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Application of acellular cornea stroma lens in treating ophthalmic diseases

A technology for corneal stroma and ophthalmic diseases, applied in the field of tissue engineering, can solve the problems of original shape, curvature change, poor predictability and safety, and high astigmatism, and achieve the effect of reducing immunogenicity, improving mechanical strength, and safety

Inactive Publication Date: 2017-10-10
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, there are also some problems in the use of animal-derived corneas, for example: (1) Although the histocompatibility of animal-derived corneas is good, if the removal of its own cells is not complete, there will be a risk of immune rejection; ( 2) In addition, although there are currently some methods for removing corneal stromal cells, the existing corneal decellularized matrix preparation process easily leads to corneal water absorption and swelling, resulting in changes in the arrangement or conformation of collagen fibers in the matrix, reducing the transparency of the corneal stroma, Cause changes in the original shape, curvature, etc.; (3) Moreover, traditional corneal lenses are usually made by cutting with a special keratome, and the process is relatively rough, which may cause the cutting surface to be not smooth enough, or the lens part is lost. Predictability and safety Poor, and the incidence of short-term complications such as astigmatism is high

Method used

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  • Application of acellular cornea stroma lens in treating ophthalmic diseases
  • Application of acellular cornea stroma lens in treating ophthalmic diseases
  • Application of acellular cornea stroma lens in treating ophthalmic diseases

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preparation example Construction

[0037] The invention provides the application of the decellularized corneal stroma lens in the treatment of ophthalmic diseases, wherein the preparation method of the decellularized corneal stroma lens comprises: performing cell lysing and cross-linking treatment on the corneal stroma lens with the accurate thickness of the cells in sequence, and then The decellularized corneal stroma lens is obtained after sterilization.

[0038] In a preferred embodiment, the ophthalmic disease is hyperopia.

[0039] In a preferred embodiment, the ophthalmic disease is anisometropia.

[0040] In a preferred embodiment, the ophthalmic disease is hyperopia.

[0041] In the preparation method of decellularized keratoma lens:

[0042] Wherein, the 0.1%-3% TritonX-100 involved in the method of cell lysis can be, for example, but not limited to 0.1% TritonX-100, 0.3% TritonX-100, 0.5% TritonX-100, 0.8% TritonX-100, 1 %TritonX-100, 1.5% TritonX-100, 2% TritonX-100, 2.5% TritonX-100 or 3% TritonX...

Embodiment 1

[0051] Embodiment 1 Preparation method of decellularized corneal stroma lens

[0052] Step (a), disinfection: soak the corneal stroma lens with accurate thickness of cells obtained by full femtosecond laser technology in physiological saline containing 0.01 mg / ml penicillin and 0.1 mg / ml streptomycin for 3 hours , and then rinsed with 0.9% saline;

[0053] Step (b), cell lysis: soak in 0.9% normal saline containing 0.5% TritonX-100 for 24 hours, then rinse with 0.9% normal saline for 96 hours;

[0054] Step (c), cross-linking: Soak in 0.9% physiological saline containing a cross-linking agent, then rinse with 0.9% physiological saline for 24 hours, and the cross-linking agent is 1-(3-dimethylaminopropyl)-3-ethane Carbodiimide (EDC); the mass ratio of the amount of cross-linking agent to the corneal stroma lens processed through cell lysis is 1:5;

[0055] Step (d), sterilizing: irradiating the crosslinked corneal lens with gamma rays, and the irradiation dose is 25kGy;

[0...

Embodiment 2

[0069] Embodiment 2 Preparation method of decellularized corneal stroma lens

[0070] Step (a), disinfection: Soak the corneal stroma lens with the accurate thickness of cells obtained by full femtosecond laser technology in physiological saline containing 0.05 mg / ml penicillin and 0.2 mg / ml streptomycin for 2 hours ,, and then rinsed with 0.9% saline;

[0071] Step (b), cell lysis: immerse in 0.9% normal saline containing 0.3% TritonX-100 for 48 hours, then rinse with 0.9% normal saline for 96 hours;

[0072] Step (c), cross-linking: soak in 0.9% physiological saline containing cross-linking agent, then rinse with 0.9% physiological saline for 24h, cross-linking agent N-hydroxyl sulfosuccinimide (NHS); cross-linking agent The mass ratio of the usage amount to the corneal stroma lens processed by cell lysis is 1:9;

[0073] Step (d), sterilizing: irradiating the crosslinked corneal lens with gamma rays, and the irradiation dose is 25kGy;

[0074] It should be noted that, in...

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Abstract

The invention provides application of an acellular cornea stroma lens in treating ophthalmic diseases, and relates to the technical field of tissue engineering. The acellular cornea stroma lens prepared according to the preparation method can effectively remove the cellular constituents in a cornea stroma lens, reduce the immunogenicity of the cornea stroma lens and improve the biomechanical strength of the cornea stroma lens; moreover, the original morphology and the transparency of the cornea stroma lens can be effectively kept, and the prepared acellular cornea stroma lens has the advantages of being higher in safety, better in transparency and more stable in performance. Therefore, the acellular cornea stroma lens can serve as a permanent implant lens and is used for treating the ophthalmic diseases, and a new treating method is provided for the correction of clinical high myopia, presbyopia and anisometropia.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to the application of a decellularized corneal stroma lens in the treatment of ophthalmic diseases. Background technique [0002] Hyperopia is a state of refraction behind the retina after parallel light rays enter the eye. When the refractive power of the eyeball is insufficient or the axial length of the eye is insufficient, hyperopia occurs. As a kind of ophthalmic disease, hyperopia greatly affects people's quality of life, especially as the age increases, the probability of hyperopia will also increase. [0003] Hyperopia is a common ametropic eye disease in clinical practice, and the correction of high hypermetropia has always been a difficult problem in refractive therapy. The current methods of hyperopia correction treatment mainly include glasses, contact lenses or refractive surgery. [0004] Presbyopia is a physiological phenomenon, not a pathological state, ...

Claims

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Application Information

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IPC IPC(8): A61F2/16A61L27/36
CPCA61F2/1602A61F2240/002A61L27/3604A61L27/3641A61L27/3687A61L27/3691A61L2430/16
Inventor 余克明庄菁曾晨光杨习锋梁丽金吴奕辉
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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