68 results about "Biomechanical strength" patented technology

The present invention relates to collagenous membranes produced from amnion, herein referred to as a collagen biofabric. The collagen biofabric of the invention has the structural integrity of the native non-treated amniotic membrane, i.e., the native tertiary and quaternary structure. The present invention provides a method for preparing a collagen biofabric from a placental membrane, preferably a human placental membrane having a chorionic and amniotic membrane, by decellularizing the amniotic membrane. In a preferred embodiment, the amniotic membrane is completely decellularized. The collagen biofabric of the invention has numerous utilities in the medical and surgical field including for example, blood vessel repair, construction and replacement of a blood vessel, tendon and ligament replacement, wound-dressing, surgical grafts, ophthalmic uses, sutures, and others. The benefits of the biofabric are, in part, due to its physical properties such as biomechanical strength, flexibility, suturability, and low immunogenicity, particularly when derived from human placenta.

Heat is generated in corneal fibrils in a cornea of an eye according to a selected pattern. The heat causes the corneal fibrils corresponding to the selected pattern to transition from a first structure to a second structure. The second structure provides a desired reshaping of the cornea. A cross-linking agent is then activated in the region of corneal fibrils according to the selected pattern. The cross-linking agent prevents the corneal fibrils from changing from the second structure. Thus, embodiments stabilize corneal tissue and improve its biomechanical strength after desired structural changes have been achieved in the corneal tissue. Accordingly, the embodiments help to preserve the desired reshaping of the cornea.

A hydraulic cement for biomedical applications. The cement sets in-situ, hardening when exposed to water to produce nano-dispersed composite of calcium-silicate-hydrate gel mixed with hydroxyapatite. In comparison with prior cements, the composition provides high biocompatibility, high bioactivity and high biomechanical strength, due to the composite structure of the calcium silicate hydrate reinforced with co-precipitated particles of hydroxyapatite. Biocompatibility is also increased due to an absence of aluminum and magnesium in the composition. The cement is suitable for variety of applications, including dental implants, bone fixation, and bone repair.

The invention discloses a three-dimensional porous cartilage extracellular matrix sponge scaffold made of natural cartilage, which can compound cells further to construct tissue engineered cartilage, and can be used for clinically repairing cartilage defects. In the invention, conditions which can fully perform cell extraction and form a porous scaffold matrix are provided by processing cartilage into cartilage microfilaments, and then the cell extraction and solidification and/or strengthening treatment are performed to obtain the three-dimensional porous cartilage extracellular matrix sponge scaffold which is completely decellurized. The antigenicity and cell components are removed in the natural cartilage, and an extracellular matrix component of the cartilage is retained to obtain the scaffold with appropriate pore diameter and porosity, suitable degradation rate, good biocompatibility and certain biomechanical strength. The scaffold has the advantages of broad material sources, low cost, simple and feasible preparation technology, and good repetitiveness; and the scaffold can be widely applied in the field of tissue engineering and has good clinical application prospect.

Biologic partial menisci (biologic partial meniscal replacements/constructs) used to replace at least a part of a meniscus, and methods of forming such biologic partial menisci. Meniscal cartilage is employed to form a moldable allograft paste. A sterile mold that replicates a meniscus (for example, the medial or lateral meniscus) is provided in various sizes and is used as a biologic mold to recreate the anatomic shape of the meniscus. The moldable paste is inserted (for example, injected) into the mold and allowed to set into a stable, anatomically shaped meniscus (the biologic partial meniscus). Fibrin glue or other biologic adhesives or strengtheners may be optionally added to provide further biomechanical strength. Once the biologic partial meniscus is removed from the mold, it is provided at the surgical site and attached to the excised meniscus (placed into the correct anatomical shape) to complete the meniscal repair.

The invention discloses an injectable composite bone cement of hydroxyapatite-PMMA containing strontium, consisting of a powder and a liquid monomer with the weight ratio of 1.5 to 2.5:1, wherein the main components of the powder are 10 to 30% of hydroxyapatite containing strontium and 70 to 90% of PMMA and/or PMMA/styrene copolymer in percentage by weight, and the main components of the liquid monomer are 98 to 99.9% of MMA and 0.1 to 2% of DMPT in percentage by weight. The bone cement has the advantages of safety, no toxity, convenient operation and molding, and low polymerization temperature. The bone cement has good biomechanics intensity, biological activity and biocompatibility, and can promote the growth of the bone, suppress the bone resorption and be used in radiological imaging because of containing the metallic element of strontium.

The invention discloses scaffolds of umbilical cord decellularized Wharton jelly for tissue engineering and a preparation method thereof. Umbilical cords are employed as the raw material and their outer membranes and vascular tissues are peeled off. And the rest part of the umbilical cords is subjected to hypotonic freeze-thaw, mechanical pulverization, differential centrifugation, enzymatic digestion for decellularization. Then the umbilical cord Wharton jelly is collected and injected into a mold. After freeze drying and crosslinking, multiple three dimensional porous sponge scaffolds and composite scaffolds can be obtained. The method of the invention has the advantages of wide material source, low cost, simple technology. And the prepared scaffolds are characterized by controllable fine structure, appropriate degradation rate, good biocompatibility, and biomechanical strength, which are in favor of cell adhesion and the uniform distribution of seed cells within the scaffolds, as well as seed cell multiplication, migration and growth. Thus, the scaffolds of umbilical cord decellularized Wharton jelly in the invention can be widely applied in the tissue engineering field such ascartilage, bone, skin and nerve, with a favorable clinical application prospect.

The invention discloses a preparation method of a natural-tissue-derived decellularized and decalcified bone material. According to the method, any bone tissue of a mammal is treated with a protease inhibitor-containing normal saline buffer, an organic solvent, Tirton X-containing PBS (phosphate buffer saline), SDS (sodium dodecyl sulfonate)-containing PBS, pancreatin-containing PBS, deoxyribonuclease-containing PBS, an EDTA (ethylene diamine tetraacetic acid) isotonic solution and ultrasonic waves, and the decellularized and decalcified bone material is obtained. Cancellous bones and cortical bones can be decellularized completely and simultaneously, the conditions are mild, damage to an ECM (extracellular matrix) is avoided, rapidness and stability are realized, and the obtained decellularized and decalcified bone material has the advantages of good biocompatibility, high plasticity, high biomechanical strength and the like and can be used for clinically repairing bone regeneration and repair disorder such as bone defects, nonunion and the like caused by various causes.

The present invention relates to one kind of biological rack material for articular cartilage and its preparation process. The porous rack material for articular cartilage is prepared through mixing natural cartilage matrix component with the cell, antigen and type I and type X collagen eliminated and type II gollagen, and serial physical and chemical treatments with sodium chloride crystal grain as pore forming agent. The rack material has the components the same as those of natural cartilage, high biomechanical strength, high porosity, proper degradation speed, use convenience and no influence on the body's and local immunity. The present invention may be used in the clinical repair of articular cartilage defect and in vivo and in vitro construction of tissue engineering cartilage.

A cell-removed heterogeneous bone matrix is prepared from the rib and extremity bone of pig through physical and chemical processing, and removing cells and the heterogeneous protein from tissue by use of proteolytic enzyme and Triton-X100. Its advantages are natural components and biologic chemical strength, low antigenicity, high compatibility and bone conductivity, and low cost.

The invention provides a tissue engineering meniscus scaffold and a preparation method thereof. The preparation method comprises the following steps: placing a polycaprolactone material into a nozzle of a fused deposition modeling three-dimensional printer to be heated to 120-140 DEG C, and preparing for printing at the air pressure of 600-1000 kPa; setting the printing speed of the fused deposition modeling three-dimensional printer to be 0.6-0.75 mm/s, the fiber diameter to be 300-320 [mu]m and the fiber interval to be 200-300 [mu]m and printing a tissue engineering meniscus scaffold; using cobalt-60 for irradiation and sterilization treatment. As the fused deposition modeling method is adopted to print the tissue engineering meniscus scaffold with an appropriate pore structure, an appropriate fiber diameter and appropriate biomechanical strength, the survival rate and the reproduction rate of inoculated cells and autologous in-growth cells of implanted regions are favorably increased, the fibrous cartilage differentiation rate can be increased, and newly generated meniscus tissues have favorable functions.

The invention discloses a method for preparing alpha-calcium sulfate hemihydrates (alpha-CSH) /beta-tertiary calcium phosphate (beta-TCP) porous granular-type composite artificial bones, which is characterized in that the alpha-CSH is directly synthesized on the surface and/or in the pores of the beta-TCP granules synchronously by the hydrothermal synthesis process and finally the alpha-CSH/beta-TCP porous granular-type artificial bones is prepared. The invention has the advantages that the preparation process is simpler, the preparation period can be effectively reduced, and the preparation efficiency is improved; the proportion between alpha-CSH and beta-TCP phases of the granular-type composite artificial bones can be effectively controlled, thereby controlling the pore structure of the artificial bone granules; meanwhile, the invention guarantees the in-situ solidifying performance and degrading performance of the composite artificial bones, controls the evenness of the product and the porosity and pore diameter of the beta-TCP granules, improves the bone-formation performance and degrading performance of the composite artificial bones and further improves the quality of the product, thereby finally obtaining the composite artificial bones having self-solidifying performance and controllable degradation and having a certain biomechanical strength. The invention has good application and market prospect.

The invention discloses a preparation method of a tendon conjunction bone decellularization material of a natural tissue source. The heel tendon conjunction bone tissue of any size of a mammal is treated by normal saline containing protease inhibitor, PBS containing protease inhibitor, a PBS buffer solution containing Triton X, a PBS buffer solution containing SDS and a PBS buffer solution containing DNA enzyme, and the tendon conjunction bone decellularization material is obtained. Complete decellularization treatment can be carried out on tendon cells and bone cells on tendon conjunction bone tissue and cartilage cells on the interface at the same time, conditions are mold, no ECM damage is caused, the process is rapid and stable, and the obtained tendon conjunction bone material has the advantages of being good in biocompatibility, strong in plasticity, high in biomechanical strength and the like, and can be used for repairing tendon degenerative diseases caused by various etiological factors clinically, tendon severe tear and tendon conjunction bone regeneration and repair obstacles.

The invention discloses a composite nanofiber film of a structure bionic skin extracellular matrix and a producing method and application thereof, and belongs to the field of biomedical composite materials. The method includes the following steps: selecting a main ingredient in the skin extracellular matrix as a material; and adopting an electrospinning technique, controlling different technical parameters, using a high-speed rotation roller (at the rotation speed of 500-3000 rpm), parallel plate electrodes horizontally arranged as a receiver, or using a near field electrostatic spinning machine to produce the composite nanofiber film having an ordered structure. The producing method is simple to implement; the produced composite nanofiber film has excellent biocompatibility and biomechanical strength, fibers intersect with each other at a certain angle, and woven basket-shaped structure bionic of collagenous fibers in natural skin extracellular matrix can be achieved; and the composite nanofiber film has good application prospect in skin wound surface dressings.

The invention discloses a three-dimensional porous sponge scaffold with a meniscus matrix source and a preparation method and an application. The method comprises the following steps: with meniscus as a raw material, removing cells through mechanical crushing treatment, acetic acid enzyme digestion and a differential centrifugation method to obtain meniscus extracellular matrix slurry; building a three-dimensional porous scaffold through freeze-drying; and carrying out crosslinked reinforcement on the scaffold through ultraviolet and chemical methods. The prepared three-dimensional porous sponge scaffold with the meniscus matrix source has a controllable microstructure, appropriate degradation rate, good biocompatibility and certain biomechanical strength, is beneficial to cell adhesion, uniform distribution of seed cells to enter into the scaffold and proliferation migration growth, is free of immunological rejection when implanted in vivo, can be widely applied to the field of meniscus and cartilage tissue engineering, and has a good clinical application prospect.

The invention discloses a method for preparing a bovine cortical bone grafting material, comprising the following steps: degreasing and decalcifying a cleaned bovine cortical bone block, immersing the bovine cortical bone block in an inhibitor of a bone morphogenetic protein degrading enzyme, then performing deproteinization, and finally obtaining the bovine cortical bone grafting material by freezing and drying. The preparation method has the advantages of simple steps, wide material source, low cost, low antigenicity of the obtained bone grafting material, strong bone formation inducibility, high biomechanical strength.

The invention discloses a composite scaffold. The composite scaffold is prepared from naringin, gelatin microspheres, hydroxyapatite and silk fibroin. The composite scaffold is prepared by the following steps: 1) dropwise adding a 0.5-1g/ml naringin solution into the gelatin microspheres, then standing at the temperature of 2 to 8 DEG C over night, and performing freeze drying to obtain a naringin/gelatin microsphere compound; 2) mixing the hydroxyapatite and the naringin/gelatin microsphere compound with a 3.5 to 10 percent by weight SF solution, injecting the mixed solution into a pore plate, then performing freeze drying on the sample to obtain an NG/GMs/HA/SF composite scaffold. The traditional SF/HAp composite scaffold is loaded with the traditional Chinese medicinal monomer, and encapsulated with gelatin microspheres conforming to a bone microenvironment, thereby promoting healing of OVF and enhancing the mechanical strength of the fractured vertebral body; the composite scaffoldhas high rheological property, plasticity, biomechanical strength and higher bone formation prompting performance, so that the composite scaffold can be applied to bone defect areas of different shapes, has a durable effect and higher bone tissue repair ability, and is convenient for large-scale popularization and application.

The invention provides application of an acellular cornea stroma lens in treating ophthalmic diseases, and relates to the technical field of tissue engineering. The acellular cornea stroma lens prepared according to the preparation method can effectively remove the cellular constituents in a cornea stroma lens, reduce the immunogenicity of the cornea stroma lens and improve the biomechanical strength of the cornea stroma lens; moreover, the original morphology and the transparency of the cornea stroma lens can be effectively kept, and the prepared acellular cornea stroma lens has the advantages of being higher in safety, better in transparency and more stable in performance. Therefore, the acellular cornea stroma lens can serve as a permanent implant lens and is used for treating the ophthalmic diseases, and a new treating method is provided for the correction of clinical high myopia, presbyopia and anisometropia.

The invention relates to the field of medical instruments and provides a stent. The stent comprises a stent body (11) and pore channels (12), wherein the pore channels (12) penetrate through the stent body (11). The stent can combine with vascular transplantation for treating femoral head necrosis, local biomechanical strength after the focus of infection is removed can be repaired, local microcirculatory system is rebuilt, and regeneration and repair of bone tissue are promoted.

The invention belongs to the technical field of tendon patch materials, and particularly relates to a bionic natural tendon-bone gradient interface patch material and a preparation method thereof. The patch material is medical nanofibers of which the surfaces are covered with calcium phosphate crystals, and the fiber arrangement mode of the nanofibers is gradually transited from uniaxial orientation arrangement at one end to irregular arrangement at the other end, and calcium phosphate crystals are gradually reduced from the irregular arrangement end to the uniaxial orientation arrangement end; and the preparation method comprises the following steps of preparing a uniaxial orientation nanofiber membrane by taking a medical fiber material and a near-infrared dye as raw materials; gradually converting uniaxial orientation arrangement into irregular arrangement through laser irradiation at different time; then vertically placing in a culture dish, enabling the end with the longest irradiation time to face downwards, and dropwise adding simulated body fluid to obtain the bionic natural tendon-bone gradient interface patch material. The patch material provided by the invention has orientation structure and mineralization dual-gradient change, has excellent elasticity and good mechanical properties, and can meet the biomechanical strength and compliance required by an in-vivo microenvironment.

The invention relates to a canine uterus whole-organ acellular matrix and a preparation method as well as the application of the canine uterus whole-organ acellular matrix. The canine uterus whole-organ acellular matrix is prepared by the following steps: selecting raw materials, pre-treating, removing cells, sterilizing and storing. The canine uterus whole-organ acellular matrix can be used for preparing particles, fluid compositions, gels and active peptides. The canine uterus whole-organ acellular matrix completely reserves the complex three-dimensional array of the natural uterus extracellular matrix and the horizontal-inside longitudinal-outside ultra-structural of the smooth muscle membrane; the canine uterus whole-organ acellular matrix also reserves the complete and non-leaking vascular matrix network as well as reserves a large number of biological active ingredients such as growth factors, hyaluronic acid, amino glucan and laminin, so that the canine uterus whole-organ acellular matrix has high affinity to the endometrial stem cells. Thus, the canine uterus whole-organ acellular matrix has the biomechanical strength and tenacity similar to those of the natural uterus; moreover, the canine uterus whole-organ acellular matrix is thorough in cell removal, free of obvious immune rejection and free of ethical and moral issues.

The invention belongs to the technical field of tissue engineering, and discloses a construction (preparation) method of a sericin/nano-hydroxyapatite tissue engineering bone graft containing bone morphogenetic protein-2 and an application thereof. The graft comprises growth factors, a scaffold material and optional seed cells, wherein the seed cells are adhered to the scaffold material, a cell carrier compound with a space structure and biological activity is formed, the scaffold material is a composite scaffold material composed of sericin (SS) and nano-hydroxyapatite (nHAP), the sericin wraps a growth factor containing human recombinant bone morphogenetic protein-2, and bone marrow mesenchymal stem cells are adhered to the composite scaffold material. The pore diameter, degradation speed and biomechanical strength of the scaffold can be adjusted according to the ratio of SS to nHAP. The constructed bionic artificial tissue engineering bone can be used for repairing bone grafts withlarge-section bone defects, and animal experiments prove that the bionic artificial tissue engineering bone can well repair the large-section bone defects.