36 results about "Cultured meat" patented technology

The invention relates to a separation and purification method of high-purity porcine muscle stem cells, for research and production of cultured meat. The separation and purification method disclosed by the invention comprises the steps of obtaining sterile muscular tissue, efficiently digesting the muscular tissue to obtain a large quantity of muscle mononuclear cells, and finally performing steaming cell sorting to obtain the high-purity porcine muscle stem cells. The invention further discloses a method for efficiently digesting the porcine muscular tissue. By the method, the digestion speedand the digestion intensity can be increased, and a large quantity of muscle mononuclear cells can be obtained. When the purification method disclosed by the invention is applied, the porcine musclestem cells of which the purity exceeds 92% (by PAX7) can be stably obtained. The porcine muscle stem cells obtained through separation have efficient disintegration efficiency, and MYOSIN expression quantity can reach 85%-90%. When the method disclosed by the invention is applied, a large quantity of seed cell sources can be provided for research and actual production of the cultured meat.

The invention discloses a production mold for porous reticular cultured meat with regularly arranged micro-columns and a production method of porous muscle tissue based on the mold. Myogenic cells, type I collagen, a DMEM medium containing phenolic red and a sodium hydroxide solution are mixed, a mixture containing cells is prepared, the mixture containing the cells is added into the mold, and hydrogel muscle tissue is cultured at 37 DEG C after 2 hours in an incubator containing 5% carbon dioxide. A growth medium is added, after the hydrogel muscle tissue is cultured for 1-3 d, the growth medium is changed into a differential medium, after the hydrogel muscle tissue is cultured in the differential medium for 5-7 d, the porous reticular muscle tissue is prepared, so that the hydrogel muscle tissue can contract between the micro-columns to form muscle bundles, contracting space around the micro-columns due to the muscle bundles can increase the diffusion of nutrients to the cells, wastedischarge is facilitated, spatial pattern of mechanical tension is controlled to guide the partial three-dimensional cell arrangement, differentiation is promoted, and the production of larger in vitro, in line with industrial production of cultured meat is realized.

The invention is directed to a method for producing an edible composition, comprising incubating a three-dimensional porous scaffold and a plurality of cell types comprising: myoblasts or progenitor cells thereof, at least one type of extracellular (ECM)-secreting cell and endothelial cells or progenitor cells thereof, and inducing myoblasts differentiation into myotubes.

Modified cell lines and methods for use in the production of cultured meat are disclosed. The inventive methods provide for insertion of cell cycle regulatory genes or genes that encode for animal myoglobin into the genome of an animal cell to proliferate and flavor cell productions, followed by excising the inserted genes to terminate proliferation.

The invention belongs to the technical field of food processing, and discloses cell culture meat, a preparation method and applications thereof, the preparation method of the cell culture meat comprises the following steps: adding auxiliary materials, a flavor supplement and a color supplement to a culture meat raw material, and uniformly mixing; carrying out a 3D printing method on the obtained raw materials to obtain a cell culture meat product; wherein the addition amount of the flavor supplement is 1%-5% of that of the raw material for cultivating meat; wherein the addition amount of the color supplement is 0.2%-0.5% of that of the raw material for cultivating meat. According to the invention, the flavor supplement and the color supplement are added in the preparation process of the cell culture meat, and 3D printing is carried out, so that the cell culture meat without meat color and smell is endowed with ruddy color and meat flavor of fresh meat, the process is simple, and the industrial production of the product is convenient to realize; the flavor and color of the product can be improved, and a new thought is provided for engineering development of cultured meat; the development of the meat cultivation industry can be accelerated, the industrialization can be realized as soon as possible, and the market prospect is good.

The invention provides an improved culture medium for in-vitro amplification culture of cultured meat seed cells, namely currently optimal seed cells (muscle stem cells) of cultured meat. The improvedculture medium is a cell culture medium added with VC and derivatives thereof, cells with the number more than 1000 times that of a normal culture medium can be obtained in in-vitro amplification culture, and more importantly, the muscle stem cells are subjected to in-vitro long-term continuous passage and can still keep differentiation potential after the required cell number is reached. Therefore, the improved culture medium can help mass amplification culture of functional muscle stem cells in vitro, and better meets the large-scale requirements of cultured meat production on seed cells.

A method of producing a hybrid foodstuff is provided. The method comprises combining a plant-originated substance with an amount of cultured animal cells so as to enhance a meat organoleptic and/or meat nutritional property in the hybrid foodstuff, wherein the animal cells do not form a tissue, and wherein the amount is below 30% (w/w) of the hybrid foodstuff.

The invention discloses a health-care product for enhancing quality of cultured meat turtles, which is prepared from 1-4 parts of Bighead Atractylodes Rhizome, 2-5 parts of curcuma, 2-5 parts of angelica, 3-4 parts of Arillus Longan, 2-4 parts of Chinese wolfberry, 1-5 parts of propolis, 1-4 parts of wild chrysanthemum flower, 1-5 parts of retinervus luffae fructus, 1-6 parts of vitamin E, 2-4 parts of fish meal and 1-4 parts of cellulase. The raw materials are pulverized and uniformly mixed. The health-care product for enhancing quality of cultured meat turtles can effectively promote the growth of meat turtles, and the finished meat turtle product has favorable quality and better mouthfeel than common meat turtles.

The invention discloses a health-care product for enhancing quality of cultured meat turtles, which is prepared from 1-4 parts of hawthorn, 2-4 parts of honeysuckle flower, 1-4 parts of angelica, 1-5 parts of Arillus Longan, 2-3 parts of Chinese wolfberry, 2-4 parts of propolis, 2-6 parts of wild chrysanthemum flower, 1-2 parts of retinervus luffae fructus, 24 parts of vitamin E, 1-4 parts of fish meal and 4-6 parts of cellulase. The raw materials are pulverized and uniformly mixed. The health-care product for enhancing quality of cultured meat turtles can effectively promote the growth of meat turtles, and the finished meat turtle product has favorable quality and better mouthfeel than common meat turtles.

A protein hydrolysate composition derived from a microorganism, such as a chemoautotrophic microorganism, and methods of preparing and using the same are provided. The protein hydrolysate composition may be produced sustainably through fixation of carbon dioxide from biogenic or atmospheric sources. The protein hydrolysate composition finds use in supplementing culture media for serum-free culturing of animal cells as well as for growing other types of cells such as probiotics and lactic acid bacteria. Thus, the present disclosure provides sustainable, humane processes for culturing cells for pharmaceutical and nutraceutical application as well as for human consumption as a food ingredient or product, including cultured meat.

The embodiment of the invention provides a special culture device for 3D biological tissues and a preparation method of blocky cultured meat. The special culture device for 3D biological tissues comprises a 3D biological tissue culture tank used for placing 3D biological tissues, and a liquid storage tank used for containing a culture medium; the 3D biological tissue culture tank and the liquid storage tank are connected through a pipeline to form a circulation loop for the culture medium to circularly flow; a sealing plug is arranged at an opening of the 3D biological tissue culture tank; anda plurality of culture medium filling needles are arranged on the inner side of the sealing plug, and the culture medium filling needles penetrate through 3D biological tissues during use. The special culture device for the 3D biological tissues can effectively guarantee uniform growth and differentiation of cells in the 3D biological tissues, and is suitable for preparing blocky cultured meat. According to the preparation method of the blocky cultivated meat, the preparation scale of the animal cultivated meat can be remarkably increased, the high-quality blocky cultivated meat is truly prepared, and the blocky cultivated meat has the chewing taste of meat products.

The invention provides plant-based cultured meat prepared on the basis of a vegetable protein scaffold and a preparation method of the plant-based cultured meat. The method comprises the following steps: inoculating muscle-derived cells by using a textured vegetable protein scaffold, enabling the cells to perform adhesion growth on the vegetable protein scaffold, and performing induced differentiation by using differentiation culture to enable the cells to generate more muscle-related proteins, thereby preparing a cultured meat product which contains both vegetable proteins and animal proteins and has better meat quality. Therefore, the plant-based protein cultured meat has better meat quality, the defects of original products can be overcome, and on the other hand, the manufacturing cost of the cultured meat can be reduced by utilizing the plant protein scaffold.

The invention provides an improved culture medium for in-vitro enlarged culture of cultured meat seed cells, namely adipose tissue-derived stromal cells. The improved culture medium is a growth culture medium added with bFGF, the total number of cells obtained by culturing for 15 days by adopting the improved culture medium is 6 times of the total number of cells obtained in a control growth culture medium, and more importantly, the adipose tissue-derived stromal cells are massively amplified in vitro and can still keep relatively high differentiation capacity after the required cell number isreached. Therefore, the improved culture medium is helpful for obtaining a large number of functional adipose tissue-derived stromal cells in vitro, and better meets the requirements of cultured meatproduction on the number of seed cells and stem cell functions.

The invention provides an improved culture medium for short-term proliferation of cultured meat seed cells, which can accelerate the proliferation speed of the current most potential seed cells-muscle stem cells of cultured meat in short-term culture in vitro. The improved culture medium is a cell proliferation culture medium added with water-soluble vitamin E, the proliferation speed of the cells cultured by the improved proliferation culture medium in short-term culture in vitro is significantly improved, and the number of the cells is 1.2 times of that of the cells cultured by a normal culture medium; and the cells cultured by the improved proliferation culture medium added with the water-soluble vitamin E are induced and differentiated in a differentiation culture medium, so that the differentiation ability of the cells can be significantly improved, and more muscle proteins can be produced. Therefore, the improved proliferation culture medium can help to obtain a large number of cultured meat seed cells in short term in vitro, and plays a very beneficial role in the production of the cultured meat.

The invention discloses a cultivation method of meat-like Ganoderma lucidum. The method adopts materials as follows: coix seeds, sticky rice, red dates, Chinese wolfberry fruits, astragalus membranaceus, cordyceps sobolifera powder, honey, ephedra, tremella, agaric, Chinese yam, ginseng, rhodiola rosea, rhizoma atractylodis macrocephalae, hawthorn fruits, mulberries, mangoes, papaya powder, grapes, peaches and meat-like Ganoderma lucidum. The method disclosed by the invention has the beneficial effects that the rapid cultivation of the meat-like Ganoderma lucidum is realized, the production cycle is stable, and a meat-like Ganoderma lucidum product with high content of effective active substances and high nutritional and medicinal values can be obtained, so that the industrialized production becomes possible; and the growth state of the meat-like Ganoderma lucidum is improved, the cultured meat-like Ganoderma lucidum is good in appearance, good in shape and high in quality, the content of various beneficial active substances contained in the meat-like Ganoderma lucidum is increased, and the nutrition and health care effects of the meat-like Ganoderma lucidum are further improved.

The present disclosure relates to methods for enhancing cultured meat production, such as livestock-autonomous meat production. In certain aspects, the meat is any metazoan tissue or cell-derived comestible product intended for use as a comestible food or nutritional component by humans, companion animals, domesticated or captive animals whose carcasses are intended for comestible use, service animals, conserved animal species, animals used for experimental purposes, or cell cultures.

The invention discloses a compound feed for increasing the meat quality of cultured meat turtles. The compound feed is prepared from white fish powder, tylorrhynchus heterochaetus, residual entrails generated after treatment of urechis unicinctus, mussel, barnacle, fat, essential amino acid, delicious amino acid and a fatty liver prevention additive, wherein the fatty liver prevention additive isprepared from gralteloupia ramosissima polysaccharide, a poplar flower extract, taurine, phospholipid and choline chloride. The invention also discloses a preparation method for increasing the meat quality of the meat turtles. The compound feed disclosed by the invention has the beneficial effects that tylorrhynchus heterochaetus powder and the residual entrails generated after the treatment of the urechis unicinctus are used for replacing the white fish powder, grease which is rich in unsaturated fatty acid is used as a fat source, mineral elements and feed adhesives are provided by utilizingthe mussel and the barnacle, the fatty liver prevention additive is added, the components are complete, the nutrition is balanced, the palatability is good, decomposition, digestion and absorption ofthe fat are promoted, fatty liver disease is avoided, health growth of the meat turtles is promoted, the muscle quality is improved, and the culture benefit is increased.

The invention discloses a serum-free culture system and application thereof in cultured meat, and belongs to the technical field of bioengineering. The serum-free culture system can reduce dependenceof animal cell culture process on serum, and reduce influence of uncertain factors in serum on safety of animal cell culture. In addition, all exogenous additive components except basic culture mediumused in the serum-free culture system are completely synthesized, so that cost of the serum-free culture system can be effectively reduced, and large-scale application of the serum-free culture system is facilitated.

In order to overcome the defects of conventional scaffolds for adipogenic stem cell culture and the defects of flavor, nutrition, taste, juiciness and the like of existing cultured meat, the invention provides application of plant drawing protein in an adipogenic stem cell in-vitro culture scaffold and a preparation method of plant-based fat cultured meat. The method specifically comprises the following steps: S1, pretreatment of a plant wiredrawing protein scaffold: after plant wiredrawing protein absorbs water and expands, cutting the plant wiredrawing protein into appropriate sizes, drying, sterilizing, and soaking the plant wiredrawing protein scaffold in a growth culture medium overnight under an aseptic condition; s2, adding the adipogenic stem cells into a growth culture medium to prepare a cell suspension, inoculating the cell suspension onto a plant drawing protein scaffold, and carrying out multiplication culture; and S3, carrying out proliferation culture until the coverage rate of cells on the scaffold reaches 80-90%, carrying out induced differentiation culture until the cells are differentiated into fat cells, and finally preparing the plant-based fat culture meat with the animal meat flavor.