epithelial progenitor cells, as well as one or more of the following cells: other epithelial cells or other epithelial stem cells, mesenchymal stem cells, fibroblasts, vascular endothelial cells, vascular endothelial progenitor cells, and adipocytes. [Effects] The cultured cell sheet of the present invention engrafts on areas of mucosal tissue defects such as inflammation and granulation tissue formation on the surface of bone tissue in the middle ear, promoting the regeneration of mucosal tissue, including mucosal tissue covering bone tissue, efficiently suppressing inflammation occurring in the bone tissue of the middle ear, and inhibiting characteristic fibrosis, granulation tissue formation, and poor epithelial formation that occur during bone tissue inflammation.

The present disclosure provides, inter alia, a bilayer semipermeable fibrosis-resistant hydrogel polymer enclosure optionally containing cells such as mammalian cells, the enclosure being suitable for administration to a subject in need thereof, and further, the enclosure being expandable in accordance with a large number of delivered cells. The present disclosure further provides methods of making and using the enclosure, for example, in a treatment method, for example, in the treatment of disorders of the liver or other cells and organs treatable with secreting cells and / or catalytic cells.

A bio-ink comprising: (a) at least one polysaccharide; (b) at least one structural protein; and (c) fibrinogen, wherein the polysaccharide is selected from a group consisting of Sodium alginate Chitosan, Hyaluronic acid, Gellan gum, Dextran, Agarose, Poly(ethylene glycol), Pluronic and Carrageenan and the structural protein is selected from a group consisting of Gelatin, Collagen Vitronectin, Laminins and Fibronectin.

To provide a resin film formed of a scaffold material for cell culture, the resin film enabling excellent fixation of cells after seeding and thus contributing to an increase in the cell proliferation rate.SOLUTION: A resin film is formed of a scaffold material for cell culture, wherein the scaffold material for cell culture comprises a synthetic resin with a polyvinyl acetal skeleton; the resin film has a phase-separation structure containing at least a first phase and a second phase; and the ratio of the surface area of one of the first and second phases to the total surface area is 0.01-0.95 inclusive.SELECTED DRAWING: Figure 1

To provide a biological culture chip suitable even for complex detection of cell biological functions such as antibody secretion and growth factor secretion.SOLUTION: Provided is a biological culture chip including a matrix formed of a matrix material, and culture chambers formed on a surface of the matrix to define the culture chambers, the matrix material of the matrix having an equilibrium swelling ratio of 1.25 to 1.75 and the culture chambers being formed in openings configured on the surface of the matrix to define the culture chambers.SELECTED DRAWING: Figure 3

The invention belongs to the field of biomedical engineering, and particularly relates to an amino acid modified molybdenum disulfide artificial extracellular matrix and application. Preparing an amino acid modified molybdenum disulfide artificial extracellular matrix, inoculating the neuron in-vitro model PC12 cell suspension onto the artificial extracellular matrix, and constructing the nerve cell in-vitro differentiation model. The artificial extracellular matrix with good biocompatibility is obtained by constructing different kinds of amino acid modified molybdenum disulfide nano material artificial extracellular matrixes as the artificial extracellular matrix of nerve cells PC12, and the artificial extracellular matrix improves the activity of the PC12 cells and promotes the differentiation of nerve cell synapses; a neuron in-vitro model with high differentiation and higher activity is constructed, and the artificial extracellular matrix can be used for nerve regeneration and neural engineering.