The invention discloses scaffolds of
umbilical cord decellularized Wharton jelly for
tissue engineering and a preparation method thereof. Umbilical cords are employed as the
raw material and their outer membranes and vascular tissues are peeled off. And the rest part of the umbilical cords is subjected to hypotonic freeze-thaw, mechanical pulverization,
differential centrifugation,
enzymatic digestion for
decellularization. Then the
umbilical cord Wharton jelly is collected and injected into a mold. After
freeze drying and crosslinking, multiple three dimensional porous
sponge scaffolds and composite scaffolds can be obtained. The method of the invention has the advantages of wide material source, low cost, simple technology. And the prepared scaffolds are characterized by controllable
fine structure, appropriate degradation rate, good
biocompatibility, and
biomechanical strength, which are in favor of
cell adhesion and the uniform distribution of seed cells within the scaffolds, as well as seed
cell multiplication, migration and growth. Thus, the scaffolds of
umbilical cord decellularized Wharton jelly in the invention can be widely applied in the
tissue engineering field such ascartilage, bone,
skin and nerve, with a favorable clinical application prospect.