60 results about "Cultured meat" patented technology

The invention provides a preparation method of a cell culture three-dimensional scaffold and cell culture meat, which comprises the following steps: firstly, preparing a gelatin-carrageenan mixed solution and a gel precursor solution of transglutaminase, dividing into two parts, incubating at constant temperature to obtain two parts of composite hydrogel, randomly freeze-drying one part to obtain aerogel, and freeze-drying the aerogel to obtain the cell culture three-dimensional scaffold. Stacking on the other part and incubating at constant temperature again to obtain the prefabricated double-layer gel. The prefabricated double-layer gel is directionally frozen, ice crystals grow upwards from the bottom, freezing is stopped after the temperature is reduced to the set temperature, and the cell culture three-dimensional scaffold is obtained through drying. The stent adopts an ordered-disordered partition design, parallel micro-channels are formed in the lower layer by using a directional ice template method, and muscle fiber arrangement is simulated; a porous spongy structure is constructed on the upper layer by using a random ice template method, and is adapted to adipocyte proliferation. Two layers of interfaces are interpenetrated and communicated, natural mechanical gradients are combined, natural tissue characteristics are re-engraved, the traditional limitation is broken through, the cell co-culture efficiency and the cultured meat quality are improved, and industrialization is promoted.

The objective of the present invention is to provide a method for manufacturing powdered cultured meat that can increase cell differentiation efficiency and protein content with economic feasibility, and to provide powdered cultured meat with high physicochemical stability and transportability, as well as a food composition containing the same. The powdered cultured meat has a flavor similar to real meat and, as a high-protein food, has excellent utility in various food compositions or processed foods.

The present disclosure provides extruded or spun semi-permeable porous hollow fibers comprising covalent ester, thioester, and / or amide cross-linked polypeptides and methods of producing the same. The hollow fibers may be produced from proteins, protein extracts and / or protein isolates derived from plants, animals, bacteria, algae, archaea and / or fungi and, in certain embodiments, are intended to be suitable for human and / or animal ingestion. In some embodiments, the hollow fibers may be designed for producing cartridges compatible with existing and / or novel bioreactor platforms for containing cell cultures in cultured meat production.

The invention discloses a composite modifier and a method for improving the texture of meat paste of cultured meat, belongs to the technical field of novel food processing, and aims to solve the problems of loose structure, poor chewing feeling, insufficient water-retaining property and the like of the cultured meat. The method comprises the following steps: chopping and mixing a composite modifier containing soybean protein isolate and distarch phosphate in a mass ratio of (1-9): (9-1) with a culture meat paste base material at 4 DEG C or below according to a proportion of 5-15% of the total weight of the culture meat paste, then carrying out centrifugal degassing on the mixed meat paste, carrying out water bath heating gelation at 80-90 DEG C, and carrying out cooling storage at 2-6 DEG C, therefore, a stable gel network with enhanced hardness, elasticity and water binding capacity is constructed, and the gel network is mainly used for improving the texture quality and sensory acceptance of cultured meat products.

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

A new cell culture system capable of improving the efficiency in cell culture is provided. A cell culture system comprises a plurality of cell culture devices which are connected such that a culture medium moves through each of the cell culture devices, and a liquid level of the culture medium stored in each of the cell culture devices is controlled such that cells in at least one of the cell culture devices are in a first state in which the cells are immersed in the culture medium, while cells in the remaining cell culture devices are in a second state in which the cells are not immersed in the culture medium.

The present invention relates to: a cultured meat medium additive and a cultured meat medium composition that can induce muscle growth by differentiating muscle stem cells into muscle cells and inhibiting the expression of myostatin, which inhibits muscle growth; and a method for preparing cultured meat by using same. Specifically, the cultured meat medium additive and the cultured meat medium composition comprise laxogenin and 5-alpha hydroxy laxogenin.

The present invention relates to a single-phase, spherical edible microcarrier for the three-dimensional culture of stem cells intended for the preparation of cultured meat. The microcarrier comprises a homogeneous mixture of edible ionic polysaccharide (0.375-1.5%), edible protein (1-2.5%), edible polymer (0.025-0.1%) and water (95.9-98.6%). The invention also relates to a method for preparing these microcarriers by mixing three solutions: ionic polysaccharide in saline solution, edible protein in saline solution and edible polymer in water. The mixture is subjected to a process involving microspraying or electrospraying with calcium chloride in order to form spherical microcarriers of 50-300 micrometres. The design of the single-phase, spherical edible microcarrier simplifies the production of cultured meat by dispensing with the need for core-shell structures, thereby improving cell anchorage efficiency and facilitating cell recovery.

This invention relates to the fields of stem cell culture and serum-free culture media, specifically to a serum-free culture medium for the proliferation of bovine muscle stem cells and its applications. The serum-free culture medium is based on a serum-free basal culture medium, to which food-derived small molecule compounds are added. Through further combination optimization, four food-derived small molecule compounds with synergistic effects have been obtained. This serum-free culture medium can significantly promote the proliferation of bovine muscle stem cells, effectively maintain cell proliferation activity, and cells passaged using this serum-free culture medium can still maintain good differentiation potential. This provides a stable, sufficient, and safe cell source for the industrial production of cultured meat, which is beneficial for the large-scale production of bovine muscle stem cells.

A substrate for culturing cells that comprises a modified prolamin having a net positive charge is provided. The modified prolamin may be a prolamin that has been modified through amidation or esterification, such as the addition of a methyl-ester group or an ethanolamine group. The modified prolamin is animal-free may be food-grade and / or may be free of cross-linkers. The modified prolamin may be in a coating that can be applied to a surface for culturing adherent cells, such as a microcarrier (e.g., beads) or well plates. Substrates coated with the modified prolamin are able to support cell expansion and can be used for food-grade applications such as cultured meat.

The present invention aims to provide a cell culture substrate suitable for cell culture. In particular, it aims to provide a cell culture substrate that can efficiently produce cultured meat. [Solution] The cell culture substrate according to the present invention comprises a three-dimensional structure composed of proteins that facilitate cell adhesion and lipids that provide a hydrophobic environment conducive to cell adhesion. Therefore, animal cells can be cultured using a cell culture carrier equipped with this three-dimensional structure. Furthermore, in the cell culture substrate of the present invention, the sum of the dry mass of proteins and lipids in the dry mass of the cell culture substrate is 80% by mass or more, so animal cells adhere easily to the cell culture substrate, preventing unintended inhibition of culture. Based on the above, the cell culture substrate according to the present invention is a cell culture substrate suitable for cell culture.

The present invention relates to a composition for promoting differentiation of muscle stem cells into myotubes, comprising a compound that promotes lipid metabolism or cell differentiation, for example, ethanolamine or a functional equivalent thereof, and to a method for differentiating muscle stem cells using same. More specifically, the present invention provides a composition for promoting differentiation of muscle stem cells into myotubes, comprising only ethanolamine, a composition for a muscle stem cell culture medium comprising same, a method for differentiating muscle stem cells into myotubes, using the composition for promoting differentiation or the composition for a culture medium, a myotube culture obtained by the differentiation method, and a cultured meat and food composition comprising same.

The purpose of the present invention is to provide a culture medium in which cells to be used for producing a cultured meat can be grown, by adding thereto a food material as an additive. As a result

The invention discloses a co-culture method of cell culture meat and application of the co-culture method in improving meat quality and flavor. According to the invention, through two-stage differentiation, muscle precursor cells and fat precursor cells are respectively subjected to proliferation culture, then are separately subjected to primary differentiation, and are mixed together for co-culture, and meanwhile, second-stage continuous differentiation is carried out; the problem that differentiation of muscle cells and fat cells cannot be effectively and simultaneously differentiated under the co-culture condition due to different requirements of differentiation of muscle cells and fat cells on the serum environment is solved. In addition, the invention also provides an objective standard for evaluating the flavor quality of cell culture meat generated by co-culture. The cell culture meat with different flavors can be obtained by adjusting the proportion of the co-culture cells through the co-culture method, and the flavor quality of the cell culture meat from other sources can be evaluated and detected through the amino acid detection means.

Provided is a method for inducing muscle-derived differentiation of porcine pluripotent stem cells, which does not involve transgenosis and is free of serum addition in the whole course, and also provided is a method for preparing cell cultured meat (CM) based on the muscle-derived differentiation induction method.

Provided are: an improved culture medium composition which can be used for culturing pluripotent stem cells of mammals, preferably for establishing and maintaining pluripotent stem cells of mammals useful for producing cultured meat for cattle, pigs, or the like; and a culture method using the culture medium composition. The culture method according to the present invention comprises a step of culturing pluripotent stem cells of mammals in a culture medium composition, the culture medium composition being a culture medium composition for culturing pluripotent stem cells of mammals, the culture medium composition being a serum-free culture medium containing (1) a CDK8 / 19 inhibitor, (2) a ROCK inhibitor, (3) a SMAD activator, and (4) a STAT3 activator.

The present invention relates to a culture medium composition for promoting muscle cell differentiation, the culture medium composition comprising quercetin as an active ingredient. It was found that quercetin promotes the differentiation of muscle cells by expressing muscle differentiation-related factors and promoting creatine kinase activity, and thus can be effectively used as a composition for muscle cell differentiation or a medium composition for producing cultured meat.

The invention relates to the technical field of biology, and provides a cell culture meat food safety evaluation method based on a nematode model, and the nematode model is a caenorhabditis elegans model, comprises individuals at the L1 stage and the L4 stage development stage, and is used for establishing a chronic exposure model and an acute exposure model. Biological parameters are obtained by measuring the model of the cell culture meat protein after ingestion of the marker, and the parameters are compared and analyzed with parameters of a control group, so that the food safety of the cell culture meat protein is evaluated. According to the method, caenorhabditis elegans is taken as a model organism, the provided cell culture meat food safety evaluation method is simple and rapid, low in cost, short in test period and good in repeatability, early warning is provided for potential toxic components in food, and the safety evaluation process is accelerated.

Provided is a technique with which it is possible to improve the proliferation rate of cells in culture in which microcarriers are used. A cell line is established by introducing, into bovine progenitor cells, the following four genes: CDK4 (R24C), TERT, CCND1, and p53 that lacks a DNA binding domain.

This invention discloses a method for preparing softened grains and a method for preparing chicken-flavored rice from softened grains, belonging to the field of bio-cultured meat technology. The method for preparing softened grains includes a method for preparing chicken-flavored grains and collagen incubation; the method for preparing chicken-flavored rice from softened grains includes chicken myoblast inoculation, chicken myoblast culture, and harvesting the chicken-flavored rice. The production process of this invention does not rely on the use of commercial microcarriers, significantly reducing the complexity and cost of the production process; the production process of this invention is mature and controllable, enabling stable product quality in large-scale production; it is green and environmentally friendly, with no resource waste and no animal cruelty; by not using commercial microcarriers, not only is the cost of raw materials reduced, but the production process is also simplified, making the entire production process more efficient and economical; by using biomaterials such as fish collagen, the nutritional value and biocompatibility of the product are improved, making the final product more competitive in the market.

This invention relates to the field of bioengineering technology, specifically to an extracellular vesicle from kelp, its preparation method, and its applications. This invention provides a method for efficiently isolating extracellular vesicles with specific biological activities from kelp, and an application scheme for these vesicles to promote the proliferation, migration, and maintenance of stemness of large yellow croaker muscle stem cells under low serum conditions. This overcomes the problems of high cost and limited functionality of fetal bovine serum as a substitute in existing cell-cultured meat technologies, providing a novel culture medium supplement component that is stable in origin, low in cost, and has a clear effect.

This invention belongs to the field of stem cell and animal cell-cultured meat technology, specifically relating to a 3D-printed cell-cultured fish meat and its preparation method. The method includes the following steps: first, stem cells are expanded through suspension culture using edible microcarriers; next, the cell-laden microcarriers are combined with 3D printing technology to perform cell stacking, forming a 3D-printed cultured fish meat construct; finally, the 3D-printed cultured fish meat is shaped to obtain the finished product. This invention provides a 3D-printed cell-cultured fish meat and its preparation method, which effectively simulates the contents of the diaphragm and sarcomeres by utilizing surface-differentiated myotubes and fat microcarriers as the main raw materials for bio-ink, greatly improving the quality of the 3D-printed cell-cultured fish meat.

Disclosed herein are edible fiber scaffolds, methods of generating an edible fiber scaffold, devices for generating modified edible fiber scaffolds, and systems for producing strands of cultured meat tissue. The edible fiber scaffolds may comprise, for example, soy protein isolate (SPI) and at least one additive, e.g., glycerol or pectin. The glycerol or pectin may facilitate advantageous physical properties that mimic natural meat products. This disclosure also provides methods, systems, and devices for facilitating the culture of cells on the edible fiber scaffolds.

An edible scaffold for cultured meat production. The scaffold comprises a polysaccharide and a plant-based protein formed of fibers, microcarriers, or other structural elements. The structural elements can comprise a diameter between about 1 μm to about 1000 μm. The scaffold can be formed via extrusion or an electrohydrodynamic technique. The scaffold further undergoes a surface modification treatment configured to facilitate attachment by cells. The scaffold can be used to culture myoblasts and other cells for the production of a meat product. The scaffold is incorporated into and ultimately a component of the resulting meat product.

This invention belongs to the field of cultured meat and relates to a method for constructing a cultured meat scaffold. The invention involves preheating a deionized aqueous solution of soybean protein and then cooling it to room temperature to obtain a soybean protein solution; dissolving fish gelatin in deionized water to obtain a fish gelatin solution; mixing the soybean protein solution and the fish gelatin solution, stirring until homogeneous to obtain a mixed solution; then adding glutaminase solution; heating and incubating to inactivate the enzyme; and after full hydration, obtaining a gel scaffold, which is then ready for consumption as a dual-protein cultured meat scaffold. Preheating and enzymatic cross-linking are used to regulate the dual-protein gel network, reducing its heterogeneity and improving its stability and strength. The dual-protein gel scaffold possesses excellent mechanical properties, a uniform network structure, and biocompatibility, which can improve the adhesion, proliferation, and spreading area of ​​porcine skeletal muscle satellite cells, while simultaneously meeting the requirements for myoblastic differentiation.

The invention provides a low-cost, safe and serum-free culture medium for in-vitro efficient suspension culture of porcine muscle stem cells and application of the serum-free culture medium. Comprising cell culture supplementary factors and a basic culture medium, the cell culture supplementary factors comprise zinc sulfate, L-ascorbic acid, bFGF, IGF-1, LIF, EGF, cortisol, HGF, alpha-tocopherol, PVA, beta-cyclodextrin, methyl cellulose and a yeast extract. The efficient suspension amplification of the porcine muscle stem cells under serum-free and carrier-free conditions is realized by adding factors in a simplified and optimized manner and adding cell culture supplementary factors with specific types and concentrations. According to the culture medium disclosed by the invention, the addition cost of exogenous factors is remarkably reduced, various potential risk substances are avoided, the food safety and economy of the culture medium are improved while efficient growth of cells is ensured and the process is simplified, and an important technical and raw material basis is provided for industrial production of cell culture meat.

This invention provides a technology that can improve the proliferation rate of cells in cultures using microcarriers. [Solution] The cell line is established by introducing the following four genes: CDK4(R24C), TERT, CCND1, and p53 lacking the DNA binding domain into bovine progenitor cells.

The present invention relates to a process for the production of in vitro engineered tissues, also known in the art as cultured meat, cultivated meat, cell based meat, cellular meat and / or clean meat, and a device for the production of the same. The process comprises the steps of: loading sterilizable 3D scaffolds into a seeding chamber, sterilization of the scaffolds in the seeding chamber, seeding the scaffolds by loading a first volume of culture medium into the seeding chamber, wherein the first volume of culture medium has a density of cells in suspension of 5,000 to 25,000 cell / cm2, more preferably from 10,000 to 16,000 cm2 such that the scaffolds are immersed in the culture medium, leaving the scaffolds and the first volume of culture medium in the seeding chamber for a period of 2-24 hours at 18-37° C., loading a second volume of culture medium into a bioreactor, the second volume being greater than the first volume of the culture medium wherein an incubation position of one or more movable grids inside the bioreactor confines the scaffolds to the second volume of the culture medium such that they remain immersed during an incubation step, wherein the scaffolds and the second volume of culture medium are incubated in the bioreactor for a period of 10-60 days at 18-37° C. and at a pH between 6.5-8.0, more preferably 7.0-7.4. The invention also relates to a device for the production of cultured meat.

The present disclosure provides a meat culture construct (400) for a meat culturing system, the meat culturing system having a housing adapted to receive the meat culture construct. The meat culture construct comprises a plurality of fibres (412) and an end support (404) in which ends of the plurality of fibres are embedded such that the plurality of fibres extend from the end support and into the housing when the meat culture construct is received in the housing of the meat culturing system. At a plane through the end support the plurality of fibres are arranged in a fibre distribution defined by an envelope (414) containing the plurality of fibres. The envelope has a first dimension (416) and a second dimension (418), and a ratio of the first dimension to the second dimension is at least 2:1 in order to enhance fluid access to fibres within the fibre distribution. Also disclosed herein is a harvesting system, a meat culturing system, a method of seeding a meat culture construct, a method of harvesting cultured meat, and a meat of culturing meat.

This invention relates to the field of cell biotechnology, specifically to a method for large-scale preparation of cultured meat using a pilot-scale standardized bioreactor. The method includes the following steps: preparing photocurable microcarriers using gelatin and a photoinitiator; mixing large yellow croaker myoblasts, large yellow croaker preadipocytes, and the photocurable microcarriers, and then inoculating the mixture into a pilot-scale standardized bioreactor for culture; collecting the cell-bearing microcarriers cultured in the pilot-scale standardized bioreactor; and processing the cell-bearing microcarriers using a mold to prepare cultured meat. This invention uses large yellow croaker myoblasts, large yellow croaker preadipocytes, and photocurable microcarriers as experimental materials. Through a specific culture process, the pilot-scale standardized bioreactor is set up. By utilizing real-time monitoring and control of the culture conditions in the pilot-scale standardized bioreactor, the suspension co-culture of two types of large yellow croaker stem cells on photocurable microcarriers is achieved. Large-sized cultured meat is then prepared through mold processing.