6 results about "Cells bone" patented technology

The present invention deals with a cold-cleaning process for deriving a decellularized bone tissue sample or decellularized osteochondral tissue sample to be used as a medical device, or as a raw material.

In vitro method for producing meat by multiplying non-human fibroblasts, muscle cells, skeletal muscle stem cells and / or myosatellite cells by cultivation in serum-free medium, characterized in that the serum-free medium is free of blood serum and free of albumin, free of ROCK inhibitor, free of pro-inflammatory interleukins (IL), in particular free of IL-6, free of insulin-like growth factor (IGF) and free of vascular endothelial growth factor (VEGF), and the medium contains an inhibitor of the TGFβ signaling pathway which is an inhibitor of Smad2 / 3, and in that the medium contains TGFβ.

The application provides a targeted pathological B cell temperature-sensitive hydrogel bone repair material and a preparation method and application thereof, and belongs to the technical field of bone repair biomaterials. The targeted pathological B cell bone repair material HPCH / B3-Cu-Zn-MBGs temperature-sensitive hydrogel provided by the application is prepared by mixing hydroxypropyl chitin HPCH and B3-Cu-Zn-MBGs, and the mass ratio of the hydroxypropyl chitin HPCH and the B3-Cu-Zn-MBGs is 5:1-1:1. The HPCH / B3-Cu-Zn-MBGs provided by the application significantly improves joint swelling, reduces arthritis index, and reduces the clinical symptoms of RA through the comprehensive action thereof.

This invention discloses a bio-barrier membrane with guided bone regeneration (GBR) function and its construction method, belonging to the technical field of bone tissue regeneration and repair materials. The bio-barrier membrane is a composite structure formed by a decellularized periosteum and an extracellular matrix (ECM) cross-linked layer. Specifically, it is prepared by in-situ cross-linking of the decellularized periosteum with an acidic ECM solution. This bio-barrier membrane is a bilayer composite membrane that mimics the natural periosteum, synergistically exerting barrier, osteoconduction, and osteoinduction effects, significantly improving bone repair and regeneration.

Novel methods of creating personalized mesenchymal stem cells from pluripotent stem cells, including the utilization of extracellular matrix components, optionally seeded with exogenous differentiation factors for creation of a substrate for promotion of mesenchymal stem cell formation from pluripotent cell populations. Matrices useful for stimulation of differentiation include decellularized bone matrix, placental matrix, and adipose tissue derived matrix. Growth factors such as BMP2, BMP4, GDF-11, and stem cell factor can be added to said matrix. Stimulation of differentiation can be induced by exposure to conditioned media isolated from mesenchymal stem cells.