The invention discloses a production mold for porous reticular
cultured meat with regularly arranged micro-columns and a production method of porous
muscle tissue based on the mold. Myogenic cells,
type I collagen, a DMEM medium containing phenolic red and a
sodium hydroxide solution are mixed, a mixture containing cells is prepared, the mixture containing the cells is added into the mold, and hydrogel
muscle tissue is cultured at 37 DEG C after 2 hours in an
incubator containing 5%
carbon dioxide. A
growth medium is added, after the hydrogel
muscle tissue is cultured for 1-3 d, the
growth medium is changed into a differential medium, after the hydrogel
muscle tissue is cultured in the differential medium for 5-7 d, the porous reticular
muscle tissue is prepared, so that the hydrogel
muscle tissue can contract between the micro-columns to form muscle bundles, contracting space around the micro-columns due to the muscle bundles can increase the
diffusion of nutrients to the cells, wastedischarge is facilitated, spatial pattern of
mechanical tension is controlled to guide the partial three-dimensional
cell arrangement, differentiation is promoted, and the production of larger
in vitro, in line with industrial production of
cultured meat is realized.