15 results about "Cells bone" patented technology

The invention relates to the technical field of biological materials, in particular to a local anticoagulation acellular bone matrix composite material and a preparation method thereof.The preparation method comprises the following steps that S1, bone tissue is obtained and treated, and an acellular bone matrix is prepared; s2, mixing an anticoagulant with the acellular bone matrix to form an anticoagulant compound; s3, mixing the anticoagulant compound with a biocompatible carrier to form a final composite material; and S4, preparing the composite material into an implant or a stent with a required shape through a molding and curing process. By arranging the acellular bone matrix, the natural structure and biological activity of the bone matrix are reserved to the maximum extent, and meanwhile, all cell components are removed, so that immunogenicity and pathogen transmission are reduced; by adding the anticoagulant, discomfort can be reduced after the local anticoagulant acellular bone matrix composite material is implanted into a human body, and the problems that in the prior art, an acellular bone matrix has the blood coagulation risk, and incompletely-treated bone tissue has immunological rejection are solved.

The invention provides a targeted pathological B cell temperature-sensitive hydrogel bone repair material as well as a preparation method and application thereof, and belongs to the technical field of bone repair biomaterials.The targeted pathological B cell bone repair material HPCH / B3-Cu-Zn-MBGs temperature-sensitive hydrogel provided by the invention is prepared by mixing hydroxypropyl chitin HPCH and B3-Cu-Zn-MBGs, the mass ratio of the hydroxypropyl chitin HPCH to the B3-Cu-Zn-MBGs is (5: 1) to (1: 1); according to the HPCH / B3-Cu-Zn-MBGs provided by the invention, the joint swelling is remarkably improved through the comprehensive action of the HPCH / B3-Cu-Zn-MBGs, the arthritis index is reduced, and the clinical symptoms of RA (rheumatoid arthritis) are reduced.

The present invention deals with a cold-cleaning process for deriving a decellularized bone tissue sample or decellularized osteochondral tissue sample to be used as a medical device, or as a raw material.

The present invention discloses a magnetoresponsive artificial periosteum and its preparation method and application, which includes magnetic drive layers at both ends in the length direction of the artificial periosteum and a stretching layer disposed between the magnetic drive layers; the magnetic drive layer is composed of a hydrogel formed by photocrosslinking gelatin methacrylate loaded with magnetic nanoparticles; the stretching layer is composed of a hydrogel formed by photocrosslinking after the composite of decellularized periosteum matrix methacrylate and gelatin methacrylate. The artificial periosteum of the present invention is close to the natural periosteum biological microenvironment and specific structure, not only having a natural bone-like three-dimensional network structure, which is beneficial to cell adhesion and growth; but also having magnetoresponsive ability, generating strain in the artificial periosteum through an external magnetic field, simulating the elasticity of the natural periosteum, and having osteoconduction and osteoinductive effects, which is beneficial to repairing damaged tissues and promoting bone regeneration. The artificial periosteum of the present invention can achieve the repair and regeneration of bone through external mechanical stimulation and has good application prospects.

In vitro method for producing meat by multiplying non-human fibroblasts, muscle cells, skeletal muscle stem cells and / or myosatellite cells by cultivation in serum-free medium, characterized in that the serum-free medium is free of blood serum and free of albumin, free of ROCK inhibitor, free of pro-inflammatory interleukins (IL), in particular free of IL-6, free of insulin-like growth factor (IGF) and free of vascular endothelial growth factor (VEGF), and the medium contains an inhibitor of the TGFβ signaling pathway which is an inhibitor of Smad2 / 3, and in that the medium contains TGFβ.

A medical device for in vitro diagnostics is presented for determining personalized therapeutic efficacy in bone cancer and bone metastases from other types of cancer. The invention is a hydrogel that simulates the bone extracellular matrix, comprising bicompatible polymers and micropulverized decellularized bone matrix. A bioink is also presented, which, together with this hydrogel, contains cellular elements obtained from various pathologies present in bone tissue. This mixture is used to create an in vitro diagnostic medical device, for which bioprinting is performed on an automated system. This in vitro diagnostic medical device allows for the evaluation of different chemotherapeutic and immunotherapeutic treatments to personalize the efficacy of the therapies recommended to the patient.

The application provides a targeted pathological B cell temperature-sensitive hydrogel bone repair material and a preparation method and application thereof, and belongs to the technical field of bone repair biomaterials. The targeted pathological B cell bone repair material HPCH / B3-Cu-Zn-MBGs temperature-sensitive hydrogel provided by the application is prepared by mixing hydroxypropyl chitin HPCH and B3-Cu-Zn-MBGs, and the mass ratio of the hydroxypropyl chitin HPCH and the B3-Cu-Zn-MBGs is 5:1-1:1. The HPCH / B3-Cu-Zn-MBGs provided by the application significantly improves joint swelling, reduces arthritis index, and reduces the clinical symptoms of RA through the comprehensive action thereof.

This invention discloses a bio-barrier membrane with guided bone regeneration (GBR) function and its construction method, belonging to the technical field of bone tissue regeneration and repair materials. The bio-barrier membrane is a composite structure formed by a decellularized periosteum and an extracellular matrix (ECM) cross-linked layer. Specifically, it is prepared by in-situ cross-linking of the decellularized periosteum with an acidic ECM solution. This bio-barrier membrane is a bilayer composite membrane that mimics the natural periosteum, synergistically exerting barrier, osteoconduction, and osteoinduction effects, significantly improving bone repair and regeneration.

The invention discloses a preparation method of liquid metal-based efficient magnetocaloric effect functional bone cement, which is characterized by comprising the following steps: step 1, preparing raw materials including liquid bone cement and liquid metal; 2, the liquid metal and the liquid bone cement refrigerated in advance serve as an inner phase and an outer phase of a coaxial needle tube respectively, the flow, time and temperature of the inner phase and the outer phase are set, the inner phase and the outer phase intersect to form functional bone cement through the coaxial shearing technology, then the liquid bone cement is solidified, and the final state is obtained. Liquid metal droplets are implanted into bone cement as required through a coaxial interface shearing technology, efficient and on-demand heating and accurate and controllable heating temperature can be realized under the action of a low-intensity alternating magnetic field, and the device has the characteristics of high pertinence, controllable heating temperature, rapid temperature rise and the like; the method has an excellent application prospect in cancer treatment after bone metastasis of medical cancer cells.

The present disclosure relates to a method using pluripotent stem cells (PSCs) to reconstitute axis development in vitro, and to a method for producing the same. The present disclosure provides three-dimensional cell aggregates called "axioloids" that are generated in vitro from pluripotent stem cells and are composed of mesodermal cells polarized along the anterior-posterior and apical-basolateral axes. The cell aggregates can reconstitute various aspects of somitogenesis and axis development, including axial elongation, segmentation, epithelial somitogenesis and patterning (the formation of one or more somite-like structures), and oscillation of the segmentation clock under somitogenic culture conditions. The axioloids can also be used to induce various cell lineages and functional cells, and can be used as a platform for modeling and reconstituting human embryonic development, disease, and evolution. The axioloids can be further utilized for, among other things, teratogenic and toxicological evaluation of chemicals, production and testing of cell therapy products, research of congenital and acquired human diseases, and evaluation of ongoing and future therapeutic approaches.

Novel methods of creating personalized mesenchymal stem cells from pluripotent stem cells, including the utilization of extracellular matrix components, optionally seeded with exogenous differentiation factors for creation of a substrate for promotion of mesenchymal stem cell formation from pluripotent cell populations. Matrices useful for stimulation of differentiation include decellularized bone matrix, placental matrix, and adipose tissue derived matrix. Growth factors such as BMP2, BMP4, GDF-11, and stem cell factor can be added to said matrix. Stimulation of differentiation can be induced by exposure to conditioned media isolated from mesenchymal stem cells.

The invention relates to a method for culturing animal cells and a medium for use in the method, wherein the cells are in particular induced pluripotent stem cells (iPSCs) and / or mesenchymal cells or mesenchymal stem cells, e.g., cardiac stem cells, fibroblasts, muscle cells, skeletal muscle stem cells, and / or myosatellite cells. The method is characterized in that the medium is serum-free and contains an inhibitor of the TGFβ signaling pathway.

The invention discloses a CRISPR / Cas9 library high-throughput screening method of lung cancer bone metastasis related genes, which comprises the following steps: step 1, constructing an sgRNA library of SLC transporter and ABC transporter related genes, and carrying out lentivirus packaging to obtain a plasmid library; step 2, constructing cells capable of stably expressing the sgRNA library; step 3, inoculating the lentivirus infected cells to the left ventricle of the thymus-free nude mouse; and step 4, extracting genome DNA, carrying out next-generation sequencing, and screening out candidate genes by comparing abundance changes of the sgRNA before and after. For the blank field of high-throughput screening of lung cancer bone metastasis related genes, cells infected with an SLC transporter and ABC transporter related gene sgRNA library are subjected to tumor formation through left ventricle injection for the first time, key genes for promoting bone metastasis of lung cancer tumor cells are screened, and the application range of a CRISPR / Cas9 library is widened.

The invention provides a component and structure recombined natural bone repair material and a preparation method thereof.The preparation method comprises the following steps that natural cortical bones are smashed, and cortical bone meal with the particle size being 300-500 micrometers is generated; performing degreasing treatment on the cortex bone meal by adopting a mixed solution of trichloromethane and methanol to obtain degreased bone meal; carrying out decellularization treatment on the degreased bone meal by utilizing the elution solution to obtain decellularized bone meal; washing the decellularized bone meal until the pH value is 7.3-7.5, and carrying out freeze-drying treatment to generate freeze-dried bone meal; grinding the freeze-dried bone meal again to generate nano bone meal; uniformly mixing polycaprolactone PCL and nano bone meal to form a bone meal solution; the bone meal solution is used for 3D printing treatment to generate a bone repair material similar to a natural cancellous bone, so that allogeneic cortical bones are fully utilized, xenogeneic bone decellularization and antigen removal are more thorough, and the generated bone repair material is good in biocompatibility, easy to attach and grow tissues and good in repair effect.