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Bionic natural tendon-bone gradient interface patch material and preparation method thereof

A tendon and patch technology, applied in the field of tendon patch materials, can solve problems such as the preparation method of tissue engineering tendon-bone interface patch materials with orientation structure and mineralization components that have not been reported, so as to reduce the healing of scars, Excellent elasticity and good tissue compatibility

Active Publication Date: 2021-08-13
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no reported tissue engineering tendon-bone interface patch materials and preparation methods with dual gradient changes in orientation structure and mineralization components

Method used

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  • Bionic natural tendon-bone gradient interface patch material and preparation method thereof
  • Bionic natural tendon-bone gradient interface patch material and preparation method thereof
  • Bionic natural tendon-bone gradient interface patch material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This embodiment relates to a preparation method of a bionic natural tendon-bone gradient interface patch material, wherein the medical fiber material is polyurethane, and the near-infrared dye is indocyanine green; the specific steps include:

[0034] (1) Accurately weigh 0.8g of polyurethane (PU) and 0.008g of indocyanine green (ICG) with an electronic balance, dissolve them in 10mL of hexafluoroisopropanol, stir overnight with a magnetic stirrer, and stir evenly to obtain PU / ICG Spinning liquid: adopt the electrospinning equipment of prior art to prepare PU / ICG uniaxially oriented nanofiber film, spinning parameter is: the flow rate of spinning liquid is respectively 0.013mL / min, and the distance between nozzle and cylinder is 15cm, The nozzle voltage is 18KV, the drum is grounded, the drum speed is 2500rpm, and the PU / ICG uniaxially oriented nanofiber film is obtained after 5 hours;

[0035] (2) Cut the PU / ICG uniaxially oriented nanofiber membrane obtained in step (...

Embodiment 2

[0043] This example is an experiment on the effect of the bionic natural tendon-bone gradient interface patch material prepared in Example 1 on cell morphology. Tendon stem cells were harvested at 1×10 4 The concentration of each / well was inoculated in a 24-well plate containing the patch material prepared in Example 1. After cultivating for 3 days, F-actin / DAPI staining was used to observe the cell morphology. The specific method is:

[0044] 1. The complete culture medium of tendon stem cells (volume percentage composition: 89% low-sugar DMEM + 10% fetal bovine serum + 1% penicillin / streptomycin) was cultivated for 3 days, the old culture medium was removed, and then phosphate-buffered saline (PBS) wash 3 times;

[0045] 2. Fix the tendon stem cells with 1 mL / well of 3% glutaraldehyde aqueous solution at room temperature for 10 min, remove the glutaraldehyde, and then wash 3 times with PBS;

[0046] 3. Permeabilize with 0.1% Triton X-100 (polyethylene glycol octylphenyl et...

Embodiment 3

[0053] This embodiment relates to the application experiment of the patch material of the bionic natural tendon-bone gradient interface prepared in Example 1, specifically:

[0054] First, construct the injury model, inject pentobarbital sodium (1.5%, 2mL / Kg) into the ear veins of 12 New Zealand white rabbits for general anesthesia, then fix the rabbits sideways on the operating table, and routinely pre-operatively prepare the skin and disinfect , spread a sterile drape, and infiltrate the shoulder with lidocaine for local anesthesia; make a percutaneous incision about 3 cm in length, and separate the subcutaneous fascia layer by layer. After exposing the humeral head, find the subscapularis tendon; between the tendon and bone At the connected position, the subscapularis tendon was cut 50% of the thickness; then the surface stump of the humeral head was cleaned; and then randomly divided into two groups, namely the experimental group and the control group, the experimental grou...

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Abstract

The invention belongs to the technical field of tendon patch materials, and particularly relates to a bionic natural tendon-bone gradient interface patch material and a preparation method thereof. The patch material is medical nanofibers of which the surfaces are covered with calcium phosphate crystals, and the fiber arrangement mode of the nanofibers is gradually transited from uniaxial orientation arrangement at one end to irregular arrangement at the other end, and calcium phosphate crystals are gradually reduced from the irregular arrangement end to the uniaxial orientation arrangement end; and the preparation method comprises the following steps of preparing a uniaxial orientation nanofiber membrane by taking a medical fiber material and a near-infrared dye as raw materials; gradually converting uniaxial orientation arrangement into irregular arrangement through laser irradiation at different time; then vertically placing in a culture dish, enabling the end with the longest irradiation time to face downwards, and dropwise adding simulated body fluid to obtain the bionic natural tendon-bone gradient interface patch material. The patch material provided by the invention has orientation structure and mineralization dual-gradient change, has excellent elasticity and good mechanical properties, and can meet the biomechanical strength and compliance required by an in-vivo microenvironment.

Description

Technical field: [0001] The invention belongs to the technical field of tendon patch materials, in particular to a patch material for a bionic natural tendon-bone gradient interface and a preparation method thereof. The patch material has dual gradient changes in orientation structure and mineralization components, and has good Biomechanical properties, degradation properties and cytocompatibility. Background technique: [0002] Tendon is an important component structure of the functional activities of all joints in the whole body and is a part of muscle. Muscles include muscle bellies and tendons. Tendons refer to the position where the end of the muscle is connected to the bone. When the muscles are forced to contract, the tendons can move the joints and realize the normal functional activities of the human body. The biological properties of tendons affect the contractility and sports performance of muscles to a certain extent. Tendon injury is a common disease, common o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/00A61L27/32A61L27/50A61L27/58
CPCA61L27/18A61L27/00A61L27/32A61L27/50A61L27/58A61L2430/10A61L2400/12A61L2300/412A61L2420/02C08L75/04
Inventor 吴桐于承浩于腾波王元非陈仁杰张小佩刘娜
Owner QINGDAO UNIV
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